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基于DNA条形码及ITS2二级结构鉴定白头翁及其易混伪品

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中医药信息 2024年第10期41卷 9-13页

作者：张治东郭春云郭阿林汤星辰方鑫杰王孟虎亳州学院安徽亳州236800 阜阳职业技术学院安徽阜阳236031 河南科技学院河南新乡453003

目的:基于DNA条形码及ITS2二级结构鉴定白头翁及其易混伪品(秋牡丹、火绒草、委陵菜),从而提高白头翁材鉴定的准确性和效率。方法:提取白头翁及易混伪品植物(秋牡丹、火绒草、委陵菜)的DNA,进行PCR扩增并测序,设计特异性引物,并采用特... 详细信息

目的:基于DNA条形码及ITS2二级结构鉴定白头翁及其易混伪品(秋牡丹、火绒草、委陵菜),从而提高白头翁材鉴定的准确性和效率。方法:提取白头翁及易混伪品植物(秋牡丹、火绒草、委陵菜)的DNA,进行PCR扩增并测序,设计特异性引物,并采用特定的分子标记,提取DNA条形码和ITS2(内转录间隔区2)区域的二级结构,使用MEGA11.0软件对白头翁及易混伪品植物进行分析研究。结果:Neighbor-Joining(NJ)树显示白头翁、秋牡丹、火绒草、委陵菜分别独立聚为一支,且白头翁、秋牡丹、火绒草、委陵菜的二级结构在整体骨架上有明显不同,因此根据ITS2碱基序列能够对白头翁及其混伪品进行鉴定。结论:利用DNA条形码及ITS2二级结构能够准确对白头翁及易混伪品(秋牡丹、火绒草、委陵菜)进行鉴定。

关键词：白头翁 ITS2 NJ树二级结构鉴定

Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis

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Neural Regeneration Research 2024年第3期19卷 671-679页

作者： chunyun Liu Shangde guo Rong Liu Minfang guo Qing Wang Zhi Chai Baoguo Xiao Cungen Ma Institute of Brain Science Shanxi Datong UniversityDatongShanxi ProvinceChina The Key Research Laboratory of Benefiting Qi for Acting Blood Cireulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine/Research Center of Neurobiology Shanxi University of Chinese MedicineTaiyuanShanxi ProvinceChina Institute of Neurology Huashan HospitalInstitutes of Brain Science and State Key Laboratory of Medical NeurobiologyFudan UniversityShanghaiChina

Multiple sclerosis is characterized by demyelination and neuronal loss caused by inflammatory cell activation and infiltration into the central nervous *** polarization plays an important role in the pathogenesis of experimental autoimmune encephalomyelitis,a traditional experimental model of multiple *** study investigated the effect of Fasudil on macrophages and examined the therapeutic potential of Fasudil-modified macrophages in experimental autoimmune *** found that Fasudil induced the conversion of macrophages from the pro-inflammatory M1 type to the anti-inflammatory M2 type,as shown by reduced expression of inducible nitric oxide synthase/nitric oxide,interleukin-12,and CD16/32 and increased expression of arginase-1,interleukin-10,CD14,and CD206,which was linked to inhibition of Rho kinase activity,decreased expression of toll-like receptors,nuclear factor-κB,and components of the mitogen-activated protein kinase signaling pathway,and generation of the pro-inflammatory cytokines tumor necrosis factor-α,interleukin-1β,and ***,Fasudil-modified macrophages effectively decreased the impact of experimental autoimmune encephalomyelitis,resulting in later onset of disease,lower symptom scores,less weight loss,and reduced demyelination compared with unmodified *** addition,Fasudil-modified macrophages decreased interleukin-17 expression on CD4^(+)T cells and CD16/32,inducible nitric oxide synthase,and interleukin-12 expression on F4/80^(+)macrophages,as well as increasing interleukin-10 expression on CD4^(+)T cells and arginase-1,CD206,and interleukin-10 expression on F4/80^(+)macrophages,which improved immune regulation and reduced *** findings suggest that Fasudil-modified macrophages may help treat experimental autoimmune encephalomyelitis by inducing M2 macrophage polarization and inhibiting the inflammatory response,thereby providing new insight into cell immunotherapy for m

关键词： anti-inflammatory experimental autoimmune encephalomyelitis Fasudil macrophage multiple sclerosis pro-inflammatory Rho kinase

A secreted salivary effector from Riptortus pedestris impairs soybean defense through modulating phytohormone signaling pathways

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Insect Science 2023年第6期30卷 1637-1647页

作者： Yue Huang Biao Hu Zhongyan Wei Shiqi Shan chunyun guo Hehong Zhang Yanjun Li Jianping Chen Xue Kang Haijian Huang Zongtao Sun State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang ProvinceInstitute of Plant VirologyNingbo UniversityNingboZhejiang ProvinceChina Institute of Drug Discovery Technology Ningbo UniversityNingboZhejiang ProvinceChina

Riptortus pedestris(Fabricius),one of the major piercing-sucking insects in soybeans,causes delayed plant senescence and abnormal pods,known as staygreen *** research has shown that direct feeding of this insect is the major cause of soybean staygreen ***,it remains unclear whether *** salivary proteins play vital roles in insect ***,we found that 4 secretory salivary proteins can induce cell death in Nicotiana benthamiana by transient heterologous *** cell death induced by Rp2155 relies on the nucleotide-binding leucine-rich repeat helper,***-specificity assays indicated that Rp2155 is specifically expressed in the salivary gland of *** and is significantly induced during insect *** expression of salicylic acid(SA)-,jasmonic acid(JA)-related genes was increased in soybean when fed by Rp2155-silenced *** importantly,soybean staygreen symptoms caused by *** were significantly alleviated when Rp2155 was ***,these results suggest that the salivary effector Rp2155 is involved in promoting insect infestation by suppressing the JA and SA pathways,and it can be considered as a potential RNA interference target for insect control.

关键词： cell death Riptortus pedestris salivary proteins soybean staygreen

Acupuncture Treating Heart Disease Based on Eight Palaces or Eight Veins <br/>—Mathematical Reasoning of Treatment Principle Based on Yin Yang Wu Xing Theory in Traditional Chinese Medicine (IV)

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Chinese Medicine 2021年第3期12卷 47-117页

作者： Yingshan Zhang Yan Li chunyun guo School of Statistics Faculty of Economics and Management East China Normal University Shanghai China College of Acupuncture and Massage Henan University of Traditional Chinese Medicine Zhengzhou China Traditional Chinese Medicine Acupuncture and Moxibustion Clinic Zhengzhou China

Theory of eight palaces (八宫) or eight veins (八脉) is useful in understanding the basic sick subsystems that may be to fall ills as the root-cause. By using mathematical reasoning based on Yin Yang Wu Xing Theory in Traditional Chinese Medicine (TCM), this paper demonstrates the treatment principle: “Even if all changed, it is hard to change one’s nature” (江山易改, 本性难移). The eight palaces or eight veins can be used to diagnose basic sick subsystems that may be to fall ills as the root-cause. The first or second transfer law of human body energies of the eight palaces or eight veins according to the different blood pH values of a human body whether in the normal range or not, respectively, assuming that the range of blood pH value is divided into four parts from small to large. Both second and third are for a healthy human body. Both the root-cause and symptoms come from the first transfer law of human body energies. And both first and fourth are for an unhealthy human body. Both the root

关键词： Traditional Chinese Medicine (TCM) Yin Yang Wu Xing Theory Steady Multilateral Systems Incompatibility Relations Side Effects Medical and Drug Resistance Problem

Acupuncture Treating Heart Diseases Based on Sixty JiaZi and Eight Palaces <br/>—Mathematical Reasoning of Treatment Principle Based on Yin Yang Wu Xing Theory in Traditional Chinese Medicine (V)

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Chinese Medicine 2021年第3期12卷 118-184页

Theory of Sixty JiaZi (60 甲子) is useful in understanding a human body disease. By using mathematical reasoning based on Yin Yang Wu Xing Theory in Traditional Chinese Medicine (TCM), this paper demonstrates the treatment principle: “seize the momentum of development, hasten lucky avoids disaster” (顺势而为, 趋吉避凶). Sixty JiaZi is hidden behind eight palaces. The first or second transfer law of human body energies of Sixty JiaZi changes according to the different blood pH values of a human body whether in the normal range or not, respectively, assuming that the range of blood pH values of a human body is divided into four parts from small to large. Both second and third are for a healthy human body. Both the root-cause and symptoms come from the first transfer law of human body energies. And both first and fourth are for an unhealthy human body. Both the root-cause and symptoms come from the second transfer law of human body energies. The treating disease of a human body should protect and maintain the balance of two incompatibility relations: the loving relationship and the killing relationship. As an application,

关键词： Traditional Chinese Medicine (TCM) Yin Yang Wu Xing Theory Steady Multilateral Systems Incompatibility Relations Side Effects Medical and Drug Resistance Problem

Identification of a von Willebrand factor type A protein affecting both grain and flag leaf morphologies in wheat

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Science China(Life Sciences) 2024年第10期67卷 2283-2286页

作者： chunyun Zhou Hongchun Xiong Yong Jia Huijun guo Meiyu Fu Yongdun Xie Linshu Zhao Jiayu Gu Huiyuan Li Yuting Li Peiyong Xin Jinfang Chu Chengdao Li Luxiang Liu State Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Western Crop Genetics Alliance College of ScienceHealthEngineering and EducationMurdoch UniversityPerthWA 6150Australia National Centre for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Dear Editor,The world faces unprecedented challenges for crop yield improvement to feed an expanding human population under limited agricultural resources and an increasingly erratic climate(Wheeler and von Braun,2013).Bread wheat is one of the most widely cultivated cereal crops,with both grain and flag leaf morphologies determining final yield potential(Xie et al.,2015;Zanella et al.,2023).Candidate genes affecting these traits are therefore highly desirable targets for breeding programs.

关键词： breeding crops cultivated

旅游绅士化影响下的乡村空间重构及其驱动机制——以徐州市汉王村为例

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地理科学进展 2024年第5期43卷 966-980页

作者：郭斯琪史春云钱月祥李帆江苏师范大学地理测绘与城乡规划学院江苏徐州221116 苏州科技大学地理科学与测绘工程学院江苏苏州215009

旅游驱动下的乡村绅士化作为乡村空间价值重塑的重要表现形式之一,在乡村振兴背景下受到极大关注。论文从乡村旅游绅士化影响效应这一视角切入,依据实地调研数据及空间解译结果,剖析徐州市汉王村在旅游绅士化发展不同时期空间所呈现出... 详细信息

旅游驱动下的乡村绅士化作为乡村空间价值重塑的重要表现形式之一,在乡村振兴背景下受到极大关注。论文从乡村旅游绅士化影响效应这一视角切入,依据实地调研数据及空间解译结果,剖析徐州市汉王村在旅游绅士化发展不同时期空间所呈现出的重构特征,并结合半结构式访谈数据,基于行动者网络理论,对空间重构机制进行分析。研究表明:①依据旅游建设和发展历程,可将汉王村旅游绅士化进程划分为传统农业转型、观光农业开发、乡村旅游发展和文旅融合转向4个阶段,不同阶段空间重构呈现出不同特征;②旅游驱动下的乡村绅士化过程改变了汉王村的用地分布、产业结构、人口构成和文化氛围,缓解了乡村空心化的部分问题,并对空间产生深远影响;③各级政府、村民、游客、投资商、艺术从业者、旅游资源等异质行动者利益目标的转变以及彼此间的联结与合作对汉王村空间重构产生作用。研究可为理解旅游驱动乡村振兴发展、引发乡村绅士化效应与破解空心村难题提供理论与实证参考。

关键词：乡村空间重构乡村旅游绅士化驱动机制行动者网络徐州市汉王村

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均聚聚丙烯在干热环境中耐候性能及结构表征

高分子材料科学与工程 2016年第11期32卷 81-85页

作者：买买提江.依米提艾买提江.萨伍提郭春云倪玲贵石油天然气精细化工教育部和自治区重点实验室新疆大学化学化工学院新疆乌鲁木齐830046 新疆吐鲁番自然环境试验研究中心新疆吐鲁番838000

以吐鲁番户外暴晒场为聚丙烯(PP)及耐候性PP的自然老化试验点,连续暴晒12个月。在室外暴晒过程中,研究了材料的断裂伸长率和冲击强度、表面微观形态和元素组成、相对分子质量及其分布的变化,分析了防老化助剂对聚丙烯耐老化性能的影响... 详细信息

以吐鲁番户外暴晒场为聚丙烯(PP)及耐候性PP的自然老化试验点,连续暴晒12个月。在室外暴晒过程中,研究了材料的断裂伸长率和冲击强度、表面微观形态和元素组成、相对分子质量及其分布的变化,分析了防老化助剂对聚丙烯耐老化性能的影响。结果表明,试样PP-0暴晒90 d后断裂伸长率和冲击强度保持率分别降至6.4%和5.6%,氧元素增加了23.21%,重均相对分子质量从24.2万降至8.07万,表面出现大量宽度为3.05μm的裂纹。而耐候改性的PP-3试样中防老化助剂能够起到协同后补作用,抑制热氧光对PP的降解老化速度,从而提高聚丙烯的使用寿命。

关键词：聚丙烯自然老化分子量断裂伸长率

Q235和Q450钢在吐鲁番干热大气环境中长周期暴晒时的腐蚀行为研究

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中国腐蚀与防护学报 2018年第5期38卷 431-437页

作者：王力郭春云肖葵吐尔逊.斯拉依丁董超芳李晓刚北京科技大学腐蚀与防护中心北京100083 新疆吐鲁番自然环境试验研究中心吐鲁番838200

在吐鲁番干热大气环境中对Q235和Q450钢进行4 a大气暴晒实验。结果表明,两种钢表面均有较为明显的锈层,Q450耐候钢4 a的平均腐蚀速率为12 g·m^(-2)·a^(-1),Q235钢平均腐蚀速率为14 g·m^(-2)·a^(-1),Q450钢腐蚀速率相对较低,腐蚀坑... 详细信息

在吐鲁番干热大气环境中对Q235和Q450钢进行4 a大气暴晒实验。结果表明,两种钢表面均有较为明显的锈层,Q450耐候钢4 a的平均腐蚀速率为12 g·m^(-2)·a^(-1),Q235钢平均腐蚀速率为14 g·m^(-2)·a^(-1),Q450钢腐蚀速率相对较低,腐蚀坑深度较浅。腐蚀产物主要由α-FeOOH,γ-FeOOH和Fe_2O_3·H_2O组成,其中Q450钢腐蚀产物中α-FeOOH比例相对较高,腐蚀产物致密。电化学阻抗测试结果表明:Q450钢腐蚀产物电阻远大于Q235钢的,表面电荷转移电阻也大于Q235钢的,即Q450钢耐蚀性较好,腐蚀产物对基体保护作用相对较好。

关键词：碳钢吐鲁番大气腐蚀