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中国循环杂志 2016年第Z1期31卷 -页

作者： Chuanwei Li Gengze Wu chunyu zeng Daping Hospital The Third Military Medical University Chongqing China

Transient induction of actin cytoskeletal remodeling associated with dedifferentiation,proliferation,and redifferentiation stimulates cardiac regeneration

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Acta Pharmaceutica Sinica B 2024年第6期14卷 2537-2553页

作者： Wenbin Fu Qiao Liao Yu Shi Wujian Liu Hongmei Ren Chunmei Xu chunyu zeng Department of Cardiology Daping Hospitalthe Third Military Medical University(Army Medical University)Chongqing 400042China Key Laboratory of Geriatric Cardiovascular and Cerebrovascular Disease Research Ministry of Education of ChinaChongqing 400042China Chongqing Key Laboratory for Hypertension Research Chongqing Cardiovascular Clinical Research CenterChongqing Institute of CardiologyChongqing 400042China State Key Laboratory of Trauma Burns and Combined InjuryDaping Hospitalthe Third Military Medical UniversityChongqing 400042China Cardiovascular Research Center Chongqing CollegeUniversity of Chinese Academy of SciencesChongqing 400042China State Key Laboratory of Trauma and Chemical Poisoning Chongqing 400042China

The formation of new and functional cardiomyocytes requires a 3-step process:dedifferentiation,proliferation,and redifferentiation,but the critical genes required for efficient dedifferentiation,proliferation,and redifferentiation remain *** our study,a circular trajectory using single-nucleus RNA sequencing of the pericentriolar material 1 positive(PCM1^(+))cardiomyocyte nuclei from hearts 1 and 3 days after surgery-induced myocardial infarction(MI)on postnatal Day 1 was reconstructed and demonstrated that actin remodeling contributed to the dedifferentiation,proliferation,and redifferentiation of cardiomyocytes after *** identified four top actin-remodeling regulators,namely Tmsb4x,Tmsb10,Dmd,and Ctnna3,which we collectively referred to as *** expressed changes of 2D2P,using a polycistronic non-integrating lentivirus driven by Tnnt2(cardiac-specific troponin T)promoters(Tnnt2-2D2P-NIL),efficiently induced transiently proliferative activation and actin remodeling in postnatal Day 7 cardiomyocytes and adult ***,the intramyocardial delivery of Tnnt2-2D2P-NIL resulted in a sustained improvement in cardiac function without ventricular dilatation,thickened septum,or fatal arrhythmia for at least 4 *** conclusion,this study highlights the importance of actin remodeling in cardiac regeneration and provides a foundation for new gene-cocktail-therapy approaches to improve cardiac repair and treat heart failure using a novel transient and cardiomyocyte-specific viral construct.

关键词： Single cell analysis Actin remodeling Tmsb4x Tmsb10 Dmd Ctnna3 Myocardial infarction Cardiomyocytes proliferation Cardiac regeneration Genetic therapy

The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway

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Acta Pharmaceutica Sinica B 2022年第5期12卷 2315-2329页

作者： Weimin Yu Gang Xu Hui Chen Li Xiao Gang Liu Pingping Hu Siqi Li Vivi Kasim chunyu zeng Xiaoyong Tong School of Pharmaceutical Sciences Chongqing UniversityChongqing 401331China Institute of Health Biological Chemical Medication Chongqing Institute of Green and Intelligent TechnologyChinese Academy of SciencesChongqing 400714China Institute of Medicine and Equipment for High Altitude Region College of High Altitude Military MedicineArmy Medical University(Third Military Medical University)Chongqing 400038China Key Laboratory of High Altitude Medicine People’s Liberation ArmyChongqing 400038China Henan Key Laboratory of Medical Tissue Regeneration College of Basic Medical SciencesXinxiang Medical UniversityXinxiang 453003China Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing 400044China Department of Cardiology Daping HospitalArmy Medical University(Third Military Medical University)Chongqing 400042China

Pulmonary hypertension(PH) is a life-threatening disease characterized by pulmonary vascular remodeling, in which hyperproliferation of pulmonary artery smooth muscle cells(PASMCs)plays an important role. The cysteine 674(C674) in the sarcoplasmic/endoplasmic reticulum Ca^(2+)ATPase 2(SERCA2) is the critical redox regulatory cysteine to regulate SERCA2 activity. Heterozygous SERCA2 C674 S knock-in mice(SKI), where one copy of C674 was substituted by serine to represent partial C674 oxidative inactivation, developed significant pulmonary vascular remodeling resembling human PH, and their right ventricular systolic pressure modestly increased with age. In PASMCs, substitution of C674 activated inositol requiring enzyme 1 alpha(IRE1 a) and spliced X-box binding protein 1(XBP1 s) pathway, accelerated cell cycle and cell proliferation, which reversed by IRE1 a/XBP1 s pathway inhibitor 4μ8 C. In addition, suppressing the IRE1 a/XBP1 s pathway prevented pulmonary vascular remodeling caused by substitution of C674. Similar to SERCA2 a, SERCA2 b is also important to restrict the proliferation of PASMCs. Our study articulates the causal effect of C674 oxidative inactivation on the development of pulmonary vascular remodeling and PH, emphasizing the importance of C674 in restricting PASMC proliferation to maintain pulmonary vascular homeostasis. Moreover, the IRE1 a/XBP1 s pathway and SERCA2 might be potential targets for PH therapy.

关键词： Pulmonary hypertension Sarcoplasmic/endoplasmic reticulum Ca^(2+)ATPase Pulmonary vascular remodeling Pulmonary artery smooth muscle cell Endoplasmic reticulum stress Oxidative stress

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A long noncoding RNA, CoroMarker, regulates CAD by targeting miR-205-5P

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中国循环杂志 2016年第Z1期31卷 -页

作者： Yue Cai chunyu zeng Department of Cardiology Daping Hospital The Third Military Medical University Chongqing China

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无形财产权体系下传统手工艺权的构建

财经理论与实践 2023年第5期44卷 146-152页

作者：曾春宇中南大学法学院湖南长沙410017

现有知识产权保护模式对传统手工艺的保护囿于三个方面,一是独创性判断标准适用困难以及新颖性要求的法定标准过高,二是传统手工艺商标权概念的不成立,三是文化遗产保护模式中民事法律规范的缺位。在无形财产权体系下传统手工艺可建构... 详细信息

现有知识产权保护模式对传统手工艺的保护囿于三个方面,一是独创性判断标准适用困难以及新颖性要求的法定标准过高,二是传统手工艺商标权概念的不成立,三是文化遗产保护模式中民事法律规范的缺位。在无形财产权体系下传统手工艺可建构一项独立的财产权。在此基础上选择特别权利保护模式,可避开与传统产权保护模式的要件冲突。厘定传统手工艺权的主体客体、权利授权方式、权利内容、权利限制与保护期限,构成传统手工艺特别权利保护制度,将更有利于传统手工艺的传承保护与创新发展。

关键词：传统手工艺权无形财产权特别权利保护模式

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Circulating 'lncRNA OTTHUMT00000387022' from monocytes as a novel biomarker for coronary artery disease

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Circulating 'lncRNA OTTHUMT00000387022' from monocytes as a ...

Sinoatrial node pacemaker cells share dominant biological properties with glutamatergic neurons

第十一届海峡两岸心血管科学研讨会、2017年全国心脑血管药理学术会议、中国卒中学会第一次新药研讨会

作者： cai yue zeng chunyu Zhou Lin Department of Cardiology Daping HospitalThe Third Military Medical University Chongqing Institute of Cardiology

Aims Long non-coding RNAs(lncRNAS) have been found to be involved in the pathogenesis of coronary artery disease(CAD).However,it remains to be established whether or not circulating lncRNAs can serve as biomarkers of *** and results Using a microarray-based lncRNA expression profiling,we found 86 lncRNAs that were differentially expressed in circulating peripheral blood monocytes and plasma from 15 CAD patients and 15 control *** choosing a consistent criterion(average normalized intensity ≥7

关键词： lncRNA OTTHUMT00000387022 Circulating from monocytes as a novel biomarker for coronary artery disease

来源： cnki会议评论

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Protein & Cell 2021年第7期12卷 545-556页

作者： Dandan Liang Zhigang Xue Jinfeng Xue Duanyang Xie Ke Xiong Huixing Zhou Fulei Zhang Xuling Su Guanghua Wang Qicheng Zou Yi Liu Jian Yang Honghui Ma Luying Peng chunyu zeng Gang Li Li Wang Yi-Han Chen Department of Cardiology East HospitalTongji University School of MedicineShanghai 200120China Key Laboratory of Arrhythmias of the Ministry of Education of China Tongji University School of MedicineShanghai 200120China Institute of Medical Genetics Tongji UniversityShanghai 200092China Department of Regenerative Medicine Tongji University School of MedicineShanghai 200092China Reproductive Medicine Center Tongji HospitalTongji University School of MedicineShanghai 200065China Department of Pathology and Pathophysiology Tongji University School of MedicineShanghai 200092China Department of Cardiology Daping HospitalChongqing 400042China Department of Neurology East HospitalTongji University School of MedicineShanghai 200120China State Key Laboratory of Cardiovascular Disease Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China

Activation of the heart normally begins in the sinoatrial node(SAN).Electrical impulses spontaneously released by SAN pacemaker cells(SANPCs)trigger the contraction of the ***,the cellular nature of SANPCs remains ***,we report that SANPCs exhibit glutamatergic neuron-like *** comparing the single-cell transcriptome of SANPCs with that of cells from primary visual cortex in mouse,we found that SANPCs co-clustered with cortical *** and cellular imaging confirmed that SANPCs contained key elements of glutamatergic neurotransmitter system,expressing genes encoding glutamate synthesis pathway(G/s),ionotropic and metabotropic glutamate receptors(Grina,Gria3,Grm1 and Grm5)t and glutamate transporters(Slc17a7).SANPCs highly expressed cell markers of glutamatergic neurons(Snap25 and S/-c17a7)t whereas Gad1,a marker of GABAergic neurons,was *** studies revealed that inhibition of glutamate receptors or transporters reduced spontaneous pacing frequency of isolated SAN tissues and spontaneous Ca2+transients frequency in single ***,our work suggests that SANPCs share dominant biological properties with glutamatergic neurons,and the glutamatergic neurotransmitter system may act as an intrinsic regulation module of heart rhythm,which provides a potential intervention target for pacemaker cell-associated arrhythmias.

关键词： sinoatrial node pacemaker cell glutamatergic neuron single-cell RNA-seq electrophysiology

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Cardiac troponinⅠexacerbates myocardial ischemia/reperfusion injury by inducing the adhesion of monocytes to vascular endothelial cells via a TLR4/NF-κB-dependent pathway

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中国循环杂志 2016年第Z1期31卷 -页

作者： Yu Han Xiang Liao Zhao Gao Sufei Yang Caiyu Chen Yukai Liu Wei Eric Wang Gengze Wu Xiongwen Chen Pedro A Jose Ye Zhang chunyu zeng Department of Cardiology Daping Hospital The Third Military Medical University Chongqing China Department of Medicine Division of RenalDisease and Hypertension and Department of Physiology The George Washington University Schoolof Medicine&Health Sciences Washington D. C. USA

Discovering small molecules as Wnt inhibitors that promote heart regeneration and injury repair

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Journal of Molecular Cell Biology 2020年第1期12卷 42-54页

作者： Shuying Xie Wenbin Fu Guangju Yu Xueli Hu Kaa Seng Lai Xiangwen Peng Yating Zhou Xuejiao Zhu Ptamen Christov Leah Sawyer Terri T.Ni Gary A.Sulikowski Zhongzhou Yang Ethan Lee chunyu zeng Wei E.Wang Tao P.Zhong State Key Laboratory of Genetic Engineering School of Life SciencesZhongshan HospitalFudan UniversityShanghai 200438China Department of Cardiology Daping HospitalThird Military Medical UniversityChongqing 400042China Shanghai Key Laboratory of Regulatory Biology Institute of Molecular MedicineEast China Normal University School of Life SciencesShanghai 200241China Vanderbilt Institute of Chemical Biology Vanderbilt UniversityNashvilleTN 37232USA Department of Cell and Developmental Biology Vanderbilt UniversityNashvilleTN 37232USA MOE Key Laboratory of Model Animal for Disease Study Model Animal Research CenterNanjing UniversityNanjingChina

There are intense interests in discovering pro regenerative medicine leads that can promote cardiac differentiation and regeneration,as well as repair damaged heart *** have combined zebrafish embryo-based screens with cardiomyogenesis assays to discover selective small molecules that modulate heart development and regeneration with minimal adverse *** related compounds with novel structures,named as Cardiomogen 1 and 2(CDMG1 and CDMG2),were identified for their capacity to promote myocardial hyperplasia through expansion of the cardiac progenitor cell *** find that Cardiomogen acts as a Wnt inhibitor by targeting p-catenin and reducing Tcf/Lef-mediated transcription in cultured *** treatment of amputated zebrafish hearts reduces nuclear p-catenin in injured heart tissue,increases cardiomyocyte(CM)proliferation,and expedites wound healing,thus accelerating cardiac muscle ***,Cardiomogen can alleviate the functional deterioration of mammalian hearts after myocardial *** hearts exposed to CDMG1 display increased newly formed CMs and reduced fibrotic scar tissue,which are in part attributable to the^-catenin *** findings indicate Cardiomogen as a Wnt inhibitor in enhancing injury-induced CM proliferation and heart regeneration,highlighting the values of embryo-based small molecule screens in discovery of effective and safe medicine leads.

关键词： small molecule screen Wnt inhibitor regeneration

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单细胞组学视角下的心肌细胞增殖

陆军军医大学学报 2022年第1期44卷 69-73页

作者：曾春雨付文斌陆军特色医学中心心血管内科重庆市心血管病研究所

单细胞多组学测序技术是在单细胞分辨率下精确分析细胞异质性和基因调控网络的新技术,提供了全新的视角来分析细胞的分化路径、细胞间的交互调控和细胞亚群的分离现象。心肌再生与修复是逆转心肌损伤后重构的重要研究方向。目前,单细胞... 详细信息

单细胞多组学测序技术是在单细胞分辨率下精确分析细胞异质性和基因调控网络的新技术,提供了全新的视角来分析细胞的分化路径、细胞间的交互调控和细胞亚群的分离现象。心肌再生与修复是逆转心肌损伤后重构的重要研究方向。目前,单细胞多组学测序技术已应用于心肌再生领域:构建生理和病理条件下心肌再生图谱,探索心肌细胞增殖的分化路径和非心肌细胞对心肌再生的交互作用,建立全新的连续细胞周期分子标志监测的心肌细胞增殖评价体系以及寻找调控心肌细胞增殖的分子开关等,具有广阔的前景。本述评总结了近几年心肌再生领域的最新研究和进展,探讨单细胞技术在心肌再生领域研究中的应用及今后研究的方向,以期为后续研究提供积极合理的参考。

关键词：单细胞多组学测序心肌再生心肌细胞增殖