检索结果-南通市图书馆

Jigsaw puzzle of SARS-CoV-2 RBD evolution and immune escape

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2022年第7期19卷 848-851页

作者： chunyan yi Xiaoyu Sun Zhiyang Ling Bing Sun State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech University100 Haike RoadShanghai 201210China

The outbreak of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which causes coronavirus disease 2019(COVID-19),has posed a tremendous threat to global health,resulting in over 510 million infections and at least 6.23 million deaths worldwide as of early May *** efforts to fight against the SARS-CoV-2 pandemic have accelerated the research and clinical development of various vaccines and neutralizing antibodies(NAbs)[1,2].However,SARS-CoV-2 has an exceptional ability to mutate and evade the human immune system,which highlights the surveillance of SARS-CoV-2 evolution to anticipate and facilitate a timely response to new variants that could impact viral transmissibility and the efficacy of vaccines and antibody therapies[3].

关键词： acute respiratory evolution

Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2020年第6期17卷 621-630页

作者： chunyan yi Xiaoyu Sun Jing Ye Longfei Ding Meiqin Liu Zhuo Yang Xiao Lu Yaguang Zhang Liyang Ma Wangpeng Gu Aidong Qu Jianqing Xu Zhengli Shi Zhiyang Ling Bing Sun State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of Sciences200031 ShanghaiChina School of Life Science and Technology ShanghaiTech University201210 ShanghaiChina Shanghai Public Health Clinical Center Fudan University201508 ShanghaiChina Key Laboratory of Special Pathogens Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of Sciences430071 WuhanChina Shanghai Institute of Biological Products 200052 ShanghaiChina

Coronavirus disease 2019(COVID-19),caused by the novel human coronavirus SARS-CoV-2,is currently a major threat to public health *** viral spike protein binds the host receptor angiotensin-converting enzyme 2(ACE2)via the receptor-binding domain(RBD),and thus is believed to be a major target to block viral *** SARS-CoV-2 and SARS-CoV share this *** we functionally analyzed the key amino acid residues located within receptor binding motif of RBD that may interact with human ACE2 and available neutralizing *** in vivo experiments showed that immunization with either the SARS-CoV RBD or SARS-CoV-2 RBD was able to induce strong clade-specific neutralizing antibodies in mice;however,the cross-neutralizing activity was much weaker,indicating that there are distinct antigenic features in the RBDs of the two *** finding was confirmed with the available neutralizing monoclonal antibodies against SARS-CoV or *** is worth noting that a newly developed SARS-CoV-2 human antibody,HA001,was able to neutralize SARS-CoV-2,but failed to recognize ***,the potential epitope residues of HA001 were identified as A475 and F486 in the SARS-CoV-2 RBD,representing new binding sites for neutralizing ***,our study has revealed the presence of different key epitopes between SARS-CoV and SARSCoV-2,which indicates the necessity to develop new prophylactic vaccine and antibody drugs for specific control of the COVID-19 pandemic although the available agents obtained from the SARS-CoV study are unneglectable.

关键词： SARS-CoV-2 spike protein receptor binding motif cross-neutralizing antibody substitution mutation

A SARS-CoV-2 antibody retains potent neutralization against Omicron by targeting conserved RBM residues

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2022年第5期19卷 647-649页

作者： chunyan yi Zhiyang Ling Xiao Lu Yadong Fu Zhuo Yang Sonam Wangmo Shuangfeng Chen Yaguang Zhang Liyan Ma Wangpeng Gu Hongzhou Lu Xiaoyu Sun Bing Sun State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai200031China School of Life Science and Technology ShanghaiTech UniversityShanghai201210China National Clinical Center for Infectious Disease The Third People’s Hospital of Shenzhen and the Second Affiliated Hospital of Southern University of Science and TechnologyShenzhen518112Guangdong ProvinceChina Shanghai Public Health Clinical Center Shanghai Medical CollegeFudan UniversityShanghai201508China

The newly emerged Omicron(B.1.1.529)variant of SARS-CoV-2 is quickly overtaking the Delta variant and becoming the dominant strain around the world due to its enhanced transmissibility and high immune escape potential[1,2].Compared to the Wuhan strain,the Omicron variant carries 37 spike mutations,of which 15 are within the receptor-binding domain(RBD)(Fig.1A).A high mutation rate is associated with remarkable resistance to current vaccines and RBD-specific antibody therapeutics[2,3,4].

关键词： residues becoming Omi

Potential contribution of increased soluble IL-2R to lymphopenia in COVID-19 patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2020年第8期17卷 878-880页

作者： Yaguang Zhang Xiaojing Wang Xuezhen Li Dong Xi Ruizhi Mao Xiaohui Wu Shipeng Cheng Xiaoyu Sun chunyan yi Zhiyang Ling Liyan Ma Qin Ning yiru Fang Bing Sun Di Wu State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of Sciences320 Yueyang Road200031ShanghaiChina Department and Institute of Infectious Disease Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology430030WuhanChina Clinical Research Center and Division of Mood Disorders Shanghai Mental Health CenterShanghai Jiao Tong University School of Medicine200030ShanghaiChina

Since the outbreak of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),more than 6 million cases are confirmed and over 300,000 cases are dead after *** of immunity in COVID-19 patients has been considered as one of the fatal factors for patients,especially cytokine release syndrome and lymphopenia.1,2,3 The reduced number and increased exhaustion level of lymphocyte are associated with elevated inflammatory cytokines in COVID-19 patients.4,5 However,the mechanism of cytokine-induced lymphopenia in COVID-19 is very ***-2 is critical for the proliferation,differentiation,and function of T cells,including Tregs,CD4+,and CD8+effector cells.6 Here,we reported the negative relationship between the concentration of soluble IL-2 receptor(sIL-2R)and T-cell number in blood from COVID-19 *** vitro addition of recombinant CD25 could inhibit the proliferation and function of T cells from PBMC after stimulated with TCR signaling,which could be rescued by strong IL-2 *** data suggested the importance of IL-2 signaling in lymphopenia of COVID-19 patients.

关键词： patients lympho immunity

A human antibody potently neutralizes RSV by targeting the conserved hydrophobic region of prefusion F

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2023年第4期66卷 729-742页

作者： chunyan yi Caixia Su Xiaoyu Sun Xiao Lu Chuanya Si Caixuan Liu Zhuo Yang Hong Yuan Yuying Huang Jing Wen Yonghui He Yaguang Zhang Liyan Ma Yao Cong Gan Zhao Zhiyang Ling Bin Wang Bing Sun State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Key Laboratory of Medical Molecular Virology(MOE/MOH) Department of Medical Microbiology and ParasitologySchool of Basic Medical SciencesShanghai Medical CollegeFudan UniversityShanghai 200032China MedimScience.Co Hangzhou 311217China Shanghai Institute of Infectious Disease and Biosecurity Shanghai Medical CollegeFudan UniversityShanghai 200032China

Respiratory syncytial virus(RSV) continues to pose serious threats to pediatric populations due to the lack of a vaccine and effective antiviral drugs. RSV fusion(F) glycoprotein mediates viral-host membrane fusion and is a key target for neutralizing antibodies. We generated 23 full-human monoclonal antibodies(hm Abs) against prefusion F protein(pre-F) from a healthy adult with natural RSV infection by single B cell cloning technique. A highly potent RSV-neutralizing hm Ab, named as 25-20, is selected, which targets a new site Φ-specific epitope. Site-directed mutagenesis and structural modelling analysis demonstrated that 25-20 mainly targets a highly conserved hydrophobic region located at the a4 helix and a1 helix of pre-F, indicating a site of vulnerability for drug and vaccine design. It is worth noting that 25-20 uses an unreported inferred germline(i GL) that binds very poorly to pre-F, thus high levels of somatic mutations are needed to gain high binding affinity with pre-F. Our observation helps to understand the evolution of RSV antibody during natural infection. Furthermore, by in silico prediction and experimental verification, we optimized 25-20 with KD values as low as picomolar range. Therefore, the optimized 25-20 represents an excellent candidate for passive protection against RSV infection.

关键词： RSV human antibody potent neutralization conserved epitope somatic mutations affinity maturation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

男式针织毛衫的创新设计

毛纺科技 2017年第5期45卷 23-27页

作者：马春艳周颐东北农业大学艺术学院黑龙江哈尔滨150030

分析了国内外男式针织毛衫设计现状,认为目前国内市场中男式针织毛衫款式依然偏于传统和陈旧,提出男式针织毛衫的开发、创新已成为针织时尚界一个迫于解决的问题。分别从男式毛衫款式廓形与局部设计、男式毛衫针型与纱线选择、男式毛衫... 详细信息

分析了国内外男式针织毛衫设计现状,认为目前国内市场中男式针织毛衫款式依然偏于传统和陈旧,提出男式针织毛衫的开发、创新已成为针织时尚界一个迫于解决的问题。分别从男式毛衫款式廓形与局部设计、男式毛衫针型与纱线选择、男式毛衫组织结构与色彩花型设计等方面进行分析,指出传统男式针织毛衫的特点与局限,并以理论与举例相结合的方式提出产品设计的创新角度,为企业与设计师开发男式毛衫新产品提供有效思路。提出设计师应与时俱进,开拓创新思维,多角度展现男式针织毛衫的内涵、个性与魅力,以适应瞬息万变的市场竞争。

关键词：男式针织毛衫设计创新

Junctional and somatic hypermutation-induced CX4C motif is critical for the recognition of a highly conserved epitope on HCV E2 by a human broadly neutralizing antibody

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2021年第3期18卷 675-685页

作者： chunyan yi Jing Xia Lan He Zhiyang Ling Xuesong Wang Yu Yan Jiangjun Wang Xinhao Zhao Weiguo Fan Xiaoyu Sun Ronghua Zhang Sheng Ye Rongguang Zhang Yongfen Xu Liyan Ma Yaguang Zhang Honglin Zhou Zhong Huang Junqi Niu Gang Long Junxia Lu Jin Zhong Bing Sun State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of Sciences/University of Chinese Academy of SciencesShanghaiChina CAS Key Laboratory of Molecular Virology and Immunology Institut Pasteur of ShanghaiChinese Academy of Sciences/University of Chinese Academy of SciencesShanghaiChina School of Life Science and Technology ShanghaiTech UniversityShanghaiChina CAS Key Laboratory of Synthetic Chemistry of Natural Substances Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghaiChina College of Biology Hunan Provincial Key Laboratory of Medical VirologyHunan UniversityChangshaChina National Laboratory of Biophysics Institute of BiophysicsChinese Academy of Sciences/University of Chinese Academy of Sciences BdjingBeijingChina Interdisciplinary Innovation Institute of Medicine&Engineering Beijing Advanced Innovation Center for Big Data-Based Precision MedicineSchool of Biological Science and Medical EngineeringBeihang UniversityBeijingChina Nanjing Galaxy Biopharma Co. LtdNanjingChina Hepatology Section First HospitalUniversity of JilinChangchunChina

Induction of broadly neutralizing monoclonal antibodies(bNAbs)that bind to the viral envelope glycoproteins is a major goal of hepatitis C virus(HCV)vaccine *** study of bNAbs arising in natural infection is essential in this *** generated a human antibody,8D6,recognizing the E2 protein of HCV isolated from a chronic hepatitis C *** antibody shows broadly neutralizing activity,which covers a pan-genotypic panel of cell culture-derived HCV virions(HCVcc).Functional and epitope analyses demonstrated that 8D6 can block the interaction between E2 and CD81 by targeting a highly conserved epitope on *** describe how the 8D6 lineage evolved via somatic hypermutation to achieve broad *** found that the V(D)J recombination-generated junctional and somatic hypermutation-induced disulfide bridge(C-C)motif in the CDRH3 is critical for the broad neutralization and binding activity of *** motif is conserved among a series of broadly neutralizing HCV antibodies,indicating a common binding ***,the 8D6 inferred germline(iGL)was reconstructed and tested for its binding affinity and neutralization ***,8D6 iGL-mediated relatively strong inhibition of the 1b genotype PR79L9 strain,suggesting that PR79L9 may serve as a potential natural viral strain that provides E2 sequences that induce ***,our detailed epitope mapping and genetic studies of the HCV E2-specific mAb 8D6 have allowed for further refinement of antigenic sites on E2 and reveal a new mechanism to generate a functional CDRH3,while its iGL can serve as a probe to identify potential HCV vaccine strains.

关键词： Hepatitis C virus neutralizing antibody inferred germline CDRH3 disulfide bridge motif

Vitamin B6 regulates IL-33 homeostasis to alleviate type 2 inflammation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2023年第7期20卷 794-807页

作者： Songling Zhu Shufen Zhong Kebin Cheng Li-Sha Zhang Jiu-wu Bai Zu Cao Su Wang Wen Chen Shipeng Cheng Liyan Ma Zhiyang Ling Yuying Huang Wangpeng Gu Xiaoyu Sun chunyan yi Meng Zhao Shuo Liang Jin-Fu Xu Bing Sun Yaguang Zhang Division of Life Sciences and Medicine University of Science and Technology of ChinaHefei 230022China State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of Sciences320 Yueyang RoadShanghai 200031China Department of Respiratory and Critical Care Medicine Shanghai Pulmonary HospitalInstitute of Respiratory MedicineSchool of MedicineTongji University507 Zhengmin RoadShanghai 200433China

Interleukin-33(IL-33)is a crucial nuclear cytokine that induces the type 2 immune response and maintains immune *** fine-tuned regulation of IL-33 in tissue cells is critical to control of the type 2 immune response in airway inflammation,but the mechanism is still ***,we found that healthy individuals had higher phosphate-pyridoxal(PLP,an active form of vitamin B6)concentrations in the serum than asthma *** serum PLP concentrations in asthma patients were strongly associated with worse lung function and *** a mouse model of lung inflammation,we revealed that PLP alleviated the type 2 immune response and that this inhibitory effect relied on the activity of IL-33.A mechanistic study showed that in vivo,pyridoxal(PL)needed to be converted into PLP,which inhibited the type 2 response by regulating IL-33 *** mice heterozygous for pyridoxal kinase(PDXK),the conversion of PL to PLP was limited,and IL-33 levels were increased in the lungs,aggravating type 2 ***,we found that the mouse double minute 2 homolog(MDM2)protein,an E3 ubiquitin-protein ligase,could ubiquitinate the N-terminus of IL-33 and sustain IL-33 stability in epithelial *** reduced MDM2-mediated IL-33 polyubiquitination and decreased the level of IL-33 through the proteasome *** addition,inhalation of PLP alleviated asthma-related effects in mouse *** summary,our data indicate that vitamin B6 regulates MDM2-mediated IL-33 stability to constrain the type 2 response,which might help develop a potential preventive and therapeutic agent for allergy-related diseases.

关键词： Type 2 inflammation IL-33 Vitamin B6

Protective humoral immunity in SARS-CoV-2 infected pediatric patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2020年第7期17卷 768-770页

作者： Yaguang Zhang Jin Xu Ran Jia chunyan yi Wangpeng Gu Pengcheng Liu Xinran Dong Hao Zhou Bo Shang Shipeng Cheng Xiaoyu Sun Jing Ye Xuezhen Li Jia Zhang Zhiyang Ling Liyan Ma Bingbing Wu Mei Zeng Wenhao Zhou Bing Sun State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of Sciences320 Yueyang RoadShanghai 200031China Children’s Hospital of Fudan University 399 Wanyuan RoadShanghai 201102China Shanghai Kehua Bio-Engineering Co. Ltd.1189 Qinzhou RoadShanghai 200233China

After the rapid spread of SARS-CoV-2 in Wuhan,China,at the beginning of 2020,about 1.5 million confirmed cases and over 80,000 deaths have been reported around 200 countries and territories all over the world and the number continues to ***,we still have limited knowledge of this new coronavirus,especially the interaction between SARS-CoV-2 and our immune system.

关键词： immunity pediatric infected