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Journal of Integrative Plant Biology 2011年第1期53卷 14-24页

作者： chunyan gong Tang Li Qi Li Longfeng Yan Tai Wang Research Center forMolecular &-Deveiopmental Biology Key Laboratory of Photosynthesis & Environmental Molecular Physiology Institute of Botany. the Chinese Academy of Sciences and National Center for Plant Gene Research Beijing 100093 China Graduate School of the Chinese Academy of Sciences. Beiiina 100049 China

Rad21 and its meiotic counterpart Rec8, the key components of the cohesin complex, are essential for sister chromatid cohesion and chromosome segregation in mitosis and meiosis, respectively. In contrast to yeast and vertebrates, which have only two RAD211REC8 genes, the rice genome encodes four Rad21/Rec8 proteins. Here, we report on the cloning and characterization of OsRAD21-2 from rice （Oryza sativa L.）. Phylogenetic analysis of the full-length amino acids showed that OsRad21-2 was grouped into the plant-specific Rad21 subfamily. Semi-quantitative reverse transcription-polymerase chain reaction revealed OsRAD21-2 preferentially expressed in premeiotic flowers. Further RNA in situ hybridization analysis and promoter：：β-glucuronidase staining indicated that OsRAD21-2 was mainly expressed in actively dividing tissues including premeiotic stamen, stem intercalary meristem, leaf meristem, and root pericycle. Ectopic expression of OsRAD21.2 in fission yeast resulted in cell growth delay and morphological abnormality. Flow cytometric analysis revealed that the *** cells were arrested in G2 phase. Our results suggest that OsRad21-2 functions in regulation of cell division and growth.

关键词： RICE PLANT gene expression PLANT chromosomes CELL division (Biology) CELLULAR growth PLANT genomes GENETIC code

Preparation and identification of monoclonal antibodies against the adenovirus vector

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The Chinese-German Journal of Clinical Oncology 2007年第4期6卷 411-414页

作者： chunyan Zhang Jinsong gong Yan Chen Fanghe Li Experimental Medicine Center Tongji Hospital Tongji Medical College Huazhong University of Science andTechnology Wuhan 430030 China

Objective： To prepare and identity monoclonal antibodies （McAbs） against the capsid proteins of adenovirus vector. Methods： BALB/c mice were immunized with a mixture of the purified adenovirus vector （Adv） and AI（OH）3. McAbs were produced using cell fusion technique in a conventional way. The sensitivity and specificity of monoclonal antibodies was identified by indirect enzyme linked immunosorbent assay （ELISA）, immunocytochemical staining and Western blotting. Results： Six strains of hybridoma cells （A4H11, A8C7, F1H5, G1D2, G4E3 and H2G8） that can stably secrete the IgG1 McAb against Adv were obtained. After 3 months subculture and low concentration of serum adapting culture, six strains retained their stability to secrete McAb. The ascites titers were between 1：10^6 and 1：10^8. Western blot analysis demonstrated that all the McAbs reacted with one protein （about 114 kDa） which is present in wild type 3 adenovirus （wtAd3）, wild type 5 adenovirus （wtAd5）, wild type 7 adenovirus （wtAd7） and adenovirus vector. Conclusion： Successfully prepared six strains of hybridoma cell secreted monoclonal antibodies against the hexon proteins of adenovirus vector, and provided the substantial foundation of preclinical research of adenovirus vectors.

关键词： gene therapy adenovirus adenovirus vector monoclonal antibodies

Radix Polygoni Multiflori Praeparata and Dioscorea Bulbifera Rhizome Decoctions Display Combined Effects Detected by a Three-Probe Drug Cocktail with Substrates of Rat Hepatic Cytochrome P450 Enzymes

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Pharmacology & Pharmacy 2014年第7期5卷 736-746页

作者： Li Jiang Pingping Shan Hui Yu Jiayuan Tao chunyan gong Guoqing Shen Institute of Dermatology Chinese Academy of Medical Sciences and Peking Union Medical College Nanjing China

Objectives: Radix Polygoni Multiflori Praeparata (RPMP) and Dioscorea Bulbifera Rhizomes (DBR) are used in Chinese herbal medicine and have been frequently reported for adverse reactions on liver. In this research, we aimed to evaluate in vivo effects of RPMP and DBR on rat cytochrome P450 enzymes (CYP1A2, CYP2E1 and CYP3A2) with their respective substrates as probes. Methods: Rats were orally administered RPMP, DBR and RPMP/DBR combination at 12, 10 and (12 + 10) g/kg, respectively, or saline as a control, once daily for 7 days. Thereafter, a cocktail containing 10 mg/kg caffeine, 20 mg/kg chlorzoxazone and 10 mg/kg dapsone was tail vein injected to rats. At defined time points, plasma drug concentrations were simultaneously evaluated by HPLC. Pharmacokinetic parameters simulated by DAS software were used to assess RPMP and/or DBR effects on cytochrome P450 enzymes activity. ANOVA and Dunnett’s test were used for data analysis. Results: Caffeine metabolism was enhanced in RPMP animals and reduced after pretreatment with DBR, but no effect was observed in RPMP/DBR combination group. Chlorzoxazone and dapsone metabolism was enhanced in both RPMP and DBR groups and consequently in combination group. The data suggested that RPMP independently induces rat CYP1A2, CYP2E1 and CYP3A2 activity, while DBR independently inhibits activity of rat CYP1A2 and induces that of CYP2E1 and CYP3A2. RPMP/DBR combination showed no significant benefit compared with the two drugs alone and even showed a neutralized effect in CYP1A2 activity. Conclusions: Caution is needed when RPMP and/or DBR are co-administered with drugs metabolized by human CYP1A2, CYP2E1 and CYP3A4.

关键词： Radix Polygoni Multiflori Praeparata Dioscorea Bulbifera Rhizomes Cytochrome P450 Herb-Drug Interactions Three-Probe Drug Cocktail

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JAK抑制剂在难治性皮肌炎中的应用进展

中华皮肤科杂志 2021年第3期54卷 264-267页

作者：花玮玮龚春燕中国医学科学院、北京协和医学院皮肤病医院药剂科南京210042

JAK/STAT信号通路在皮肌炎发病中起着重要作用。JAK抑制剂能阻断依赖JAK/STAT通路相关因子的信号转导,抑制免疫细胞激活和T细胞介导的炎症性反应。目前美国食品药品监督管理局批准的JAK抑制剂适应证只有风湿性关节炎和骨髓纤维化,但已... 详细信息

JAK/STAT信号通路在皮肌炎发病中起着重要作用。JAK抑制剂能阻断依赖JAK/STAT通路相关因子的信号转导,抑制免疫细胞激活和T细胞介导的炎症性反应。目前美国食品药品监督管理局批准的JAK抑制剂适应证只有风湿性关节炎和骨髓纤维化,但已有不少应用JAK抑制剂治疗难治性皮肌炎的报道。本文综述JAK/STAT通路在皮肌炎发病机制中的作用、JAK抑制剂治疗皮肌炎的作用机制及临床应用。

关键词：皮肌炎 Janus激酶类 STAT转录因子类干扰素Ⅰ型 JAK抑制剂托法替尼鲁索利替尼 JAK/STAT信号通路

治疗斑块状银屑病和银屑病关节炎的新药——阿普斯特

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中华皮肤科杂志 2017年第2期50卷 145-148页

作者：孙洁龚春燕中国医学科学院北京协和医学院皮肤病研究所药剂科南京210042

银屑病是一种慢性炎症性皮肤疾病，斑块状银屑病是最常见的类型，约占80％。7％～40％的银屑病患者会发展成关节病性银屑病。传统治疗银屑病的药物包括甲氨蝶呤、环孢素、阿维A等，治疗银屑病关节炎的药物包括甲氨蝶呤、柳氮磺吡啶、来... 详细信息

银屑病是一种慢性炎症性皮肤疾病，斑块状银屑病是最常见的类型，约占80％。7％～40％的银屑病患者会发展成关节病性银屑病。传统治疗银屑病的药物包括甲氨蝶呤、环孢素、阿维A等，治疗银屑病关节炎的药物包括甲氨蝶呤、柳氮磺吡啶、来氟米特等，它们各有不同程度的疗效，但不良反应也多。

关键词：银屑病关节炎斑块状银屑病传统治疗炎症性皮肤疾病关节病性银屑病新药甲氨蝶呤银屑病患者

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复方熊果苷乳膏致接触性皮炎

药物不良反应杂志 2021年第2期23卷 110-111页

作者：朱超龚春燕中国医学科学院皮肤病医院药剂科南京210042

1例26岁女性因面部雀斑行激光脱色治疗,术后予复方熊果苷乳膏(1 g、每晚1次)面部外敷。首次敷用该药约12 h后面部出现红肿和小水疱疹伴脱屑。诊断为接触性皮炎,考虑与复方熊果苷乳膏有关。停用复方熊果苷乳膏,予醋酸泼尼松片20 mg口服、... 详细信息

1例26岁女性因面部雀斑行激光脱色治疗,术后予复方熊果苷乳膏(1 g、每晚1次)面部外敷。首次敷用该药约12 h后面部出现红肿和小水疱疹伴脱屑。诊断为接触性皮炎,考虑与复方熊果苷乳膏有关。停用复方熊果苷乳膏,予醋酸泼尼松片20 mg口服、1次/d,左西替利嗪片5 mg口服、每晚1次,3%硼酸洗液局部湿敷。3 d后患者面部红肿、脱屑明显好转,大部分水疱疹消失;1周后电话随访,水疱疹消失。

关键词：熊果苷皮炎,接触性

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深度学习在结肠息肉分割中的应用综述

计算机工程与应用 2023年第23期59卷 15-27页

作者：孙福艳王琼吕宗旺龚春艳河南工业大学信息科学与工程学院郑州450001 中原智慧园区与智能建筑研究院郑州450001

大部分结直肠癌起源于结肠息肉的恶性病变,使用计算机辅助诊断系统实现结肠息肉的自动精准分割具有重要的临床意义,能够在结肠镜检查过程中辅助医生提高息肉检出率。目前深度学习技术在医学图像分割领域应用广泛,基于深度学习的结肠息... 详细信息

大部分结直肠癌起源于结肠息肉的恶性病变,使用计算机辅助诊断系统实现结肠息肉的自动精准分割具有重要的临床意义,能够在结肠镜检查过程中辅助医生提高息肉检出率。目前深度学习技术在医学图像分割领域应用广泛,基于深度学习的结肠息肉分割算法也取得了重大进展。简要介绍了传统息肉分割算法及其优点和局限性。重点从三个方面对深度学习息肉分割算法进行综述:基于经典CNN结构、基于U-Net结构和基于多模型融合的分割模型,并总结算法改进策略及其优势和局限性。归纳结肠息肉图像公开数据集及数据预处理方法,最后总结基于深度学习的息肉分割研究面临的挑战,并对该领域未来的研究方向做出展望。

关键词：结肠息肉深度学习医学图像分割息肉分割

白介素6在痤疮囊肿皮损中的表达及痤疮丙酸杆菌体外对THP-1细胞白介素6水平的影响

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中华皮肤科杂志 2018年第4期51卷 265-268页

作者： Xu Changchun, Du Leilei, Zeng Rong, Tong Jianbo, Liu Yuzhen, Duan Zhimin, Chen Xu, Xu Haoxiang, gong chunyan, Li Min 中国医学科学院北京协和医学院皮肤病研究所江苏省皮肤性病分子生物学重点实验室南京210042

目的检测寻常痤疮患者囊肿皮损中白介素6（IL-6）的表达，并观察痤疮丙酸杆菌体外对人急性单核细胞白血病细胞系THP-1信号分子p38MAPK及白介素6水平的影响。方法实时荧光定量PCR检测6例痤疮患者囊肿皮损和6例健康人皮肤组织中IL-6 mRN... 详细信息

目的检测寻常痤疮患者囊肿皮损中白介素6（IL-6）的表达，并观察痤疮丙酸杆菌体外对人急性单核细胞白血病细胞系THP-1信号分子p38MAPK及白介素6水平的影响。方法实时荧光定量PCR检测6例痤疮患者囊肿皮损和6例健康人皮肤组织中IL-6 mRNA表达水平。用2 × 106、2 × 107、2 × 108 CFU/ml灭活痤疮丙酸杆菌菌悬液或脂多糖（100 μg/L）刺激THP-1细胞，同时设培养基对照组和p38MAPK抑制剂SB203580组（先用20 μmol/L SB203580处理，再用2 × 108 CFU/ml痤疮丙酸杆菌刺激），作用不同时间（1 ~ 6 h）后，实时荧光定量PCR检测IL-6 mRNA表达水平，酶联免疫吸附试验检测上清液中IL-6含量。2 × 108 CFU/ml灭活痤疮丙酸杆菌菌悬液刺激THP-1细胞15、30、60 min或脂多糖（100 μg/L）刺激30 min后，Western印迹法检测p38MAPK和磷酸化p38MAPK表达水平。结果痤疮囊肿皮损和健康对照皮肤IL-6 mRNA水平分别为3.680 ± 0.790、1.155 ± 0.250，两组比较差异有统计学意义（t = 3.047，P 〈 0.05）。双因素方差分析显示，不同浓度痤疮丙酸杆菌组、脂多糖组、培养基对照组IL-6 mRNA表达水平差异有统计学意义（F = 532.3，P 〈 0.001，ν = 4），痤疮丙酸杆菌刺激1、3、6 h之间差异也有统计学意义（F = 526.6，P 〈 0.001，ν = 2）。2 × 108 CFU/ml痤疮丙酸杆菌组、脂多糖组、培养基对照组上清液中IL-6浓度分别为（1 618.22 ± 32.23）、（3 212.06 ± 353.00）、（147.10 ± 0.53） ng/L，3组间差异有统计学意义（ν = 2，F = 102.35，P 〈 0.01）。2 × 108 CFU/ml痤疮丙酸杆菌作用于THP-1细胞15、30、60 min后磷酸化p38MAPK蛋白水平升高。SB203580组THP-1细胞IL-6 mRNA水平与未抑制组比较明显降低（t = 15.91，P = 0.004）。结论寻常痤疮患者囊肿中IL-6 mRNA水平显著升高。痤疮丙酸杆菌体外可激活人THP-1细胞信号分子p38MAPK，促进其分泌IL-6。

关键词：寻常痤疮痤疮丙酸杆菌白细胞介素6 白血病单核细胞急性 p38丝裂原活化蛋白激酶类

Analysis of clinical data of inpatients with primary hyperparathyroidism

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Analysis of clinical data of inpatients with primary hyperpa...

"六个下功夫":幼儿德育的指导思想和行动指南

International Conference on Osteoporosis and Bone Research 2008

作者： Xiaoyuan gong chunyan Lu Qin Wang Decai Chen Department of Endocrinology West China Hospital Sichuan University Chengdu China

＜正＞Objective:To summarize the clinical and laboratory presentations of primary hyperparathyroidism （PHPT） in West China Hospital, contributing to an increasing awareness about the disease. Methods:Reports of 63 p...

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科教导刊 2019年第34期 150-151页

作者：龚春艳南京师范大学泰州学院江苏泰州225300

2018年全国教育大会上指出,教育必须把培养社会主义建设者和接班人作为根本任务,并提出了"六个下功夫"重要的方法论.幼儿期是一个人道德、个性、习惯等开始形成的重要时期,这个时期给予幼儿良好的品德教育,将为培养有理想、有道德、有... 详细信息

2018年全国教育大会上指出,教育必须把培养社会主义建设者和接班人作为根本任务,并提出了"六个下功夫"重要的方法论.幼儿期是一个人道德、个性、习惯等开始形成的重要时期,这个时期给予幼儿良好的品德教育,将为培养有理想、有道德、有文化、有纪律的一代新人,培养社会主义建设者和接班人打下坚实的基础."六个下功夫"将成为幼儿德育的指导思想和行动指南.

关键词： "六个下功夫"幼儿德育指导思想行动指南