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Genetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed(Brassica napus)

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Oil Crop Science 2021年第4期6卷 169-174页

作者： Xiaohan Zhang Jinglong Lian chunyan dai Xiaoli Wang Mingzheng Zhang Xing Su Yaohua Cheng Chengyu Yu College of Agronomy Northwest A&F UniversityTaicheng Road 3YanglingShaanxi712100China Institute of Agricultural Sciences Garze Prefecture.Lucheng South Road 88KangdingSichuan626000China

Alpha linolenic acid(ALA)is an essential polyunsaturated fatty acids that can improve human *** is the second largest oil crop in the world but the ALA content in its seed fatty acids is only about 10%.Two rapeseed germplasms YH25005 and R8Q10 with high content(up to 21%)ALA were developed by intervarietal *** were used as the maternal parent(P_(1))when crossed with a low ALA parent SW(P2)to produce the seeds of the F1 hybrid,F2,and backcrosses to P1(BCP1)and P2(BCP2).A multigeneration joint segregation analysis was conducted to determine major gene t polygene effects of the content of three major unsaturated fatty acids including oleic(OA),linoleic(LA)and *** results showed that,although some genes favorable to ALA accumulation were not allelic in R8Q10 and YH25005,all the inheritances of OA,LA and ALA in YH25005×SW and R8Q10×SW followed a genetic model of‘two pairs of additive major genes t additive-dominant polygenes’.It is suggested that the contents of OA,LA and ALA are closely related and the major genes in the parent SW containing loss-of-function mutations in FAD2 and FAD3 loci had strong effect to reduce ALA and elevate ***,total genetic effect of the polygenes was greater than that of the two major genes,especial on ALA *** indicated that it is necessary to employ a larger F2 population to find the plant that accumulate enough minor-effect polygenes for high ALA *** results are useful for high ALA rapeseed breeding and future work of gene mapping.

关键词： Rapeseed High linolenicacid Fattyacid Genetic analysis Major gene Polygene

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虚拟项目团队中信任保障机制框架模型研究

科技进步与对策 2005年第8期22卷 18-20页

作者：代春艳重庆大学经济与工商管理学院重庆400044

在研究虚拟项目中信任的生命周期的基础上,提出了基于认证的网络声誉模型库的信任保障机制框架模型,为有效建立、巩固和发展虚拟项目团队中的信任提供了可行方案。

关键词：虚拟项目团队信任机制声誉模型

RNA demethylase ALKBH5 prevents colon adenocarcinoma progression by posttranscriptional activation of PER1 in an m6A-YTHDF2-dependent manner

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RNA demethylase ALKBH5 prevents colon adenocarcinoma progres...

Identification of potential core genes in colorectal cancer using bioinformatics analysis

2021年中国肿瘤标志物学术大会暨第十五届肿瘤标志物青年科学家论坛

作者： Mengyue Tian Xinghui Zhuang Zhiwen Zhao chunyan dai Fujian Medical University 福建医科大学附属协和医院

Background: N6-methyladenosine(m6 A) is the most abundant reversible methylation modification of eukaryotic mR NA, and it plays vital roles in tumourigenesis. This study aimed to explore the role of the m6 A demethylase ALKBH5 in colon adenocarcinoma(COAD).Methods: The expression of ALKBH5 and its clinicopathological impact were evaluated in COAD *** effects of ALKBH5 on the biological characteristics of COAD cells were investigated on the basis of gain-of-function and loss-of-function analyses. Subcutaneous and orthotopic models further uncovered the role of ALKBH5 in tumour growth. mR NA and m6 A sequencing and assays of m6 A methylated RNA immunoprecipitation-qPCR(MeRIP-qPCR) were performed to identify the targeted effect of ALKBH5 on PER1. P53-binding sites in the ALKBH5 promoter were investigated by ChIP and luciferase assays to reveal the interplay between ALKBH5 and PER1-activated ATM-CHK2-P53/CDC25 C ***: ALKBH5 loss characterized the occurrence and poor clinicopathological manifestations in patients with COAD. Overexpression of ALKBH5 reduced tumoural proliferative, migrative, invasive activities in vitro and ameliorated tumour growth in vivo, whereas ALKBH5 knockdown facilitated COAD progression. Mechanistically, ALKBH5 posttranscriptionally activated PER1 by m6 A demethylation in an m6 A-YTHDF2-dependent manner. PER1 upregulation led to the reactivation of ATM-CHK2-P53/CDC25 C signaling, which inhibited cell growth. P53-induced activation of ALKBH5 transcription acted as a feedback loop regulating the m6 A modifications in ***: ALKBH5 serves as a COAD suppressor by regulating the posttranscriptional activation of PER1 through m6 A abolishment, which may highlight a demethylation-based approach for COAD diagnosis and therapy.

关键词： colon adenocarcinoma m6A ALKBH5 PER1

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Identification of potential core genes in colorectal cancer ...

Ytterbium ion promotes apoptosis of primary mouse bone marrow stromal cells

2021年中国肿瘤标志物学术大会暨第十五届肿瘤标志物青年科学家论坛

作者： Mengyue Tian Xinghui Zhuang chunyan dai Zhiwen Zhao Fujian Medical University 福建医科大学附属协和医院

Background: Colorectal cancer(CRC) is a common human malignancy worldwide. The tumorigenesis mechanism in CRC is *** and methods: To explore potential therapeutic targets for CRC, we analyzed 3 microarray datasets(GSE20347, GSE38129, and GSE67269) derived from the gene expression omnibus(GEO)database. Then, the GEO2 R tool was used to screen out differently expressed genes(DEGs) between CRC and normal tissue. Gene ontology function and kyoto encyclopedia of genes and genomes pathway enrichment analysis were performed using the database for annotation, visualization and integrated discovery to identify the pathways and functional annotation of DEGs. Protein–protein interaction of these DEGs was analyzed based on the search tool for the retrieval of interacting genes database and visualized by Cytoscape software. In addition, we used encyclopedia of RNA interactomes(ENCORI), gene expression profiling interactive analysis(GEPIA), and the human protein atlas to confirm the expression of hub genes in CRC. Finally, GEPIA was used to evaluate the prognostic value of hub genes expression in CRC patients and we estimated the associations between hub genes expression and immune cell populations(B Cell, CD8+T Cell, CD4+T Cell, Macrophage,Neutrophil, and Dendritic Cell) in CRC using tumor immune estimation resource(TIMER).Results: In this study, 707 DEGs(including 385 upregulated genes and 322 downregulated genes) and 6 hub genes(cyclin B1 [CCNB1], cyclin dependent kinase 1 [CDK1], aurora kinase A [AURKA],ubiquitin conjugating enzyme E2 C [UBE2 C], cyclin A2 [CCNA2], and cell division cycle 20[CDC20]) were identified. All of the 6 hub genes were highly expressed in CRC tissues. Among of them, only CCNB1 and CDC20 were associated with stage of CRC and all of them were not associated with survival time of ***: DEGs and hub genes were confirmed in our study, providing a thorough, scientific and comprehensive research goals for the pathogenesis of CRC.

关键词： colorectal cancer hub genes expression profiling data bioinformatics analysis differently expressed genes

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Journal of Rare Earths 2015年第4期33卷 445-452页

作者：戴春燕陈士柱王朝张良葛昆张金超 Chemical Biology Key Laboratory of Hebei Province College of Chemistry & Environmental Science Hebei University Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education Hebei University Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education College of Chemistry & Chemical Engineering Hainan Normal University

One of the main target organs for the lanthanides （Ln） is bone. Previous studies revealed that ytterbium （Yb） produced damage to the skeletal system in vivo. But the effects of Yb3＋ on bone marrow stromal cells （BMSCs） in vitro had not been reported. In this paper, cell viability, apoptosis, mitochondrial membrane potential （MMP）, reactive oxygen species （ROS） and lactate dehy- drogenase （LDH） were measured in order to study the effects of Yb3＋ on BMSCs. The results indicated that Yb3＋ displayed a slight positive effect on the BMSCs viability at concentrations of 1 x 104, 1 × 105, and 1 x 104 mol/L, but turned to decrease the viability of BMSCs at the highest concentration of 1 × 103 mol/L for 24, 48 and 72 h. Yb3＋ at 1 x 103 mol/L promoted apoptosis of BMSCs, in- creased the levels of ROS and LDH, and decreased MMP in BMSCs. It suggested that the precipitate of YbPO4 might decrease the viability of BMSCs. Yb3＋ induced the apoptosis of BMSCs via mitochondrial pathway. The results might be useful for more rational application of Yb-based compounds in the future.

关键词： ytterbium bone marrow stromal cells precipitation apoptosis rare earths

Blocking the JAK2/STAT3 and ERK pathways suppresses the proliferation of gastrointestinal cancers by inducing apoptosis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2021年第6期22卷 492-503页

作者： Xi WANG chunyan dai Yifei YIN Lin WU Weiyang JIN Yufei FU Zhe CHEN Ke HAO Bin LU Key Laboratory of Digestive Pathophysiology of Zhejiang Province the First Affiliated Hospital of Zhejiang Chinese Medical UniversityZhejiang Chinese Medical UniversityHangzhou 310006China College of Life and Environmental Sciences Hangzhou Normal UniversityHangzhou 310036China Research Center of Blood Transfusion Medicine Ministry of Education Key Laboratory of Laboratory MedicineDepartment of Blood TransfusionZhejiang Provincial People's HospitalPeople's Hospital of Hangzhou Medical CollegeHangzhou 310014China

Dysregulated crosstalk between different signaling pathways contributes to tumor development,including resistance to cancer *** the present study,we found that the mitogen-activated extracellular signal-regulated kinase(MEK)inhibitor trametinib failed to suppress the proliferation of PANC-1 and MGC803 cells by activating the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway,while the JAK2 inhibitor fedratinib failed to inhibit the growth of the PANC-1 cells upon stimulation of extracellular signal-regulated kinase(ERK)*** particular,the most prominent enhancement of the anti-proliferative effect resulted from the concurrent blockage of the JAK2/STAT3 and ERK signaling ***,the combination of the two inhibitors resulted in a reduced tumor burden in *** evidence suggests novel crosstalk between JAK2/STAT3 and ERK signaling in gastric cancer(GC)and pancreatic ductal adenocarcinoma(PDAC)cells and provides a therapeutic strategy to overcome potential resistance in gastrointestinal cancer.

关键词： Gastrointestinal cancers JAK2/STAT3 pathway ERK pathway Crosstalk Apoptosis

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协同式超媒体智能计算机辅助教学系统设计

西南师范大学学报（自然科学版） 1999年第2期24卷 162-166页

作者：代春艳张小真西南师范大学计算机科学系重庆400715

提出了一个集智能化、超媒体化、协作化三种特性为一体的协同式超媒体智能计算机辅助教学系统(CHICAI)模型.给出了该系统采用的教学模式、系统的多Agent结构,并介绍了系统功能.着重讨论了系统/教学管理的实施。

关键词：人工智能超媒体 CAI CHICAI 设计

不确定性信息下的可再生能源规划决策路径研究

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生态经济 2014年第1期30卷 20-22,73页

作者：代春艳张希良重庆工商大学管理学院重庆400067 清华大学能源环境经济研究所北京100084

不确定性是可再生能源规划系统的一个重要特征。不确定性如何发生,如何掌握和运用不确定性,如何在不确定条件下得出结论以及处理不确定性至关重要。因此提出树立"不确定性"可再生能源规划决策思想观念,讨论不确定视角下的可再生能源思... 详细信息

不确定性是可再生能源规划系统的一个重要特征。不确定性如何发生,如何掌握和运用不确定性,如何在不确定条件下得出结论以及处理不确定性至关重要。因此提出树立"不确定性"可再生能源规划决策思想观念,讨论不确定视角下的可再生能源思想体系,提出不确定视角下的可再生能源规划方法与思路。

关键词：不确定性可再生能源规划

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基于复杂系统科学的区域能源安全分析框架

科技管理研究 2014年第7期34卷 40-43,48页

作者：王胜王恩创代春艳李春艳重庆社会科学院重庆400020 重庆理工大学管理学院重庆400054 重庆工商大学管理学院重庆400067

在能源总量控制和温室气体减排双重驱动下,区域(省市)能源安全成为学术研究和政策制定者关注的热点。基于复杂系统理论,在界定区域能源安全内涵的基础上,系统阐述区域能源安全关键要素及其复杂性特征表现。借助计算机仿真工具建立基于... 详细信息

在能源总量控制和温室气体减排双重驱动下,区域(省市)能源安全成为学术研究和政策制定者关注的热点。基于复杂系统理论,在界定区域能源安全内涵的基础上,系统阐述区域能源安全关键要素及其复杂性特征表现。借助计算机仿真工具建立基于复杂系统科学的区域能源安全分析框架并给出框架的设计过程和实现方法,旨为考察区域能源消费总量及强度"双控制"下的区域能源安全状态及演进趋势,以期为管理者提供理论依据及决策支持。

关键词：区域能源安全复杂系统双控制动力学模型