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Chinese Chemical Letters 2022年第4期33卷 2073-2076页

作者： Chenglong Sun chunxia ma Lili Li Yuhao Han Daijie Wang Xiao Wan School of Pharmaceutical Sciences Qilu University of Technology(Shandong Academy of Sciences)Ji’nan 250014China Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province Shandong Analysis and Test CenterQilu University of Technology(Shandong Academy of Sciences)Ji’nan 250014China

Application of matrix-assisted laser desorption/ionization mass spectrometry imaging(maLDI-MSI) to investigate the spatiotemporal alterations of lipids in biological tissues has brought many significant results.However, the presence of structural isomers varying in C=C double bond(DB) locations makes isomerresolved MSI an urgent need. Herein, we introduce a new type of light-driven on-tissue [2 + 2] cycloaddition reaction coupled with maLDI-MS/MS imaging to identify lipid DB position isomers and their spatial signatures in biological tissues. 3-Benzoylpyridine was introduced as a novel derivatization reagent, and it exhibited great reactivity toward lipid C=C bond to form oxetanes under both ultraviolet light and visible light irradiation. With this approach, DB position isomers of lipids were imaged with highly differential levels in distinct regions of rat brain, providing an accurate and spatially resolved approach to study tissue lipidomics.

关键词： maLDI-MSI On-tissue cycloaddition Lipid C=C position isomers Biological tissues

Super-assembled sandwich-like Au@MSN@Ag nanomatrices for high-throughput and efficient detection of small biomolecules

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Nano Research 2022年第3期15卷 2722-2733页

作者： Dantong Zhao chunxia ma Meng Gao Yong Li Bo Yang Hui Li Runhao Zhang Minglu Hao Jing Huang Kang Liang Pu Chen Lei Xie Rong Rong Biao Kong College of Medicine Shandong University of Traditional Chinese MedicineJinan 250355China Shandong Analysis and Test Center National Supercomputer Research Center of Advanced MaterialsAdvanced Materials InstituteQilu University of Technology(Shandong Academy of Sciences)Jinan 250014China Heze Institute for Food and Drug Control Heze 274000China School of Chemical Engineering and Graduate School of Biomedical Engineering The University of New South WalesNSW 2052Australia Department of Chemical Engineering University of WaterlooWaterlooOntarioN2L 3G1Canada Department of Chemistry Shanghai Key Lab of Molecular Catalysis and Innovative MaterialsiChEMFudan UniversityShanghai 200032China

Small biomolecules(m/z<500)are the material basis of organisms and participate in life activities,but their comprehensive and accurate detection in complex samples remains a challenge.matrix-assisted laser desorption/... 详细信息

Small biomolecules(m/zmaterial basis of organisms and participate in life activities,but their comprehensive and accurate detection in complex samples remains a challenge.matrix-assisted laser desorption/ionization mass spectrometry(maLDI-MS)is a powerful detection tool for molecular analysis with high throughput.The development of a new matrix is essential to improve the efficiency of the maLDI-MS for molecular compound detection.In this work,the sandwich-like gold nanoparticles@mesoporous silica nanocomposite@silver nanoparticles(Au@MSN@Ag)nanospheres were prepared by layer-by-layer super-assembly strategy,and can be used as a novel matrix for the quantitative detection and enrichment of small biomolecules by LDI-MS.The sandwich-like nanospheres form a unique plasma resonant cavity that effectively absorbs the laser energy,while the homogeneous mesoporous structure of MSN can lock the analyte,which is essential for efficient LDI of small molecules.Compared to traditional matrices,Au@MSN@Ag shows the advantages of low background,wide application range,high sensitivity,super high salt and protein tolerance,and good stability.For example,the detection limit of glucose was as low as 5 fmol,and showed a good linear relationship in the range of 1−750μg/mL.Au@MSN@Ag assisted LDI-MS allows the enrichment and detection of small molecules in traditional Chinese medicine(TCM)without derivatization and purification,classification of herbs using the accurate quantitative results oligosaccharides,and identification of gelatin by amino acid content.This research could help in designing more efficient nanostructure matrices and further explored the application of LDI-MS.

关键词： matrix-assisted laser desorption/ionization mass spectrometry(maLDI-MS) matrix sandwich-like nanostructures small biomolecules traditional Chinese medicine(TCM)

急性单核细胞白血病的临床特点及预后因素分析

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实用癌症杂志 2024年第4期39卷 576-578,597页

作者：刘利红米瑞华马春霞郑州大学附属肿瘤医院河南省肿瘤医院450000

目的分析急性单核细胞白血病(AML-M5)患者的临床特点与其预后的相关因素。方法回顾性分析97例AML-M5患者并随访1年,依据其临床结果(死亡或生存)分为预后良好组与预后不良组,收集所有患者的年龄、性别等资料,采用多因素Logistic回归分析... 详细信息

目的分析急性单核细胞白血病(AML-M5)患者的临床特点与其预后的相关因素。方法回顾性分析97例AML-M5患者并随访1年,依据其临床结果(死亡或生存)分为预后良好组与预后不良组,收集所有患者的年龄、性别等资料,采用多因素Logistic回归分析模型分析影响AML-M5患者预后的相关因素。结果97例患者中,以发热为就诊原因的共51例(52.58%),其次为头晕乏力18例(18.56%)、牙龈肿痛及口腔溃疡14例(14.43%)等;骨髓增生程度分级以极度活跃为主,共47例(48.45%),其次为显著活跃20例(20.62%),活跃17例(17.53%),减低9例(9.28%),极度减低4例(4.12%)。随访1年,97例患者中生存80例(82.47%),死亡17例(17.53%);单因素分析显示:年龄、微小残留灶有无、功能状态评分(PS)、初治时白细胞计数与AML-M5患者预后有关(P50×10^(9)/L为AML-M5患者预后的独立影响因素(P50×10^(9)/L等。因此,临床需对上述高危人群予以重视,并施以个体化的防治手段。

关键词：急性单核细胞白血病预后影响因素

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疏勒河流域径流量和输沙量变化规律

水土保持研究 2024年第1期31卷 144-150页

作者：魏霞杨正华张振鹏路志强孙超王强牟极马春霞兰州大学资源环境学院兰州730000 甘肃省水利厅水土保持中心兰州730020 甘肃省水文站兰州730000

[目的]探索疏勒河流域水沙变化特性,可为国家实施西部生态安全战略提供科学支撑。[方法]基于疏勒河流域昌马堡、潘家庄、党城湾3个水文站的实测径流量和输沙量序列资料,运用滑动平均、mann-Kendall突变趋势检验和小波分析法等方法,分析... 详细信息

[目的]探索疏勒河流域水沙变化特性,可为国家实施西部生态安全战略提供科学支撑。[方法]基于疏勒河流域昌马堡、潘家庄、党城湾3个水文站的实测径流量和输沙量序列资料,运用滑动平均、mann-Kendall突变趋势检验和小波分析法等方法,分析了疏勒河流域径流量和输沙量变化规律。[结果]疏勒河流域年径流量和年输沙量均呈递增趋势,且输沙量增大趋势大于径流量,昌马堡、潘家庄、党城湾3个水文站径流量分别在1999年、2016年、1982年发生突变,昌马堡和潘家庄年输沙量突变均发生在1998年,党城湾输沙量没有突变;疏勒河流域3个代表站的径流量和输沙量都呈现出多时间尺度的演化特征,昌马堡径流量主周期为58 a,31 a,14 a,9 a和5 a,输沙量主周期为48 a,23 a,14 a,7 a和5 a,潘家庄径流量主周期为59 a,32 a,14 a,输沙量主周期为37 a,14 a,8 a和5 a,党城湾径流量主周期为42 a和5 a,输沙量主周期为16 a和8 a。[结论]疏勒河流域水沙总体呈递增趋势,且存在明显的周期变化,除党城湾的输沙量没有突变发生以外,其余站点水沙均有突变发生,同一水文站输沙量的突变的发生滞后于径流量的突变。

关键词： M-K检验法小波分析径流量输沙量疏勒河

Professor Xiaoquan Du's Seven Methods of Treating Stomachache

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Proceedings of Anticancer Research 2021年第4期5卷 117-120页

作者： Xuzhao Wang Li Liu Xiaoquan Du chunxia ma Wei Cui Shaanxi University of Chinese Medicine Xianyang 712046Shaanxi ProvinceChina The First Affiliated Hospital of Shaanxi University of Chinese Medicine Xianyang 712046Shaanxi ProvinceChina

Stomachache is the main symptom of stomach duct pain near the heart.Professor Xiaoquan Du is experienced in treating spleen and stomach diseases with traditional Chinese medicine(TCM)syndrome differentiation.He has vast practical TCM experience through years of clinical diagnosis and treatment,and incorporated his own characteristic methods in treatments,thereby developing seven methods for the treatment of stomachache.Clinically,methods such as“invigorating the spleen and benefiting the stomach”and“wanning the middle and dispersing cold”are mostly adopted to treat stomach duct pain.

关键词： Stomachache Treatment Traditional Chinese medicine

Professor Du Xiaoquan's Experience in Treating Acid Reflux

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Proceedings of Anticancer Research 2021年第4期5卷 108-111页

作者： Wei Cui Li Liu Xiaoquan Du chunxia ma Xuzhao Wang Shaanxi University of Chinese Medicine Xianyang 712046Shaanxi ProvinceChina The First Affiliated Hospital of Shaanxi University of Chinese Medicine Xianyang 712046Shaanxi ProvinceChina

Acid regurgitation is a common symptom in digestive system diseases which is mainly caused by emotions,diet,and other factors.It is related to gastroesophageal reflux disease,chronic gastritis,and other diseases in Western medicine.According to modem medical research,acid reflux is related to Helicobacter pylori(HP)infection,weak effect of anti-reflux barrier,decreased clearance of esophageal acid,dysfunction of esophageal mucosal barrier,strong stimulation of reflux on esophageal mucosal wall,and other factors[1].It can be divided into four types which are heat,cold,food stagnation,and yin deficiency It is often accompanied by vomiting,heartbum,stomachache,and other symptoms.Clinically,it is a mixture of cold and heat,as well as excess and deficiency.Professor Du's self-made method of“first soothing,second reducing,and third harmonizmg”(soothing liver and stomach,relieving stomach qi,and neutralizing stomach acid)is used to treat both,symptoms and the root causes.The commonly used prescriptions are left-running metal pill,cuttlefish bone and fritillaria powder,calcined ark shell,oyster shell,etc.

关键词： Acid regurgitation Stomach Cold

Study on the Rule of Acupoint Selection in Acupuncture and Moxibustion Treatment of Stomachache

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Journal of Clinical and Nursing Research 2021年第4期5卷 90-92页

作者： chunxia ma Li Liu Xiaoquan Du Wei Cui Xuzhao Wang Shaanxi University of Traditional Chinese Medicine Xianyang 712046Shaanxi ProvinceChina Department of Gastroenterology Affiliated Hospital of Shaanxi University of Traditional Chinese MedicineXianyang 712046Shaanxi ProvinceChina

A systematic collection of relevant literature on acupuncture and moxibustion treatment of stomach pain,and a summary of the rule of acupoints selection for acupuncture treatment of stomach pain.In conclusion,Zusanli,Zhongwan,Neiguan,and Liang Qiu are selected as the main acupoints.At the same time,according to the different syndrome types,dyspepsia hurt a stomach syndrome is compatible with Burong,Chengman,Tianshu and Shangjuxu acupoints.Cold evil invading the stomach syndrome is compatible with Shenque and Qihai acupoints.Liver-Qi attacking stomach syndrome is compatible with Danzhong,Zhangmen,and Qimen acupoints;for blood stasis to stop the stomach,it is compatible with Xuehai,Geshu,and Pishu acupoints.Adopting this rule of acupoints selection has a significant effect on acupuncture treatment of stomach pain,and it also provides a reference for other doctors to treat stomach pain.

关键词： Acupuncture Stomach pain Point selection

开放性眼外伤患者行Ⅱ期玻璃体切除手术的时机选择

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国际眼科杂志 2024年第4期24卷 630-633页

作者：马春霞杨霞霞田超伟李曼红胡丹王雨生张自峰空军军医大学西京医院眼科全军眼科研究所陕西省西安市710032

目的:观察开放性眼外伤不同时间点行玻璃体切除手术的临床疗效,以探讨Ⅱ期玻璃体手术的时机选择。方法:回顾性系列病例研究。纳入2022-06/2023-02就诊于我院眼科的开放性眼外伤患者60例60眼,按Ⅰ期规范化急诊救治手术时间与Ⅱ期玻璃体... 详细信息

目的:观察开放性眼外伤不同时间点行玻璃体切除手术的临床疗效,以探讨Ⅱ期玻璃体手术的时机选择。方法:回顾性系列病例研究。纳入2022-06/2023-02就诊于我院眼科的开放性眼外伤患者60例60眼,按Ⅰ期规范化急诊救治手术时间与Ⅱ期玻璃体视网膜手术的不同间隔时间分为治疗组A(间隔时间≤14 d, 32例32眼)和治疗组B(间隔时间>14 d, 28例28眼)。治疗组A中眼球破裂伤16眼(50%),穿通伤13眼(41%),贯通伤3眼(9%);治疗组B中眼球破裂伤15眼(54%),穿通伤12眼(43%),贯通伤1眼(4%)。术后随访6 mo,对比观察两组患者的治疗效果。结果:玻璃体切除手术前两组患者间视力无差异(P>0.05)。术后6 mo,治疗组A中10眼(31%)视力显著提高,21眼(66%)视力有效提高,1眼(3%)视力无改善;治疗组B中,5眼(18%)视力显著提高,16眼(57%)视力有效提高,7眼(25%)视力无变化,两组比较有差异(U=322.5,P=0.032)。治疗组A中继发性青光眼、硅油依赖、玻璃体再积血、眼球萎缩与治疗组B比较无显著差异(均P>0.05);治疗组A术后随访未见外伤性增生性玻璃体视网膜病变(TPVR),显著低于治疗组B(P<0.05)。结论:开放性眼外伤Ⅱ期玻璃体视网膜手术在Ⅰ期救治手术后2 wk内完成预后相对较好。

关键词：眼外伤开放性 Ⅱ期手术玻璃体切除术

The olfactory route is a potential way for SARS-CoV-2 to invade the central nervous system of rhesus monkeys

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Signal Transduction and Targeted Therapy 2021年第5期6卷 1614-1624页

作者： Li Jiao Yun Yang Wenhai Yu Yuan Zhao Haiting Long Jiahong Gao Kaiyun Ding chunxia ma Jingmei Li Siwen Zhao Haixuan Wang Haiyan Li Mengli Yang Jingwen Xu Junbin Wang Jing Yang Dexuan Kuang Fangyu Luo Xingli Qian Longjiang Xu Bin Yin Wei Liu Hongqi Liu Shuaiyao Lu Xiaozhong Peng National Kunming High-Level Biosafety Primate Research Center Institute of Medical BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeKunmingYunnanChina State Key Laboratory of Medical Molecular Biology Department of Molecular Biology and BiochemistryInstitute of Basic Medical SciencesMedical Primate Research CenterNeuroscience CenterChinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijingChina Department of Anatomy Institute of Basic Medical SciencesChinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijingChina

Neurological manifestations are frequently reported in the COVID-19 patients.Neuromechanism of SARS-CoV-2 remains to be elucidated.In this study,we explored the mechanisms of SARS-CoV-2 neurotropism via our established non-human primate model of COVID-19.In rhesus monkey,SARS-CoV-2 invades the CNS primarily via the olfactory bulb.Thereafter,viruses rapidly spread to functional areas of the central nervous system,such as hippocampus,thalamus,and medulla oblongata.The infection of SARS-CoV-2 induces the inflammation possibly by targeting neurons,microglia,and astrocytes in the CNS.Consistently,SARS-CoV-2 infects neuro-derived SK-N-SH,glial-derived U251,and brain microvascular endothelial cells in vitro.To our knowledge,this is the first experimental evidence of SARS-CoV-2 neuroinvasion in the NHP model,which provides important insights into the CNS-related pathogenesis of SARS-CoV-2.

关键词： invasion inflammation system

CD36 promotes tubular ferroptosis by regulating the ubiquitination of FSP1 in acute kidney injury

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Genes & Diseases 2024年第1期11卷 449-463页

作者： Yixin ma Lili Huang Zheng Zhang Pengfei Yang Qingsong Chen Xujia Zeng Fangyan Tan chunxia Wang Xiongzhong Ruan Xiaohui Liao Department of Nephrology The Second Affiliated HospitalChongqing Medical UniversityChongqing 400010China Department of Cell Biology and Genetics Chongqing Medical UniversityChongqing 400016China Centre for Nephrology Royal Free and University College Medical SchoolUniversity College LondonRoyal Free CampusRowland Hill StreetLondon NW32PFUnited Kingdom Centre for Lipid Research Key Laboratory of Molecular Biology on Infectious DiseasesMinistry of EducationChongqing Medical UniversityChongqing 400016China Kuanren Laboratory of Translational Lipidology Centre for Lipid ResearchSecond Affiliated Hospital of Chongqing Medical UniversityChongqing 400010China

Reactive oxidative species(ROos)production-driven ferroptosis plays a role in acute kidney injury(Akl).However,its exact molecular mechanism is poorly understood.Scavenger receptor CD36 has important roles in oxidizing lipids,lipid accumulation,metabolic syndrome,and insulin resistance in chronic kidney disease,but its roles remain unexplored in AKl.The present study investigated the role and mechanism of CD36 in regulating proximal tubular cell ferroptosis and AKl.The expression of CD36 was found to be significantly up-regulated in AKI renal tissues and correlated with renal function,which might serve as an independent biomarker for AKl patients.Moreover,in adult mice subjected to AKl,deletion of CD36(CD36-/-)induced tubular cell Ros accumulation,ferroptosis activation,and renal injury.Mechanistically,combining LC-MS/MS,co-IP,and ubiquitination analyses revealed that CD36 could specifically bind to ferroptosis suppressor protein 1(FSP1)and regulate its ubiquitination at sites K16 and K24,leading to FSP1 degradation and progression of ferroptosis in AKl.The present results emphasize a novel mechanism of CD36 in cisplatin-induced AKl.The discovery of the special CD36 roles in promoting ferroptosis and AKI development by regulating the ubiquitination of FSP1 in proximal tubular cells may be potential therapeutic targets for AKl.Moreover,CD36 may play a key role in the progression of AKl.Therefore,targeting CD36 may provide a promising treatment option for AKI.

关键词： Acute kidney injury CD36 Ferroptosis FSP1 Ubiquitin-dependent degradation