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Chinese Science Bulletin 2008年第21期53卷 3422-3424页

作者： dong chunxia ZHAO Jindong State Key Lab of Protein and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China

Pbycobilisomes(PBS) are able to transfer absorbed energy to photosystem I and Ⅱ,and the distribution of light energy between two photosystems is regulated by state *** this study we show that energy transfer from PBS to photosystem I(PSI) requires *** were unable to perform state transitions in the absence of *** apcD mutant grows more slowly in light mainly absorbed by PBS,indicating that ApcD-dependent energy transfer to PSI is required for optimal growth under this *** apcD mutant showed normal blue-light induced quenching,suggesting that ApcD is not required for this process and state transitions are independent of blue-light induced *** nitrogen fixing condition,the growth rates of the wild type and the mutant were the same,indicating that energy transfer from PBS to PSI in heterocysts was not required for nitrogen fixation.

关键词：蓝藻细菌光合体系藻胆蛋白体状态转移

Zein as a structural protein in gluten-free systems:an overview

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Food Science and Human Wellness 2021年第3期10卷 270-277页

作者： Xinrui Zhang chunxia dong Yayun Hu Mengnan Gao Guangzhong Luan College of Food Science and Engineering Northwest A&F UniversityYangling 712100China

Zein,a class of alcohol-soluble prolamines in maize endosperm,is mainly composed of α-zein,β-zein,andγ-*** has been recognized as a structural protein for various gluten-free systems since it can form glutenlike viscoelastic *** formation of viscoelastic zein network can make up for the structural defect of gluten-free doughs caused by the lack of *** make the most of structural functionality of zein in glutenfree foods,it is important to clearly elucidate the fundamental properties of zein *** this article,these properties have been discussed,analyzed and summarized from the relationship between protein network and structural functionality of zein,the feature and formation mechanism of zein network,factors affecting zein network and the applications of zein network in improving the quality of gluten-free *** addition,this article also looks forward to potential research areas on zein network.

关键词： Zein Gluten-free Network Glass transition

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布鲁氏菌LPS单克隆抗体的制备与鉴定

重庆理工大学学报（自然科学） 2024年第8期38卷 291-297页

作者：廖倩向梦玲凌洪权董春霞骆璐吴胜昔曾政重庆理工大学药学与生物工程学院重庆400054 重庆市动物疫病预防控制中心重庆401120

制备布鲁氏菌LPS特异性单克隆抗体,为建立布鲁氏菌病血清学及新型免疫传感器检测方法提供有效试剂。将天然提取的布鲁氏菌LPS抗原使用长程免疫法腹腔注射BALB/c小鼠,经过5次常规免疫及1次加强免疫后,取小鼠脾脏细胞与小鼠骨髓瘤细胞(SP2... 详细信息

制备布鲁氏菌LPS特异性单克隆抗体,为建立布鲁氏菌病血清学及新型免疫传感器检测方法提供有效试剂。将天然提取的布鲁氏菌LPS抗原使用长程免疫法腹腔注射BALB/c小鼠,经过5次常规免疫及1次加强免疫后,取小鼠脾脏细胞与小鼠骨髓瘤细胞(SP2/0)融合,使用间接酶联免疫吸附试验(iELISA)对杂交瘤细胞进行筛选,并对纯化后的单克隆抗体进行生物特性鉴定。结果表明:筛选出3株稳定分泌单抗的阳性细胞株,分别命名为2-4A、3-4E、5-10B;经过亚型鉴定,3-8E、2-4A为IgG1亚型,5-10B为IgG2b亚型。将5-10B细胞株使用腹腔注射法制备腹水,并通过Protein A柱纯化腹水。纯化后单抗达到了电泳纯,BCA法测定其浓度最高可达1.431 8 mg·mL^(-1), ELISA效价达到1∶512 000,间接ELISA结果显示制备的抗体具有良好的特异性。研究制备的高特异性、高活性的单克隆抗体可为后续布鲁氏菌病特异性诊断技术研究提供参考。

关键词：布鲁氏菌 LPS 细胞融合单克隆抗体生物特性鉴定

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弓形虫表面主要抗原1(SAG1)蛋白的原核表达与鉴定

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黑龙江畜牧兽医 2024年第12期 69-73,128页

作者：代琴骆璐董春霞凌洪权吴胜昔曾政秦萍韦广苗重庆理工大学药学与生物工程学院重庆400054 重庆市动物疫病预防与控制中心重庆401120

为了实现弓形虫主要表面抗原1(surface antigen 1,SAG1)蛋白在原核表达系统中的高效表达,试验根据GenBank中弓形虫SAG1基因序列(登录号为S76248.1)及大肠杆菌密码子偏好性对目的基因序列进行优化设计,以pET-32a(+)为表达载体构建重组表... 详细信息

为了实现弓形虫主要表面抗原1(surface antigen 1,SAG1)蛋白在原核表达系统中的高效表达,试验根据GenBank中弓形虫SAG1基因序列(登录号为S76248.1)及大肠杆菌密码子偏好性对目的基因序列进行优化设计,以pET-32a(+)为表达载体构建重组表达质粒pET32a(+)-SAG1,并将其转化至大肠杆菌BL21(DE3)感受态细胞中,用异丙基-β-D-硫代吡喃半乳糖苷(IPTG)对重组菌进行诱导,表达重组蛋白,通过优化诱导条件实现对重组蛋白的大量表达,使用NGC^(TM)层析纯化系统纯化重组蛋白,并分别以抗His标签单克隆抗体和犬弓形虫阳性血清作为一抗、羊抗鼠IgG-HRP和HRP-兔抗犬作为二抗对重组蛋白进行Western-blot鉴定。结果表明:重组表达质粒pET32a(+)-SAG1经双酶切分别在5 900 bp和1 020 bp处出现1条载体条带和1条目的基因条带,与预期结果相符;重组蛋白大小为55 ku且以包涵体的形式成功表达,当诱导条件为25℃、0.4 mmol/L IPTG诱导8 h时重组蛋白的表达量最高;纯化后的重组蛋白条带单一,纯度较高,3个样品质量浓度分别为0.409 5,0.368 5,0.451 9 mg/mL;表达的重组蛋白与抗His标签单克隆抗体和犬弓形虫阳性血清均能特异性结合。说明在大肠杆菌中成功表达了弓形虫SAG1重组蛋白,纯化后的重组蛋白纯度和质量浓度较高,且具有良好的反应原性。

关键词：弓形虫 SAG1 原核表达镍柱纯化蛋白免疫印迹

PatU3 plays a central role in coordinating cell division and differentiation in pattern formation of filamentous cyanobacterium Nostoc *** 7120

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Science China(Life Sciences) 2023年第12期66卷 2896-2909页

作者： Lei Yin Zhenggao Zheng Yilin Li Xiying Li Dan Cheng chunxia dong Yixuan Liu Jindong Zhao State Key Laboratory of Protein and Plant Genetic Engineering School of Life SciencesPeking UniversityBeijing 100871China National Teaching Center for Experimental Biology School of Life SciencesPeking UniversityBeijing 100871China Institute of Hydrobiology Chinese Academy of SciencesWuhan 430072China

Spatial periodic signal for cell differentiation in some multicellular organisms is generated according to Turing's principle for pattern *** a dividing cell responds to the signal of differentiation is addressed with the filamentous cyanobacterium Nostoc *** 7120,which forms the patterned distribution of *** show that differentiation of a dividing cell was delayed until its division was completed and only one daughter cell became heterocyst.A mutant of patU3,which encodes an inhibitor of heterocyst formation,showed no such delay and formed heterocyst pairs from the daughter cells of cell division or dumbbell-shaped heterocysts from the cells undergoing *** patA mutant,which forms heterocysts only at the filament ends,restored intercalary heterocysts by a single nucleotide mutation of patU3,and double mutants of patU3/patA and patU3/hetF had the phenotypes of the patU3 *** provide evidence that HetF,which can degrade PatU3,is recruited to cell divisome through its C-terminal domain.A HetF mutant with its N-terminal peptidase domain but lacking the C-terminal domain could not prevent the formation of heterocyst pairs,suggesting that the divisome recruitment of HetF is needed to sequester HetF for the delay of differentiation in dividing *** study demonstrates that PatU3 plays a key role in celldivision coupled control of differentiation.

关键词： cyanobacteria heterocyst differentiation cell division pattern formation PatU3

Development and validation of an HPLC–MS/MS method to determine clopidogrel in human plasma

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Acta Pharmaceutica Sinica B 2016年第1期6卷 55-63页

作者： Gangyi Liu chunxia dong Weiwei Shen Xiaopei Lu Mengqi Zhang Yuzhou Gui Qinyi Zhou Chen Yu Central Laboratory Shanghai Clinical CenterChinese Academy of Sciences/Central LaboratoryShanghai Xuhui Central Hospital State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of Science

A quantitative method for clopidogrel using online-SPE tandem LC–MS/MS was developed and fully validated according to the well-established FDA *** method achieves adequate sensitivity for pharmacokinetic studies,with lower limit of quantifications(LLOQs)as low as 10 pg/m *** separations were performed on reversed phase columns Kromasil Eternity-2.5-C18-UHPLC for both *** electrospray ionization in multiple reaction monitoring(MRM)mode was employed for signal detection and a deuterated analogue(clopidogrel-d_4)was used as internal standard(IS).Adjustments in sample preparation,including introduction of an online-SPE system proved to be the most effective method to solve the analyte back-conversion in clinical *** clinical samples(two levels)were prepared and successfully used as real-sample quality control(QC)in the validation of back-conversion testing under different *** result showed that the real samples were stable in room temperature for 24 ***,precision,extraction recovery,matrix effect on spiked QC samples and stability tests on both spiked QCs and real sample QCs stored in different conditions met the acceptance *** online-SPE method was successfully applied to a bioequivalence study of75 mg single dose clopidogrel tablets in 48 healthy male subjects.

关键词： Clopidogrel Online-SPE LC–MS/MS Back-conversion Bioequivalence Deuterated analogue Real samples stability FDA guidelines

CsGDH2.1 negatively regulates theanine accumulation in late-spring tea plants (Camellia sinensis var. sinensis)

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Horticulture Research 2023年第1期10卷 240-248页

作者： Tingting Chen Jingzhen Ma Huiping Li Shijia Lin chunxia dong Yunxia Xie Xiaomei Yan Shupei Zhang Tianyuan Yang Xiaochun Wan Zhaoliang Zhang State Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural UniversityHefei 230036China

Theanine,a unique and the most abundant non-proteinogenic amino acid in tea plants,endows tea infusion with the umami taste and anti-stress *** content in tea correlates highly with green tea *** content in new shoots of tea plants is high in mid-spring and greatly decreases in late ***,how the decrease is regulated is largely *** a genetic screening,we observed that a yeast mutant,glutamate dehydrolase 2(gdh2),was hypersensitive to 40 mM theanine and accumulated more *** result implied a role of CsGDH2s in theanine accumulation in tea ***,we identified the two homologs of GDH2,CsGDH2.1 and CsGDH2.2,in tea *** complementation assay showed that the expression of CsGDH2.1 in yeast gdh2 mutant rescued the theanine hypersensitivity and hyperaccumulation of this *** localization and tissue-specific expression showed CsGDH2.1 localized in the mitochondria and highly expressed in young ***,CsGDH2.1 expression was low in early spring,and increased significantly in late spring,in the new shoots of tea *** results all support the idea that CsGDH2.1 regulates theanine accumulation in the new ***,the in vitro enzyme assay showed that CsGDH2.1 had glutamate catabolic activity,and knockdown of CsGDH2.1 expression increased glutamate and theanine accumulation in the new shoots of tea *** findings suggested that CsGDH2.1-mediated glutamate catabolism negatively regulates theanine accumulation in the new shoots in late spring,and provides a functional gene for improving late-spring green tea quality.

关键词： spring infusion finding

The tea plant CsLHT1 and CsLHT6 transporters take up amino acids, as a nitrogen source, from the soil of organic tea plantations

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Horticulture Research 2021年第1期8卷 2333-2344页

作者： Fang Li chunxia dong Tianyuan Yang Shilai Bao Wanping Fang William J.Lucas Zhaoliang Zhang State Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University HefeiAnhui 230036China College of Horticulture Nanjing Agricultural University Nanjing 210095China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China Department of Plant Biology College of Biological Sciences University of CaliforniaDavisCA 95616USA

Organic tea is more popular than conventional tea that originates from fertilized *** acids inorganic soils constitute a substantial pool nitrogen(N)available for ***,the amino-acid contents in soils of tea plantations and how tea plants take up these amino acids remain largely *** this study,we show that the amino-acid content in the soil of an organic tea plantation is significantly higher than that of a conventional tea ***,alanine,valine,and leucine were the most abundant amino acids in the soil of this tea *** 15 N-glutamate was fed to tea plants,it was efficiently absorbed and significantly increased the contents of other amino acids in the *** cloned seven CsLHT genes encoding amino-acid transporters and found that the expression of CsLHT1,CsLHT2,and CsLHT6 in the roots significantly increased upon glutamate ***,the expression of CsLHT1 or CsLHT6 in a yeast amino-acid uptake-defective mutant,22Δ10α,enabled growth on media with amino acids constituting the sole N ***-acid uptake assays indicated that CsLHT1 and CsLHT6 are H^(+)-dependent high-and low-affinity amino-acid transporters,*** further demonstrated that CsLHT1 and CsLHT6 are highly expressed in the roots and are localized to the plasma ***,overexpression of CsLHT1 and CsLHT6 in Arabidopsis significantly improved the uptake of exogenously supplied 15 N-glutamate and 15 *** together,our findings are consistent with the involvement of CsLHT1 and CsLHT6 in amino-acid uptake from the soil,which is particularly important for tea plants grown inorganic tea plantations.

关键词： roots inorganic const

以癫痫为首发症状的早发成年型齿状核红核苍白球路易体萎缩症一例

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中华神经科杂志 2021年第4期54卷 384-387页

作者：董春霞路爱军曹秉振胡怀强解放军联勤保障部队第九六〇医院神经内科济南250031 潍坊医学院 261053

报道1例以癫痫为首发症状的早发成年型齿状核红核苍白球路易体萎缩症(DRPLA)患者的临床、电生理、影像学及基因突变特点。该患者为女性,22岁起病,以癫痫为首发症状,逐渐出现小脑性共济失调、认知障碍、舞蹈样动作。脑电图见全头区多发... 详细信息

报道1例以癫痫为首发症状的早发成年型齿状核红核苍白球路易体萎缩症(DRPLA)患者的临床、电生理、影像学及基因突变特点。该患者为女性,22岁起病,以癫痫为首发症状,逐渐出现小脑性共济失调、认知障碍、舞蹈样动作。脑电图见全头区多发复形慢波。头颅磁共振成像见小脑、脑干、顶枕部皮质萎缩。患者外周血肌萎缩蛋白-1基因CAG重复次数为15/65次。其父亲肌萎缩蛋白-1基因CAG重复次数为14/54次,尚未出现临床症状。其母亲及弟弟基因检测正常。DRPLA临床表现复杂多样、表型异质性高,以癫痫起病的早发成年型少见,基因检测可以明确诊断。

关键词：共济失调癫痫齿状核红核苍白球路易体萎缩症

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石油降解菌的固定化材料研究进展

材料导报 2017年第A2期31卷 214-218页

作者：张武董春霞纪妍妍天津科技大学海洋与环境学院天津市海洋资源与化学重点实验室天津300457 天津工业大学环境与化学工程学院省部共建分离膜与膜过程国家重点实验室天津300387

随着微生物固定化技术在石油污染治理领域内发挥的作用越来越大,关于固定化微生物技术的研究越来越受到关注,而载体材料在微生物固定化技术中发挥着关键的作用。本文阐述了对固定化石油降解菌载体材料性能的要求,对无机物、天然有机物... 详细信息

随着微生物固定化技术在石油污染治理领域内发挥的作用越来越大,关于固定化微生物技术的研究越来越受到关注,而载体材料在微生物固定化技术中发挥着关键的作用。本文阐述了对固定化石油降解菌载体材料性能的要求,对无机物、天然有机物、合成高分子、复合材料等不同类型载体在石油降解菌固定化中的研究与应用以及对各类材料固定化石油降解菌的降解效果进行了综述。对比概述了各种不同载体材料对应的固定化方法,指出了各载体材料、相应固定方法的优劣势及适用场景,描述了石油降解菌固定化技术及相关载体材料今后的发展趋势。

关键词：石油降解菌固定化微生物载体材料