检索结果-南通市图书馆

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

多模态非线性光学显微成像技术研究综述

光学学报 2024年第4期44卷 32-55页

作者：李艳萍陈永强刘雨晴胡睿屈军乐刘丽炜深圳大学物理与光电工程学院教育部/广东省光电子器件与系统重点实验室广东深圳518060

介绍各种非线性光学显微成像的基本原理,并阐述非线性光学成像的多模态耦合所面临的技术挑战与解决方案。从成像速度、空间分辨率以及信噪比三个方面介绍了多模态非线性光学成像的研究进展,并扩展了多模态非线性光学内窥镜和图像分析方... 详细信息

介绍各种非线性光学显微成像的基本原理,并阐述非线性光学成像的多模态耦合所面临的技术挑战与解决方案。从成像速度、空间分辨率以及信噪比三个方面介绍了多模态非线性光学成像的研究进展,并扩展了多模态非线性光学内窥镜和图像分析方法。最后展望了多模态非线性光学成像的发展趋势和所面临的挑战,以期给相关领域研究人员提供参考。

关键词：成像系统显微成像非线性光学多模态光学成像光学内窥镜

New Quinoxaline-Based Blue Emitters: molecular Structures, Aggregation-Induced Enhanced Emission Characteristics and OLED Application

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2021年第8期39卷 2154-2162页

作者： Xiaoyu Mao Fuli Xie Xiaohui wang Qingsong wang Zhipeng Qiu Mark R.J.Elsegood Jie Bai Xing Feng Carl Redshaw yanping huo Jian-Yong hu Qing chen Guangdong Provincial key laboratory of Functional Soft Condensed Matter School of Material and EnergyGuangdong University of TechnologyGuangzhouGuangdong 510006China Shaanxi key laboratory for Advanced Energy Devices School of Materials Science and EngineeringShaanxi Normal UniversityXi'anShaanxi 710119China School of Chemical Engineering and Light Industry Guangdong University of TechnologyGuangzhouGuangdong 510006China Chemistry Department Loughborough UniversityLoughboroughLE113TUUK Department of Chemistry University of HullCottingham RoadHullYorkshire HU67RXUK Chinese Research Academy of Environmental Sciences No.8DayangfangBeiyuanBeijingChina

Three new deep blue light emitters,bearing quinoxaline with different substituents(biphenyl/pyrene)at the 1-or 2-position,were synthesized by a Pd-catalysed coupling reaction in high yields,and were fully characterize... 详细信息

关键词： Quinoxaline Pyrene Aggregation-induced emission Solid-state structures Substituent effects

鸡群中鸡传染性贫血病原与抗体检测及分离株VP1基因序列分析

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国家禽 2024年第2期46卷 52-58页

作者：冯笑艳胡明雪林雨萌刘长军李凯祁小乐崔红玉高立王素艳陈运通王笑梅张艳萍高玉龙中国农业科学院哈尔滨兽医研究所动物疫病防控全国重点实验室黑龙江哈尔滨150069

为了解鸡群中鸡传染性贫血病毒(CAV)感染及抗体产生情况,从黑龙江省两个无临床症状鸡群(B群和S群)中,分别采集血清通过ELISA方法进行抗体检测,采集抗凝血并分离淋巴细胞,针对基因组保守序列设计引物,通过PCR方法进行病原检测,并对从B群... 详细信息

为了解鸡群中鸡传染性贫血病毒(CAV)感染及抗体产生情况,从黑龙江省两个无临床症状鸡群(B群和S群)中,分别采集血清通过ELISA方法进行抗体检测,采集抗凝血并分离淋巴细胞,针对基因组保守序列设计引物,通过PCR方法进行病原检测,并对从B群病原阳性鸡分离到的分离株进行VP1基因序列分析。结果显示,B群和S群CAV抗体阳性率分别为62.7%(79/126)和68.2%(88/129),病原阳性率分别为43.0%(49/114)和62.3%(48/77),其中,B群和S群中抗体和病原双阴性的鸡分别为23.7%(27/114)和15.6%(12/77),而抗体和病原双阳性的鸡分别高达28.1%(32/114)和46.8%(36/77);在双阳性鸡中,B群和S群分别有62.5%(20/32)和58.3%(21/36)抗体滴度在1.0×10^(4)及以上,从B群病原阳性鸡全血中分离到一株CAV(HLJ/2022株),分离株HLJ/2022第394位氨基酸为谷氨酰胺,具有强毒的分子特征;两个鸡群的环境拭子CAV阳性率为10.0%(3/30),存在于消毒前的鸡蛋表面、更衣处和鞋底。研究表明,检测鸡群的CAV抗体阳性率在62.7%以上,病原阳性率在43.0%以上,抗体滴度在1.0×104及以上CAV仍可存在,CAV流行株(HLJ/2022)具有强毒分子特征,鸡群环境中存在CAV污染,结果为CIA防控提供重要的参考意见。

关键词：鸡传染性贫血病毒血清学检测病原学检测病毒分离鉴定 VP1基因序列分析

Jet-like features of Jiulongjiang River plume discharging into the west Taiwan Strait

在线全文

学校读者我要写书评

暂无评论

Frontiers of Earth Science 2013年第3期7卷 282-294页

作者： Daifeng wang Quan'an ZHENG Jianyu hu state key laboratory of Marine Environmental Science College of Ocean and Earth Sciences Xiamen University Xiamen 361005 China Department of Atmospheric and Oceanic Science University of Maryland College Park MD 20742 USA

In-situ data from the summer cruise of 2010 in the west Taiwan Strait are used to study the spatial distribution of the Jiulongjiang River plume （JRP）. The results show that in the 2 m layer, the JRP debouches into the west Taiwan Strait in the form of jets, with one branch through the Xiamen Bay （Xiamen JR_P） and another through the channel between Jinmen and Weitou （JinWei JRP）. Driven by the summer southwesterly monsoon, the upwelling-related Dongshan low temperature and high salinity water flows northeastward in the form of a jet as well. To a certain degree, the Dongshan low temperature and high salinity jet restricts the Xiamen JRP from spreading further offshore and drags the JinWei JRP northeastward at the same time. Meanwhile, a terrestrial dissolved organic matter （DOM） distribution model on the basis of molecular collision theory in thermodynamics and statistical physics is applied to analyze the Moderate Resolution Imaging Spectroradiometer （MODIS） turbidity data. The correlation coefficient of the theoretical model to the MODIS turbidity data reaches 0.96 （significant at a 95% level of confidence）. The result clarifies the dynamic mechanism for the turbidity distribution characteristics. It is the salinity in macro-scale that plays a decisive role in the turbidity variability in the coastal water. This suggests that the satellite-derived turbidity data can be used as an indicator to show the spreading patterns of the JRP. Based on the turbidity data from 2003 to 2011, we conclude that there are four main spreading patterns of the JRP.

关键词： Jiulongjiang River plume jet current terrestrial DOM MODIS data Taiwan Strait

清远麻鸡miR-142-3p分析及其关键靶基因的筛选

在线全文

学校读者我要写书评

暂无评论

中国农业大学学报 2024年第7期29卷 12-22页

作者：朱师良余春林邱莫寒张增荣熊霞胡陈明杨礼彭涵陈家磊宋小燕夏波杨朝武四川省畜牧科学研究院动物遗传育种重点实验室成都610066

为分析清远麻鸡不同组织中miR-142-3p的表达情况,并筛选其在脾脏中的关键靶基因,本研究利用在线数据库预测鸡miR-142-3p靶基因,并对靶基因进行GO和KEGG富集分析以及构建蛋白-蛋白互作网络。利用在线公共数据库,分析脾脏相对于其他组织的... 详细信息

为分析清远麻鸡不同组织中miR-142-3p的表达情况,并筛选其在脾脏中的关键靶基因,本研究利用在线数据库预测鸡miR-142-3p靶基因,并对靶基因进行GO和KEGG富集分析以及构建蛋白-蛋白互作网络。利用在线公共数据库,分析脾脏相对于其他组织的miR-142-3p表达量;再根据miRNA表达量与其靶基因表达量负相关机制,对miR-142-3p靶基因与脾脏中相对其他组织显著下调基因取交集,筛选潜在靶基因。结果表明:1)预测获得85个靶基因,GO富集分析显示,靶基因参与多种高分子化合物的合成与转运过程。2)KEGG结果显示富集到MAPK、Notch、Wnt等多条与抗肿瘤免疫相关的信号通路。3)PPI网络共筛选到4个hub基因。4)miR-142-3p在脾脏中的表达量极显著高于肝脏、肺脏、胸肌、心脏和肾脏(P1)。5)共筛选到TWF1、MYH10、NFIB、FNBP1L和FERMT25个靶基因。综上,鸡miR-142-3p在脾脏中很可能通过TWF1、MYH10、NFIB、FNBP1L和FERMT2发挥生物学功能。本研究为解析鸡miR-142-3p的功能及其分子机制提供了参考和依据。

关键词：清远麻鸡 miR-142-3p 生物信息学组织表达脾脏

Synthesis,characterization and cell imaging properties of rare earth compounds based on hydroxamate ligand

在线全文

学校读者我要写书评

暂无评论

Journal of Rare Earths 2018年第4期36卷 418-423页

作者： Linyan Yang yanping Zhang Liwei hu Yunhe Zong Ruili Zhao Tianming Jin Wen Gu College of Animal Science and Veterinary Medicine Tianjin Agricultural UniversityTianjin 300384China key laboratory for Chemistry and molecular Engineering of Medicinal Resources Guangxi Normal UniversityGuilin 541004China College of Chemistry Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)Nankai UniversityTianjin 300071China key laboratory of Advanced Energy Materials Chemistry(KLAEMC) Nankai UniversityTianjin 300071China

Six binuclear rare earth compounds were synthesized through the ligand of 4-Bromo-N-hydroxy-Nmethyl-benzamide（sH_2 bha）. Compounds 1-3 are isomorphous, and compound 1 is crystallized in the monoclinic system with the P2_1/c space group. Compounds 4 and 5 are isomorphous, and compound 4 is crystallized in the triclinic system with the P-1 space group. Compound 6 is crystallized in the triclinic system with the P-1 space group. The compounds were characterized by thermogravimetric analysis（TGA） and IR spectroscopy. The fluorescence spectrum in the visible area shows characteristic peaks of Eu, Tb, and Dy compounds. The efficiency of Eu compound on the viability of PC3 cells was assessed using CCK8 assays. From the CCK8 results, ionic concentration may have a great effect on PC3 cells＇ proliferation. Eu compound shows red fluorescence in the cytoplasm of PC3 cell. As for Eu compound, it might have potential application in cell imaging technology.

关键词： Rare earth Fluorescence Cell imaging

小兰屿蝴蝶兰YUCCA基因家族鉴定及在花朵中的表达分析

在线全文

学校读者我要写书评

暂无评论

分子植物育种 2024年第13期22卷 4177-4184页

作者：张燕萍童妍胡美娟曹映辉陈斌郑燕周小琴赵凯彭东辉周育真福建农林大学园林学院兰科植物保护与利用国家林业和草原局重点实验室福州350002 福建师范大学生命科学学院福州350117

生长素合成基因YUCCA在植物根、茎、叶的形成,花、果实、种子的发育等生命过程中起着重要作用。本试验针对小兰屿蝴蝶兰(Phalaenopsis equestris) YUCCA基因家族进行研究,鉴定得到8个PeYUCCAs家族成员。对其理化性质、系统进化树、保守... 详细信息

生长素合成基因YUCCA在植物根、茎、叶的形成,花、果实、种子的发育等生命过程中起着重要作用。本试验针对小兰屿蝴蝶兰(Phalaenopsis equestris) YUCCA基因家族进行研究,鉴定得到8个PeYUCCAs家族成员。对其理化性质、系统进化树、保守结构域及保守基序、基因结构、蛋白三维结构等生物信息进行分析,结果表明:氨基酸长度为400~426 aa,蛋白质分子量为43.22~47.92 kD,等电点为6.43~9.47;GAGPSG(FAD-binding)、GCGNSG (NADPH-binding)为Pe YUCCAs的保守结构域;同时其保守motif为16条,9条为所有成员所共有,且其基因结构存在2~4个内含子;PeYUCCAs的蛋白三维结构主要由多个α-螺旋、β-折叠及无规则卷曲组成;在构建的系统进化树中,YUCCA序列分为四个进化枝,GroupⅠ进一步分为两个亚类群,且PeYUCCAs成员在每个进化枝中都有分布。此外,RT-qPCR试验结果表明:PeYUCCA3在花朵不同时期及不同部位均有特异性表达,其中大蕾花期、合蕊柱花部的表达量最高;而PeYUCCA1、PeYUCCA5仅在合蕊柱花部中有高表达。由此推测PeYUCCA1、PeYUCCA3、PeYUCCA5可能在花器官生长发育过程,尤其在雌雄蕊发育中发挥着重要作用。

关键词：小兰屿蝴蝶兰 YUCCA 花发育实时荧光定量PCR

Safety and activity of WX-0593(Iruplinalkib)in patients with ALK-or ROS1-rearranged advanced non-small cell lung cancer:a phase 1 dose-escalation and dose-expansion trial

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第2期7卷 573-582页

作者： Yuankai Shi Jian Fang Xuezhi Hao Shucai Zhang Yunpeng Liu Lin wang Jianhua chen Yi hu Xiaosheng Hang Juan Li Chunling Liu Yiping Zhang Zhehai wang yanping hu Kangsheng Gu Jian’an huang Liangming Zhang Jinlu Shan Weiwei Ouyang Yanqiu Zhao Wu Zhuang Yan Yu Jun Zhao Helong Zhang Pei Lu Weidong Li Meimei Si Mingjing Ge huaize Geng National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing Key Laboratory of Clinical Study on Anticancer Molecular Targeted Drugs100021 BeijingP.R.China Beijing Cancer Hospital 100142 BeijingP.R.China Beijing Chest Hospital Capital Medical University101149 BeijingP.R.China The First Hospital of China Medical University 110001 ShenyangP.R.China hunan Cancer Hospital 410000 ChangshaP.R.China Chinese PLA General Hospital 100853 BeijingP.R.China Affiliated Hospital of Jiangnan University 214062 WuxiP.R.China Sichuan Cancer Hospital 610041 ChengduP.R.China Affiliated Tumour Hospital of Xinjiang Medical University 830000 UrumqiP.R.China Zhejiang Cancer Hospital 310022 HangzhouP.R.China Shandong Cancer Hospital and Institute Shandong First Medical University and Shandong Academy of Medical Sciences250117 JinanP.R.China hubei Cancer Hospital 430079 WuhanP.R.China The First Affiliated Hospital of Anhui Medical University 230022 HefeiP.R.China The First Affiliated Hospital of Soochow University 215006 SuzhouP.R.China Yantai Yuhuagding Hospital Yantai 264000P.R.China Army Medical Center of People’s Liberation Army 400042 ChongqingP.R.China Guizhou Cancer Hospital 520100 GuiyangP.R.China Henan Cancer Hospital 450008 ZhengzhouP.R.China Fujian Province Cancer Hospital 350014 FuzhouP.R.China Harbin Medical University Cancer Hospital 150081 HarbinP.R.China Tangdu Hospital of the Fourth Military Medical University 710038 Xi’anP.R.China Liuzhou People’s Hospital 545006 LiuzhouP.R.China Cancer Center of Guangzhou Medical University 510095 GuangzhouP.R.China Qilu Pharmaceutical Co. Ltd250101 JinanP.R.China

WX-0593(Iruplinalkib)is a novel,highly selective oral ALK and ROS1 tyrosine kinase inhibitor(TKI).In this study,the safety,antitumor activity,and pharmacokinetics of WX-0593 were evaluated in advanced non-small cell lung cancer(NSCLC)patients with ALK or ROS1 *** the dose-escalation phase and dose-expansion phase,patients were treated with WX-0593 until disease progression,unacceptable toxicity,or subject *** the dose-escalation phase,the primary endpoints were maximum tolerated dose(MTD),dose-limiting toxicity(DLT),and safety assessed by *** the dose-expansion phase,the primary endpoint was objective response rate(ORR)assessed by *** September 25,2017 and October 15,2018,a total of 153 patients received WX-0593 *** dose-limiting toxicities(DLTs)including one grade 3 QT interval prolonged and one grade 2 chronic heart failure were reported at the dose of 300 mg in one *** was not ***,140 of the 152(92%)patients experienced treatment-related adverse events(TRAEs)and 35 of the 152(23%)patients had TRAEs≥grade *** overall ORR was 59.3%(32 of 54)for the dose-escalation phase and 56.6%(56 of 99)for the dose-expansion *** patients who were ALK-rearranged and ALK TKI naive,the ORR were 81.0%(17 of 21)in the dose-escalation phase and 76.3%(29 of 38)in the dose-expansion phase,and for patients who previously received crizotinib as the only ALK TKI,the ORR were 38.1%(8 of 21)and 45.7%(21 of 46)for the two phases,*** patients who were ROS1-rearranged,the ORR were 30.0%(3 of 10)in the dose-escalation phase and 44.4%(4 of 9)in the dose-expansion ***-0593 showed favorable safety and promising antitumor activity in advanced NSCLC patients with ALK or ROS1 rearrangement.

关键词： patients expansion phase

Glypican-3 versus alpha-fetoprotein as a biomarker for hepatocellular carcinoma: a diagnostic meta-analysis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BIOCELL 2015年第2期39卷 25-31页

作者： Weiyu FU Hongyu LU Li LI Kefeng WU yanping LI Yi LIU Jianli hu Institute of Biochemistry and molecular biology Guangdong Medical UniversityZhanjiangGuangdongChina College of Food Science and Technology Guangdong Ocean UniversityZhanjiangGuangdongChina Guangdong key laboratory for Research and Development of Natural Drugs Guangdong Medical UniversityZhanjiangGuangdongChina Cancer Center Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiChina

Objective:To assess the diagnostic accuracy of serum GPC3 versus alpha-fetoprotein(AFP)for HCC by using the method of system ***:PubMed and EMBASE were searched from its inception to 20,April 2014 for studies that compared diagnostic accuracy of serum GPC3 with AFP for ***,specificity and other measures were pooled using random-effects *** receiver operating characteristic curve analysis was used to summarize the overall test ***:Fourteen studies were included in this *** estimates for serum GPC3 and AFP in diagnosing HCC were as follows:sensitivity,69%(95%confidence interval(CI),56-80%)vs.60%(95%CI,50-69%);specificity,91%(95%CI,76-97%)vs.92%(95%CI,84-98%);diagnostic odds ratio(DOR),22(95%CI,6-83)vs.18(95%CI,8-41);and area under sROC,0.85(95%CI,0.81-0.88)vs.0.80(95%CI,0.76-0.83).The pooled sensitivity and specificity for(GPC3+AFP)and AFP were:sensitivity 80%(95%CI,75-85%)vs.64%(95%CI,53-73%)and specificity 86%(95%CI,74-93%)vs.96%(95%CI,86-99%).A significant heterogeneity was found among the ten studies,and meta-regression and subgroup meta-analysis suggested that race and assay type were probably responsible for the ***:Serum GPC3 may be a promising diagnostic marker of HCC and it was helpful for early detection of primary hepatocellular carcinoma when combined with *** studies for specific race of patients,and using certain methods for detecting GPC3 are required to further confirm the diagnostic value of GPC3 for HCC.

关键词： Glypican-3 AFP Hepatocellular carcinoma diagnostic meta-analysis