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Antagonizing exosomal miR-18a-5p derived from prostate cancer cells ameliorates metastasis-induced osteoblastic lesions by targeting Hist1h2bc and activating Wnt/β-catenin pathway

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Genes & Diseases 2023年第4期10卷 1626-1640页

作者： Fanchun Zeng chunrong zhao Rujie Wang Lingyan Ren Hao Qiu Zhi Zou Haibin Ding Zhongyi Sun Jianmei Li Shiwu Dong Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular ImplantsCollege of BioengineeringChongqing UniversityChongqing 400030China Department of Biomedical Materials Science Army Medical UniversityChongqing 400038China Department of Orthopedics Xinqiao HospitalArmy Medical UniversityChongqing 400038China Antenatal Diagnosis Centre Guizhou Provincial People’s HospitalGuiyangGuizhou 550003China Department of Urology Shenzhen University General HospitalShenzhen UniversityShenzhenGuangdong 518055China

More than 50%of prostate cancer(PCa)patients have bone metastasis with osteo-blastic lesions.MiR-18a-5p is associated with the development and metastasis of PCa,but it remains unclear whether it is involved in osteoblastic lesions.We first found that miR-18a-5p was highly expressed in the bone microenvironment of patients with PCa bone metastases.To address how miR-18a-5p affects PCa osteoblastic lesions,antagonizing miR-18a-5p in PCa cells or pre-osteoblasts inhibited osteoblast differentiation in vitro.Moreover,injection of PCa cells with miR-18a-5p inhibition improved bone biomechanical properties and bone mineral mass in vivo.Furthermore,miR-18a-5p was transferred to osteoblasts by exosomes derived from PCa cells and targeted the Hist1h2bc gene,resulting in Ctnnb1 up-regulation in the Wnt/β-catenin signaling pathway.Translationally,antagomir-18a-5p significantly improved bone biomechanical properties and alleviated sclerotic lesions from osteoblastic me-tastases in BALB/c nude mice.These data suggest that inhibition of exosome-delivered miR-18a-5p ameliorates PCa-induced osteoblastic lesions.

关键词： Bone metastasis MiR-18a-5p Osteoblastic lesions Prostate cancer Wnt/β-catenin pathway

Thermal characteristics of melting of a phase change material enhanced by a stainless-steel 304 periodic structure

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Energy Storage and Saving 2023年第2期2卷 442-448页

作者： chunrong zhao Michael Opolot Patrick Keane Ji Wang Ming Liu Frank Bruno Simone Mancin Kamel Hooman School of Mechanical and Mining Engineering The University of QueenslandBrisbane4072Australia School of Aerospace Mechanical and Mechatronic EngineeringThe University of SydneyNSW2006Australia Future Industries Institute University of South AustraliaMawson Lakes5095Australia Department of Management and Engineering University of PadovaVicenza36100Italy Department of Process and Energy Delft University of TechnologyLeeghwaterstraat 392628 CB DelftThe Netherlands

In this work,melting of a high-temperature inorganic phase change material(PCM)eutectic(with a melting point of 569℃)within a vertical cylindrical tank has been experimentally investigated.To promote the heat transfer rate,a periodic structure that is constructed by a commercial SS-304 mesh screen has been considered and immersed into the PCM tank.Thermal characteristics of the PCM-periodic structure tank under different initial temperatures(450,490 and 546℃)and wall temperatures(620,640,660,680 and 700℃),are then investigated and reported.The presented experimental data can facilitate practical engineers to find the best operating condition of similar PCM tanks;meanwhile,it can also be employed for the investigation of thermal response of transient heat conduction before melting starts.

关键词： Melting enhancement Thermal characteristics Experiment

Long non-coding RNA HCAR promotes endochondral bone repair by upregulating VEGF and MMP13 in hypertrophic chondrocyte through sponging miR-15b-5p

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Genes & Diseases 2022年第2期9卷 456-465页

作者： Yun Bai Xiaoshan Gong Rui Dong Zhen Cao Ce Dou Chuan Liu Jianmei Li Fei Kang Jingjin Dai chunrong zhao Zhansone Tian Jiulin Tan Qijie Dai Shiwu Dong Department of Biomedical Materials Science School of Biomedical EngineeringThird Military Medical UniversityChongqing 400038PR China Department of Orthopedics Southwest HospitalThird Military Medical UniversityChongqing 400038PR China State Key Laboratory of Trauma Burns and Combined InjuryThird Military Medical UniversityChongqing 400038PR China Institute of Trauma Orthopedics Army General Hospital of PLABeijing 100700PR China

Endochondral bone formation is an important route for bone repair.Although emerging evidence has revealed the functions of long non-coding RNAs(lncRNAs)in bone and cartilage development,the effect of lncRNAs in endochondral bone repair is still largely unknown.Here,we identified a lncRNA,named Hypertrophic Chondrocyte Angiogenesis-related lncRNA(HCAR),and proved it to promote the endochondral bone repair by upregulating the expression of matrix metallopeptidase 13(Mmp13)and vascular endothelial growth factorα(Vegfa)in hypertrophic chondrocytes.Lnc-HCAR knockdown in hypertrophic chondrocytes restrained the cartilage matrix remodeling and decrease the CD31hiEmcnhi vessels number in a bone repair model.Mechanistically,we proved that lnc-HCAR was mainly enriched in the cytoplasm using fluorescence in situ hybridization(FISH)assay,and it acted as a molecular sponge for miR-15b-5p.Further,in hypertrophic chondrocytes,lnc-HCAR competitively bound to miR-15b-5p to increase Vegfa and Mmp13 expression.Our results proved that lncRNA is deeply involved in endochondral bone repair,which will provide a new theoretical basis for future strategies for promoting fracture healing.

关键词： Bone marrow mesenchymal stem cells Bone repair Chondrocyte Enchondral bone repair Long non coding RNA

Physiological Processes Contributing to the Difference in Grain Amino Acid Content Between Two Hybrid Rice(Oryza sativa L.)Cultivars

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Phyton-International Journal of Experimental Botany 2019年第2期88卷 101-107页

作者： Min Huang Hengdong Zhang chunrong zhao Guanghui Chen Yingbin Zou Crop and Environment Research Center for Human Health College of AgronomyHunan Agricultural UniversityChangsha410128China.

Improving grain amino acid content of rice(Oryza sativa L.)is essential for the health of consumers.This study was conducted to identify the physiological processes that contribute to the higher grain amino acid content in hybrid rice cultivar Lingliangyou 268 compared to Luliangyou 996.The results showed that total amino acid content in grains was 9%higher in Lingliangyou 268 than in Luliangyou 996.There was no significant difference in grain nitrogen(N)content between Lingliangyou 268 and Luliangyou 996,while ratio of amino acid to N was 6%higher in Lingliangyou 268 compared to Luliangyou 996.A total of 16 differentially expressed proteins related to amino acid metabolism(e.g.,erythronate-4-phosphate dehydrogenase domain containing protein)were identified in grains between Lingliangyou 268 and Luliangyou 996.The identified proteins were involved in 10 molecular functions.Six of the 10 defined functions were related to binding(heterocyclic compound binding,nucleoside phosphate binding,nucleotide binding,organic cyclic compound binding,protein binding,and small molecule binding)and the other 4 defined functions were catalytic activity,enzyme regulator activity,hydrolase activity,and transferase activity.These results indicate that the higher grain amino acid content in Lingliangyou 268 compared to Luliangyou 996 is attributed to increased efficiency of converting N to amino acid that results from altered expression of proteins related to amino acid metabolism.

关键词： Amino acid content grain quality protein expression protein function hybrid rice

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GFRP材料光伏基础支架的力学仿真分析

北京工业职业技术学院学报 2024年第3期23卷 6-10页

作者：赵春荣崔炜北京工业职业技术学院建筑与测绘工程学院北京100042 中国水利水电科学研究院北京100038

针对淤泥地基固有缺陷及光伏支架受力特点,提出一种新型玻璃纤维增强复合材料(GFRP)浅埋圆形扩展式基础加空心立柱支架的结构设计方案,并进行了力学仿真分析。仿真结果显示:该光伏基础支架结构在极端瞬时风荷载作用下能保障承载安全,地... 详细信息

针对淤泥地基固有缺陷及光伏支架受力特点,提出一种新型玻璃纤维增强复合材料(GFRP)浅埋圆形扩展式基础加空心立柱支架的结构设计方案,并进行了力学仿真分析。仿真结果显示:该光伏基础支架结构在极端瞬时风荷载作用下能保障承载安全,地基无大面积破坏;实测风压循环加载条件下,结构未触变破坏,位移和应力水平维持在可控范围。为降低材料成本同时控制结构变形,建议增加基础内部肋板或支撑、扩大抗风方向尺寸以提升结构刚度;针对圆形扩展式基础埋置施工,提出采用带护筒的大直径旋挖工艺的可行性建议。该研究为光伏基础支架新结构开发提供了理论支撑。

关键词：光伏基础支架玻璃纤维增强复合材料力学仿真

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丙二酸的生物合成及其发酵优化

食品与发酵工业 2024年第1期50卷 44-51页

作者：凌春榕杨小雁耿嘉宝毛银赵运英邓禹江南大学粮食发酵与食品生物制造国家工程研究中心江苏无锡214122 江南大学江苏省生物活性产品加工工程研究中心江苏无锡214122 山东渤海实业集团有限公司山东省油脂油料精深加工技术重点实验室山东滨州256599

丙二酸作为一种重要的二元羧酸,广泛应用于多种领域。但目前由于生物法合成丙二酸产量较低,不适合进行工业化生产。为提高丙二酸的生物法合成能力,该文以大肠杆菌BL21(DE3)为底盘细胞,通过过量表达ppc、aspA、panD、sdhC、pa0132及yneI... 详细信息

丙二酸作为一种重要的二元羧酸,广泛应用于多种领域。但目前由于生物法合成丙二酸产量较低,不适合进行工业化生产。为提高丙二酸的生物法合成能力,该文以大肠杆菌BL21(DE3)为底盘细胞,通过过量表达ppc、aspA、panD、sdhC、pa0132及yneI基因,构建了丙二酸生物合成工程菌BL21(PPP)。在摇瓶条件下,对该菌株的发酵条件进行了优化,丙二酸合成量达0.48 g/L,较优化前提高了1倍。同时,该文探究了外源添加生物素及富马酸对丙二酸合成的影响,在添加75μg/L生物素的发酵条件下,丙二酸的产量较对照组提高了32.59%;在添加终质量浓度为8 g/L的富马酸的条件下,该菌株可合成1.2 g/L丙二酸。在5 L发酵罐中,该菌株最高可以合成12.42 g/L的丙二酸。该文实现了丙二酸在大肠杆菌BL21(DE3)中的生物合成,并通过对发酵条件的优化进一步提高了丙二酸的产量,为更进一步提高丙二酸产量奠定了基础。

关键词：大肠杆菌丙二酸富马酸生物合成发酵优化

Dynamic Study on Tourism Carbon Footprint and Carbon Carrying Capacity in Sichuan Province

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Journal of Geoscience and Environment Protection 2019年第7期7卷 14-24页

作者： Yu Ren chunrong zhao Juanlin Fu School of Civil Engineering and Architecture Southwest University of Science and Technology Mianyang China

Tourism carbon emission is one of the important factors affecting ecological environment. In order to clarify the trend and changing characteristics of tourism carbon emissions, academia has carried out quantitative research on tourism carbon footprint. Based on the theory of carbon footprint, this study analyzes the tourism carbon emissions in Sichuan Province from 2004 to 2018 through three indexes: tourism carbon footprint, carbon carrying capacity and net carbon footprint. The results show that the carbon footprint of tourism in Sichuan Province shows an increasing trend. During the fifteen years, it increases by 20.2 times from 427.59 million tons to 9505.95 million tons. The carbon carrying capacity of tourism is increasing year by year except in 2008. Sichuan Province was in a carbon surplus before 2012. The carbon carrying capacity is greater than the carbon footprint, which is friendly to the ecological environment. After 2012, Sichuan Province is in a state of carbon deficit, and the ecological pressure is increasing, which is not conducive to human survival.

关键词： Tourism Carbon Footprint Tourism Carbon Carrying Capacity Net Carbon Footprint Tourism Sichuan Province

Combining immune checkpoint blockade with ATP-based immunogenic cell death amplifier for cancer chemo-immunotherapy

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Acta Pharmaceutica Sinica B 2022年第9期12卷 3694-3709页

作者： Jiulong Zhang Xiaoyan Sun Xiufeng zhao chunrong Yang Menghao Shi Benzhuo Zhang Haiyang Hu Mingxi Qiao Dawei Chen Xiuli zhao School of Pharmacy Shenyang Pharmaceutical UniversityShenyang 110016China Department of Oncology Affiliated Hongqi Hospital of Mudanjiang Medical CollegeMudanjiang 157011China Department of Pharmacy Shantou University Medical CollegeShantou 515041China Department of Neurology The Second Affiliated Hospital of Mudanjiang Medical CollegeMudanjiang 157009China

Amplifying“eat me signal”during tumor immunogenic cell death(ICD)cascade is crucial for tumor immunotherapy.Inspired by the indispensable role of adenosine triphosphate(ATP,a necessary“eat me signal”for ICD),a versatile ICD amplifier was developed for chemotherapy-sensitized immunotherapy.Doxorubicin(DOX),ATP and ferrous ions(Fe^(2+))were co-assembled into nanosized amplifier(ADO-Fe)throughπ‒πstacking and coordination effect.Meanwhile,phenylboric acid-polyethylene glycol-phenylboric acid(PBA-PEG-PBA)was modified on the surface of ADO-Fe(denoted as PADO-Fe)by the virtue of d-ribose unit of ATP.PADO-Fe could display active targetability against tumor cells via sialic acid/PBA interaction.In acidic microenvironment,PBA-PEG-PBA would dissociate from amplifier.Moreover,high H_(2)O_(2)concentration would induce hydroxyl radical(·OH)and oxygen(O_(2))generation through Fenton reaction by Fe^(2+).DOX and ATP would be released from the amplifier,which could induce ICD effect and“ICD adjuvant”to amplify this process.Together with programmed death ligands 1(PD-L1)checkpoint blockade immunotherapy,PADO-Fe could not only activate immune response against primary tumor,but also strong abscopal effect against distant tumor.Our simple and multifunctional ICD amplifier opens a new window for enhancing ICD effect and immune checkpoint blockade therapy.

关键词： Immunogenic cell death amplifier ATP Fenton reaction Immune checkpoint blockade Coordination polymer nanoparticles pH/ROS sensitive Phenylboric acid

江西省宜丰县白市化山锂矿床地质特征及矿床成因探讨

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东华理工大学学报（自然科学版） 2024年第2期47卷 164-174页

作者：刘丛赵立民潘春蓉张勇戴浩橦徐欣宇潘家永东华理工大学核资源与环境国家重点实验室江西南昌330013 江西省地质调查勘查院矿产勘查所江西南昌330038 东华理工大学战略性矿产成矿理论与深部预测江西省重点实验室江西南昌330013

白市化山锂矿床为宜丰地区近年新发现的超大型花岗岩型锂矿床,估算Li_(2)O资源量超100万t,平均品位为0.39%。为研究围岩蚀变及探讨矿床成因,在野外地质调查的基础上,开展了岩相学观察和Li_(2)O含量分析。结果表明,白市化山锂矿床成矿岩... 详细信息

白市化山锂矿床为宜丰地区近年新发现的超大型花岗岩型锂矿床,估算Li_(2)O资源量超100万t,平均品位为0.39%。为研究围岩蚀变及探讨矿床成因,在野外地质调查的基础上,开展了岩相学观察和Li_(2)O含量分析。结果表明,白市化山锂矿床成矿岩体为白水洞白云母(二长)花岗岩,与成矿关系密切的蚀变为钠长石化、白(锂)云母化。该矿床围岩蚀变具有明显的分带性,平面上由南西往北东、垂向上自上而下可以划分为强、中、弱钠长石化锂云母化白云母(二长)花岗岩三个蚀变矿化带。该矿床是岩浆高度分异演化使锂等稀有金属逐步富集形成,岩浆期后热液蚀变作用促使Li富集在花岗岩顶部、边部及其外接触带浅色云母中。

关键词：花岗岩型锂矿围岩蚀变白市化山江西宜丰