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Potential application of FoldX force field based protein modeling in zinc finger nucleases design

Science China(Life Sciences) 2011年第5期54卷 442-449页

作者： HE ZuYong MEI Gui zhao chunpeng CHEN YaoSheng Slate Key Laboratory of Biocontrol School of Life Sciences Sun Yat-Sen University Guangzhou 510006 China Institute of Animal Sciences Guangdong Academy of Agricultural Sciences Guangzhou 510640 China College of Animal Science and Technology South China Agricultural University Guangzhou 510640 China

Engineered sequence-specific zinc finger nucleases (ZFNs) make the highly efficient modification of eukaryotic genomes ***,most current strategies for developing zinc finger nucleases with customized sequence specificities require the construction of numerous tandem arrays of zinc finger proteins (ZFPs),and subsequent largescale in vitro validation of their DNA binding affinities and specificities via bacterial *** labor and expertise required in this complex process limits the broad adoption of ZFN *** effective computational assisted design strategy will lower the complexity of the production of a pair of functional *** we used the FoldX force field to build 3D models of 420 ZFP-DNA complexes based on zinc finger arrays developed by the Zinc Finger Consortium using OPEN (oligomerized pool engineering).Using nonlinear and linear regression analysis,we found that the calculated protein-DNA binding energy in a modeled ZFP-DNA complex strongly correlates to the failure rate of the zinc finger array to show significant ZFN activity in human *** our models,less than 5% of the three-finger arrays with calculated protein-DNA binding energies lower than 13.132 kcal mol 1 fail to form active ZFNs in human *** contrast,for arrays with calculated protein-DNA binding energies higher than 5 kcal mol 1,as many as 40% lacked ZFN activity in human ***,we suggest that the FoldX force field can be useful in reducing the failure rate and increasing efficiency in the design of ZFNs.

关键词： zinc finger nuclease FoldX force field protein mutation modeling

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Effect of high-multiple water injection on rock pore structure and oil displacement efficiency

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Energy Geoscience 2024年第1期5卷 234-238页

作者： Xiao Lei chunpeng zhao Qiaoliang Zhang Panrong Wang Runfu Xiong Zhanjiang Branch Company CNOOCZhanjiang524057GuangdongChina SINOPEC Petroleum Exploration and Production Research Institute Beijing100083China

Experimental methods,including mercury pressure,nuclear magnetic resonance(NMR)and core(wateroil)displacement,are used to examine the effects of high-multiple water injection(*** injection with high injected pore volume)on rock properties,pore structure and oil displacement efficiency of an oilfield in the western South China *** results show an increase in the permeability of rocks along with particle migration,an increase in the pore volume and the average pore throat radius,and enhanced heterogeneity after high-multiple water *** with normal water injection methods,a high-multiple water injection is more effective in improving the oil displacement *** degree of recovery increases faster in the early stage due to the expansion of the swept area,and the transition from oil-wet to *** degree of recovery increases less in the late stage due to various factors,including the enhancement of heterogeneity in the *** both the economic aspect and the production limit of water flooding,it is recommended to adopt other technologies to further enhance oil recovery after 300 PV water injection.

关键词： High multiple Water injection Rock permeability Pore structure Oil displacement efficiency

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Experimental and finite element analysis studies of a reduction-force reducing traction method for pelvic fracture surgeries

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Medicine in Novel Technology and Devices 2022年第1期13卷 31-39页

作者： Jixuan Liu Ke Xu chunpeng zhao Gang Zhu Yu Wang Xinbao Wu Wei Tian Key Laboratory of Biomechanics and Mechanobiology(Beihang University) Ministry of EducationBeijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing100083China Beijing Jishuitan Hospital BeijingChina Beijing Rossumrobot Ltd BeijingChina

Pelvic fracture is among the most complicated fractures in traumatic orthopedics,with high mortality and morbidity *** main difficulty associated with the reduction surgery is significant muscle *** then becomes necessary to decrease the reduction force against this strong muscle resistance,for improving surgical ***,we propose a novel traction method for decreasing the reduction force during pelvic reduction,and investigate the performance of the elastic traction method on decreasing the reduction force using experimental tests and simulation-based *** the experimental results,the reduction force decreased by 59.2%when 10 kg of elastic traction was *** also establish a musculoskeletal model of the pelvic fracture reduction,for analyzing the muscle resistance and the optimal traction force applied in reduction *** elastic traction method can counteract the muscle resistance increase in the non-traction direction owing to its *** conclude that the optimal traction force applied should be in the 10–15 kg range,and recommend adopting a dynamic traction strategy rather than continuous traction in clinical *** traction is very promising for various surgeries that require traction,including pelvic *** significantly reduces force,which can significantly reduce the physical exertion of the operating surgeon,the possibility of additional injuries to the operated patient,and promotes robot-assisted reduction surgeries.

关键词： Reduction force of pelvic fracture Elastic traction Muscle resistance analysis

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Physical simulation of fluid flow and production performance in extra-low permeability porous media

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Petroleum Science 2009年第4期6卷 415-420页

作者： You Yuan Yue Xiang’an Li Mingyi zhao chunpeng Zhang Tao Key Laboratory of Petroleum Engineering Ministry of EducationChina University of PetroleumBeijing 102249China Enhanced Oil Recovery Research Center China University of PetroleumBeijing 102249China Jilin Oil Field PetroChinaSongyuanJilin 138000China Exploration&Production Research Institute SinopecBeijing 100083China Exploration Department Changqing Oil FieldPetroChinaXi’anShaanxi 710018China

For extra-low permeability reservoirs, with a permeability of about 0.3×10?3 μm2, fluid flow and production performance in cores were studied. A long core holder with a multi-location piezometric measurement was specially designed. An artificial long core, about 700 mm long and with a cross section of 45mm×45mm, was used. In the experiment, pressure distribution along the core can be measured in real time. Single phase flow in the core was investigated. Different modes of production in long cores were also simulated including natural depletion, water flooding, and advanced water flooding. Through physical simulation, flow parameters were collected and production characteristics in extra-low permeability cores were studied. From experimental results, it can be seen that fluid flow in extra-low permeability cores is different from that in high permeability cores. Transmission of pressure in extra-low permeability cores is very slow, and it needs a long time for the pressure to become stable. The distribution curve of pressure along the core is nonlinear and the production rate in extra-low permeability reservoirs decreases sharply. The development effects of different production modes in extra-low permeability cores were compared with one another. Among the production modes, advanced water flooding has much potential for effective development of extra-low permeability reservoirs. Natural depletion and conventional water flooding can also be used in early production periods. In addition, the countermeasures and some ideas especially for the potential development of extra-low permeability reservoirs are suggested.

关键词： Extra-low permeability reservoirs physical simulation fluid flow production performance

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The structure-based design of zinc finger nucleases can facilitate genomic editing

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Chinese Science Bulletin 2011年第21期56卷 2300-2300页

作者： ZuYong He Gui Mei chunpeng zhao YaoSheng Chen

A recent study of significant research value carried out at the State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou,

关键词：建模设计核酸酶锌指基因组生命科学学院国家重点实验室编辑基础

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水冷燃料电池空气供给系统建模与控制研究

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太阳能学报 2021年第9期42卷 428-433页

作者：胡佳丽赵春鹏肖铎浙大城市学院杭州310015

针对水冷质子膜燃料电池空气供给系统多参数多变量的控制特性,在一维方向上建立状态空间模型并提出线性与非线性2种控制策略:基于卡尔曼滤波器的线性二次型最优控制与基于拓展状态观测器的二阶滑模控制算法,并通过遗传算法对控制参数进... 详细信息

针对水冷质子膜燃料电池空气供给系统多参数多变量的控制特性,在一维方向上建立状态空间模型并提出线性与非线性2种控制策略:基于卡尔曼滤波器的线性二次型最优控制与基于拓展状态观测器的二阶滑模控制算法,并通过遗传算法对控制参数进行优化。仿真结果表明:2种控制算法均具有较好的控制效果,能在不同负载电流下迅速准确调节过氧比至设定值,调节时间相比传统PID缩短约30%,空压机消耗功率减少约10%。

关键词：燃料电池非线性控制系统卡尔曼滤波遗传算法滑模控制

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高速垂向运动声源声场干涉特性及其可观测性

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声学学报 2023年第6期48卷 1081-1097页

作者：赵春鹏梁国龙邱龙皓付进柳国龙哈尔滨工程大学水声技术全国重点实验室哈尔滨150001 工业和信息化部海洋信息获取与安全工信部重点实验室(哈尔滨工程大学) 哈尔滨150001 哈尔滨工程大学水声工程学院哈尔滨150001

以高速垂向运动声源宽带连续谱噪声为研究对象,基于射线理论分析了声场干涉现象的产生机理,推导了干涉条纹的轨迹方程,讨论了影响干涉结构特性的因素,揭示了声场干涉结构的特性和规律。理论和仿真结果表明:干涉周期与多途信号时延差成... 详细信息

以高速垂向运动声源宽带连续谱噪声为研究对象,基于射线理论分析了声场干涉现象的产生机理,推导了干涉条纹的轨迹方程,讨论了影响干涉结构特性的因素,揭示了声场干涉结构的特性和规律。理论和仿真结果表明:干涉周期与多途信号时延差成反比关系;减小接收深度或增大接收距离可以提高干涉条纹的可观测性;较大的海面起伏易使干涉条纹形成断点,可观测性降低。湖试数据分析结果表明,干涉条纹主要由直达声-海面反射声干涉条纹构成,干涉条纹宽度和干涉周期随水平距离的增大而增大。该结果可为高速垂向运动声源运动状态分析、环境参数反演等提供参考。

关键词：声场干涉特性干涉条纹水下动目标观测浅海波导随机海面起伏

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局部封闭为主治疗腰椎骨裂伤和软骨终板炎8例

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中国继续医学教育 2015年第2期7卷 88-89页

作者：赵春朋鞍山市海城正骨医院 114200

目的：探讨分析局部封闭为主治疗腰椎骨裂伤和软骨终板炎的疗效。方法选取我院2013年1月-2013年12月间收治的8例腰椎骨裂伤和软骨终板炎患者，给予局部封闭为主治疗，观察疗效。结果本组8例患者，3例痊愈，5例显效，总有效率为100﹪（8/... 详细信息

目的：探讨分析局部封闭为主治疗腰椎骨裂伤和软骨终板炎的疗效。方法选取我院2013年1月-2013年12月间收治的8例腰椎骨裂伤和软骨终板炎患者，给予局部封闭为主治疗，观察疗效。结果本组8例患者，3例痊愈，5例显效，总有效率为100﹪（8/8）。结论局部封闭为主治疗腰椎骨裂伤和软骨终板炎的临床疗效显著。

关键词：局部封闭腰椎骨裂伤软骨终板炎

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活血化瘀汤联合按摩治疗骨伤后期肢体肿胀55例

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中国中医药现代远程教育 2019年第13期17卷 65-66页

作者：赵春朋辽宁省海城市正骨医院创伤骨科(二)疗区

目的探究活血化瘀汤联合中医按摩治疗骨伤后期肢体肿胀的效果。方法选取我院2017年1月—2018年6月骨伤后期肢体肿胀患者110例,随机分为行常规西医治疗的对照组和行活血化瘀汤联合中医按摩治疗的试验组,各55例。对比疗效。结果试验组临... 详细信息

目的探究活血化瘀汤联合中医按摩治疗骨伤后期肢体肿胀的效果。方法选取我院2017年1月—2018年6月骨伤后期肢体肿胀患者110例,随机分为行常规西医治疗的对照组和行活血化瘀汤联合中医按摩治疗的试验组,各55例。对比疗效。结果试验组临床治疗总有效率明显高于对照组,P<0.05;试验组肿胀消除时间、住院时间及肢体疼痛评分均小于对照组,P<0.05;2组骨伤后期肢体肿胀患者在治疗过程中未出现明显肝功能损害等不良反应。结论活血化瘀汤联合中医按摩治疗骨伤后期肢体肿胀患者疗效显著,能有效缓解患者疼痛,缩短治疗时间且未见不良反应发生。

关键词：活血化瘀汤按摩骨伤肿胀

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四川盆地东南部下志留统龙马溪组一段页岩微—纳米观地质特征

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天然气工业 2017年第9期37卷 23-30页

作者：龙胜祥彭勇民刘华赵春鹏赵剑华吴靖俞凌杰中国石化页岩油气勘探开发重点实验室中国石化石油勘探开发研究院中国石化无锡石油地质研究所

下志留统龙马溪组一段是四川盆地东南部页岩气勘探开发的目标层系之一,以往对其地质特征的研究多停留在单方面、定性层面。为此,在钻井岩心观察与描述的基础上,应用微—纳米分析技术和模拟技术,对该层系的微—纳米观地质特征进行了分析... 详细信息

下志留统龙马溪组一段是四川盆地东南部页岩气勘探开发的目标层系之一,以往对其地质特征的研究多停留在单方面、定性层面。为此,在钻井岩心观察与描述的基础上,应用微—纳米分析技术和模拟技术,对该层系的微—纳米观地质特征进行了分析。结果显示:(1)页岩矿物组成横向发育稳定,但纵向变化明显,龙一1亚段以灰黑色富有机质硅质页岩为主,有机质含量高,石英含量高且主要为有机成因石英;(2)龙一2亚段以灰黑色—黑色含粉砂质页岩及粉砂质页岩为主,较之于龙一1亚段,其砂质含量明显增多,TOC和石英含量则相对较低;(3)龙一3亚段主要为黑灰色黏土质页岩,硅质矿物含量较低,有机质常富集在黏土矿物间呈断续顺层分布;(4)该区页岩主要发育有机质孔隙、黏土矿物孔隙、脆性矿物孔隙和裂缝(含页理缝)等4种储集空间类型,其中有机质孔隙十分发育,多数为纳米级,是页岩气赋存的主要储集空间;(5)龙一_1亚段属于IUPC分类方案中的中孔,孔隙度和渗透率均较高,连通性中—差,喉道直径变小,向上到龙一_2、龙一_3亚段则以IUPC分类方案的大孔为主,但孔隙度降低、连通性变差;(6)该区页岩孔缝细小,孔隙表面积大,对页岩气具有很强的吸附能力,吸附气量随压力增加而增加,页岩气在低速渗流时呈现滑脱效应,渗透率和孔隙压力越小,滑脱效应越明显。

关键词：四川盆地页岩微—纳米观特征有机质孔隙微纳米孔喉吸附能力低速渗流滑脱效应

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