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Expression of estrogen receptor alpha,nerve growth factor,interleukin-2,and androgen receptor in the cerebellum of ovariectomized rats following soybean isoflavone treatment

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Neural Regeneration Research 2009年第12期4卷 965-972页

作者： Zhengli Chen chunmei zhu Qihui Luo Kangcheng Pan Anchun Cheng College of Veterinary Medicine Sichuan Agricultural University Ya'an 625014 Sichuan Province China Experimental Animal Engineering Center Sichuan Agricultural University Ya'an 625014 Sichuan Province China

BACKGROUND： Studies have shown that estrogen receptor alpha （ERα）, nerve growth factor （NGF）, interleukin-2 （IL-2）, and androgen receptor （AR） expression in the cerebellum decreases when estrogen levels decrease in vivo. Soybean isoflavone, a type of non-steroid estrogen with similar molecular structure and function to estradiol, exhibits estrogen-like characteristics. OBJECTIVE： To investigate the effects of various doses of soybean isoflavone on expression of ERa, NGF, IL-2, and AR in the cerebellum of ovariectomized rat, and to determine whether there is a dose-dependent ***, TIME AND SETTING： Controlled trial at the cellular and molecular level. The study was performed at the Experimental Animal Engineering Center, College of Veterinary Medicine, Sichuan Agricultural University from July 2006 to May 2008. MATERIALS： Soybean isoflavone, comprised of daidzin, genistein and isoflavone, was provided by Taiyuan Yuantai Biochemical Industry, China. The ERα, NGF, IL-2, and AR in situ hybridization kit, rabbit anti-rat ERa, NGF, IL-2, and AR monoclonal antibodies, and SABC kit were purchased from Wuhan Boster Biological Technology, China. METHODS： A total of 50 female, Sprague Dawley rats, aged 3 months, were randomly assigned to 5 groups, with 10 animals in each group. With the exception of the sham-operation group （abdominal cavity opening alone）, all rats underwent bilateral ovariectomy. At 14 days after surgery, rats in the high-, middle-, and low-dose soybean isoflavone groups were subcutaneously injected with 1.5, 1.0, and 0.5 mg/kg soybean isoflavone, respectively, every 2 days for 6 consecutive weeks. Rats in the sham-operation and ovariectomized groups were subcutaneously injected with absolute alcohol （0.5 mL/kg）. MAIN OUTCOME MEASURES： Expression levels and distribution of ERα, NGF, IL-2, and AR in the cerebellum were detected by immunohistochemistry and in situ hybridization. RESULTS： Compared with the sham-operation group, immunoreactive products

关键词： soybean isoflavone estrogen receptor alpha nerve growth factor interleukin-2 androgen receptor cerebellum

Synthesis and characterization of superhydrophobic magnesium oxysulfate whiskers

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Particuology 2023年第7期78卷 111-121页

作者： Zhenying Yao Lu Gong chunmei zhu Xin Lian Bai He Shenghui Tian Bo Yui School of Chemistry and Chemical Engineering Chongqing University of Science and TechnologyChongqing401331China Provincial and Ministerial Co-Constructive of Collaborative Innovation Center for MSw Comprehensive Utilization School of Metallurgy and Materials EngineeringChongqing University of Science and TechnologyChongqing401331China

Superhydrophobic materials have attracted much attention for their special *** this *** oxysulfate(MOS)whiskers were surface modified by vinyltrimethoxysilane(VTMS)and prepared as superhydrophobic materials,which are expected to be widely used in self-cleaning,corro-sion prevention,and oil-water *** factors of silane concentration,hydrolysis time,reaction temperature,and reaction time were *** superhydrophobic Mos whiskers were *** and XRD turned out that there were no apparent changes in the morphology and crystalli-zation behavior of whiskers before and after modification,while the surface was uniformly coated with a layer of non-crystal material,and the surface of the whiskers employed a chemical bond Si-O-Mg covalently *** thermogravimetric analysis ultimately demonstrated that surface modification was beneficial to the improvement of the thermal stability of MoS *** MoS whiskers showed good compatibility with organic solvents through oil-water separation experiments,and demonstrated excellent self-cleaning *** methodology for the surface treatment of Mos whiskers to prepare superhydrophobic whiskers in this work may be extended for other whiskers or fillers,which may be promising for the preparation of superhydrophobic materials.

关键词： Magnesium oxysulfate whiskers Surface modification Vinyltrimethoxysilane Superhydrophobicity

Phosphorylation of OsFD1 by OsCIPK3 promotes the formation of RFT1-containing florigen activation complex for long-day flowering in rice

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Molecular Plant 2021年第7期14卷 1135-1148页

作者： Qiang Peng chunmei zhu Tao Liu Shuo Zhang Shijing Feng Changyin Wu National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070China Guizhou Rice Research Institute Guizhou Academy of Agricultural SciencesGuiyang 550006China

Heading date is a critical trait that determines the regional adaptability and grain productivity of many *** rice is a facultative short-day plant,its domestication led to the Ghd7-Ehd1-Hd3a/RFT1 pathway for adaptation to long-day conditions(LDs).The formation of the"florigen activation complex"(FAC)containing florigen Hd3a has been ***,the molecular composition of the FAC that contains RFT1 for long-day flowering is *** show here that RFT1 forms a ternary FAC with 14-3-3 proteins and OsFD1 to promote flowering under *** identified a calcineurin B-like-interacting protein kinase,OsCIPK3,which directly interacts with and phosphorylates OsFD1,thereby facilitating the localization of the FAC to the *** in OsCIPK3 results in a late heading date under LDs but a normal heading date under short-day ***,our results suggest that OsCIPK3 phosphorylates OsFD1 to promote RFT1-containing FAC formation and consequently induce flowering in rice under LDs.

关键词： rice heading date florigen activation complex OsCIPK3 phosphorylation

Defects-rich MgFe LDH:A high-capacity adsorbent for methyl orange wastewater

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Chinese Journal of Chemical Engineering 2024年第10期74卷 63-73页

作者： chunmei zhu Liting Zhang Chenyuan Cui Xin Lian Long Qu Bai He Guoyuan Yuan Jian Feng Siwei Xiang Bo Yu School of Chemistry and Chemical Engineering Chongqing University of Science and TechnologyChongqing 401331China Research Center of Smart Environmental Analysis Chongqing Institute of Green and Intelligent TechnologyChinese Academy of SciencesChongqing 400714China

Dye pollution is a common pollutant in wastewater that poses a serious threat to human *** double hydroxide(LDH)is a commonly used adsorbent for dye ***,its adsorption efficiency is significantly limited by the limited adsorption active sites of the *** this paper,a defects-rich MgFe LDH adsorbent for anionic dye wastewater was synthesized by a simple hydrothermal method and alkaline *** analytical techniques,such as XRD,FT-IR,SEM,TEM,XPS,and N2 adsorption-desorption isotherm,were used to verify the chemical composition and surface characteristics of the materials,and the effects of pH,temperature,and contact time on the adsorption effect of methyl orange and the adsorption mechanism were *** etching of Al and Zn in the laminate generated defects that expose unsaturated coordination centers and create abundant adsorption sites,which can electrostatically attract and coordinate with dye *** 25℃,the adsorption capacity of MgFe LDH with Al etched and MgFe LDH with Zn etched for methyl orange dye reached 1722 mg·g^(-1 ) and 1685 mg·g^(-1 ),respectively,much higher than that of MgFe LDH(544 mg·g^(-1 )).This work provides a promising method for the removal of dye wastewater by adsorption and a new idea for the design and development of high-performance dye wastewater adsorbents.

关键词： MgFe LDH Methyl orange Alkaline etching Defects Adsorption

Application of Human Adenovirus Genotyping by Phylogenetic Analysis in an Outbreak to Identify Nosocomial Infection

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Virologica Sinica 2021年第3期36卷 393-401页

作者： Chuanyu Yang chunmei zhu Yuan Qian Jie Deng Baoyuan Zhang Runan zhu Fang Wang Yu Sun Dongmei Chen Qi Guo Yutong Zhou Lei Yu Ling Cao Linqing Zhao Laboratory of Virology Beijing Key Laboratory of Etiology of Viral Diseases in ChildrenCapital Institute of PediatricsBeijing 100020China Department of Respiratory Medicine Affiliated Children's HospitalCapital Institute of PediatricsBeijing 100020China Hospital Infection-Control Department Affiliated Children’s HospitalCapital Institute of PediatricsBeijing 100020China

Nosocomial infections are common in pediatric patients and can be fatal in infants and immunocompromised patients. In September 2018, a high positive rate of human adenovirus HAdV was occurred among hospitalized children in the Children's Hospital Affiliated to the Capital Institute of Paediatrics in Beijing. To investigate whether this outbreak of HAdV was related to nosocomial infections or the result of community infections, we collected respiratory specimens from patients with acute respiratory infections in a respiratory ward during June to December 2018, and screened for respiratory viruses. Among 1,840 cases included, 95(5.2%, 95/1840) were positive for HAdV and 81 were genotyped based on phylogenetic analysis, including seven as HAdV-1(8.6%), 30 HAdV-3(37.0%), two HAdV-6(2.5%), and 42 HAdV-7(51.9%). More HAdV-positive samples were collected in August(4.7%, 12/255), September(15.0%, 41/274) and October(6.9%, 17/247), with a peak in September 2018. By combining the results of HAdV phylogenetic analysis with clinical data of patients, there were 77 cases(4.2%, 77/1840;81.1%, 77/95) excluded from nosocomial infections, eight cases representing possible infections transmitted by visitors or attending parents, three cases without sequences that might have been due to infection transmitted by roommates positive for HAdV, one case of a roommate without an HAdV sequence, and six cases that shared highly homologous sequences with those of their roommates, for which nosocomial infections might be considered. In conclusion, genotyping of HAdVs based on phylogenetic analysis combined with clinical information provides a powerful method to distinguish nosocomial infections from community acquired infection, especially when tracing the origins of nosocomial infections.

关键词： Acute respiratory tract infection Human adenovirus(HAdV) Phylogenetic analysis Nosocomial infection

HAF1 mediates circadian accumulation of Os ELF3 in determining the heading date of rice under long-day conditions

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HAF1 mediates circadian accumulation of Os ELF3 in determini...

Mutations in Caprine DGAT1 and STAT5A Genes were Associated with Milk Production Traits

2016年全国植物生物学大会

作者： chunmei zhu Qiang Peng Debao Fu Dongxia zhuang Changyin Wu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University

Plant circadian clock has been provided to be involved in multiple physiology and metabolism process by monitoring external environment changes,including photoperiodic flowering regulation. Our previous study showed that Os ELF3 acts as a floral activator in the long-day photoperiodic pathway via its crosstalk with the circadian clock in rice. However,post-transcription regulation of Os ELF3 is still unclear. In this study,we showed that HAF1,an E3 ubiquitin ligase,physically interact with Os ELF3 in vivo and in vitro. C-terminal domain of Os ELF3 was identified as the functional motif for mediating interaction with HAF1,as well as formation of homodimer by itself. Further protein degradation assay indicated that Os ELF3 is the direct substrate of HAF1 for ubiquitination in rice. Genetically,the oself3 haf1 double mutant headed as late as oself3 under long-day conditions. Previous investigation revealed that Os ELF3 plays a crucial role in photoperiodic response to determine rice regional adaptability. We proposed that HAF1 plays a pivotal role in heading date modulation by ubiquitination of Os ELF3 under long-day conditions.

关键词： circadian clock photoperiodic flowering ubiquitination rice

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Engineering（科研） 2012年第10期4卷 30-34页

作者： Xiaopeng An Jinxing Hou Haibo Zhao chunmei zhu Quanmei Yan Yuxuan Song Jiangang Wang Binyun Cao College of Animal Science and Technology Northwest A&F University Yangling Shaanxi P.R. China

In this study, polymorphisms of the DGAT1 andSTAT5A genes were detected in 528 individuals from Xinong Saanen and Guanzhong goat breeds by PCR-RFLP, PCR-SSCP and DNA sequencing methods. Three allelic variants were identified: DQ380250: g.407_408insC, AJ237937: g.6798C>T and g.6852C>T in both breeds. At g.407_408insC locus, the frequencies of C1 allele were 0.79–0.85, and frequencies of C2 allele were 0.21–0.15. At g.6852C>T locus, frequencies of C3 allele were 0.70–0.72, and frequencies of T3 allele were 0.30–0.28. Compared with goats with C1C1 and C3C3, those with C1C2 and C3T3 genotypes had significant effects on milk yield and fat percentage (P<0.05), respectively. The result showed that does with C1C1C3T3 and C1C2C3T3 had higher milk yield than those with C1C2C3C3 (P < 0.05). In addition, the combined effect of C1C2C3C3 on milk fat percentage was the highest in comparison with other combination genotypes (P<0.05).

关键词： Dairy Goat Milk Production Traits Fat Percentage Pedigree

互联网新常态下高校“思政课”青年教师职业素质的提升

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职业技术教育 2015年第17期36卷 77-79页

作者：祝春梅宋治非绥化学院思想政治理论教研部绥化学院经济管理学院

高校"思政课"承担着培养大学生树立社会主义核心价值观,成为经济社会发展优秀人才的光荣使命。"思政课"青年教师连接着高校思想政治教育工作的现实与未来,他们职业素质、能力的高与低、优与劣直接关系着所培养人才的质量。面对新时期互... 详细信息

高校"思政课"承担着培养大学生树立社会主义核心价值观,成为经济社会发展优秀人才的光荣使命。"思政课"青年教师连接着高校思想政治教育工作的现实与未来,他们职业素质、能力的高与低、优与劣直接关系着所培养人才的质量。面对新时期互联网新常态下带来的挑战,必须从道德素质、文化素质、业务素质和身心素质四个方面来提升高校思政课青年教师的职业素质。

关键词：互联网新常态思政课青年教师职业素质

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一类适应度函数的遗传算法编码

计算机应用 2017年第7期37卷 1972-1976,1998页

作者：朱春媚莫鸿强电子科技大学中山学院机电工程学院广东中山528400 华南理工大学自动化科学与工程学院广州510641

针对在探讨适应度函数的周期性特点与整数编码元数之间的关联特性时,一阶积木块数量对编码性能的评价不一定成立的问题,提出以累积逃脱概率(AEP)作为遗传算法(GA)编码性能的评价指标,对以频率为正整数m的整数次幂的正弦函数为基函数线... 详细信息

针对在探讨适应度函数的周期性特点与整数编码元数之间的关联特性时,一阶积木块数量对编码性能的评价不一定成立的问题,提出以累积逃脱概率(AEP)作为遗传算法(GA)编码性能的评价指标,对以频率为正整数m的整数次幂的正弦函数为基函数线性组合构成的适应度函数编码展开研究。首先给出了该类适应度函数的一般形式和m进制整数编码的含义;然后介绍了AEP的定义,并根据函数特点制定了AEP的计算方法;最后分析比较了该类适应度函数在不同整数编码下的AEP,指出其采用m元整数编码时更容易进化。仿真结果表明,该类适应度函数采用m元整数编码时,其最终优化结果和群体适应度均值的上升时间皆明显优于其他编码,反映了AEP能有效评价编码的性能,并再次验证了对于该类适应度函数m元整数编码优于非m元整数编码的结论。

关键词：编码性能评价遗传算法周期性适应度函数累积逃脱概率