检索结果-南通市图书馆

Toll-like Receptor 4 Deficiency Causes Reduced Exploratory Behavior in Mice Under Approach-Avoidance Conflict

Neuroscience Bulletin 2016年第2期32卷 127-136页

作者： chunlu li Yixiu Yan Jingjing Cheng Gang Xiao Jueqing Gu Luqi Zhang Siyu Yuan Junlu Wang Yi Shen Yu-Dong Zhou Department of Neurobiology and the Collaborative Innovation Center for Brain Science Key Laboratory of Medical Neurobiology of the Ministry of Health of ChinaKey Laboratory of Neurobiology of Zhejiang ProvinceZhejiang University School of Medicine Department of Anesthesiology The First Affiliated Hospital of Wenzhou Medical University Department of Biological Science College of Life SciencesZhejiang University Department of Medicine Zhejiang University School of Medicine

Abnormal approach-avoidance behavior has been linked to deficits in the mesolimbic dopamine（DA）system of the brain. Recently, increasing evidence has indicated that toll-like receptor 4（TLR4）, an important pattern-recognition receptor in the innate immune system,can be directly activated by substances of abuse, resulting in an increase of the extracellular DA level in the nucleus accumbens. We thus hypothesized that TLR4-dependent signaling might regulate approach-avoidance behavior. To test this hypothesis, we compared the novelty-seeking and social interaction behaviors of TLR4-deficient（TLR4^（-/-））and wild-type（WT） mice in an approach-avoidance conflict situation in which the positive motivation to explore a novel object or interact with an unfamiliar mouse was counteracted by the negative motivation to hide in exposed,large spaces. We found that TLR4^（-/-）mice exhibitedreduced novelty-seeking and social interaction in the large open spaces. In less stressful test apparatuses similar in size to the mouse cage, however, TLR4^（-/-）mice performed normally in both novelty-seeking and social interaction tests. The reduced exploratory behaviors under approachavoidance conflict were not due to a high anxiety level or an enhanced fear response in the TLR4^（-/-）mice, as these mice showed normal anxiety and fear responses in the open field and passive avoidance tests, respectively. Importantly,the novelty-seeking behavior in the large open field induced a higher level of c-Fos activation in the nucleus accumbens shell（NAc Sh） in TLR4^（-/-）mice than in WT mice. Partially inactivating the NAc Sh via infusion of GABA receptor agonists restored the novelty-seeking behavior of TLR4^（-/-）mice. These data suggested that TLR4 is crucial for positive motivational behavior under approach-avoidance conflict. TLR4-dependent activation of neurons in the NAc Sh may contribute to this phenomenon.

关键词： Toll-like receptor 4 Novelty-seeking Social interaction Approach-avoidance conflict Nucleus accumbens shell

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Postnatal feeding with high-fat diet induces obesity and precocious puberty in C57BL/6J mouse pups： a novel model of obesity and puberty

引用

Frontiers of Medicine 2017年第2期11卷 266-276页

作者： Rahim Ullah Yan Su Yi Shen chunlu li Xiaoqin Xu Jianwei Zhang Ke Huang Naveed Rauf Yang He Jingjing Cheng Huaping Qin Yu-Dong Zhou Junfen Fu Department of Endocrinology Children's Hospital Zhejiang University School of Medicine Hangzhou 310051 China Department of Neurobiology Institute of Neuroscience and the Collaborative Innovation Center for Brain Science Key Laboratory of Medical Neurobiology of the Ministry of Health of China Zhejiang University School of Medicine Hangzhou 310058 China

Childhood obesity and obesity-related metabolic complications are induced by a high-fat postnatal diet. The lack of a suitable animal model, however, remains a considerable challenge in obesity studies. In the current study, we provided high-fat diet （HFD） to dams during lactation and to pups after weaning. We also developed a novel model of C57BL/6J mouse pups with HFD-induced postnatal obesity. Results showed that feeding with HFD induces fat deposition and obesity in pups. Furthermore, HFD more potently increased the body weight （BW） of male than female pups. HFD-fed female pups were obese, underwent precocious puberty, and showed increased kisspeptin expression in the hypothalamus. However, parental obesity and precocious puberty exerted no synergistic effects on the HFD-induced postnatal weight gain and puberty onset of the pups. Interestingly, some HFD-fed litters with normal BW also exhibited precocious puberty. This finding suggested that diet composition but not BW triggers puberty onset. Our model suggests good construction validity of obesity and precocious puberty. Furthermore, our model can also be used to explore the mutual interactions between diet-induced postnatal childhood obesity and puberty.

关键词： postnatal HFD feeding obesity kisspeptin HPG axis precocious puberty

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Construction of a metastasis-associated gene subtracted cDNA library of human colorectal carcinoma by suppression subtraction hybridization

引用

World Journal of Gastroenterology 2004年第9期10卷 1301-1305页

作者： liliang Yan-QingDing Xinli Guang-ZhiYang JunXiao li-chunlu Jin-HuaZhang DepartmentofPathology theFirstMilitaryMedicalUniversityGuangzhou510515GuangdongProvinceChina DepartmentofTropicMedicine theFirstMilitaryMedicalUniversityGuangzhou510515GuangdongProvinceChina

AIM: To construct a differentially-expressed gene subtracted cDNA library from two colorectal carcinoma (CRC) cell lines with different metastatic phenotypes by suppression subtractive ***: Two cell lines of human CRC from the same patient were used. SW620 cell line showing highly metastatic potential was regarded as tester in the forward subtractive hybridization, while SW480 cell line with lowly metastatic potential was treated as tester in the reverse hybridization. Suppression subtractive hybridization (SSH) was employed to obtain cDNA fragments of differentially expressed genes for the metastasis of CRC. These fragments were ligated with T vectors, screened through the blue-white screening system to establish cDNA ***: After the blue-white screening, 235 white clones were picked out from the positive-going hybridization and 232 from the reverse. PCR results showed that 200-700 bp inserts were seen in 98% and 91% clones from the forward and reverse hybridizations, ***: A subtractive cDNA library of differentially expressed genes specific for metastasis of CRC can be constructed with SSH and T/A cloning techniques.

关键词：结直肠癌肿瘤转移相关基因消减cDNA文库消减杂交法聚合酶链反应

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

雾霾天气下体育运动对呼吸系统的损害程度分析

引用

科技通报 2014年第1期30卷 62-65页

作者：李春璐西安科技大学体育部西安710054

当前的人体呼吸系统性能评估模式缺乏明确物理意义,掩盖或夸大某些雾霾污染物对其的影响。以雾霾天气下进行体育运动的人体呼吸系统变化为分析方向,将雾霾对体育运动人体呼吸系统损害率作为基本的评估方法,对雾霾天气下体育运动人体呼... 详细信息

当前的人体呼吸系统性能评估模式缺乏明确物理意义,掩盖或夸大某些雾霾污染物对其的影响。以雾霾天气下进行体育运动的人体呼吸系统变化为分析方向,将雾霾对体育运动人体呼吸系统损害率作为基本的评估方法,对雾霾天气下体育运动人体呼吸系统性能损害程度进行评估,分析了单项、多项呼吸系统的损害程度评估过程,将人体呼吸系统功能损害程度同天气评估统一起来,确保评估结果具有明确的物理意义,最终获取呼吸系统损害与雾霾天气评估的关系,实现了对雾霾天气下体育运动对呼吸系统的损害程度分析。实验数据结果表明,所提方法分析雾霾天气下体育对呼吸系统损害程度分析具有较高的精确度,取得了令人满意的效果。

关键词：雾霾体育呼吸系统损害污染物

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

氨基酰化酶拆分制备手性蛋氨酸工艺条件研究

引用

化学反应工程与工艺 2006年第1期22卷 88-92页

作者：崔志芳李春露姚善泾山东科技大学化工学院山东青岛266510 浙江大学化学工程与生物工程系浙江杭州310027

以N-乙酰DL-蛋氨酸(N-Ac-DL-Met)为拆分底物,对氨基酰化酶法拆分工艺参数如反应温度、pH、激活剂Co2+浓度以及底物浓度等因素进行了初步考察。结果表明,在反应温度37℃,缓冲体系pH7.5,Co2+浓度为0.5 mmol/L,初始底物N-Ac-DL-Met的浓度为... 详细信息

以N-乙酰DL-蛋氨酸(N-Ac-DL-Met)为拆分底物,对氨基酰化酶法拆分工艺参数如反应温度、pH、激活剂Co2+浓度以及底物浓度等因素进行了初步考察。结果表明,在反应温度37℃,缓冲体系pH7.5,Co2+浓度为0.5 mmol/L,初始底物N-Ac-DL-Met的浓度为0.06 mol/L,氨基酰化酶加入量为5μg/mL的较佳条件下,L-蛋氨酸(L-Met)的产率可达45%;产物L-Met结晶的光学纯度(用对映体过量值%e.e表示)为95.4%。实验中首次采用多组分标准曲线法。

关键词：氨基酰化酶手性拆分蛋氨酸

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

漆酶对微生物溶煤作用的研究

引用

煤炭转化 2007年第2期30卷 14-17页

作者：崔志芳李春露王力山东科技大学化学与环境工程学院山东青岛博士副教授266510

为进一步探讨漆酶在煤炭微生物溶解过程中的作用,考察了溶煤菌种在溶解褐煤过程中漆酶的表达量,以及另外两株产漆酶菌的漆酶表达量与溶煤效果的关系.结果表明:溶煤过程中三株溶煤菌种均未检测到明显的漆酶表达,而两株产漆酶菌尽管具有... 详细信息

为进一步探讨漆酶在煤炭微生物溶解过程中的作用,考察了溶煤菌种在溶解褐煤过程中漆酶的表达量,以及另外两株产漆酶菌的漆酶表达量与溶煤效果的关系.结果表明:溶煤过程中三株溶煤菌种均未检测到明显的漆酶表达,而两株产漆酶菌尽管具有较高的漆酶表达量,却未表现出明显的溶煤活性,即漆酶在微生物溶煤过程中不起关键作用.

关键词：微生物溶解机理褐煤漆酶

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

梅岭地区常见的苔藓植物(Bryophyta)种类

引用

江西农业大学学报 1986年第4期 85-92页

作者：李春鲁江西农业大学园艺系

苔藓植物在我国各省区广布,是一类结构比较简单的高等植物。其适于各种环境、土壤及气候条件,有植物界拓荒者之称。同时,苔藓植物能涵蓄大量水分,对于森林发育、沼泽变迁极有影响。有的还可供燃料、填充料,药棉及供药用等。我国约有二... 详细信息

苔藓植物在我国各省区广布,是一类结构比较简单的高等植物。其适于各种环境、土壤及气候条件,有植物界拓荒者之称。同时,苔藓植物能涵蓄大量水分,对于森林发育、沼泽变迁极有影响。有的还可供燃料、填充料,药棉及供药用等。我国约有二千种左右,常见种类我省各县区皆有分布。现将梅岭地区已鉴定种报道于后。

关键词：中肋叶尖分枝羽状细齿植物体假根 Bryophyta 梅岭

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Additives strategies to improving cycling stability of li metal batteries by modifying electrode electrolyte interphases

引用

Progress in Natural Science:Materials International 2024年第2期34卷 338-344页

作者： Yuan li Pengfei Huang Chunyu Cui Xiaoyuan Ma li Chen Zekun li linhai li Yuhong Tian School of Biological and Chemical Engineering Guangxi University of Science and Technology School of College of Chemistry and Chemical Engineering Hunan University Isky Future Energy Institute

li metal batteries（LMBs） have attracted much attention due to the ultra-high theoretical capacity of the li metal anode（LMA）.However,the uncontrollable dendrites growth and low coulombic efficiency hinder their practical ***,we explore 3,5-difluoropyridine（2F-BD） as a novel electrolyte additive to enable highperformance *** 2F-BD additive participates in and modifies the solvation structure of li ions by reducing the coordinated number of PF6-at the electrode surface,leading to the formation of a liF and li3N-rich solid electrolyte interphase（SEI）.The liF component increases the robustness of SEI and suppresses the formation of li dendrites,while the li3N component facilitates the transportation and reaction kinetics of li *** a result,the li‖li symmetric cell presents a stable cycling performance of up to 500 h at a current density of 1 mA *** coupling with liFePO4cathode,the obtained full cell achieves high specific capacities of 106.06 mA h g-1and 84.98 mA h g-1at 2.55 mA cm-2and 5.10 mA cm-2,respectively,and maintains a high capacity retention of84.0% after 1000 cycles at 0.13 mA cm-2,with an average cycling CE of 99.58%.

关键词： Electrolyte additive li dendrites li metal batteries liF and li3N-Rich electrode-electrolyte interphase liFePO4 cathode

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

楚穗直流孤岛运行方式下电站侧孤岛专用装置研发

引用

水力发电 2015年第10期41卷 60-63页

作者：陈映喜李锐奎李春露华能澜沧江水电股份有限公司小湾水电厂云南大理675702

小湾水电站配套送出工程(楚穗直流)运行方式除联网运行外,还设计了孤岛方式运行。孤岛运行方式与联网运行方式有较大区别,相应的调速、励磁等系统的参数需进行切换,电站侧应设置配合孤岛运行方式的相应装置。根据±800k V楚穗直流孤岛... 详细信息

小湾水电站配套送出工程(楚穗直流)运行方式除联网运行外,还设计了孤岛方式运行。孤岛运行方式与联网运行方式有较大区别,相应的调速、励磁等系统的参数需进行切换,电站侧应设置配合孤岛运行方式的相应装置。根据±800k V楚穗直流孤岛调试试验中的工程经验,总结介绍了电站侧孤岛专用装置与安稳装置之间,孤岛专用装置与站内调速、励磁等系统之间的相互交换信号内容、要求以及接口方案。

关键词：楚穗直流孤岛专用装置交换信号接口方式小湾水电站

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Leaching kinetics of fluorine during the aluminum removal from spent li-ion battery cathode materials

引用

Journal of Environmental Sciences 2024年第4期138卷 312-325页

作者： Shengjie li Jianxin Zhu Research Center for Eco-Environmental Sciences Chinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China

The high content of aluminum(Al)impurity in the recycled cathode powder seriously affects the extraction efficiency of Nickel,Cobalt,Manganese,and lithium resources and the actual commercial value of recycled materials,so Al removal is crucially important to conform to the industrial standard of spent li-ion battery cathode *** this work,we systematically investigated the leaching process and optimum conditions associated with Al removal from the cathode powder materials collected in a wet cathode-powder peeling and recycling production line of spent li-ion batteries(liBs).Moreover,we specifically studied the leaching of fluorine(F)synergistically happened along with the removal process of Al,which was not concerned about in other studies,but one of the key factors affecting pollution prevention in the recovery *** mechanism of the whole process including the leaching of Al and F from the cathode powder was indicated by using NMR,FTIR,and XPS,and a defluoridation process was preliminarily investigated in this *** leaching kinetics of Al could be successfully described by the shrinking core model,controlled by the diffusion process and the activation energy was 11.14 kJ/***,the leaching of F was attributed to the dissolution of liPF6and decomposition of PVDF,and the kinetics associated was described by Avrami *** interaction of Al and F is advantageous to realize the defluoridation to some *** is expected that our investigation will provide theoretical support for the large-scale recycling of spent liBs.

关键词： Al removal F leaching Cathode powder Heating alkali leaching Leaching kinetics

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：