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Fishery analysis using gradient-dependent optimal interpolation

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Acta Oceanologica Sinica 2022年第2期41卷 116-126页

作者： chunling zhang Danyang Wang Zhenfeng Wang College of Marine Sciences Shanghai Ocean UniversityShanghai 201306China Project Management Office of China National Scientific Seafloor Observatory Tongji UniversityShanghai 200092China

The current lack of high-precision information on subsurface seawater is a constraint in fishery *** on Argo temperature and salinity profiles,this study applied the gradient-dependent optimal interpolation to reconstruct daily subsurface oceanic environmental information according to fishery dates and *** relationship between subsurface information and matching yellowfin tuna(YFT)in the western and central Pacific Ocean(WCPO)was examined using catch data from January 1,2008 to August 31,*** seawater temperature and salinity results showed differences of less than±0.5°C and±0.01 compared with the truth observations *** analysis revealed that the most suitable temperature for YFT fishery was 28–29°C at the *** most suitable salinity range for YFT fishery was 34.5–36.0 at depths shallower than 300 *** suitable upper and lower bounds on the depths of the thermocline were 90–100 m and 300–350 m,*** thermocline characteristics were prominent,with a mean temperature gradient exceeding 0.08°C/*** results indicate that the profiles constructed by gradient-dependent optimal interpolation were more accurate than those of the nearest profiles adopted.

关键词： gradient-dependent optimal interpolation YFT Argo profiles WCPO

On the microstructural evolution pattern toward nano-scale of an AISI 304 stainless steel during high strain rate surface deformation

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Journal of Materials Science & Technology 2020年第9期44卷 148-159页

作者： Hongwang zhang Yiming Zhao Yuhui Wang chunling zhang Yan Peng National Engineering Research Center for Equipment and Technology of Cold Strip Rolling College of Mechanical EngineeringYanshan UniversityQinhuangdao 066004China National Key Laboratory of Metastable Materials Science and Technology Yanshan UniversityQinhuangdao 066004China

In the present investigation,an austenitic AISI 304 stainless steel was subjected to high strain rate surface deformation by Pipe Inner-Surface Grinding(PISG)*** depth-dependent deformation parameters(strain,strain rate and strain gradient)were evaluated and the microstructures were systematically *** evolution from millimeter-to nano-scale was explored,with special attention paid to the localized *** evolution begins with the formation of planar dislocation arrays and the twin-matrix lamellae,which is followed by the localized deformation characterized by the initiation and the development of shear bands.A twinning-dominated process that was supplemented with dislocation slip-dominated one governed the microstructural evolution inside shear *** twin-matrix lamellae transform into extended/lamellar structure and finally the nanosized *** grains were substantially refined and martensitic transformation was effectively suppressed,of which the underlying mechanisms were analyzed.

关键词： Microstructural evolution Plastic deformation AISI 304 stainless steel Pipe inner-surface grinding Shear band

Fabrication of nanostructure in inner-surface of AISI 304 stainless steel pipe with surface plastic deformation

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Journal of Materials Science & Technology 2018年第11期34卷 2125-2130页

作者： Hongwang zhang Yiming Zhao Yuhui Wang Huaxin Yu chunling zhang National Engineering Research Center for Equipment and Technology of Cold Strip Rolling College of Mechanical EngineeringYanshan University National Key Laboratory of Metastable Materials Science and Technology Yanshan University

In the present investigation, a pipe inner-surface grinding（PISG） technique was developed to fabricate nanostructure in the inner-surface of an austenitic 304 stainless steel pipe. PISG was performed by high speed shearing with hard sphere tips, leading to gradient distribution of strain, strain rate and strain gradient along depth. Nano-austenite with an average boundary spacing of 20 nm was generated, followed by deformation microstructure characterized by shear bands, multi-and uni-directional twins and planar dislocation arrays. Deformation induced grain refinement of austenitic 304 stainless steel with low stacking fault energy（SFE） covering 4–5 order＇s magnitude of length scales toward nanometer regime was unified.

关键词： Nanostrucutre Pipe inner-surface grinding Stainless steel Plastic deformation Nano-austenite

Chrysin promotes osteogenic differentiation via ERK/MAPK activation

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Protein & Cell 2013年第7期4卷 539-547页

作者： Wenfeng Zeng Yan Yan Fayun zhang chunling zhang Wei Liang Protein&Peptide Pharmaceutical Laboratory Institute of BiophysicsChinese Academy of SciencesBeijing 100101China University of the Chinese Academy of Sciences Beijing 100049China Department of Endodontics Beijing Stomatological HospitalCapital Medical UniversityBeijing 100050China

T he effect of the anti-infl ammatory fl avonoid chrysin on osteogenesis was determined in preosteoblast MC3T3-E1 *** demonstrated that chrysin could induce osteogenic differentiation in the absence of other osteo-genic *** treatment promoted the expres-sion of transcription factors(Runx2 and Osx)and bone formation marker genes(Col1A1,OCN,and OPN)as well as enhanced the formation of mineralized *** osteogenic differentiation,chrysin preferentially activated ERK1/2,but not JNK nor the p38 *** experi-ments with inhibitors revealed the co-treatment of U0126,PD98059,or ICI182780(a general ER antagonist)with chrysin effectively abrogated the chrysin-induced osteo-genesis and ERK1/2 ***,the effect of chrysin on osteogenesis is ERK1/2-dependent and involves ***,chrysin has the signifi cant potential to enhance osteogenesis for osteoporosis prevention and treatment.

关键词： chrysin osteogenesis ERK1/2 estrogen re-ceptor

Effects of blade sliding cutting angle and stem level on cutting energy of rice stems

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International Journal of Agricultural and Biological Engineering 2019年第6期12卷 75-81页

作者： chunling zhang Liqing Chen Junfang Xia Jumin zhang College of Engineering Anhui Agricultural UniversityHefei 230036China Key Laboratory of Agricultural Equipment of the Mid-lower Reaches of Yangtze River Ministry of Agriculture and Rural AffairsHuazhong Agricultural UniversityWuhan 430070China

Previous studies highlighted the significance of optimizing the cutting blade for crop harvesting and size *** study investigated the effect of blade sliding cutting angle and stem level on cutting energy of single rice stem using a cutting apparatus that combined with texture *** cutting energy was determined for four blade *** results showed that the average cutting energy was the highest for cutting stem upper level and the lowest for cutting stem lower *** was found that the peak cutting force per unit stem area decreased with blade sliding cutting angle ***,the least average cutting energy was 9.12 J/mm^(2) of 45°sliding angle when cutting without counter support blade and 32.3%less than that of 60°sliding *** cutting with counter support blade,the cutting energy per unit stem area varied from 6.57 to 12.54 J/mm 2 as the sliding angle varied from 0°to 60°,whereas the peak cutting force per unit stem area varied from 2.46 to 0.98 N/mm *** was concluded that the optimal sliding cutting angle was 45°without support blade and 30°with support blade,*** experiments on rice stems in this study indicated that optimization of sliding cutting angle and stem level have a significant effect on cutting energy *** this study emphasized the need to further investigate the effect of the case of more moisture content and cutting speed on the cutting energy to help in selection of optimum cutting speed and harvesting time.

关键词： texture analyzer sliding cutting angle size reduction rice cutting energy

An effective method for improving the accuracy of Argo objective analysis

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Acta Oceanologica Sinica 2013年第7期32卷 66-77页

作者： zhang chunling XU Jianping BAO Xianwen WANG Zhenfeng Institute of Marine Environment Ocean University of China Second Institute of Oceanography State Oceanic Administration State Key Laboratory of Satellite Oceanography Environment Dynamics Second Institute of OceanographyState Oceanic Administration Marine Hydrologic Meteorological Center Command of Navy East China Sea Fleet

Based on the optimal interpolation objective analysis of the Argo data, improvements are made to the em- pirical formula of a background error covariance matrix widely used in data assimilation and objective anal- ysis systems. Specifically, an estimation of correlation scales that can improve effectively the accuracy of Ar- go objective analysis has been developed. This method can automatically adapt to the gradient change of a variable and is referred to as ＂gradient-dependent correlation scale method＂. Its effect on the Argo objective analysis is verified theoretically with Gaussian pulse and spectrum analysis. The results of one-dimensional simulation experiment show that the gradient-dependent correlation scales can improve the adaptability of the objective analysis system, making it possible for the analysis scheme to fully absorb the shortwave information of observation in areas with larger oceanographic gradients. The new scheme is applied to the Argo data obiective analysis system in the Pacific Ocean. The results are obviously improved.

关键词： gradient-dependent correlation scale background error covariance optimal interpolation,spectrum analysis Argo data

CRISPR/Cas9 technology and its application in horticultural crops

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Horticultural Plant Journal 2022年第4期8卷 395-407页

作者： Yang Liu chunling zhang Xiaofei Wang Xiuming Li Chunxiang You State Key Laboratory of Crop Biology College of Horticulture Science and EngineeringShandong Agricultural UniversityTai’anShandong 271018China

Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated 9(CRISPR/Cas9)system has recently become one popular technology due to its efficiency,precision,and simplicity compared with other genome editing tools such as Zinc Finger Nucleases(ZFNs)and Transcription Activator Like Effector Nucleases(TALENs).Horticultural crops provide energy and health-keeping nutrients to ***-editing technology has become widely adopted in horticultural breeding with the increasing demand for high yield and better-quality horticultural ***,we describe the CRISPR/Cas9 system construction,its optimization,including sgRNA promoter,sgRNA design,Cas9 protein promoter,SpCas9 variants and orthologs,and vector delivery *** also summarized the application of this technology in horticultural plants for stress responses enhancement,fruit quality improvement,and cultivation traits *** detailed review was compiled to help establish comprehensive understanding of the CRISPR/Cas9 systems and provide a reference for further developing this technology to manipulate horticultural plant traits effectively.

关键词： Gene editing CRISPR/Cas9 technology Horticultural plant

Design method of extractant for liquid-liquid extraction based on elements and chemical bonds

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Chinese Journal of Chemical Engineering 2024年第4期68卷 193-202页

作者： Yuwen Wei chunling zhang Yue zhang Lili Wang Li Xia Xiaoyan Sun Shuguang Xiang Institute of Process System Engineering College of Chemical EngineeringQingdao University of Science and TechnologyQingdao 266042China Yantai Guobang Chemical Machinery Technology Co. Ltd.Yantai 264002China

In the petrochemical industry process, the relative volatility between the components to be separated is close to one or the azeotrope that systems are difficult to separate. Liquid-liquid extraction is a common and effective separation method, and selecting an extraction agent is the key to extraction technology research. In this paper, a design method of extractants based on elements and chemical bonds was proposed. A knowledge-based molecular design method was adopted to pre-select elements and chemical bond groups. The molecules were automatically synthesized according to specific combination rules to avoid the problem of “combination explosion” of molecules. The target properties of the extractant were set, and the extractant meeting the requirements was selected by predicting the correlation physical properties of the generated molecules. Based on the separation performance of the extractant in liquid-liquid extraction and the relative importance of each index, the fuzzy comprehensive evaluation membership function was established, the analytic hierarchy process determined the mass ratio of each index, and the consistency test results were passed. The results of case study based on quantum chemical analysis demonstrated that effective determination of extractants for the analysis of benzene-cyclohexane systems. The results unanimously prove that the method has important theoretical significance and application value.

关键词： Molecular design Element and chemical bonds Molecular simulation Thermodynamics Solvent extraction

Naringenin reduces lung metastasis in a breast cancer resection model

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Protein & Cell 2011年第6期2卷 507-516页

作者： Lei Qin Lingtao Jin Linlin Lu Xiaoyan Lu chunling zhang Fayun zhang Wei Liang Protein&Peptide Pharmaceutical Laboratory National Laboratory of BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Graduate University of Chinese Academy of Sciences Beijing 100049China Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China

Metastasis is the main cause of death in cancer patients. To improve the outcomes of patients undergoing a surgery, new adjuvant therapies that can effectively inhibit metastases have to be developed. Studies have shown that flavonoid naringenin, a natural product that is mainly present in grapes and citrus, may contribute to cancer prevention. It has many advantages compared to traditional chemotherapeutic drugs, such as low toxicity. To determine whether naringenin can also inhibit metastases, a breast cancer resection model that mimics clinical situations was established. We found that orally administered naringenin significantly decreased the number of metastatic tumor cells in the lung and extended the life span of tumor resected mice. Flow cytometry analysis revealed that T cells displayed enhanced antitumor activity in naringenin treated mice, with an increased proportion of IFN-γ and IL-2 expressing T cells. In vitro studies further demonstrated that relief of immunosuppression caused by regulatory T cells might be the fundamental mechanism of metastasis inhibition by naringenin. These results indicate that orally administered naringenin can inhibit the outgrowth of metastases after surgery via regulating host immunity. Thus, naringenin can be an ideal surgical adjuvant therapy for breast cancer patients.

关键词： naringenin breast cancer surgery metastasis immunosuppression Tregs