检索结果-南通市图书馆

Improving word vector model with part-of-speech and dependency grammar information

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CAAI Transactions on Intelligence Technology 2020年第4期5卷 276-282页

作者： chunhui deng Gangming Lai Huifang deng School of Computer Engineering Guangzhou College of South China University of TechnologyNo.1 Xue Fu RoadHuaduGuangzhou 510800People’s Republic of China School of Computer Science and Engineering South China University of TechnologyUniversity TownPanyuGuanghzou 510006People’s Republic of China

Part-of-speech(POS)and dependency grammar(DG)are the basic components of natural language ***,current word vector models have not made full use of both POS information and DG information,and hence the models’performances are limited to some *** authors first put forward the concept of POS vector,and then,based on continuous bag-of-words(CBOW),constructed four models:CBOW+P,CBOW+PW,CBOW+G,and CBOW+G+P to incorporate POS information and DG information into word *** CBOW+P and CBOW+PW models are based on POS tagging,the CBOW+G model is based on DG parsing,and the CBOW+G+P model is based on POS tagging and DG *** information is integrated into the training process of word vectors through the POS vector to solve the problem of the POS similarity being difficult to *** POS vector correlation coefficient and distance weighting function are used to train the POS vector as well as the word *** information is used to correct the information loss caused by fixed context *** relations weight is used to measure the difference of dependency *** demonstrated the superior performance of their models while the time complexity is still kept the same as the base model of CBOW.

关键词： vector hence similarity

Advances in aptamer-based nanomaterials for separation and analysis of non-genetic biomarkers in biofluids

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2021年第6期64卷 932-947页

作者： Zidan Wang Chuwen Huang Nianrong Sun chunhui deng Department of Gastroenterology Zhongshan Hospital of Fudan UniversityShanghai 200032China Department of Chemistry Fudan UniversityShanghai 200032China

Body fluids contain a large number of disease markers including proteins,exosomes and cells,the concentration of which indicates the health status of a body system and can be hence applied to predict the degree of seriousness of a ***,the content of disease markers is relatively *** solving this problem,a variety of pretreatment methods have been developed,among which aptamer-based nanomaterials hold great potential for biomarker capture because of various natural properties of nano-substrates such as high specific surface area,as well as the high affinity of aptamer towards target and the higher stability than *** this review,we summarized the recent aptamer-based nanomaterials for the separation and analysis of different biomarkers including proteins,exosomes and *** specifically,the standard of classification description was based on the applied substrates including magnetic substrates and non-magnetic ***,the perspective of aptamer-based nanomaterials was discussed.

关键词： aptamers nanomaterials proteins cells exosomes biomarkers

Metal organic frameworks as advanced extraction adsorbents for separation and analysis in proteomics and environmental research

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2022年第4期65卷 650-677页

作者： Yonglei Wu Haolin Chen Yijie Chen Nianrong Sun chunhui deng Department of Gastroenterology and Hepatology Zhongshan HospitalFudan UniversityShanghai 200032China Department of Chemistry Institutes of Biomedical SciencesFudan UniversityShanghai 200433China

Human health is always under global spotlight, but now it suffers from severe environmental issue and various *** highly selective and effective extraction technique for environmental pollutant removal and target protein/peptide molecules conduces to the solution of environmental contamination and early disease detection, which will promise human health to a great degree. Metal-organic frameworks(MOFs) are typical porous nanomaterials featuring rigid structure, large specific surface area, thermal and chemical stability, numerous active sites and easy functionalization. Moreover, multitudinous combination of metal centers and organic ligands often endows MOFs with structure and function diversity. Owing to such splendid merits, MOFs are able to act as advanced adsorbents for sample-pretreatment in proteomics and environmental field,which indicates great efficiency, reproducibility, convenience and cost-effectiveness. In this review, the novel design of MOFs and their applications in proteomics and environmental research are roughly summarized. We first introduce the representative researches of functional MOFs in protein post-translational modifications including glycosylation and phosphorylation, mainly focusing on hydrophilic interaction liquid chromatography(HILIC) or metal-oxide affinity chromatography(MOAC). The important role of MOFs in enzyme immobilization is also covered. Then MOF-based adsorptive removal of diverse environmental contaminants embracing plasticizers, pesticides and varied water pollutants(pharmaceutical and personal care products,organic dyes and heavy metals), in which multiple chemical and non-chemical interactions play a vital role, is emphatically discussed. Finally, we analyze the existing problems and challenges in the way of wider practical applications and put forward prospective anticipation.

关键词： metal-organic frameworks adsorbent sample pre-treatment proteomics research environmental research

同方期刊数据库

Removal of Microcystin-LR in lake water sample by hydrophilic mesoporous silica composites under high-throughput MALDI-TOF MS detection platform

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第5期35卷 380-385页

作者： Wantong Zhang Zixing Xu Guofei Dai Zhijian Li chunhui deng Department of Chemistry Institute of Metabolism&Integrate Biology(IMIB)Fudan UniversityShanghai 200433China Jiangxi Key Laboratory of Organic Chemistry Jiangxi Science and Technology Normal UniversityNanchang 330013China Jiangxi Academy of Water Science and Engineering Nanchang 330029China

Microcystins(MCs),a family of cyclic heptapeptide cyanotoxins,exists in aquatic environment where cyanobacterial bloom happens,which will accumulate in aquatic organisms and transfer through the food chain to higher trophic levels,posing a health risk to both animals and human *** various MCs,Microcystin-LR(MC-LR)is worthiest studied for its strong toxicity,ubiquity and *** in this work,iminodiacetic acid(IDA)decorated magnetic mesoporous silica(mSiO_(2))nanocomposites(Fe_(3)O_(4)@mSiO_(2)-IDA)were facilely synthesized which possessed the merits of large surface area(188.21 m^(2)/g),accessible porosity(2.66 nm),excellent hydrophilicity and rapid responsiveness to magnetic *** the composites were successfully employed to the removal process of Microcystin-LR in real water samples followed by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS)analysis,achieving the removal efficiency above 92.5%even after ten recycles of the *** provided a potential method for removing MC-LR in aqueous environment with high effectiveness,lower costs and less secondary contamination.

关键词： Microcystin-LR Mesoporous silica Magnetic composites MALDI-TOF MS analysis

Sulfonic acid-based metal organic framework functionalized magnetic nanocomposite combined with gas chromatography-electron capture detector for extraction and determination of organochlorine

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2020年第7期31卷 1843-1846页

作者： Ying Wang Qing Ye Menghuan Yu Xijun Zhang chunhui deng School of chemistry and Environmental Science Shangrao Normal UniversityShangrao 334001China Department of Chemistry Fudan UniversityShanghai 200433China

The metal organic framework functionalized with sulfonic acid was combined with magnetic nanoparticles to fabricate a new nanocomposite(denoted as Fe3O4@PDA@Zr-SO3H).By combining with gas chromatography-electron capture detector,the resulting Fe3O4@PDA@Zr-SO3H nanocomposite was successfully used as a high-efficiency adsorbent for pre-concentrating eight organochlorine pesticides from water sample in *** from the ability of fast separation,the as-prepared Fe3O4@PDA@Zr-SO3H nanocomposite also exhibited high adsorption capacity for organochlorine *** the use of optimal experimental conditions,the linear relationship can be obtained in the range of 0.05~300μg/L,the correlation coefficient was over 0.9978,and the relative standard deviation was located in 2.5%-7.7%.Moreover,the limit of detection and quantification was between0.005-0.016μg/L and 0.017~0.050μg/***,the nanocomposite was used for the determination of organochlorine pesticides from environmental water samples,and displayed the recovery of 82%-118%.

关键词： Organochlorine pesticides Metal-organic framework Magnetic nanocomposite Water Gas chromatography-electron capture detector

Probing region-resolved heterogeneity of phosphoproteome in human lens by hybrid metal organic frameworks

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2023年第1期34卷 583-587页

作者： Huimin Chu Haoyang Zheng Aizhu Miao chunhui deng Nianrong Sun Department of Gastroenterology and Hepatology Zhongshan Hospitaland Department of ChemistryFudan UniversityShanghai 200032China Institue of Metabolism&Integrate Biology(IMIB) Fudan UniversityShanghai 200433China Department of Ophthalmology Eye&ENT Hospital of Fudan UniversityShanghai 200031China

Phosphorylation plays crucial parts in lenticular biological *** knowledge of region-resolved phosphoproteome contributes to better comprehending the ***,we prepared the hybrid metal organic frameworks(HMOFs)for probing the region-resolved heterogeneity of phosphoproteome in human lens.1334 phosphosites corresponding to 564 phosphoproteins,1160 phosphosites corresponding to 316 phosphoproteins and 517 phosphosites corresponding to 205 phosphoproteins were identified in capsule,cortex and nucleus,respectively,providing the relatively extensive distribution mapping of phosphorylation in human lens for the first *** label-free quantification experiments and principal component analysis presented differential expression of phopshoproteins in three *** instance,α-crystallin,β-crystallin and fibrillin-1 closely associated with cataract and Marfan syndrome showed disparate spatial *** preferential phosphoproteins in capsule,cortex and nucleus were involved in cytoskeleton organization,metabolic process and lens development in camera-type eye,*** work first provided a general overview of region-resolved phosphoproteome of human lens.

关键词： Phosphoproteome Human lens Region resolution Metal organic framework Metal oxide affinity chromatography Immobilized metal affinity chromatography

Inherently hydrophilic mesoporous channel coupled with metal oxide for fishing endogenous salivary glycopeptides and phosphopeptides

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第10期33卷 4695-4699页

作者： Zixing Xu Yonglei Wu Xufang Hu chunhui deng Nianrong Sun Department of Chemistry Fudan UniversityShanghai 200433China Department of Gastroenterology and Hepatology Zhongshan HospitalFudan UniversityShanghai 200032China School of Chemical Science and Technology Yunnan UniversityKunming 650091China

Both glycosylation and phosphorylation exert crucial rule in multitudinous biological *** in-depth profiling of glycosylation and phosphorylation,a magnetic metal oxide is effectively coupled with inherently hydrophilic mesoporous channels(denoted as Fe_(3)O_(4)@TiO_(2)@mSiO_(2)-TSG).Based on the mechanism of hydrophilic interaction liquid chromatography(HILIC)and metal oxide affinity chromatography(MOAC),the Fe_(3)O_(4)@TiO_(2)@mSiO_(2)-TSG nanomaterial shows high capacity for simultaneously enriching glycopeptides and *** human saliva collected in successive four days as practical biological sample,endogenous glycopeptides and phosphopeptides are efficiently *** gene ontology analysis reveals that the identified endogenous glycopeptides and phosphopeptides participate in diverse molecular functions and biological *** strategy is anticipated to promote variation analysis of salivary post-translational modifications.

关键词： Mesoporous material Hydrophilic interaction liquid chromatography Metal oxide affinity chromatography Human saliva Endogenous phosphopeptides Endogenous glycopeptides

Peptidome data-driven comprehensive individualized monitoring of membranous nephropathy with machine learning

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第5期35卷 402-406页

作者： Zixing Xu Ruiying Chen Chuanming Hao Qionghong Xie chunhui deng Nianrong Sun Department of Gastroenterology and Hepatology Zhongshan Hospitaland Department of ChemistryFudan UniversityShanghai 200433China Division of Nephrology Huashan HospitalFudan UniversityShanghai 200040China School of Chemistry and Chemical Engineering Nanchang UniversityNanchang 330031China

As the most common pathological type of nephrotic syndrome,membranous nephropathy(MN)presents diversity in progression trends,facing severe *** precise discrimination of MN from healthy people,other types of nephrotic syndrome or those with therapeutic remission has always been huge challenge in clinics,not to mention comprehensive individualized monitoring relied on minimally invasive molecular detection ***,we construct a functionalized pore architecture to couple with machine learning to aid all-round peptidome enrichment and data profiling from hundreds of human serum samples,and finally establish a set of defined peptide panel consisting of 12 specific feature *** addition to the realization of above-mentioned precise discrimination with more than 97%of sensitivity,88%of accuracy and f1 score,the simultaneously comprehensive individualized monitoring for MN can also be achieved,including conventionally screening diagnosis,congeneric distinction and prognostic *** work greatly advances the development of peptidome data-driven individualized monitoring means for complex diseases and undoubtedly inspire more devotion into molecular detection field.

关键词： Membranous nephropathy Serum peptidome Machine learning Disease diagnosis

Specific enrichment of urinary exosomes and exosomal glycopeptides by coefficient affinity of integrated L-cysteine and titania

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2023年第2期34卷 254-258页

作者： Yijie Chen Haolin Chen Chenjie Yang Yonglei Wu chunhui deng Nianrong Sun Department of Chemistry Institute of Metabolism&Integrate Biology(IMIB)Fudan UniversityShanghai 200433China Department of Gastroenterology and Hepatology Zhongshan HospitalFudan UniversityShanghai 200032China

Exosome and inclusive cargoes have manifested significant function in different biological events. In particular, glycopeptides in exosome are closely associated with occurrence and development of various diseases. Developing advanced tools is highly desired to enrich glycopeptides from exosomes, and enrich exosomes from complex biological samples as well. In this work, integration of L-cysteine and titania onto the surface of magnetic nanoparticles is designed to realize the coefficient affinity towards exosomes and inclusive glycopeptides. Benefiting from the synergistic affinity, we separate exosomes from human urine concentrate directly, which was proved by the detection of three typical antigen markers of exosomes. Furthermore, hardly any exosomes remained on materials after ultrasonication, which confirmed the good capture performance of Fe_(3)O_(4)@TiO_(2)@L-Cys and high release effect of direct ***, 146 glycopeptides corresponding to 77 glycoproteins were successfully identified from captured exosomes. These satisfactory results will inspire more efforts to be devoted to this field and will be extremely helpful to in-depth information excavation of biological markers, especially disease-related ones, through exosomes and inclusive glycopeptides.

关键词： Glycopeptides Enrichment Nanomaterials Exosomes Urine