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Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

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World Journal of Gastroenterology 2008年第8期14卷 1204-1211页

作者： Xin-Ying Wang Zhou-Sheng Lai chung-man yeung Ji-De Wang Wen Deng Hoi Yee Li Yu-Jing Han Hsiang-Fu Kung Bo Jiang Marie Chia-mi Lin Department of Gastroenterology Nanfang HospitalNanfang Medical University Tong He Guangzhou 510520Guangdong Province China Departmentof Chemistry the University of Hong Kong Pokfulam RoadHong Kong China Department of Anatomy the University of HongKong Pokfulam Road Hong Kong China Li Ka Shing Institute of Health Sciences The Centre for Emerging Infectious Diseases the Chinese Universityof Hong Kong Hong Kong China

AIM:To study the molecular mechanism of laterally spreading tumor (LST), a cell line [Laterally Spreading Tumor-Rectum 1 (LST-R1)] was derived and the characteristics of this cell line were investigated. METHODS:A new cell line (LST-R1) originated from laterally spreading tumor was established. Properties of the cell line were characterized using scanning and transmission electron microscopy, immunohistochemistry method, cytogenetic analysis and nude mice xenograft experiments. In vitro invasion assay, cDNA microarray and Western blotting were used to compare the difference between the LST-R1 and other colorectal cancer cell lines derived from prudent colon cancer. RESULTS:Our study demonstrated that both epithelial special antigen (ESA) and cytokeratin-20 (CK20) were expressed in LST-R1. The cells presented microvilli and tight junction with large nuclei. The karyotypic analysis showed hyperdiploid features with structural chromosome aberrations. The in vivo tumorigenicity was also demonstrated in nude mice xenograft experiments. The invasion assay suggested this cell line has a higher invasive ability. cDNA microarray and Western blotting show the loss of the expression of E-cadherin in LST-R1 ***:We established and characterized a colorectal cancer cell line, LST-R1 and LST-R1 has an obvious malignant tendency, which maybe partially attributed to the changes of the expression of some adhesion molecules, such as E-cadherin. It is also a versatile tool for exploring the original and progressive mechanisms of laterally spreading tumor and the early colon cancer genesis.

关键词： LST-R1 Cell line Invasion E-cadherin

Pluronic L-81 ameliorates diabetic symptoms in db/db mice through transcriptional regulation of microsomal triglyceride transfer protein

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World Journal of Gastroenterology 2009年第24期15卷 2987-2994页

作者： Wo-Shing Au Li-Wei Lu Sidney Tam Otis King Hung Ko Billy KC Chow Ming-Liang He Samuel S Ng chung-man yeung Ching-Chiu Liu Hsiang-Fu Kung Marie C Lin Institute of Molecular Technology for Drug Discovery and Synthesis Department of Chemistry The University of Hong Kong Hong Kong China Department of Pathology The University of Hong Kong Hong Kong China Division of Clinical Biochemistry Queen Mary Hospital Hong Kong China School of Biological Sciences The University of Hong Kong China Stanley Ho Center for Emerging Infectious Diseases The Chinese University of Hong Kong Shatin Hong Kong China

AIM: To test whether oral L-81 treatment could im-prove the condition of mice with diabetes and to investigate how L-81 regulates microsomal triglyceride transfer protein (MTP) activity in the liver. METHODS: Genetically diabetic (db/db) mice were fed on chow supplemented with or without L-81 for 4 wk. The body weight, plasma glucose level, plasma lipid profile, and adipocyte volume of the db/db mice were assessed after treatment. Toxicity of L-81 was also evaluated. To understand the molecular mecha-nism, HepG2 cells were treated with L-81 and the effects on apolipoprotein B (apoB) secretion and mRNA level of the MTP gene were ***: Treatment of db/db mice with L-81 sig-nificantly reduced and nearly normalized their body weight, hyperphagia and polydipsia. L-81 also markedly decreased the fasting plasma glucose level, improved glucose tolerance, and attenuated the elevated levels of plasma cholesterol and triglyceride. At the effective dosage, little toxicity was observed. Treatment of HepG2 cells with L-81 not only inhibited apoB secretion, but also signif icantly decreased the mRNA level of the MTP gene. Similar to the action of insulin, L-81 exerted its effect on the MTP promoter. CONCLUSION: L-81 represents a promising candidate in the development of a selective insulin-mimetic mol-ecule and an anti-diabetic agent.

关键词： Pluronic L-81 db/db mice Animal models diabetes Microsomal triglyceride protein

Interplay of laser power and pore characteristics in selective laser melting of ZK60 magnesium alloys:A study based on in-situ monitoring and image analysis

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Journal of Magnesium and Alloys 2024年第4期12卷 1346-1366页

作者： Weijie Xie Hau-chung man Chi-Wai Chan Department of Industrial and Systems Engineering The Hong Kong Polytechnic UniversityHong KongChina School of Mechanical and Aerospace Engineering Queen’s UniversityBelfast BT95AHUK Research Institute for Advanced manufacturing The Hong Kong Polytechnic UniversityHong KongChina

This study offers significant insights into the multi-physics phenomena of the SLM process and the subsequent porosity characteristics of ZK60 Magnesium(Mg)***-speed in-situ monitoring was employed to visualise process signals in real-time,elucidating the dynamics of melt pools and vapour plumes under varying laser power conditions specifically between 40 W and 60 *** morphological analysis was performed using Scanning-Electron Microscopy(SEM),demonstrating a critical correlation between laser power and pore *** laser power led to increased pore coverage,whereas a denser structure was observed at higher laser *** laser power influence on porosity was further confirmed via Optical Microscopy(OM)conducted on both top and cross-sectional surfaces of the *** increase in laser power resulted in a decrease in pore coverage and pore size,potentially leading to a denser printed part of Mg alloy.X-ray Computed Tomography(XCT)augmented these findings by providing a 3D volumetric representation of the sample internal structure,revealing an inverse relationship between laser power and overall pore *** laser power appeared to favour the formation of interconnected pores,while a reduction in interconnected pores and an increase in isolated pores were observed at higher *** interplay between melt pool size,vapour plume effects,and laser power was found to significantly influence the resulting porosity,indicating a need for effective management of these factors to optimise the SLM process of Mg alloys.

关键词： Selective laser melting(SLM) Magnesium(Mg)alloys Biodegradable implants Porosity In-situ monitoring

Neuronal damage and blood-retinal barrier disruption in retinal ischemia/reperfusion injury:protective effects of Lycium barbarum polysaccharides

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Neuronal damage and blood-retinal barrier disruption in reti...

Neuroprotective effects of Lycium barbarum polysaccharides on acute ischemic stroke injury

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Amy Cheuk Yin Lo Suk-Yee Li chung-man yeung Raymond Chuen-chung Chang Kwok-Fai So David Wong Eye Institute Li Ka Shing Faculty of MedicineThe University of Hong Kong Research Center of Heart BrainHormone and Healthy AgingLi Ka Shing Faculty of MedicineThe University of Hong Kong Department of Anatomy Li Ka Shing Faculty of MedicineThe University of Hong Kong

＜正＞Objective Neuronal cell death,glial cell activation,retinal swelling and oxidative injury are complications in retinal ischemia/reperfusion（I/R） *** barbarum polysaccharides（LBP）,extracts from the wolfberries, are good for "eye health" in Chinese *** aim of our present study is to explore the use of LBP in retinal I/R *** Retinal I/R injury was induced by surgical occlusion of the internal carotid *** to induction of ischemia,mice were fed orally with either vehicle（PBS） or LBP（1 mg/kg） once a day for 1 week. Ischemia was maintained for 2 hours and reperfusion was then allowed for 22 ***-embedded retinal sections were *** cells were counted while apoptosis was assessed using TUNEL *** levels of glial fibrillary acidic protein（GFAP）,aquaporin-4（AQP4）,poly（ADP-ribose）（PAR） and nitrotyrosine （NT） were investigated by *** integrity of blood-retinal barrier（BRB） was examined by IgG extravasation *** Decreased viable cell count and apoptosis were found in the ganglion cell layer （GCL） and the inner nuclear layer（INL） of the vehicle-treated I/R ***,increased retinal thickness, GFAP activation,AQP4 up-regulation,IgG extravasation and PAR expression level were observed in the vehicletreated I/R *** importantly,many of these changes were diminished or abolished in the LBP-treated I/R *** Pre-treatment with LBP for 1 week effectively protected the retina from neuronal death,apoptosis, glial cell activation,aquaporin water channel up-regulation,disruption of BRB and oxidative *** present study suggests that LBP may have a neuroprotective role in ocular diseases in which I/R is a feature.

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Neuroprotective effects of Lycium barbarum polysaccharides o...

Immunoregulatory and Anti-cancer Activities of Combination Treatment of Novel Four-Herb Formula and Doxorubicin in 4T1-Breast Cancer Bearing Mice

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Amy Cheuk-Yin LO chung-man yeung Suk-Yee LI Raymond Chuen-chung CHANG Kwok-Fai SO David WONG Eye Institute Li Ka Shing Faculty of MedicineThe University of Hong Kong Research Center of Heart BrainHormone and Healthy AgingLi Ka Shing Faculty of MedicineThe University of Hong Kong Department of Anatomy Li Ka Shing Faculty of MedicineThe University of Hong Kong St Paul’s Eye Unit University of LiverpoolUK

＜正＞Objective The polysaccharides in Lycium barbarum,a well known traditional Chinese medicine,have been demonstrated to display multiple biological effects such as anti-aging,anti-tumor and *** the present study,we aimed to evaluate the effects of Lycium barbarum polysaccharides（LBP） on murine brain injury after induction of acute ischemic stroke by middle cerebral artery occlusion（MCAO）.Methods Mice were orally treated with either vehicle（PBS） or LBP（1 mg/kg or 10 mg/kg） for 1 week before induction of cerebral ischemia by *** 2 h of ischemia followed by 22 h of reperfusion,animals were evaluated for neurological *** after scoring of the neurological deficits,brains were isolated,cut into 6 coronal slices of 2 mm thickness and stained with 2%2,3,5-triphenyltetrazolium chloride（TTC） to detect lesion *** posterior surface of each brain slice was subsequently photographed and analyzed using a digital image analysis *** area,volume,and hemispheric brain swelling were calculated using an indirect method in which the effects of edema and brain swelling have been ***（1） Mice treated with either 1 mg/kg LBP or 10 mg/kg LBP（n=7 for each group） had less neurological deficits than the vehicle-treated mice（n=8）（vehicle=2.0+/-0.3,1 mg/kg LBP=1.4+/-0.2,10 mg/kg LBP=1.1+/-0.1）.（2） TTC staining revealed a significantly smaller infarct area in brain slice number 4 in the LBP-treated mice when compared with the vehicle-treated mice（vehicle=35.4+/- 4.2%,1 mg/kg LBP=17.5+/-5.8%,10 mg/kg LBP=17.6+/-4.9%）.（3） Consistent with the infarct area data,the overall infarct volumes were decreased in the LBP-treated groups（vehicle=25.8+/-3.1%,1 mg/kg LBP=215+/-2.5%,10mg/kg LBP=16.6+/-2.7%）. （4） Mice treated with LBP displayed less hemispheric brain swelling（vehicle=11.1+/-0.9%,1 mg/kg LBP=9.7+/-1.1%,10mg/kg LBP=7.9+/-0.6%）.Conclusions Taken together,these data indicate that treatment with LBP for 1 week could protect the mouse

关键词： neuroprotection animal model oxidative stress

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Chinese Journal of Integrative Medicine 2024年第4期30卷 311-321页

作者： Lea Ling-Yu Kan Ben chung-Lap Chan Grace Gar-Lee Yue LI Pei-ting Sharon Sze-man Hon HUANG Dan-qi Miranda Sin-man Tsang Clara Bik-San Lau Ping-chung Leung Chun-Kwok Wong Institute of Chinese Medicine The Chinese University of Hong KongHong Kong SARChina State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants The Chinese University of Hong KongHong KongChina Department of Chemical Pathology The Chinese University of Hong KongHong Kong SARChina China-Australia International Research Centre for Chinese Medicine School of Health and Biomedical SciencesSTEM CollegeRMIT UniversityBundooraVictoriaAustralia Li Dak Sum Yip Yio Chin R&D Centre for Chinese Medicine The Chinese University of Hong Kong SARHong KongChina

Objective To investigate the in vivo immunomodulatory and anti-tumor mechanisms of the combined treatment of novel Four-Herb formula(4HF)and doxorubicin in triple-negative breast cancer(TNBC).Methods Murine-derived triple-negative mammary carcinoma cell line,4T1 cells,was cultured and inoculated into mouse mammary ***-six mice were randomly assigned into 6 groups(n=11 in ench):naive,control,LD 4HF(low dose 4HF),HD 4HF(high dose 4HF),LD 4HF+D(low dose and doxorubicin),and D(doxorubicin).Apart from the naive group,each mouse received subcutaneous inoculation with 5×10^(5)4T1 cells resuspended in 100µL of normal saline in the mammary fat *** from the day of tumor cell inoculation,tumors were grown for 6 *** LD and HD groups received daily oral gavage of 658 and 2,630 mg/kg 4HF,*** LD 4HF+D group received daily oral gavage of 658 mg/kg 4HF and weekly intraperitoneal injection of doxorubicin(5 mg/kg).The D group received weekly intraperitoneal injections of doxorubicin(5 mg/kg).The treatment naive mice received daily oral gavage of 0.2 mL double distilled water and 0.1 mL normal saline via intraperitoneal injection once a *** control group received daily oral gavage of 0.2 mL double-distilled *** treatment period was 30 *** the end of treatment,mice organs were harvested to analyze immunological activities via immunophenotyping,gene and multiplex analysis,histological staining,and gut microbiota *** Mice treated with the combination of 4HF and doxorubicin resulted in significantly reduced tumor and spleen burdens(Pmanipulated gut microbiota to favor the anti-tumor immunological ***,immunosuppressive genes,cytokines,and chemokines such as C-C motif chemokine 2 and interleukin-10 of tumors were significantly downregulated(P<0.05).4HF-doxorubicin combination treatment demonstrated synergetic activities and was most effective

关键词： adjunctivetherapy breast cancer cytokines Chinese medicine tumor microenvironment

Indications of pro-inflammatory cytokines in laparoscopic and open liver resection for early-stage hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2024年第3期23卷 257-264页

作者： Kevin Tak-Pan Ng Li Pang Jia-Qi Wang Wong Hoi She Simon Hing-Yin Tsang chung Mau Lo Kwan man Tan To Cheung Department of Surgery HKU-SZH&School of Clinical MedicineLi Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina State Key Laboratory of Liver Research The University of Hong KongHong KongChina

Background:Our clinical practice of laparoscopic liver resection(LLR)had achieved better short-term and long-term benefits for patients with hepatocellular carcinoma(HCC)over open liver resection(OLR),but the underlying mechanisms are not *** study was to find out whether systemic inflammation plays an important ***:A total of 103 patients with early-stage HCC under liver resection were enrolled(LLR group,n=53;OLR group,n=50).The expression of 9 inflammatory cytokines in patients at preoperation,postoperative day 1(POD1)and POD7 was quantified by Luminex Multiplex *** relationships of the cytokines and the postoperative outcomes were compared between LLR and ***:Seven of the circulating cytokines were found to be significantly upregulated on POD1 after LLR or OLR compared to their preoperative *** to OLR,the POD1 levels of granulocytemacrophage colony-stimulating factor(GM-CSF),interleukin-6(IL-6),IL-8,and monocyte chemoattractant protein-1(MCP-1)in the LLR group were significantly *** POD1 levels of these cytokines were significantly correlated with longer operative time and higher volume of blood loss during *** levels of these cytokines were positively associated with postoperative liver injury,and the length of hospital ***,a high level of IL-6 at POD1 was a risk factor for HCC recurrence and poor disease-free survival after liver ***:Significantly lower level of GM-CSF,IL-6,IL-8,and MCP-1 after liver resection represented a milder systemic inflammation which might be an important mechanism to offer better short-term and long-term outcomes in LLR over OLR.

关键词： Laparoscopic liver resection Open liver resection Hepatocellular carcinoma Postoperative cytokines Inflammatory responses

Unique anti-angiogenic effects,pharmacological targets and therapeutic mechanisms of Chinese herbal medicines for endometriosis

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Genes & Diseases 2024年第5期11卷 63-65页

作者： Bo Liang Rui Dong Sze Wan Hung Yiran Li Yuezhen Lin Ling Wu Tao Zhang Gene Chi Wai man Hui Xu Jacqueline Pui Wah chung Chi Chiu Wang Guangdong Provincial Key Laboratory of Marine Biotechnology Institute of Marine SciencesShantou UniversityShantouGuangdong 515063China Department of Obstetrics and Gynaecology The Chinese University of Hong KongHong Kong SARChina Reproduction and Development Laboratory Li Ka Shing Institute of Health SciencesThe Chinese University of Hong KongHong Kong SARChina School of Biomedical Sciences The Chinese University of Hong KongHong Kong SARChina Chinese University of Hong Kong-Sichuan University Joint Laboratory in Reproductive Medicine The Chinese University of Hong KongHong Kong SARChina

Endometriosis is a common and benign angiogenesisdependent gynecological disorder,which refers to the proliferation and growth of endometrium-like tissues with neovasculature formation outside of the uterus.1 Available medical treatments for endometriosis containing hormonal and non-hormonal treatments had been limited for longterm usage by their side effects.2 Ideal medical treatment for endometriosis with efficacy to relieve symptoms and suppress endometriotic lesion growth and minimal side effects has been longing for decades.3 Angiogenesis is a promising therapeutic target for endometriosis.

关键词： therapeutic endometriosis herbal

Distinct molecular targets of ProEGCG from EGCG and superior inhibition of angiogenesis signaling pathways for treatment of endometriosis

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Journal of Pharmaceutical Analysis 2024年第1期14卷 100-114页

作者： Sze Wan Hung Massimiliano Gaetani Yiran Li Zhouyurong Tan Xu Zheng Ruizhe Zhang Yang Ding Gene Chi Wai man Tao Zhang Yi Song Yao Wang Jacqueline Pui Wah chung Tak Hang Chan Roman A.Zubarev Chi Chiu Wang Department of Obstetrics&Gynaecology The Chinese University of Hong KongHong KongChina Division of Physiological Chemistry I Department of Medical Biochemistry and BiophysicsKarolinska InstitutetStockholmSE 17177Sweden Chemical Proteomics Core Facility Department of Medical Biochemistry and BiophysicsKarolinska InstitutetStockholmSE 17177Sweden Unit of Chemical Proteomics Science for Life Laboratory(SciLifeLab)StockholmSE 17177Sweden Center for Reproductive Medicine Henan Key Laboratory of Reproduction and GeneticsThe First Affiliated Hospital of Zhengzhou UniversityZhengzhou450003China Department of Chemistry McGill UniversityMontrealH3A2K6Canada Department of Pharmacological&Technological Chemistry I.M.Sechenov First Moscow State Medical UniversityMoscow119146Russia Reproduction and Development Li Ka Shing Institute of Health SciencesThe Chinese University of Hong KongHong KongChina School of Biomedical Sciences The Chinese University of Hong KongHong KongChina Chinese University of Hong Kong-Sichuan University Joint Laboratory in Reproductive Medicine The Chinese University of Hong KongHong KongChina

Endometriosis is a common chronic gynecological disease with endometrial cell implantation outside the *** is a major pathophysiology in *** previous studies have demonstrated that the prodrug of epigallocatechin gallate(ProEGCG)exhibits superior anti-endometriotic and anti-angiogenic effects compared to epigallocatechin gallate(EGCG).However,their direct binding targets and underlying mechanisms for the differential effects remain *** this study,we demonstrated that oral ProEGCG can be effective in preventing and treating ***,1D and 2D Proteome Integral Solubility Alteration assay-based chemical proteomics identified metadherin(MTDH)and PX domain containing serine/threonine kinase-like(PXK)as novel binding targets of EGCG and ProEGCG,*** simulation and BioLayer interferometry were used to confirm their binding *** results showed that MTDH-EGCG inhibited protein kinase B(Akt)-mediated angiogenesis,while PXK-ProEGCG inhibited epidermal growth factor(EGF)-mediated angiogenesis via the EGF/hypoxia-inducible factor(HIF-1a)/vascular endothelial growth factor(VEGF)*** vitro and in vivo knockdown assays and microvascular network imaging further confirmed the involvement of these signaling ***,our study demonstrated that ProEGCG has superior therapeutic effects than EGCG by targeting distinct signal transduction pathways and may act as a novel antiangiogenic therapy for endometriosis.

关键词： Molecular targets ProEGCG EGCG Angiogenesis Treatment Endometriosis