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Functional analysis of the acetylation of human p53 in DNA damage responses

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Protein & Cell 2014年第7期5卷 544-551页

作者： Sun-Ku chung Shengyun Zhu Yang Xu Xuemei Fu Shenzhen Children's Hospital 7019 Yitian Road Shenzhen Guangdong 518026 China Division of Biological Sciences University of California San Diego 9500 Gilman Drive La Jolla CA 92093 USA Chongqing Medical University Chongqing 400016 China

As a critical tumor suppressor, p53 is inactivated in human cancer cells by somatic gene mutation or disruption of pathways required for its activation. Therefore, it is critical to elucidate the mechanism underlying p53 activation after genotoxic and cellular stresses. Accumulating evidence has indicated the importance of posttranslational modifications such as acetylation in regulating p53 stability and activity. However, the physiological roles of the eight identified acetylation events in regulating p53 responses remain to be fully understood. By employing homologous recombination, we introduced various combinations of missense mutations （lysine to arginine） into eight acetylation sites of the endogenous p53 gene in human embryonic stem cells （hESCs）. By determining the p53 responses to DNA damage in the p53 knock-in mutant hESCs and their derivatives, we demonstrate physiological importance of the acetylation events within the core domain （kt20 and K164） and at the C-terminus （K370/372/373/381/382/ 386） in regulating human p53 responses to DNA damage.

关键词： human embryonic stem cells (hESCs) p53 acetylation homologous recombination DNA damage cancer

Successful kidney transplantation from an expanded criteria donor with long-term extracorporeal membrane oxygenation treatment: A case report

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World Journal of Clinical Cases 2020年第3期8卷 540-545页

作者： Hye Won Seo Sua Lee Hwa Young Lee Sun Cheol Park Byung Ha chung Chul Woo Yang Tae Hyun Ban Division of Nephrology Department of Internal MedicineSeoul St.Mary’s HospitalCollege of MedicineThe Catholic University of KoreaSeoul 06591South Korea Transplant Research Center Department of Internal MedicineSeoul St.Mary’s HospitalCollege of MedicineThe Catholic University of KoreaSeoul 06591South Korea Division of Nephrology Department of Internal MedicineJeju National University HospitalJeju-do 63241South Korea Department of Surgery Seoul St.Mary’s HospitalCollege of MedicineThe Catholic University of Korea222 Banpo-daeroSeoul 06591South Korea Division of Nephrology Department of Internal MedicineEunpyeong St.Mary’s HospitalCollege of MedicineThe Catholic University of KoreaEunpyeong-guSeoul 03312South Korea

BACKGROUND Due to a shortage of donor kidneys, many centers have utilized graft kidneys from brain-dead donors with expanded criteria. Kidney transplantation(kt)from donors on extracorporeal membrane oxygenation(ECMO) has been identified as a successful way of expanding donor pools. However, there are currently no guidelines or recommendations that guarantee successful kt from donors undergoing ECMO treatment. Therefore, acceptance of appropriate allografts from those donors is solely based on clinician *** SUMMARY We report a case of successful kt from a brain-dead donor supported by ECMO for the longest duration to date. A 69-year-old male received a kt from a 63-yearold brain-dead donor who had been on therapeutic ECMO treatment for the previous three weeks. The recipient experienced slow recovery of graft function after surgery but was discharged home on post-operative day 17 free from hemodialysis. Allograft function gradually improved thereafter and was comparatively acceptable up to the 12 mo follow-up, with serum creatinine level of 1.67 mg/d *** This case suggests that donation even after long-term ECMO treatment could provide successful kt to suitable candidates.

关键词： Extracorporeal membrane oxygenation Kidney transplantation Delayed graft function Donor selection Case report

Ascorbic Acid Attenuates Acute Ethanol-Induced Liver Injury in SMP30 Knockout Mice

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Food and Nutrition Sciences 2017年第12期8卷 1114-1126页

作者： Eun-Mi Cho H. M. Arif Ullah Ahmed K. Elfadl Myung-Jin chung Soong-Koo Kim Yong Deuk Kim Eun-Joo Lee Kyu-Shik Jeong College of Veterinary Medicine Kyungpook National University Daegu Republic of Korea

Ascorbic acid (AA) is recognized as a free radical scavenger that protects cells from oxidative stress-induced damage. However, no studies have investigated the role of AA in acute alcoholic liver disease using senescence marker protein-30 (SMP30) knockout (KO) mice. SMP30 is a novel 34-kDa protein involved in AA biosynthesis. The present study aimed to elucidate the physiological functions of AA in acute ethanol-induced liver injury using SMP30 KO mice, which cannot synthesize AA in vivo. After a 4-week experimental period, mice were divided into six groups. The following three groups comprised the ethanol treatment groups: WT-E group (wild-type), KV-E group (AA-supplemented), and kt-E group (AA-deficient). Mice were exposed to an acute dose of ethanol (6 g ethanol/kg) administered by gavage once a day for three days. The other three control groups, namely, WT-C, KV-C, and kt-C control groups, received an equal volume of water via oral administration. Analysis of changes in body weight showed that mice in the kt-E group had significant loss of body weight compared to the control, KV-E, and WT-E groups. Behavioral analysis revealed that alcohol exposure significantly increased alcohol sensitivity in the kt-E group, whereas the WT-E, KV-E, and control groups developed ethanol tolerance. Aspartate transaminase (AST) levels in the kt-E group were significantly higher than those in the control, KV-E, and WT-E groups. The number of large and binucleated hepatocytes was significantly higher in the kt-E group than in the KV-E and WT-E groups. In addition, cytochrome P450 2E1 (CYP2E1) was over expressed in the central vein in the kt-E group when compared to the KV-E and WT-E groups. Our current findings indicate that AA supplementation in SMP30 KO mice can alleviate alcohol-induced liver damage by down regulating CYP2E1 expression. These results suggest that reduced CYP2E1 expression is a novel mechanism responsible for AA-induced reduction of ethanol-mediated oxidative str

关键词： Ascorbic Acid Alcohol Liver SMP30

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花叶病大蒜的茎端组织脱毒培养

华南农业大学学报 1987年第4期 1-8页

作者：赵顺庆李鹏飞范怀忠郭碧霞华南农业大学植保系华南农业大学园艺系华南农业大学植保系华南农业大学园艺系中华人民共和国江门动植物检疫所

本文论述了用MS(1962)培养基添加吲哚乙酸(IAA)、细胞激动素(kt)、萘乙酸(NAA)、2,4—二氯苯氧乙酸(2,4—D)、6—苄基嘌呤(BA)等生长调节物质,对开平金山火蒜及广州硬尾骨蒜两品种茎端0.100～0.125mm长的分生组织进行培养,获得了无毒种... 详细信息

本文论述了用MS(1962)培养基添加吲哚乙酸(IAA)、细胞激动素(kt)、萘乙酸(NAA)、2,4—二氯苯氧乙酸(2,4—D)、6—苄基嘌呤(BA)等生长调节物质,对开平金山火蒜及广州硬尾骨蒜两品种茎端0.100～0.125mm长的分生组织进行培养,获得了无毒种蒜,并提出了适于此两种大蒜无毒苗培养的培养基配方、生长调节质物的组合和浓度、以及光温条件等一套方案;论证了2,4—D、NAA、BA、光照和温度对愈伤组织诱导、芽分化和球茎形成上的作用,并对大蒜不同组织部位的培养作了初步的探讨。

关键词：大蒜花叶病大蒜花叶病毒茎端组织培养脱毒组织培养

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铝-镁合金的高温变形机构

物理学报 1965年第9期 1681-1688页

作者：王其闵王遂福窦仲伟郭懋端中国科学院浙江大学上海科技大学

用变温变速的拉伸试验,研究了Al-3％Mg和Al-6％Mg合金的高温变形行为,求得各温度下的激活能、激活体积和频率因子等。证明在250—400℃温度范围内,铝-镁合金变形机构可能是带割阶的螺位错作非保守性运动,变形方程为 ε=Nι_jb~2zAVexp{-... 详细信息

用变温变速的拉伸试验,研究了Al-3％Mg和Al-6％Mg合金的高温变形行为,求得各温度下的激活能、激活体积和频率因子等。证明在250—400℃温度范围内,铝-镁合金变形机构可能是带割阶的螺位错作非保守性运动,变形方程为 ε=Nι_jb~2zAVexp{-(△H_s-ι_jb~2τ/kt}。然后计算得到两种合金在不同温度下的割阶间距ι_j,运动位错密度ρ=Nι_j,割阶密度N以及位错速度等数值。

关键词：高温变形变形机构变温位错密度激活能活化能

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不同烟草种类的水分吸收与解吸特征

中国烟草学报 2006年第4期12卷 11-11页

作者： Han-Joo chung Gee-Chul Jang Yong-Ok Kim 吴晓芸 kt&G中央研究院Daejeon

烟草的水分含量会影响到其物理性能（如：填充力，优良指标等）、可燃性、烟气成分以及评吸的感官特性等。我们近期针对国产及进口烟叶的水分吸收及解吸特征开展了调查。试验在不同的温度条件（5，15，25，40℃）下及不同的相对湿度范... 详细信息

烟草的水分含量会影响到其物理性能（如：填充力，优良指标等）、可燃性、烟气成分以及评吸的感官特性等。我们近期针对国产及进口烟叶的水分吸收及解吸特征开展了调查。试验在不同的温度条件（5，15，25，40℃）下及不同的相对湿度范围内（11％～84％），由饱和盐溶液控制。根据不同的相对湿度，温度和烟叶种类，用获得的回归方程来预示平衡水分含量。该回归方程显示出高R^2（高于0．95），并预报出精确的平衡水分含量。[第一段]

关键词：解吸特征水分吸收种类烟草水分含量温度条件相对湿度回归方程

Feasibility and physics potential of detecting ^(8)B solar neutrinos at JUNO

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Chinese Physics C 2021年第2期45卷 93-110页

作者： Angel Abusleme Thomas Adam Shakeel Ahmad Sebastiano Aiello Muhammad Akram Nawab Ali Fengpeng An Guangpeng An Qi An Giuseppe Andronico Nikolay Anfimov Vito Antonelli Tatiana Antoshkina Burin Asavapibhop João Pedro Athayde Marcondes de André Didier Auguste Andrej Babic Wander Baldini Andrea Barresi Eric Baussan Marco Bellato Antonio Bergnoli Enrico Bernieri David Biare Thilo Birkenfeld Sylvie Blin David Blum Simon Blyth Anastasia Bolshakova Mathieu Bongrand Clément Bordereau Dominique Breton Augusto Brigatti Riccardo Brugnera Riccardo Bruno Antonio Budano Max Buesken Mario Buscemi Jose Busto Ilya Butorov Anatael Cabrera Hao Cai Xiao Cai Yanke Cai Zhiyan Cai Antonio Cammi Agustin Campeny Chuanya Cao Guofu Cao Jun Cao Rossella Caruso Cédric Cerna Jinfan Chang Yun Chang Pingping Chen Po-An Chen Shaomin Chen Shenjian Chen Xurong Chen Yi-Wen Chen Yixue Chen Yu Chen Zhang Chen Jie Cheng Yaping Cheng Alexander Chepurnov Davide Chiesa Pietro Chimenti Artem Chukanov Anna Chuvashova Gérard Claverie Catia Clementi Barbara Clerbaux Selma Conforti Di Lorenzo Daniele Corti Salvatore Costa Flavio Dal Corso Christophe De La Taille Jiawei Deng Zhi Deng Ziyan Deng Wilfried Depnering Marco Diaz Xuefeng Ding Yayun Ding Bayu Dirgantara Sergey Dmitrievsky Tadeas Dohnal Georgy Donchenko Jianmeng Dong Damien Dornic Evgeny Doroshkevich Marcos Dracos Frédéric Druillole Shuxian Du Stefano Dusini Martin Dvorak Timo Enqvist Heike Enzmann Andrea Fabbri Lukas Fajt Donghua Fan Lei Fan Can Fang Jian Fang Marco Fargetta Anna Fatkina Dmitry Fedoseev Vladko Fekete Li-Cheng Feng Qichun Feng Richard Ford Andrey Formozov Amélie Fournier Haonan Gan Feng Gao Alberto Garfagnini Alexandre Göttel Christoph Genster Marco Giammarchi Agnese Giaz Nunzio Giudice Franco Giuliani Maxim Gonchar Guanghua Gong Hui Gong Oleg Gorchakov Yuri Gornushkin Marco Grassi Christian Grewing Maxim Gromov Vasily Gromov Minghao Gu Xiaofei Gu Yu Gu Mengyun Guan Nunzio Guardone Maria Gul Cong Guo Jingyuan Guo Wanlei Guo Xinheng Guo Yuhang Guo Paul Hackspacher Caren Hagner Ran Han Yan Yerevan Physics Institute YerevanArmenia UniversitéLibre de Bruxelles BrusselsBelgium Universidade Estadual de Londrina LondrinaBrazil Pontificia Universidade Catolica do Rio de Janeiro RioBrazil Pontificia Universidad Católica de Chile SantiagoChile Universidad Tecnica Federico Santa Maria ValparaisoChile Beijing Institute of Spacecraft Environment Engineering BeijingChina Beijing Normal University BeijingChina China Institute of Atomic Energy BeijingChina Institute of High Energy Physics BeijingChina North China Electric Power University BeijingChina School of Physics Peking UniversityBeijingChina Tsinghua University BeijingChina University of Chinese Academy of Sciences BeijingChina Jilin University ChangchunChina College of Electronic Science and Engineering National University of Defense TechnologyChangshaChina Chongqing University ChongqingChina Dongguan University of Technology DongguanChina Jinan University GuangzhouChina Sun Yat-Sen University GuangzhouChina Harbin Institute of Technology HarbinChina University of Science and Technology of China HefeiChina The Radiochemistry and Nuclear Chemistry Group in University of South China HengyangChina Wuyi University JiangmenChina Shandong University JinanChina Institute of Modern Physics Chinese Academy of SciencesLanzhouChina Nanjing University NanjingChina Guangxi University NanningChina East China University of Science and Technology ShanghaiChina School of Physics and Astronomy Shanghai Jiao Tong UniversityShanghaiChina Tsung-Dao Lee Institute Shanghai Jiao Tong UniversityShanghaiChina Institute of Hydrogeology and Environmental Geology Chinese Academy of Geological SciencesShijiazhuangChina Nankai University TianjinChina Wuhan University WuhanChina Xi’an Jiaotong University Xi’anChina Xiamen University XiamenChina School of Physics and Microelectronics Zhengzhou UniversityZhengzhouChina Institute of Physics National Chiao-Tung University Hsinchu National United University Miao-Li Department of Physics National T

The Jiangmen Underground Neutrino Observatory(JUNO)features a 20 kt multi-purpose underground liquid scintillator sphere as its main *** of JUNO's features make it an excellent location for^8B solar neutrino measurements,such as its low-energy threshold,high energy resolution compared with water Cherenkov detectors,and much larger target mass compared with previous liquid scintillator *** this paper,we present a comprehensive assessment of JUNO's potential for detecting^8B solar neutrinos via the neutrino-electron elastic scattering process.A reduced 2 MeV threshold for the recoil electron energy is found to be achievable,assuming that the intrinsic radioactive background^(238)U and^(232)Th in the liquid scintillator can be controlled to 10^(-17)g/*** ten years of data acquisition,approximately 60,000 signal and 30,000 background events are *** large sample will enable an examination of the distortion of the recoil electron spectrum that is dominated by the neutrino flavor transformation in the dense solar matter,which will shed new light on the inconsistency between the measured electron spectra and the predictions of the standard three-flavor neutrino oscillation *** m^(2)_(21)=4.8times10^(-5);(7.5times10^(-5))eV^(2),JUNO can provide evidence of neutrino oscillation in the Earth at approximately the 3sigma(2sigma)level by measuring the non-zero signal rate variation with respect to the solar zenith ***,JUNO can simultaneously measureDelta m^2_(21)using^8B solar neutrinos to a precision of 20% or better,depending on the central value,and to sub-percent precision using reactor antineutrinos.A comparison of these two measurements from the same detector will help understand the current mild inconsistency between the value of Delta m^2_(21)reported by solar neutrino experiments and the KamLAND experiment.

关键词： neutrino oscillation solar neutrino JUNO