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A rapid, simple, and high-throughput UPLC-MS/MS method for simultaneous determination of bioactive constituents in Salvia miltiorrhiza with positive/negative ionization switching.

A rapid, simple, and high-throughput UPLC-MS/MS method for s...

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2018年中国质谱学术大会（CMSC2018）

作者： Hung-Yu Lin Ting-Sian Lin han-Ju Chien Yu-Min Juang Chao-Jung Chen Chang-Shen Wang Chien-Chen Lai Institute of Molecular Biology Chung Hsing University Waters Corporation No.90 Jianguo North RoadZhongshan District Proteomics Core Lab Department of Medical ResearchChinaMedical University Hospital Department of Agronomy Chung Hsing University

The dried root of Danshen(Salvia miltiorrhiza) is a Chinese medicine that has been used in traditional treatments for more than 2000 years in Asia. It has also been used to treat cardiovascular diseases for decades. The goal of this study was to develop a new, fast, and simple ultrahigh-performance liquid chromatography tandem mass spectrometry(UPLC–MS/MS) analytical method for the analysis of phenolic acids and tanshinones in Danshen. The samples were separated on a Kinetex F5 column(2.1?x?100?mm, 1.7?μm, pentafluorophenyl) using 78% acetonitrile with 0.1% formic acid as the mobile phase with isocratic elution while the flow rate was set at 0.45?mL/min. The analysis was performed in a Waters Xevo TQ mass spectrometer via multiple reaction monitoring(MRM) in polarity switching mode. The linearity values of the calibration curves of eight compounds were in the range from 3 to 3200?ng/mL(r2 > 0.99). Method validation was performed in terms of linearity, the relative standard deviations(RSDs) of the intra-day and inter-day, and it was found that precisions were within 15% and the accuracy ranged from 93% to 118%. The recovery was more than 87%. The limit of detection(LOD) and limit of quantification(LOQ) values of the eight analytes varied between 0.023 to 0.75?ng/mL and 0.375 to 1.5?ng/mL, respectively. This approach has the shortest analysis time for the separation of phenolic acids and tanshinones in Danshen using a UPLC core-shell column with F5 stationary phase. Meanwhile, this robust and high-throughput method not only has a short run time(2?min) but also reduce the consumption of organic solvents.

关键词： Salvia miltiorrhiza Tanshinones Phenolic acids UPLC-MS/MS

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STUDIES ON A NEW PATHOGENIC LUNG FLUKE——PARAGONIMUS HUEIT’UNGENSIS ***.

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Science in China,Ser.A 1975年第6期 785-814页

作者：钟惠澜许炽熛贺联印高佩芝邵兰丘福禧毕维德刘广汉欧阳正平申伯光易建中姚祖昇 Tropical Medicine Research Laboratory Peking Friendship Hospital Peking Department of Medieine Peking Medical College Affiliated People’s Hospital Hueit’ung County Epidemic Prevention Station Ch’ienyang Area Hunan Province

This paper records the discovery of a new pathogenic lung fluke Paragonimus huei-t’ungensis sp. nov. in Hueit’ung County of Hunan Province. The salient features of theadults of this lung fluke, its first and second intermediate hosts, rediae, cercariae, meta-cercariae and the results of feeding experiments on laboratory animals (albino rats, cats,and dogs) are described and compared with the relevant species of lung flukes alreadyknown. Biopsies of the migratory subcutaneous nodules done in 2 pediatric cases seen inthe endemic area of Hueit’ung County have both shown the presence of immature ado-lescent lung flukes, whose morphological features agree in general with those obtainedthrough feeding experiments in laboratory animals. But the reproductive organs of thesebiopsied adolescent flukes are poorly developed; and they do not produce eggs in thehuman body where they appear to migrate blindly from one place to another, indicatingthat man is not a suitable final host for this parasite, in spite of its pathogenic impli-cations. Hence one has to be careful not to confuse clinical cases of this new form ofparagonimiasis with those due to paragonimiasis szech’uanensis. Studies on the differentaspects of this new lung fluke are obviously of practical importance in the early diag-nosis, treatment, and prevention of the disease in the localities concerned.

关键词： UNGENSIS SP.NOV PARAGONIMUS HUEIT STUDIES ON A NEW PATHOGENIC LUNG FLUKE SP

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选择性绿激光前列腺汽化术与经尿道前列腺电切术对前列腺组织和梗阻严重程度影响的对比研究

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临床泌尿外科杂志 2007年第9期22卷 719-719页

作者： han D H chung J W Kim J Y 付毅博陈志敏不详奇致激光技术（中国）有限公司武汉430074

2004年7月间,研究者对比了80 W KTP绿激光前列腺汽化术(PVP)和经尿道前列腺电切术(TURP)在去除腺体组织与改善膀胱出口梗阻方面的临床疗效.

关键词：经尿道前列腺电切术膀胱出口梗阻前列腺汽化术绿激光前列腺组织 IPSS 逼尿肌功能神经性疾病

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长期热处理引起无毛鼠皮肤形成皱纹和氧化性损伤

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热处理技术与装备 2012年第6期33卷 63-63页

作者： Mi Hee Shin Jo -Eun Seo Yeon Kyung Kim Kyu han Kim Jin Ho chung

前面我们已经指出热振动能够引起皮肤细胞中母体金属蛋白酶的出现，这些结果也关系到长期热处理引起皮肤起皱纹。最近的研究中，我们检查到长期热处理（43℃，30min，3次／周，6周）在无毛鼠的皮肤形起皱纹。我们发现无毛鼠的皮肤经6周... 详细信息

前面我们已经指出热振动能够引起皮肤细胞中母体金属蛋白酶的出现，这些结果也关系到长期热处理引起皮肤起皱纹。最近的研究中，我们检查到长期热处理（43℃，30min，3次／周，6周）在无毛鼠的皮肤形起皱纹。我们发现无毛鼠的皮肤经6周一个周期反复热处理后引起皮肤起皱纹。在组织结构上无毛鼠的皮肤经热处理导致MMP-13蛋白质和mRNA的出现明显增加，但不是MMP-2和-9。我们还要指出长期热处理会降低抗氧化剂（过氧化氢酶）和过氧化物歧化酶（SOD）。另外，承受长期热振动后在老鼠皮肤上增加氧化性损伤。

关键词：皮肤细胞氧化性损伤热处理老鼠皱纹无毛过氧化物歧化酶 MMP-13

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Short-term overlap lamivudine treatment with adefovir dipivoxil in patients with lamivudine-resistant chronic hepatitis B

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World Journal of Gastroenterology 2008年第11期14卷 1781-1784页

作者： Soon Woo Nam Si Hyun Bae Seung Woo Lee Yeon Soo Kim Sang Bum Kang Jong Young Choi Se Hyun Cho Seung Kew Yoon Joon-Yeol han Jin Mo Yang Young Suk Lee Division of Gastroenterology and Hepatology Department ofInternal medicineMedical College of the Catholic University ofKoreaDaejeon St.Mary's Hospital#62 Daeheung-dongChung-guDaejeonRepublic of Korea

AIM:To evaluate the efficacy of short-term overlap lamivudine therapy with adefovir in patients with lamivudine-resistant and nave chronic hepatitis B,we compared patients receiving overlap therapy with those receiving adefovir alone. METHODS:Eighty patients who had received lamivudine treatment for various periods and had a lamivudine- resistant liver function abnormality were *** of these patients received adefovir treatment combined with lamivudine treatment for≥2 mo,while the other 40 received adefovir *** assessed the levels of hepatitis B virus(HBV)DNA at 0,12 and 48 wk and serum alanine aminotransferase(ALT)levels after 0,12, 24 and 48 wk of adefovir treatment in each group. RESULTS:We found serum ALT became normalized in 72(87.5%)of the 80 patients,and HBV DNA decreased by≥2 log10 copies/mL in 60(75%)of the 80 patients at the end of a 48-wk *** DNA levels were not significantly different between the *** improvements in serum ALT were also not significantly different between the two groups. CONCLUSION:These findings suggest short-term overlap lamivudine treatment results in no better virological and biological outcomes than non-overlap adefovir monotherapy.

关键词：慢性乙型肝炎乙肝病毒短期治疗抵抗力

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Verification of gene expression profiles for colorectal cancer using 12 internet public microarray datasets

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World Journal of Gastroenterology 2014年第46期20卷 17476-17482页

作者： Yu-Tien Chang chung-Tay Yao Sui-Lung Su Yu-Ching Chou Chi-Ming Chu Chi-Shuan Huang Harn-Jing Terng Hsiu-Ling Chou Thomas Wetter Kang-Hua Chen Chi-Wen Chang Yun-Wen Shih Ching-Huang Lai Division of Biomedical Statistics and Informatics School of Public HealthNational Defense Medical Center Department of Emergency Cathay General Hospital Department of Epidemiology School of Public HealthNational Defense Medical Center Division of Colorectal Surgery Cheng Hsin Rehabilitation Medical Center Advpharma Inc. Department of Nursing Far Eastern Memorial Hospital Oriental Institute of Technology Department of Medical Informatics Faculty of MedicineUniversity of Heidelberg69120 HeidelbergGermany School of Nursing College of MedicineChang Gung University

AIM:To verify gene expression profiles for colorectal cancer using 12 internet public microarray ***:Logistic regression analysis was performed,and odds ratios for each gene were determined between colorectal cancer(CRC)and *** microarray datasets of GSE 4107,4183,8671,9348,10961,13067,13294,13471,14333,15960,17538,and 18105,which included 519 cases of adenocarcinoma and 88 normal mucosa controls,were pooled and used to verify 17 selective genes from 3 published studies and estimate the external ***:We validated the 17 CRC-associated genes from studies by Chang et al(Model 1:5 genes),Marshall et al(Model 2:7 genes)and han et al(Model 3:5genes)and performed the multivariate logistic regression analysis using the pooled 12 public microarray datasets as well as the external *** goodnessof-fit test of Hosmer-Lemeshow(H-L)showed statistical significance(P=0.044)for Model 2 of Marshall et al in which observed event rates did not match expected event rates in subgroups of the model *** and observed event rates in subgroups were similar,which are called well calibrated,in Models 1,3and 4 with non-significant P values of 0.460,0.194 and1.000 for H-L tests,respectively.A 7-gene model of CPEB4,EIF2S3,MGC20553,MS4A1,ANXA3,TNFAIP6and IL2RB was pairwise selected,which showed the best results in logistic regression analysis(H-L P=1.000,R2=0.951,areas under the curve=0.999,accuracy=0.968,specificity=0.966 and sensitivity=0.994).CONCLUSION:A novel gene expression profile was associated with CRC and can potentially be applied to blood-based detection assays.

关键词： Gene expression profiles Colorectal cancer Microar

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通过60比特24深度量子随机线路采样实现量子计算优势性

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Science Bulletin 2022年第3期67卷 240-245页

作者：朱庆玲曹思睿陈福生陈明城陈厦微钟东勳邓辉杜亚杰范道金龚明郭成郭楚郭少俊韩廉琛洪林音黄合良霍永恒李丽萍李娜李少炜李渊梁福田林春林金钱浩然乔丹荣皓苏红孙丽华王梁媛王石宇吴大超吴玉林徐昱严凯杨威风杨洋叶杨森尹江涵应翀余家乐查辰张查张海滨张凯丽张一鸣赵涵赵有为周亮陆朝阳彭承志朱晓波潘建伟 Hefei National Laboratory for Physical Sciences at the Microscale and Department of Modern Physics University of Science and Technology of ChinaHefei 230026China Shanghai Branch CAS Center for Excellence in Quantum Information and Quantum PhysicsUniversity of Science and Technology of ChinaShanghai 201315China Shanghai Research Center for Quantum Sciences Shanghai 201315China QuantumCTek Co. Ltd.Hefei 230026China Henan Key Laboratory of Quantum Information and Cryptography Zhengzhou 450000China Shanghai Institute of Technical Physics Chinese Academy of SciencesShanghai 200083China

高精度地扩展量子比特规模是实现实用量子计算必须克服的基础难题.本文将祖冲之2.0升级至祖冲之2.1,该处理器的基本量子操控保真度分别为:单比特门99.84%,两比特1门99.40%,读取97.74%,相比2.0系统在读取上得到了巨大的提升为了表征系统... 详细信息

高精度地扩展量子比特规模是实现实用量子计算必须克服的基础难题.本文将祖冲之2.0升级至祖冲之2.1,该处理器的基本量子操控保真度分别为:单比特门99.84%,两比特1门99.40%,读取97.74%,相比2.0系统在读取上得到了巨大的提升为了表征系统的整体性能,本文通过执行随机量子电路采样任务(-种量子霸权协议)进行基准测试.实验结果表明,可以实现60比特24深度的随机量子线路采样,线路最终保真度为(0.0366土0.00345)%.该线路的经典模拟复杂度比此前的祖冲之2.0量子霸权线路(55比特20深度)难5000倍,因此可以说,本研究在量子随机线路采样问题上进一步增强了量子计算的算力优势.

关键词：量子计算表征系统基准测试量子电路保真度单比特祖冲之量子线路

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永续传承古城瑰宝展现江南文化魅力——顶级专家为苏州历史文化名城保护“会诊把脉”

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建筑实践 2022年第8期5卷 168-187页

作者：杨保军周岚阮仪三刘太格伍江常青段进王凯李晓江张泉韩冬青贝建中叶斌杨新海张松贺云翱吴永发中国城市规划学会住房和城乡建设部江苏省住房和城乡建设厅同济大学国家历史文化名城研究中心新加坡国家艺术理事会新加坡大学建筑系咨询委员会法国建筑科学院中国科学院中国建筑学会同济大学建筑与城市规划学院东南大学中国城市规划设计研究院中国建筑学会建筑师分会东南大学建筑设计研究院东南大学建筑学院贝氏建筑事务所南京市自然资源和规划局同济大学城市规划系南京大学历史系南京大学文化与自然遗产研究所中国历史文化名城苏州研究院苏州大学建筑学院不详

2022年2月9日,由苏州市委、市政府主办,苏州国家历史文化名城保护区、姑苏区、苏州市自然资源和规划局承办的苏州国家历史文化名城保护专家咨询会在苏州市会议中心举行,17位国内外相关领域顶级专家齐聚一堂,结合各自的专业方向和研究课... 详细信息

2022年2月9日,由苏州市委、市政府主办,苏州国家历史文化名城保护区、姑苏区、苏州市自然资源和规划局承办的苏州国家历史文化名城保护专家咨询会在苏州市会议中心举行,17位国内外相关领域顶级专家齐聚一堂,结合各自的专业方向和研究课题发表真知灼见,为苏州历史文化名城保护“会诊把脉”,为古城保护更新开启新思路。

关键词：历史文化名城保护文化魅力

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褐煤干燥后水分复吸规律及平衡含水率预测

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煤炭学报 2015年第11期40卷 2661-2667页

作者：刘英鹏杨晴胡敏杨海平王贤华陈汉平华中科技大学煤燃烧国家重点实验室湖北武汉430074

为研究干燥温度及环境温湿度对我国褐煤干燥后水分复吸的影响,通过恒温恒湿箱对经过不同干燥温度(80,120,160℃)处理后的海拉尔褐煤、云南褐煤、霍林河褐煤进行不同相对湿度(50%,70%,90%)、不同吸湿温度(20,30,40℃)条件下的水分复吸实... 详细信息

为研究干燥温度及环境温湿度对我国褐煤干燥后水分复吸的影响,通过恒温恒湿箱对经过不同干燥温度(80,120,160℃)处理后的海拉尔褐煤、云南褐煤、霍林河褐煤进行不同相对湿度(50%,70%,90%)、不同吸湿温度(20,30,40℃)条件下的水分复吸实验。研究表明,相对湿度对褐煤平衡含水率影响最大。随着吸湿温度升高,褐煤平衡含水率先增加后减小,不同于以往认为的一直减小的规律。通过饱和溶液法进一步检测了褐煤在相对湿度0~100%范围内等温条件下水分复吸的平衡含水率,发现其随相对湿度增加呈'S'型曲线变化,chung-Pfost模型能够很好地预测褐煤水分复吸的平衡含水率。

关键词：褐煤水分复吸平衡含水率干燥温度环境温度相对湿度

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术前肌电图和脑干听觉诱发电位与面肌痉挛微血管减压术后迟发性面瘫发生的关系(英文)

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中华神经外科疾病研究杂志 2012年第4期11卷 326-326页

作者： Kim HJ Park YS Ryu JS Huh R han I Shin DA Kim TG Cho KG chung SS Department of Neurosurgery Bundang CHA Medical CenterCHA University

Background Delayed facial palsy ( DFP) after microvascular decompression ( MVD) in patients with hemifacial spasm ( HFS) is not uncommon,but the cause remains unknown. Objectives To assess whether intraoperative electromyography ( EMG) and brainstem auditory evoked potential ( BAEP) can predict DFP after MVD. Methods Between September 2009 and February 2011 we examined 86 patients,9 of whom ( 10. 4% ) developed DFP after MVD on the same side. All patients underwent MVD and were followed - up for a median period of 13 months ( range 6-22) . We retrospectively examined intraoperative facial EMG and BAEP findings using our MVD patients' registry. We excluded secondary HFS and immediate postoperative facial palsy after MVD in this study. We assessed the prevalence and clinical characteristics of DFP and compared EMG and BAEP findings between DFP and non-DFP groups. Results: All pa- tients recovered completely,with a mean time to recovery of 37. 8 days ( range 22-57) . There were no significant differences between DFP and non - DFP patients in terms of the amplitude and latency of intraoperative EMG and BAEP. Conclusion The usefulness of intraoperative facial EMG and BAEP is limited and cannot predict DFP after MVD for HFS. We speculate that DFP after MVD is not associated with permanent nerve damage according to the EMG findings.

关键词：术前肌电图脑干听觉面肌痉挛微血管减压术

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