检索结果-南通市图书馆

Construction of a predictive model for acute liver failure after hepatectomy based on neutrophil-to-lymphocyte ratio and albuminbilirubin score

引用

World Journal of Gastrointestinal Surgery 2024年第4期16卷 1087-1096页

作者： Xiao-Pei Li Zeng-Tao Bao Li Wang chun-yan zhang Wen yang Department of Family Planning and Assisted Reproductive Technology The First People’s Hospital of LianyungangLianyungang 222000Jiangsu ProvinceChina Department of Gastrointestinal Surgery The First People’s Hospital of LianyungangLianyungang 222000Jiangsu ProvinceChina Department of Laboratory Medicine The First People’s Hospital of LianyungangLianyungang 222000Jiangsu ProvinceChina Department of Gynecology The First People’s Hospital of LianyungangLianyungang 222000Jiangsu ProvinceChina

BACKGROUND Acute liver failure(ALF)is a common cause of postoperative death in patients with hepatocellular carcinoma(HCC)and is a serious threat to patient *** neutrophil-to-lymphocyte ratio(NLR)is a common inflammatory indicator that is associated with the prognosis of various diseases,and the albumin-bilirubin score(ALBI)is used to evaluate liver function in liver cancer ***,this study aimed to construct a predictive model for postoperative ALF in HCC tumor integrity resection(R0)based on the NLR and ALBI,providing a basis for clinicians to choose appropriate treatment *** To construct an ALF prediction model after R0 surgery for HCC based on NLR and *** In total,194 patients with HCC who visited The First People’s Hospital of Lianyungang to receive R0 between May 2018 and May 2023 were enrolled and divided into the ALF and non-ALF *** compared differences in the NLR and ALBI between the two *** risk factors of ALF after R0 surgery for HCC were screened in the univariate *** risk factors were analyzed by multifactorial logistic *** then constructed a prediction model of ALF after R0 surgery for HCC.A receiver operating characteristic curve,calibration curve,and decision curve analysis(DCA)were used to evaluate the value of the prediction *** Among 194 patients with HCC who met the standard inclusion criteria,46 cases of ALF occurred after R0(23.71%).There were significant differences in the NLR and ALBI between the two groups(P<0.05).The univariate analysis showed that alpha-fetoprotein(AFP)and blood loss volume(BLV)were significantly higher in the ALF group compared with the non-ALF group(P<0.05).The multifactorial analysis showed that NLR,ALBI,AFP,and BLV were independent risk factors for ALF after R0 surgery in *** predictive efficacy of NLR,ALBI,AFP,and BLV in predicting the occurrence of ALT after R0 surgery for HCC was average[area under the curve(AUC)NLR=0.767,AUCALB

关键词： Acute liver failure Hepatocellular carcinoma Hepatectomy Neutrophil-to-lymphocyte ratio Albumin-bilirubin score

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fusion Wheat Histone H4 Protein Increases Transfection Efficiency of Non-viral DNA Vector

引用

Chemical Research in Chinese Universities 2011年第2期27卷 264-268页

作者： WANG chun-yan zhang Yu-jing College of Animal Science and Veterinary Medicine Jilin University Changehun 130062 P. R. China

The lack of efficient and non-toxic gene delivery, preferably with non-viral DNA vectors, is generally regarded as a major limitation for gene therapy. In this study, a wheat histone H4 gene was cloned from Triticum aestivum, sequenced, modified and expressed in E. coli. The wheat histone H4 gene and reconstructed H4TL gene encoded wheat histone H4 and a recombinant protein of 141 amino acids with an approximate molecular weight of 15500. Gel electrophoresis mobility shift assays demonstrated that the purified protein had high affinity for DNA. Most significantly, the complex of plasmid pEGFP/C1 with H4TL was transfected with increased efficiency into MCF-7, HO8910, LNCap, A549 and HeLa cells in vitro. These results demonstrate that the targeting of non-viral vectors to tumor-specific receptors provides a cheap, simple and highly efficient tool for gene delivery.

关键词： Wheat histone H4 Non-viral gene delivery Histonefection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Risk factors for Clostridioides difficile infection in children and adolescents with inflammatory bowel disease: a systematic review and meta-analysis

引用

World Journal of Pediatrics 2022年第1期18卷 27-36页

作者： Sheng-Bo Fang yan-Qing Song chun-yan zhang Li-Bo Wang Department of Pharmacy First Hospital of Jilin UniversityChangchunChina Department of Pediatric Gastroenterology Unit First Hospital of Jilin University71 Xinmin StreetChangchun130021China

Background Risk factors and consequences associated with Clostridioides difficile infection(CDI)in children and adolescents with inflammatory bowel disease(IBD)are still *** conduct a systematic review and meta-analysis to assess risk factors and outcomes associated with CDI in children and adolescents with *** PubMed,EMBASE and Cochrane Library databases were searched from inception to 24th February,*** investigating risk factors,bowel surgery rate in pediatric IBD patients with and without CDI were ***-effects model was used for calculating summary ***–Ottawa scale(NOS)was used for quality *** Fourteen studies,comprising 17,114 patients,were *** was a significant association between 5-aminosalicylic acid(5-ASA)use and CDI[odds ratio(OR)=1.95,95%confidence interval(CI)1.26–3.03],with minimal heterogeneity(I^(2)=0.00%).Increased risk of active disease(OR=4.66,95%CI 2.16–10.07)were associated with CDI in those studies performed in high quality score(NOS>6)and significantly higher CDI rates in studies conducted outside USA(OR=2.94,95%CI 1.57–5.58).The bowel surgery rate in IBD with CDI was 3.8–57.1%,compared to that in IBD without CDI(0–21.3%).All studies were of moderate to high *** 5-ASA use and active disease might be risk factors associated with CDI in children and adolescents with *** surgery rates associated with CDI in IBD patients varied ***-scale clinical studies on CDI in children and adolescents with IBD are still needed to verify risk factors and outcomes.

关键词： Adolescent Children Clostridioides difficile infection Inflammatory bowel disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Substitution of a Conserved Glycine in the PHR Domain of Arabidopsis CRYPTOCHROME 1 Confers a Constitutive Light Response

引用

Molecular Plant 2012年第1期5卷 85-97页

作者： Nan-Nan Gu yan-chun zhang Hong-Quan yang National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200032China Plant Science Institute School of Agriculture and BiologyShanghai Jiaotong UniversityShanghai 200240China

CRYPTOCHROMES （CRYs） are photolyase-like ultraviolet-A/blue light photoreceptors that mediate various light responses in plants. The signaling mechanism of Arabidopsis CRYs （CRY1 and CRY2） involves direct CRY-COP1 interaction. Here, we report that CRY1G380R, which carries a Gly-to-Arg substitution of the highly conserved G380 in the photo-lyase-related （PHR） domain of Arabidopsis CRY1, shows constitutive CRY1 photoreceptor activity in Arabidopsis. Transgenic plants overexpressing CRY1G380R display a constitutively photomorphogenic （COP） phenotype in darkness, as well as a dramatic early flowering phenotype under short-day light conditions （SD）. We further demonstrate that CRY1G380R expression driven by the native CRY1 promoter also results in a COP phenotype in darkness. Moreover, over- expression of either the Arabidopsis homolog CRY2G377R or the rice ortholog OsCRYlbG388R of CRY1G380R in Arabidopsis results in a COP phenotype in darkness. Cellular localization studies indicate that CRY1G380R co-localizes with COP1 in the same nuclear bodies （NBs） in vivo and inhibits the nuclear accumulation of COP1 in darkness. These results suggest that the conserved G380 may play a critical role in regulating the photoreceptor activity of plant CRYs and that CRY1G380R might constitutively phenocopy the photo-activated CRY1 in darkness and thus constitutively mediate CRY1 signaling.

关键词： Arabidopsis CRY1 CRy1G380R COP phenotype COP1.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

MicroRNA171c-Targeted SCL6-Ⅱ, SCL6-Ⅲ, and SCL6-Ⅳ Genes Regulate Shoot Branching in Arabidopsis

引用

Molecular Plant 2010年第5期3卷 794-806页

作者： Long Wang yan-Xia Mai yan-chun zhang Qian Luo Hong-Quan yang National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China School of Agriculture and Biology Shanghai Jiaotong University Shanghai 200240 China

MicroRNAs （miRNAs） are -21-nucleotide noncoding RNAs that play critical roles in regulating plant growth and development through directing the degradation of target mRNAs. Axillary meristem activity, and hence shoot branching, is influenced by a complicated network that involves phytohormones such as auxin, cytokinin, and strigolactone. GAI, RGA, and SCR （GRAS） family members take part in a variety of developmental processes, including axillary bud growth. Here, we show that the Arabidopsis thaliana microRNA171c （miR171c） acts to negatively regulate shoot branching through targeting GRAS gene family members SCARECROW-LIKE6-Ⅱ （SCL6-Ⅱ）, SCL6-Ⅲ, and SCL6-Ⅳ for cleavage. Transgenic plants overexpressing MIR171c （35Spro-MIR171c） and sd6-Ⅱ scl6-Ⅲ scl6-Ⅳ triple mutant plants exhibit a similar reduced shoot branching phenotype. Expression of any one of the miR171c-resistant versions of SCL6-Ⅱ, SCL6-Ⅲ, and SCL6-Ⅳ in 35Spro- MIR171c plants rescues the reduced shoot branching phenotype. Scl6-Ⅱ scl6-Ⅲ scl6-Ⅳ mutant plants exhibit pleiotropic phenotypes such as increased chlorophyll accumulation, decreased primary root elongation, and abnormal leaf and flower patterning. SCL6-Ⅱ, SCL6-Ⅲ, and SCL6-Ⅳ are located to the nucleus, and show transcriptional activation activity. Our results suggest that miR171c-targeted SCL6-Ⅱ, SCL6-Ⅲ, and SCL6-Ⅳ play an important role in the regulation of shoot branch production.

关键词： Arabidopsis thaliana axillary meristem miRNA171 SCL6 shoot branching.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Enzymatic synthesis of nucleosides by nucleoside phosphorylase co-expressed in Escherichia coli

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第11期11卷 880-888页

作者： Qing-bao DING Ling OU Dong-zhi WEI Xiao-kun WEI yan-mei XU chun-yan zhang State Key Laboratory ofBtoreactor Engmeering East Chma Untverstty of Science and Technology Shanghai 200237 China Shanghai Scibest Biotechnology Co. Ltd. Shanghai 200235 China

Nucleoside phosphorylase is an important enzyme involved in the biosynthesis of nucleosides. In this study, purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase were co-expressed in Escherichia coli and the intact cells were used as a catalyst for the biosynthesis of nucleosides. For protein induction, lactose was used in place of isopropyl β-D-l-thiogalactopyranoside （IPTG）. When the concentration of lactose was above 0.5 mmol/L, the ability to induce protein expression was similar to that of IPTG. We determined that the reaction conditions of four bacterial strains co-expressing these genes （TUD, TAD, DUD, and DAD） were similar for the biosyntheses of 2,6-diaminopurine nucleoside and 2,6-diaminopurine deoxynucleoside. When the substrate concentration was 30 mmol/L and 0.5% of the recombinant bacterial cell volume was used as the catalyst （pH 7.5）, a greater than 90% conversion yield was reached after a 2-h incubation at 50℃. In addition, several other nucleosides and nucleoside derivatives were efficiently synthesized using bacterial strains co-expressing these recombinant enzymes.

关键词： Nucleoside phosphorylase Lactose Enzymatic synthesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

引用

Chinese Medical Sciences Journal 2008年第3期23卷 162-165页

作者： chun-yan zhang Rong Rong Xiao-ying Wang Department of Radiology Peking University First Hospital Functional Imaging Center Advanced Academy of Interdisciplinary Sciences Peking University Beijing 100034

Objective To investigate the signal intensity and apparent diffusion coefficient (ADC) of bone marrow of normal adult man on diffusion weighted imaging (DWI). Methods Fifteen healthy volunteers and thirty-eight patients with benign prostatic hyperplasia or normal prostate were enrolled in this study, with age range 28-82 years old (mean 55.26 ± 18.05 years). All people were examined with large field DWI on a 3.0T magnetic resonance scanner, which ranges from the top of head to the lower limb. The signal-to-noise ratio (SNR) on the DWI and ADC of lumber vertebra at renal hilum level, left ilium and superior segment of left femur were measured. The measured SNR and ADC value of the above sites were compared by one way analysis of variance and their correlations with age were investigated by Pearson's correlation analysis. Results The SNR of lumber vertebra, left ilium and left femur showed no significant difference (F = 0.271, P = 0.763). The SNR of lumber vertebra (r = 0.309, P = 0.024) and left ilium (r = 0.359, P = 0.008) showed positive correlation with age, while the SNR of left femur showed no correlation with age (r = -0.163, P = 0.283). The ADC of lumber vertebra [(0.617 ± 0.177) ×10-3 mm2/s] was significantly higher than that of left ilium [(0.404 ± 0.112) ×10-3 mm2/s, P < 0.001] and left femur [(0.362 ± 0.092) ×10-3 mm2/s, P < 0.001], while the ADC of left ilium and left femur had no significant difference. The ADC of lumber vertebra, left ilium and left femur showed no correlation with age. Conclusion Understanding of age-related changes of normal adult bone marrow on DWI is very important to differentiate the normal bone marrow and abnormal lesions.

关键词： diffusion weighted imaging magnetic resonance imaging bone marrow

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synthesis and antitumor activity of nitric oxide releasing derivatives of AT1 antagonist

引用

Chinese Chemical Letters 2009年第3期20卷 302-305页

作者： yan chun zhang Jin Pei Zhou Xiao Ming Wu Wei Hong Pan Department of Medicinal Chemistry China Pharmaceutical University Nanjing 210009 China

A series of novel nitric oxide-donating derivatives （7a-e, 8a-e） were synthesized by coupling furoxan and nitric oxide with irbesartan analogue and their cytotoxicity against BEL7402 cells in vitro were evaluated by MTT method. It was found that 8c exhibits the most cytotoxic activities with ICso value of 12.5 μmol/L. The hybrids of AT1 antagonist and nitric oxide donor appear to have beneficial effects on antitumor.

关键词： AT1 antagonist Nitric oxide-donating Antitumor Synthesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

NORMAL APPEARANCE OF LARGE FIELD DIFFUSION WEIGHTED IMAGING ON 3.0T MRI

引用

Chinese Medical Sciences Journal 2008年第3期23卷 158-161页

作者： Rong Rong chun-yan zhang Xiao-ying Wang Department of Radiology Peking University First Hospital Functional Imaging Center Advanced Academy of Interdisciplinary Sciences Peking University Beijing 100034

Objective To evaluate the normal appearance of large field diffusion weighted imaging (DWI) on 3.0T magnetic resonance imaging (MRI). Methods Twenty healthy volunteers and thirty patients with benign prostate hyperplasia were included in this study. All patients were examined with large field DWI on 3.0T MRI. Normal tissue appearance was analyzed and apparent diffusion coefficient (ADC) of normal tissue with high signal intensity was measured. The ADC values of bilateral symmetrical tissue were also compared. The ADC values of intervertebral disks of healthy people younger than 50 years and exceeding 50 years were compared. Results Salivary gland, spleen, kidney, gallbladder, bladder, prostate, seminal vesicle, testis, intervertebral disk, liquid in articular cavity and lymph node showed high signal intensity on large field DWI, while lung, liver and bone showed hypo-signal intensity. The mean ADC values of partial hyperintensity tissue were as followed: parotid gland (1.088 ± 0.114) ×10-3 mm2/s, submaxillary gland (1.309 ± 0.189) ×10-3 mm2/s, kidney (1.909 ± 0.143) ×10-3 mm2/s, seminal vesicle (1.669 ± 0.168) ×10-3 mm2/s, testis (1.028 ± 0.075) ×10-3 mm2/s, spleen (0.963 ± 0.108) ×10-3 mm2/s, bladder (2.898 ± 0.267) ×10-3 mm2/s, prostate (1.448 ± 0.132) ×10-3 mm2/s, intervertebral disks (1.360 ± 0.140) ×10-3 mm2/s. No statistical significance was found between the ADC values of bilateral symmetrical tissues. The difference of ADC values of intervertebral disks of healthy people younger than 50 years [(1.372 ± 0.142) ×10-3 mm2/s] and exceeding 50 years [(1.344 ± 0.134) ×10-3 mm2/s] showed statistical significance (P = 0.040). Conclusion Understanding the high signal intensity of normal tissue on large field DWI may help to differentiate the normal tissues and abnormal ones.

关键词： diffusion weighted imaging magnetic resonance imaging normal appearance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

TLR2 signaling subpathways regulate TLR9 signaling for the effective induction of IL-12 upon stimulation by heat-killed Brucella abortus

引用

Cellular & Molecular Immunology 2012年第4期9卷 324-333页

作者： chun-yan zhang Nan Bai Zhu-Hong zhang Ning Liang Lan Dong Rong Xiang Cheng-Hu Liu Department of Immunology Medical School Nankai University Tianjin China

Brucella abortus is a Gram-negative intracellular bacterium that induces MyD88-dependent IL-12 production in dentritic cells （DCs） and a subsequent protective Thl immune response. Previous studies have shown that the Toll-like receptor 2 （TLR2） is required for tumor-necrosis factor （TNF） production, whereas TLR9 is responsible for IL-12 induction in DCs after exposure to heat-killed Brucella abortus（HKBA）. TLR2 is located on the cell surface and is required for optimal microorganism-induced phagocytosis by innate immune cells; thus, phagocytosis is an indispensable preliminary step for bacterial genomic DNA recognition by TLR9 in late-endosomal compartments. Here, we hypothesized that TLR2-triggered signals after HKBA stimulation might cross-regulate TLR9 signaling through the indirect modulation of the phagocytic function of DCs or the direct modulation of cytokine gene expression. Our results indicate that HKBA phagocytosis was TLR2-dependent and an essential step for IL-12p40 induction. In addition, HKBA exposure triggered the TLR2-mediated activation of both p38 and extracellular signal-regulated kinase 1/2 （ERKI/2）. Interestingly, although p38 was required for HKBA phagocytosis and phagosome maturation, ERK1/2 did not affect these processes but negatively regulated IL-12 production, Although p38 inhibitors tempered both TNF and IL.12 responses to HKBA, pre-treatment with an ERKI/2 inhibitor significantly increased IL-12p40 and abrogated TNF production in HKBA-stimulated DCs. Further experiments showed that the signaling events that mediated ERK1/2 activation after TLR2 triggering also required HKBA-induced Ras activation. Furthermore, Ras-guanine nucleotide-releasing protein 1 （RasGRP1） mediated the TLR2-induced ERKI/2 activation and inhibition of IL-12p40 production. Taken together, our results demonstrated that HKBA-mediated TLR2-triggering activates both the p38 and ERK1/2 signaling subpathways, which divergently regulate TLR9 activation at several levels to

关键词： ERK interleukin-12 phagocytosis p38 TLR2 TLR9 TNF

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：