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Identification of Two Novel Mitochondrial DNA Deletions Induced by Ionizing Radiation

Biomedical and Environmental Sciences 2012年第5期25卷 533-541页

作者： ZHAO xiao Tao FENG Jiang Bin LI Yu Wen LUO Qun YANG Xin chun LU Xue CHEN De Qing liu Qing Jie Department of Cardiology Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China Key Laboratory of Radiological Protection and Nuclear Emergency National Institute Jor Radiological Protection Chinese Center Jbr Disease Control and Prevention Beijing 100088 China Department of Transfusion Chinese PLA General Hospital Beijing 100853 China

Abstract Objective We identify ionizing radiation-induced mitochondrial DNA （mtDNA） deletions in human lymphocytes and their distribution in normal populations. Methods Long-range polymerase chain reactions （PCR） using two pairs of primers specific for the human mitochondrial genome were used to analyze the lymphoblastoid cell line following exposure to 10 Gy 6~Co y-rays. Limited-condition PCR, cloning and sequencing techniques were applied to verify the mtDNA deletions detected with long-range PCR. Human peripheral blood samples were irradiated with 0, 2 and 6 Gy ^60Co y-rays, and real-time PCR analysis was performed to validate the mtDNA deletions. In order to know the distribution of mtDNA deletions in normal population, 222 healthy Chinese adults were also investigated. Results Two mtDNA deletions, a 7455-bp deletion （nt475-nt7929 in heavy strand） and a 9225-bp deletion （nt7714 -nt369 in heavy strand）, occurring between two 8-bp direct repeats, were identified in lymphoblastoid cells using long-range PCR, limited-condition PCR and sequencing. These results were also observed for ^60Co y-rays irradiated human peripheral blood cells. Conclusion Two novel mtDNA deletions, a 7455-bp deletion and a 9225-bp deletion, were induced by ionizing radiation. The rate of the mtDNA deletions within a normal population was related to the donors＇ age, but was independent of gender.

关键词： Mitochondrial DNA deletion Ionizing radiation Lymphocytes Chinese adults

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Anti-proliferative and apoptotic effects of S1,a tetrandrine derivative,in human gastric cancer BGC-823 cells

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Chinese Journal of Natural Medicines 2016年第7期14卷 527-533页

作者： LEI Rong-Rong HU Hai-Feng BAI Fan liu Ying WU chun-Zhen HUANG xiao-Xing XIE Li-Ping HU You-Jia Shanghai Institute of Pharmaceutical Industry Shanghai 200040China Sinopharm Health Industry Research Co. Ltd.Shanghai 200040China The Kochi Prefectural Makino Botanical Garden Kochi 4200-6Japan

[ABSTRACT] The aim of the study was to investigate the anti-proliferation and apoptosis-inducing effects of S1, a novel tetrandrine derivative, in human gastric cancer BGC-823 cells and explore the possible mechanism of action. The anti-proliferative activity was determined by MTT assay; the induction of cell cycle arrest and apoptosis were detected by flow cytometry. Quantitative real time RT-PCR and Western blotting were used to evaluate the mRNA and protein expression levels in mitochondrial pathway. SI significantly reduced cell viability and induced a G2/M phase arrest and apoptosis in dose- and time-dependent manner. Further studies showed that S1 increased mRNA and protein expression of Bax and the Bax/Bcl-2 ratio. Moreover, S1 decreased the protein expression of procaspase-9 and procaspase-3, suggesting that the induction of apoptosis may be related to the alteration of the ratio of Bax/Bcl-2 and the activation of caspases. These findings suggested that S1 merits further investigation as a novel therapeutic agent for the treatment of human gastric cancer.

关键词： Tetrandrine derivative BGC-823 cells G2/M phase arrest Apoptosis

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叶面肥与竹炭有机肥对土壤-稻虾系统镉吸收的影响及其健康评价

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土壤通报 2024年第4期55卷 1099-1109页

作者：肖罗长倪才英黎衍亮刘鑫简敏菲江西师范大学地理与环境学院江西南昌330022 江西师范大学生命科学学院江西南昌330022

【目的】为探究不同修复材料对镉(Cd)污染稻虾种养系统的修复效果。【方法】本研究以南方稻虾田为对象,设计了叶面肥和竹炭有机肥及其组合共6组不同处理类型(对照CK,T1为叶面喷施硒肥,T2为叶面喷施L-半胱氨酸,T3为竹炭有机肥,T4为竹炭... 详细信息

【目的】为探究不同修复材料对镉(Cd)污染稻虾种养系统的修复效果。【方法】本研究以南方稻虾田为对象,设计了叶面肥和竹炭有机肥及其组合共6组不同处理类型(对照CK,T1为叶面喷施硒肥,T2为叶面喷施L-半胱氨酸,T3为竹炭有机肥,T4为竹炭有机肥+硒肥,T5为竹炭有机肥+L-半胱氨酸);测定土壤pH值、有机质、土壤不同形态Cd含量、水稻植株各部位以及小龙虾Cd含量;运用结合强度指数、迁移率指数、潜在生态风险指数和膳食风险指数分别对土壤、水稻各部位、小龙虾Cd污染风险进行评价。【结果】T3~T5处理土壤pH值、有机质较CK处理显著上升,上升幅度分别为5.71%~6.12%和3.28%~4.23%。DTPA提取态Cd含量处理显著下降,下降率分别为18.46%、19.81%和17.59%。土壤酸可提取态分布比例显著下降、残渣态分布比例显著上升,有效降低了土壤中Cd的生物有效性和迁移性。此外,T1~T5处理水稻植株根、茎、叶、稻米Cd含量较CK处理显著下降,降幅分别为18.28%~84.68%、26.92%~93.08%、21.79%~88.46%和27.76%~96.84%。各处理的稻米膳食风险指数及水稻植株潜在生态风险指数顺序为CK>T3>T1>T2>T5>T4。【结论】施加竹炭有机肥+叶面阻控剂处理降Cd效果更佳,更有利于保障研究区的稻谷安全,降低水稻茎叶还田利用和人体食用健康风险。

关键词：土壤 Cd污染稻谷风险评价竹炭有机肥叶面阻控剂

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Repeated measurement of growth-differentiation factor-15in Chinese Han patients with post-myocardial infarction chronic heart failure

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Journal of Geriatric Cardiology 2018年第10期15卷 618-627页

作者： Ji-Xuan liu Yan-Ping LI Bo-Han liu xiao-Jing ZHAO Ze-Yu ZHANG Jin-Da WANG Qian JIA chun-Lei liu xiao-Jian GAO Zhen-Guo XU Hua-Wei ZHANG Lin-Nan SONG Zhi-Jun SUN Kun-Lun HE Beijing Key Laboratory of Chronic Heart Failure Precision Medicine Chinese PLA General HospitalBeijingChina Transformation Medicine Centre Chinese PLA GeneraI HospitalBeijingChina Department of Cardiovascular Chinese PLA General HospitalBeijingChina Department of Geriatric Cardiology Chinese PLA General HospitalBeijingChina Outpatient Department of North China Military Materials Procurement Bureau TianjinChina Queen Mary College of Nanchang University NanchangChina

Background Growth-differentiation factor-15(GDF-15)is a promising prognostic biomarker in patients with chronic heart failure (CHF).Comparatively little is known about the value of repeated measurement of GDF-15with CI-IF in Chinese Han *** study sought to identify the clinical value of repeated measurement of GDF-15in Chinese Han patients with post-myocardial infarction CHF. Methods In total,232consecutive Chinese Hart patients with post-myocardial infarction CHF were enrolled prospectively from January 2014to June *** plasma concentration of GDF-15was determined on admission and over *** were followed up for all-cause death and a composite outcome of major adverse cardiac events (MACE)included all-cause death,myocardial infarction and first heart failure (HF)*** with other clinical variables and adverse outcomes of repeated measurement of GDF-15 was *** The median baseline GDF-15level was 2025ng/*** GDF-15was moderately associated with baseline N-terminal pro-B type nalriuretic peptide (NT-proBNP)(coefficient 0.561,P <0.001).During a median follow-up of 20months,there were 53deaths and ***-I5remained an independent predictor of all-cause death (adjusted hazard ratio 1.826per 1Ln U,95%CI: 1.037-8.360;P =0.037)and MACE (adjusted hazard ratio 2.243per I Ln U,95%CI:1.181-1.775;P <0.001)adjusted for established risk *** measurement of GDF-15was performed in 173survivals over *** of GDF-15over 12months was associ- ated with dilatation of left ventricle and acted as an independent predictor of subsequent all-canse death (adjusted HR =3.164,95%CI: 1.245~.041;P =0.015).In the joint model,GDF-15was also shown to be a risk factor for all-cause death (HR =2.749,95%CI: 1.667-3.831;P <0.001)and MACE (FIR =2.434,95%CI:1.425-3.443;P <0.001).Conclusions Repeated measurements of GDF-15 have promising prognostic value of the risk of all-cause death in Chinese Han patients

关键词： Chronic heart failure Growth-differentiation factor-15 Left ventricular remodeling Myocardial infarction Prognosis

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Characterization of TaCOMT genes associated with stem lignin content in common wheat and development of a gene-specific marker

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Journal of Integrative Agriculture 2019年第5期18卷 939-947页

作者： FU Lu-ping xiao Yong-gui YAN Jun liu Jin-dong WEN Wei-e ZHANG Yong XIA Xian-chun HE Zhong-hu National Wheat Improvement Center/Institute of Crop Sciences Chinese Academy of Agricultural Sciences Institute of Cotton Research Chinese Academy of Agricultural Sciences International Maize and Wheat Improvement Center (CIMMYT) China Office

Stem lignin content(SLC) in common wheat(Triticum aestivum L.) contributes to lodging resistance. Caffeic acid 3-O-methyltransferase(COMT) is a key enzyme involved in lignin biosynthesis. Characterization of TaCOMT genes and development of gene-specific markers could enable marker-assisted selection in wheat breeding. In the present study, the full-length genomic DNA(gDNA) sequences of TaCOMT genes located on chromosomes 3 A, 3 B, and 3 D were cloned by homologous cloning. Two allelic variants, TaCOMT-3 Ba and TaCOMT-3 Bb, were identified and differed by a 222-bp insertion/deletion(InDel) in the 3′-untranslated region(3′-UTR). A co-dominant gene-specific marker based on this InDel was developed and designated as Ta COMT-3 BM. A total of 157 wheat cultivars and advanced lines grown in four environments were used to validate the associations between allelic patterns and SLC. The SLC of cultivars with TaCOMT-3 Ba was significantly(P<0.01) higher than that of those with TaCOMT-3 Bb, and the marker TaCOMT-3 BM could be effectively used in wheat breeding.

关键词： Triticum aestivum L. caffeic acid 3-O-methyltransferase stem lignin content gene-specific marker lodging resistance

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Unveiling the mechanical response and accommodation mechanism of pre-rolled AZ31 magnesium alloy under high-speed impact loading

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Journal of Magnesium and Alloys 2022年第4期10卷 1096-1108页

作者： xiao liu Hui Yang Biwu Zhu Yuanzhi Wu Wenhui liu Changping Tang Key Laboratory of High Temperature Wear Resistant Materials Preparation Technology of Hunan Province Hunan University of Science and TechnologyXiangtanHunan 411201China Research Institute of Automobile Parts Technology Hunan Institute of TechnologyHengyangHunan 421002China

Split Hopkinson pressure bar(SHPB)tests were conducted on pre-rolled AZ31 magnesium alloy at 150–350℃ with strain rates of 2150s-1,3430s^(-1) and *** mechanical response,microstructural evolution and accommodation mechanism of the pre-rolled AZ31 magnesium alloy under high-speed impact loading were *** twin and shear band are prevailing at low temperature,and the coexistence of twins and recrystallized grains is the dominant microstructure at medium temperature,while at high temperature,dynamic recrystallization(DRX)is almost *** increment of temperature reduces the critical condition difference between twinning and DRX,and the recrystallized temperature decreases with increasing strain *** mechanical response is related to the competition among the shear band strengthen,the twin strengthen and the fine grain strengthen and determined by the prevailing grain *** fine grain strengthen could compensate soften caused by the temperature increase and the reduction of twin and shear *** high-speed deformation,different twin variants,introduced by pre-rolling,induce different deformation mechanism to accommodate plastic deformation and are in favor for non-basal *** low temperature,the high-speed deformation is achieved by twinning,dislocation slip and the following deformation shear band at different deformation *** high temperature,the high-speed deformation is realized by twinning and dislocation slip of early deformation stage,transition shear band of medium deformation stage and DRX of final deformation stage.

关键词： Mechanical response Pre-twinning Accommodation mechanism Pre-rolled AZ31 magnesium alloy High-speed impact loading

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地质封存二氧化碳微生物转化研究进展

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生物工程学报 2024年第9期40卷 2884-2898页

作者：冯骁邓泽郭红光公丽刘丁瑞冯玺阳太原理工大学安全与应急管理工程学院山西太原030024 中国石油勘探开发研究院北京100083 太原理工大学矿业工程学院山西太原030024 太原理工大学原位改性采矿教育部重点实验室山西太原030024

碳捕获、利用与封存(carbon capture,utilization and storage,CCUS)是实现我国“双碳”目标的重要技术手段,微生物活动是CO_(2)地质封存过程中不可忽视的一部分。部分微生物可将封存CO_(2)转化为甲烷或有机酸以实现资源利用,也可转化... 详细信息

碳捕获、利用与封存(carbon capture,utilization and storage,CCUS)是实现我国“双碳”目标的重要技术手段,微生物活动是CO_(2)地质封存过程中不可忽视的一部分。部分微生物可将封存CO_(2)转化为甲烷或有机酸以实现资源利用,也可转化为碳酸盐实现长久固定,这些微生物活动有助于实现CO_(2)的稳定封存与负碳排放。本文分析了深地封存CO_(2)可能涉及的微生物甲烷化、液化、矿化机理,综述了各转化途径的研究进展。CO_(2)微生物甲烷化和液化技术能够将封存CO_(2)转化为甲烷或高值有机物,实现资源再利用,二者既能单独使用,也可以耦联应用以扩大CO_(2)生物转化的应用范围。CO_(2)微生物矿化利用微生物诱导碳酸钙沉淀将CO_(2)转化为方解石,是一种极具潜力的固定CO_(2)和限制CO_(2)逃逸技术。目前CO_(2)微生物转化仍处于起步探索阶段,亟须从转化原理及影响因素、转化效率、经济性和环保性、工艺条件等方面开展深入研究,建立健全CO_(2)地下原位生物转化理论技术体系,并与CCUS结合建立“捕、输、驱、封、转、采”一体化技术体系,助力CCUS的高值应用和我国“双碳”目标的实现。

关键词： CO_(2)地质封存 CO_(2)微生物转化生物甲烷化生物液化生物矿化

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青藏高原土壤微生物生物量对放牧强度响应的Meta分析

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草地学报 2024年第6期32卷 1913-1922页

作者：安海涛孙彩彩董全民杨晓霞张春辉赵新全青海大学省部共建三江源生态与高原农牧业国家重点实验室/青海大学畜牧兽医科学院/青海省高寒草地适应性管理重点实验室青海西宁810016

家畜放牧是青藏高原高寒草地最主要的利用方式,对草地生态系统具有重要影响。然而青藏高原微生物生物量对放牧强度的综合响应模式仍不清楚。因此,本文采用Meta分析方法,对青藏高原高寒草地不同放牧强度表层土壤(0~10 cm)有机碳、全氮含... 详细信息

家畜放牧是青藏高原高寒草地最主要的利用方式,对草地生态系统具有重要影响。然而青藏高原微生物生物量对放牧强度的综合响应模式仍不清楚。因此,本文采用Meta分析方法,对青藏高原高寒草地不同放牧强度表层土壤(0~10 cm)有机碳、全氮含量及微生物生物量变化进行了定量分析。结果表明,重度放牧显著降低了土壤有机碳、全氮、微生物生物量碳和微生物生物量氮含量,降幅分别为24.5%,16.0%,29.3%和24.7%;且重度放牧降低了细菌、真菌和放线菌的生物量。此外,线性混合效应模型分析表明,土壤微生物生物量碳和土壤微生物生物量氮含量在重度放牧强度下与土壤有机碳和全氮含量呈正相关关系。综上,重度放牧对青藏高原高寒草地土壤养分和微生物生物量具有显著的负效应,为维持草地植物多样性和生态系统功能,应保持较低的放牧压力。

关键词：青藏高原放牧强度土壤微生物生物量 Meta分析

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基于世界卫生组织康复胜任力架构构建辅助技术专业本科课程体系

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中国康复理论与实践 2024年第5期30卷 591-597页

作者：唐乐天刘妤姝杨亚茹崔良波肖晓飞滨州医学院特殊教育与康复学院山东烟台市264003 华东师范大学体育与健康学院上海市200241

目的基于世界卫生组织《国际功能、残疾和健康分类》(ICF)和康复胜任力架构(RCF)构建辅助技术专业本科课程体系,以培养具有胜任力的康复辅助技术工作者。方法运用ICF和RCF,基于现代康复辅具服务需求,定义康复辅助技术人员的胜任力,结合... 详细信息

目的基于世界卫生组织《国际功能、残疾和健康分类》(ICF)和康复胜任力架构(RCF)构建辅助技术专业本科课程体系,以培养具有胜任力的康复辅助技术工作者。方法运用ICF和RCF,基于现代康复辅具服务需求,定义康复辅助技术人员的胜任力,结合国家职业技能标准,构建康复辅助技术专业本科课程体系。结果基于ICF和RCF,结合国家职业技能标准,提炼了准入水平的辅助技术工作者的胜任力,设定了辅助技术本科专业教育目标,构建了辅助技术专业本科课程内容体系,并开发了核心课程。结论基于ICF和RCF,可构建辅助技术专业本科课程体系,有助于提高康复辅助技术专业人才的数量和质量,增加功能障碍者获得辅助技术的机会。

关键词：辅助技术课程康复胜任力职业技能标准

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基于提升学生核心素养的高中物理习题教学

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物理教学探讨 2024年第9期42卷 83-86页

作者：雷庆张春国龚小梅四川外语学院重庆第二外国语学校重庆400065 重庆市南岸区教师进修学院重庆401336 重庆市长寿区渡舟街道中心小学校重庆401235

高中物理课程注重提高学生核心素养,从物理观念、科学思维、科学探究、科学态度与责任等方面展现物理学科的育人功能。高中物理教材精心编排了多样化的练习题目。在教学实践中,教师需优化教学策略,精心挑选那些能够促进物理观念的形成... 详细信息

高中物理课程注重提高学生核心素养,从物理观念、科学思维、科学探究、科学态度与责任等方面展现物理学科的育人功能。高中物理教材精心编排了多样化的练习题目。在教学实践中,教师需优化教学策略,精心挑选那些能够促进物理观念的形成、锻炼科学思维、加强科学探究能力以及培育积极科学态度的习题,以此全方位提升学生核心素养。

关键词：高中物理核心素养物理习题教学策略

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