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Responses of phospholipase D and lipoxygenase to mechanical wounding in postharvest cucumber fruits

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第6期11卷 443-450页

作者： Yu-ying ZHAO chun-lu qian Ji-cheng CHEN Yan PENG Lin-chun MAO Department of Food Science and Nutrition College of Biosystem Engineering and Food Seience Zhejiang University Hangzhou 310029 China College of Horticulture and Gardening Yangtze University Jingzhou 434025 China

This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus ***-2） ***-associated Ca2＋ content,activities and gene expression of PLD and LOX,and contents of phosphatidylcholine（PC）,phosphatidylinositol（PI）,and phosphatidic acid（PA） were determined in cucumber fruits following mechanical *** show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding,while membrane-associated Ca^2＋ content *** with the increase of PLD and LOX activities,accumulation of PA and losses of PC and PI were observed in all fruits,but there were differences of degrees between wounded and control *** suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding *** activation of PLD and LOX might be the result of gene expression,which could be This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus ***-2） ***-associated Ca^2＋ content,activities and gene expression of PLD and LOX,and contents of phosphatidylcholine（PC）,phosphatidylinositol（PI）,and phosphatidic acid（PA） were determined in cucumber fruits following mechanical *** show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding,while membrane-associated Ca2＋ content *** with the increase of PLD and LOX activities,accumulation of PA and losses of PC and PI were observed in all fruits,but there were differences of degrees between wounded and control *** suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding *** activation of PLD and LOX might be

关键词： Cucumber Mechanical wounding Phospholipase D Lipoxygenase Phospholipid

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Hydrogen production by mixed culture of several facultative bacteria and anaerobic bacteria

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Progress in Natural Science:Materials International 2011年第6期21卷 506-511页

作者： chun-xiang qian,lu-yuan CHEN,Hui RONG,Xiao-ming YUAN School of Materials Science and Engineering,Southeast University,Nanjing 211189,China School of Materials Science and Engineering Southeast University

The characteristic of hydrogen production by facultative anaerobic bacteria,obligate anaerobic bacteria and their mixed culture was studied by the batch culture *** results showed that,due to the synergistic effect between facultative bacteria and anaerobic bacteria,the ability of hydrogen production in the mixed culture was much better than that in the pure culture. Especially,the culture Scheme No.7 mixed up with three strains(Bacterium.E:Bacterium.B:Bacterium.P = 1:1:1) not only had the best hydrogen production capacity(1.885 mol H2/mol glucose) and maximum average hydrogen production rate(212.2 mL/(L-h)), but also had stable hydrogen production under continuous culture conditions,which was 1.968 mol H2/mol glucose.

关键词： hydrogen production bacteria producing hydrogen mixed culture

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Construction of a Metallacyclopentadiene Ring through the Attack of Carbanions to M≡C Bond Followed by C—H Activation

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Chinese Journal of Chemistry 2024年第3期42卷 235-242页

作者： chun Tang Shengjie Zhang Zhengyu lu qian Li Ming luo Dafa Chen Haiping Xia State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical EngineeringCollaborative Innovation Center of Chemistry for Energy MaterialsXiamen UniversityXiamenFujian361005 China Shenzhen Grubbs Institute and Guangdong Provincial Key Laboratory of Catalysis Department of ChemistrySouthern University of Science and TechnologyShenzhenGuangdong518055 China

Metallacyclopentadienes are important metallacycles and regarded as intermediates in many reactions,therefore,new methods to achieve them are *** this study,a formal[3+2]method,through the reactions of an osmapentalyne with benzyl carbanions,was *** reactions underwent a nucleophilic attack of carbanions to the Os≡C bond,followed by C—H activation to form the five-membered osmacyclopentadiene *** of the reactions were carried out at room temperature,the substituents on the aromatic rings of benzyl carbanions are diverse,and the resulting products contain an Os—H bond,representing a novel type of 10C-carbolong *** work provides a new convenient route to construct metallacyclopentadienes,which is expected to further promote the development of such a type of substances.

关键词： Metallacyclopentadiene Carbolong chemistry Osmium C–H activation Nucleophilic addition

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AGK2 pre-treatment protects against thioacetamide-induced acute liver failure via regulating the MFN2-PERK axis and ferroptosis signaling pathway

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Hepatobiliary & Pancreatic Diseases International 2024年第1期23卷 43-51页

作者： Qing-Qi Zhang qian Chen Pan Cao chun-Xia Shi lu-Yi Zhang lu-Wen Wang Zuo-Jiong Gong Department of Infectious Diseases Renmin Hospital of Wuhan UniversityWuhan 430060China

Background:Acute liver failure(ALF)is an unpredictable and life-threatening critical *** pathological characteristic of ALF is massive necrosis of hepatocytes and lots of inflammatory cells infiltration which may lead to multiple organ ***:Animals were divided into 3 groups,normal,thioacetamide(TAA,ALF model)and TAA+*** L02 cells were divided into 5 groups,normal,TAA,TAA+mitofusin 2(MFN2)-siRNA,TAA+AGK2,and TAA+AGK2+MFN2-siRNA *** liver histology was evaluated with hematoxylin and eosin staining,inositol-requiring enzyme 1(IRE1),activating transcription factor 6β(ATF6β),protein kinase R(PKR)-like endoplasmic reticulum kinase(PERK)and phosphorylated-PERK(p-PERK).C/EBP homologous protein(CHOP),reactive oxygen species(ROS),MFN2 and glutathione peroxidase 4(GPX4)were measured with Western blotting,and cell viability and liver chemistry were also *** endoplasmic reticulum membranes(MAMs)were measured by ***:The liver tissue in the ALF group had massive inflammatory cell infiltration and hepatocytes necrosis,which were reduced by AGK2 *** comparison to the normal group,apoptosis rate and levels of IRE1,ATF6β,p-PERK,CHOP,ROS and Fe2+in the TAA-induced ALF model group were significantly increased,which were decreased by AGK2 *** levels of MFN2 and GPX4 were decreased in TAA-induced mice compared with the normal group,which were enhanced by AGK2 *** with the TAA-induced L02 cell,apoptosis rate and levels of IRE1,ATF6β,p-PERK,CHOP,ROS and Fe2+were further increased and levels of MFN2 and GPX4 were decreased in the MFN2-siRNA ***2 pre-treatment decreased the apoptosis rate and levels of IRE1,ATF6β,p-PERK,CHOP,ROS and Fe2+and enhanced the protein expression of MFN2 and GPX4 in MFN2-siRNA treated L02 *** observation showed that level of MAMs was promoted in the AGK2 pre-treatment group when compared with the TAA-induced gr

关键词： SIRT2 inhibitor AGK2 Acute liver failure MFN2 Ferroptosis

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A high-performance lithium anode based on N-doped composite graphene

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Rare Metals 2024年第3期43卷 1030-1036页

作者： Can Jiao Hao-Bo Sun Li Zhang Shang-qian Zhao Guo-Yao Pang chun-Rong Zhao Shi-Gang lu General Research Institute for Nonferrous Metals Beijing100088China China Automotive Battery Research Institute Co.Ltd Beijing101407China

Lithium(Li)metal is the most promising electrode for next-gene ration rechargeable *** order to push the commercialization of the lithium metal batteries,a kind of nitrogen(N)-doped composite graphene(NCG)adopted as the Li plating host was prepared to regulate Li metal nucleation and suppress dendrite ***,a new kind of sandwich-type composite lithium metal(STCL)electrode was developed to improve its *** STCL electrode can be used as convenient as a piece of Li foil but no dendrite *** a symmetric battery,the STCL electrode cycled for more than 4500 h with the overpotential of less than 40 *** due to the creative design,the STCL promises the Li-S battery with a prolonged cycling lifespan.

关键词： Lithium metal anode Lithiophilic N-doped composite graphene Sandwich-type composite lithium metal electrode

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Mechanistic insights into stepwise activation of malachite for enhancing surface reactivity and flotation performance

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International Journal of Minerals,Metallurgy and Materials 2024年第10期31卷 2159-2172页

作者： Qicheng Feng Wanming lu Han Wang qian Zhang State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization Faculty of Land Resource EngineeringKunming University of Science and TechnologyKunming 650093China Yunnan Key Laboratory of Green Separation and Enrichment of Strategic Mineral Resources Kunming University of Science and TechnologyKunming 650093China

Malachite is a common copper oxide mineral that is often enriched using the sulfidization-xanthate flotation ***,the direct sulfidization method cannot yield copper concentrate ***,a new sulfidization flotation process was developed to promote the efficient recovery of *** this study,Cu^(2+) was used as an activator to interact with the sample surface and increase its reaction sites,thereby strengthening the mineral sulfidization process and *** to single copper ion activation,the flota-tion effect of malachite significantly increased after stepwise Cu^(2+) *** potential,X-ray photoelectron spectroscopy(XPS),time-of-flight secondary ion mass spectroscopy(ToF-SIMS),scanning electron microscopy and energy dispersive spectrometry(SEM-EDS),and atomic force microscopy(AFM)analysis results indicated that the adsorption of S species was significantly enhanced on the mineral surface due to the increase in active Cu sites after Cu^(2+) stepwise ***,the proportion of active Cu-S spe-cies also increased,further improving the reaction between the sample surface and subsequent ***-transform infrared spec-troscopy(FT-IR)and contact angle tests implied that the xanthate species were easily and stably adsorbed onto the mineral surface after Cu^(2+) stepwise activation,thereby improving the hydrophobicity of the mineral ***,the copper sites on the malachite sur-face after Cu^(2+) stepwise activation promote the reactivity of the mineral surface and enhance sulfidization flotation of malachite.

关键词： malachite copper ions stepwise activation flotation mechanism enhanced sulfidization

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Experimental distillation of tripartite quantum steering with an optimal local filtering operation

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Photonics Research 2024年第3期12卷 552-562页

作者： qian-XI ZHANG XIAO-XU FANG HE lu School of Physics State Key Laboratory of Crystal MaterialsShandong UniversityJinan 250100China Shenzhen Research Institute of Shandong University Shenzhen 518057China

Multipartite Einstein-Podolsky-Rosen(EPR)steering admits multipartite entanglement in the presence of uncharacterized verifiers,enabling practical applications in semi-device-independent *** applications generally require stronger steerability,while the unavoidable noise weakens steerability and consequently degrades the performance of quantum information ***,we propose the local filtering operation that can maximally distill genuine tripartite EPR steering from N copies of three-qubit generalized Greenberger-Horne-Zeilinger states,in the context of two semi-device-independent *** optimal filtering operation is determined by the maximization of assemblage *** and numerical results indicate the advantage of the proposed filtering operation when N is finite and the steerability of initial assemblages is ***,a proof-of-principle demonstration of two-copy distillation is realized with the optical *** advantage of the optimal local filtering operation is confirmed by the distilled assemblage in terms of higher assemblage fidelity with perfectly genuine tripartite steerable assemblages,as well as the greater violation of the inequality to witness genuine tripartite steerable *** results benefit the distillation of multipartite EPR steering in practice,where the number of copies of initial assemblages is generally finite.

关键词： filtering distillation optimal

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The joint knowledge reasoning model based on knowledge representation learning for aviation assembly domain

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Science China(Technological Sciences) 2024年第1期67卷 143-156页

作者： LIU PeiFeng qian lu lu Hu XUE Lei ZHAO XingWei TAO Bo State Key Laboratory of Digital Manufacturing Equipment and Technology Department of MechanicalHuazhong University of Science and TechnologyWuhan 430074China School of Transportation and Logistics Engineering Wuhan University of Technology(WHUT)Wuhan 430063China Shanghai Aircraft Manufacturing Co. Ltd.Shanghai 201324China

Knowledge graph technology is widely applied in the domain of general knowledge reasoning with an excellent *** fine-grained professional fields,professional knowledge graphs can provide more accurate information in practical industrial *** on an aviation assembly domain-specific knowledge graph,the article constructs a joint knowledge reasoning model,which combines a named entity recognition model and a subgraph embedding learning *** performing knowledge reasoning tasks,the two models vectorize entities,relationships and entity attributes in the same space,so as to share parameters and optimize learning *** knowledge reasoning model,which provides intelligent question answering services,is able to reduce the assembly error rate and improve the assembly *** system can accurately solve general knowledge reasoning problems in the assembly process in actual industrial scenarios of general assembly and component assembly under interference-free ***,this paper compares the proposed knowledge reasoning model based on knowledge representation learning and the question-answering system based on large-scale pre-trained *** the application scenario of system functional testing in general assembly,the joint model attains an accuracy rate of 95%,outperforming GPT with 78%accuracy and enhanced representation through knowledge integration with 71%accuracy.

关键词： intelligent manufacturing knowledge graph aviation assembly knowledge representation knowledge-based question an-swering

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Enhancing source domain availability through data and feature transfer learning for building power load forecasting

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Building Simulation 2024年第4期17卷 625-638页

作者： Fanyue qian Yingjun Ruan Huiming lu Hua Meng Tingting Xu College of Mechanical and Energy Engineering Tongji UniversityShanghai200092China United Automotive Electronic Systems Co. LtdShanghai201206China

During the initial phases of operation following the construction or renovation of existing buildings,the availability of historical power usage data is limited,which leads to lower accuracy in load forecasting and hinders normal ***,by transferring load data from similar buildings,it is possible to enhance forecasting ***,indiscriminately expanding all source domain data to the target domain is highly likely to result in negative transfer *** study explores the feasibility of utilizing similar buildings(source domains)in transfer learning by implementing and comparing two distinct forms of multi-source transfer ***,this study focuses on the Higashita area in Kitakyushu City,Japan,as the research *** buildings that exhibit the highest similarity to the target buildings within this area were selected for ***,the two-stage TrAdaBoost.R^(2) algorithm is used for multi-source transfer learning,and its transfer effect is ***,the application effects of instance-based(IBMTL)and feature-based(FBMTL)multi-source transfer learning are compared,which explained the effect of the source domain data on the forecasting accuracy in different transfer *** results show that combining the two-stage TrAdaBoost.R^(2) algorithm with multi-source data can reduce the CV-RMSE by 7.23%compared to a single-source domain,and the accuracy improvement is *** the same time,multi-source transfer learning,which is based on instance,can better supplement the integrity of the target domain data and has a higher forecasting ***,IBMTL tends to retain effective data associations and FBMTL shows higher forecasting *** findings of this study,which include the verification of real-life algorithm application and source domain availability,can serve as a theoretical reference for implementing transfer learning in load forecasting.

关键词： building power load multi-source transfer learning two-stage TrAdaBoost.R2 source domain availability

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CNN-LSTM based incremental attention mechanism enabled phase-space reconstruction for chaotic time series prediction

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Journal of Electronic Science and Technology 2024年第2期22卷 77-90页

作者： Xiao-qian lu Jun Tian qiang Liao Zheng-Wu Xu lu Gan Bidding and Purchasing Center University of Electronic Science and Technology of ChinaChengdu611731China Yibin Research Institute University of Electronic Science and Technology of ChinaYibin644000China School of Information and Communication Engineering University of Electronic Science and Technology of ChinaChengdu611731China

To improve the prediction accuracy of chaotic time series and reconstruct a more reasonable phase space structure of the prediction network,we propose a convolutional neural network-long short-term memory(CNN-LSTM)prediction model based on the incremental attention ***,a traversal search is conducted through the traversal layer for finite parameters in the phase ***,an incremental attention layer is utilized for parameter judgment based on the dimension weight criteria(DWC).The phase space parameters that best meet DWC are selected and fed into the input ***,the constructed CNN-LSTM network extracts spatio-temporal features and provides the final prediction *** model is verified using Logistic,Lorenz,and sunspot chaotic time series,and the performance is compared from the two dimensions of prediction accuracy and network phase space ***,the CNN-LSTM network based on incremental attention is compared with long short-term memory(LSTM),convolutional neural network(CNN),recurrent neural network(RNN),and support vector regression(SVR)for prediction *** experiment results indicate that the proposed composite network model possesses enhanced capability in extracting temporal features and achieves higher prediction ***,the algorithm to estimate the phase space parameter is compared with the traditional CAO,false nearest neighbor,and C-C,three typical methods for determining the chaotic phase space *** experiments reveal that the phase space parameter estimation algorithm based on the incremental attention mechanism is superior in prediction accuracy compared with the traditional phase space reconstruction method in five networks,including CNN-LSTM,LSTM,CNN,RNN,and SVR.

关键词： Chaotic time series Incremental attention mechanism Phase-space reconstruction

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