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MdWRKY15 improves resistance of apple to Botryosphaeria dothidea via the salicylic acid-mediated pathway by directly binding the MdICS1 promoter^FA

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Journal of Integrative Plant Biology 2020年第4期62卷 527-543页

作者： Xian-Yan Zhao Chen-hui Qi Han Jiang Ming-Shuang Zhong chun-Xiang You yuan-yuan Li Yu-Jin Hao State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple College of HorticultureNorthwest A&F UniversityYangling 712100China State Key Laboratory of Crop Biology MOA Key Laboratory of Horticultural Crop Biology and Germplasm InnovationShandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai-An 271018China

Isochorismate synthase(ICS) plays an essential role in the accumulation of salicylic acid(SA) and plant disease resistance. Diseases caused by Botryosphaeria dothidea affect apple yields. Thus, it is important to understand the role of ICS1 in disease resistance to B. dothidea in apple. In this study, SA treatment enhanced the resistance to B. dothidea. Md ICS1 was induced by B. dothidea and enhanced the resistance to B. dothidea. Md ICS1 promoter analysis indicated that the W-box was vital for the response to B. dothidea treatment. Md WRKY15 was found to interact with the W-box using yeast one-hybrid screening. Subsequently, the interaction was confirmed by EMSA, yeast one-hybrid, Ch IP-PCR, and quantitative PCR assays. Moreover, luciferase and GUS analysis further indicated thatMd ICS1 was transcriptionally activated by Md WRKY15. Finally, we found the function of Md WRKY15 in the resistance to B. dothidea was partially dependent on Md ICS1 from the phenotype of transgenic apples and calli. In summary, we revealed that Md WRKY15 activated the transcription of Md ICS1 by directly binding to its promoter to increase the accumulation of SA and the expression of disease-related genes, thereby resulting in the enhanced resistance to *** in the SA biosynthesis pathway.

关键词： resistance thereby fir

Investigation of formation of dimeric G-quadruplex of HIV-1 integrase inhibitor by nuclear magnetic resonance

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Chinese Chemical Letters 2008年第9期19卷 1108-1110页

作者： hui hui Li Gu yuan Beifing National Laboratory for Molecular Sciences Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education Department of Chemical Biology College of Chemistry and Molecular Engineering Peking University Beifing 100871 China

In this research, an unusually dimeric G-quadruplex of d（GGGTGGGTGGGTGGGT）（SI）, the potent nanomolar HIV-1 integrase inhibitor, was detected by nuclear magnetic resonance （NMR）. This result has been confirmed b... 详细信息

关键词： G-quadruplex HIV-1 integrase inhibitor Nuclear magnetic resonance

北京市市售牡蛎中诺如病毒核酸检测及定量分析

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中国食品卫生杂志 2017年第2期29卷 126-130页

作者：江涛韩春卉张宏元张靖王佳慧李楠吕涵阳李凤琴国家食品安全风险评估中心卫生部食品安全风险评估重点实验室北京100021

目的针对北京市市售牡蛎样品中诺如病毒污染水平及污染浓度进行定量分析研究。方法分离牡蛎消化腺,将消化腺匀浆处理,加入含有蛋白酶K的磷酸盐缓冲液,进行样品前处理,用试剂盒提取病毒RNA,用一步法实时荧光逆转录聚合酶链式反应(real ti... 详细信息

目的针对北京市市售牡蛎样品中诺如病毒污染水平及污染浓度进行定量分析研究。方法分离牡蛎消化腺,将消化腺匀浆处理,加入含有蛋白酶K的磷酸盐缓冲液,进行样品前处理,用试剂盒提取病毒RNA,用一步法实时荧光逆转录聚合酶链式反应(real time RT-PCR)检测诺如病毒RNA,并对阳性样品进行定量分析。结果共检测牡蛎样品356份,其中GGI阳性样品12份,GGII阳性样品39份,GGI和GGII同时为阳性的样品6份。对阳性样品中的诺如病毒核酸定量分析,核酸浓度在3.7×10~3~2.8×10~5基因拷贝/g(消化腺)之间。结论北京市市售牡蛎中存在诺如病毒污染的情况,需要加强对牡蛎中诺如病毒的污染监测,并开展污染水平风险评估,保障消费者食用安全,降低由诺如病毒引起的腹泻病的疾病负担。

关键词：诺如病毒牡蛎实时荧光逆转录聚合酶链式反应核酸检测食品安全食品污染物

Signaling Components Involved in Plant Responses to Phosphate Starvation

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Journal of Integrative Plant Biology 2008年第7期50卷 849-859页

作者： hui yuan Dong Liu Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Phosphorus is one of the macronutrients essential for plant growth and development. Many soils around the world are deficient in phosphate （Pi） which is the form of phosphorus that plants can absorb and utilize. To cope with the stress of Pi starvation, plants have evolved many elaborate strategies to enhance the acquisition and utilization of Pi from the environment. These strategies include morphological, biochemical and physiological responses which ultimately enable plants to better survive under low Pi conditions. Though these adaptive responses have been well described because of their ecological and agricultural importance, our studies on the molecular mechanisms underlying these responses are still in their infancy. In the last decade, significant progresses have been made towards the identification of the molecular components which are involved in the control of plant responses to Pi starvation. In this article, we first provide an overview of some major responses of plants to Pi starvation, then summarize what we have known so far about the signaling components involved in these responses, as well as the roles of sugar and phytohormones.

关键词： microRNA phosphate starvation phytohormones plant responses signal transduction pathways signaling components transcription factors.

Numerical Simulation of Bone Remodeling Coupling the Damage Repair Process in Human Proximal Femur

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Computer Modeling in Engineering & Sciences 2020年第11期125卷 829-847页

作者： Chuanyong Qu hui yuan Department of Mechanics School of Mechanical EngineeringTianjin UniversityTianjin300350China

Microdamage is produced in bone tissue under the long-termeffects of physiological loading,as well as age,disease and other *** remodeling can repair microdamage,otherwise this damage will undermine bone quality and even lead to *** this paper,the damage variable was introduced into the remodeling *** new remodeling algorithm contains a quadratic term that can simulate reduction in bone density after large numbers of loading *** model was applied in conjunction with the 3Dfinite elementmethod(FEM)to the remodeling of the proximal *** results showed that the initial accumulation of fatigue damage led to an increase in density but when the damage reached a certain level,the bone density decreased rapidly until the femur *** the accumulation of damage,bone remodeling was coupled with fatigue damage to maintain the function of *** the accumulation of damage reached a certain level,bone remodeling failed to repair the accumulated fatigue damage in time,and continued cyclic loading significantly weakened the loadbearing capacity of the *** new mathematical model not only predicts fatigue life,but also helps to further understand the compromise between damage repair and damage accumulation,which is of great significance for the prevention and treatment of clinical bone diseases.

关键词： Bone remodeling fatigue damage mathematical equation proximal femur FE analysis

Hydrogen production by mixed culture of several facultative bacteria and anaerobic bacteria

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Progress in Natural Science:Materials International 2011年第6期21卷 506-511页

作者： chun-xiang QIAN,Lu-yuan CHEN,hui RONG,Xiao-ming yuan School of Materials Science and Engineering,Southeast University,Nanjing 211189,China School of Materials Science and Engineering Southeast University Nanjing 211189 China

The characteristic of hydrogen production by facultative anaerobic bacteria,obligate anaerobic bacteria and their mixed culture was studied by the batch culture *** results showed that,due to the synergistic effect between facultative bacteria and anaerobic bacteria,the ability of hydrogen production in the mixed culture was much better than that in the pure culture. Especially,the culture Scheme No.7 mixed up with three strains(Bacterium.E:Bacterium.B:Bacterium.P = 1:1:1) not only had the best hydrogen production capacity(1.885 mol H2/mol glucose) and maximum average hydrogen production rate(212.2 mL/(L-h)), but also had stable hydrogen production under continuous culture conditions,which was 1.968 mol H2/mol glucose.

关键词： hydrogen production bacteria producing hydrogen mixed culture

Self-healing,Stretchable,Temperature-Sensitive and Strain-Sensitive Hydrogel-based Flexible Sensors

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Chinese Journal of Polymer Science 2023年第3期41卷 334-344,I0005页

作者： chun-Xia Zhao Min Guo Jie Mao Yun-Tao Li yuan-Peng Wu Hua Guo Dong Xiang hui Li School of Materials Science and Engineering Southwest Petroleum UniversityChengdu 610500China The Center of Functional Materials for Working Fluids of Oil and Gas Field Sichuan Engineering Technology Research Center of Basalt Fiber Composites Development and ApplicationSouthwest Petroleum UniversityChengdu 610500China State Key Lab of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengdu 610500China

Flexible hydrogels have shown promise as strain sensors in medical monitoring,human motion detection and intelligent *** a hydrogel strain sensor,certain challenges need to be urgently addressed for practical applications,such as the damage caused by external effects,leading to equipment failure,and the inability to perceive ambient temperature,resulting in single ***,a stretchable,self-healing and dual temperature-strain sensitive hydrogel,with a physically-crosslinked network,is designed by constructing multiple dynamic reversible *** oxide(GO)and iron ions(Fe^(3+))act as dynamic bridges in the cross-linked network and are mediated by the covalent and hydrogen bonding,rendering excellent stretchability to the *** reversible features of coordination interactions and hydrogen interactions endow excellent recoverability and self-healing ***,the incorporated N-isopropyl acrylamide(NIPAM)provides excellent temperature responsiveness to the hydrogel,facilitating the detection of external temperature ***,the hydrogels exhibited strain-sensitivity,with a wide working range of 1%-300%,fast response and electrical stability,which can be used as flexible sensors to monitor body motions,e.g.,speaking and the bending of finger,wrist,elbow and ***,the hydrogel possesses dual sensory capabilities,combining external temperature and strain,for potential applications in wearable multifunctional sensing devices.

关键词： Hydrogel sensor Self-healing Dual temperature-strain sensitive Monitor body motions

A note on parameterized Marcinkiewicz integrals with variable kernels

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Applied Mathematics(A Journal of Chinese Universities) 2009年第3期24卷 315-320页

作者： WANG hui ZHANG chun-jie Department of Mathematics Zhejiang University Hangzhou 310027 China Department of Mathematics Hangzhou Dianzi University Hangzhou 310018 China

In this paper,the parameterized Marcinkiewicz integrals with variable kernels defined by μΩ^ρ（f）（x）=（∫0^∞│∫│1-y│≤t Ω（x,x-y）/│x-y│^n-p f（y）dy│^2dt/t1＋2p）^1/2 are *** is proved that if Ω∈ L∞（R^n） × L^r（S^n-1）（r〉（n-n1p＇/n） is an odd function in the second variable y,then the operator μΩ^ρ is bounded from L^p（R^n） to L^p（R^n） for 1 〈 p ≤ max{（n＋1）/2,2}.It is also proved that,if Ω satisfies the L^1-Dini condition,then μΩ^ρ is of type（p,p） for 1 〈 p ≤ 2,of the weak type（1,1） and bounded from H1 to L1.

关键词： parameterized Marcinkiewicz integral variable kernel rotation method

Function spaces of Besov-type and Triebel-Lizorkin-type——a survey

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Applied Mathematics(A Journal of Chinese Universities) 2013年第4期28卷 405-426页

作者： YANG Da-chun yuan Wen School of Mathematical Sciences Beijing Normal University Laboratory of Mathematics and Complex Systems Ministry of Education Institut fr Analysis Karlsruhe Institute of TechnologyD-76128 Karlsruhe Germany

This article is devoted to presenting a recapitulative introduction for the theory of Besov-type and Triebel-Lizorkin-type spaces developed in recent years.

关键词： Besov space Triebel-Lizorkin space embedding atom molecule wavelet local mean Peetre maximal function dual complex interpolation