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Short‑chain fatty acids(SCFAs)as potential resuscitation factors that promote the isolation and culture of uncultured bacteria in marine sediments

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Marine Life Science & Technology 2023年第3期5卷 400-414页

作者： chun-shui sun Liu-Yan Zhou Qi-Yun Liang Xiao-Man Wang Yi-Xuan Lei Zhen-Xing Xu Feng-Qing Wang Guan-Jun Chen Zong-Jun Du Da-Shuai Mu Marine College Shandong UniversityWeihai 264209China State Key Laboratory of Microbial Technology Institute of Microbial TechnologyShandong UniversityQingdao 266237China Weihai Research Institute of Industrial Technology of Shandong University Weihai 264209China Institute of Microbiology Applications Xinjiang Academy of Agricultural SciencesUrumqi 830000China Tancheng County Inspection and Testing Center Tancheng 276100China Department of Applied Biological Chemistry Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-KuTokyo 113-8657Japan Max Planck Institute for Marine Microbiology Celsiusstraße 128359 BremenGermany

Many marine bacteria are difcult to culture because they are dormant,rare or found in *** culturing has been widely tested as an important strategy to isolate rare or dormant ***,many more mechanisms remain ***,based on 16S rRNA gene high-throughput sequencing and metabolomics technology,it was found that the short-chain fatty acids(SCFAs)in metabolites were signifcantly correlated with uncultured bacterial groups during enrichment cultures.A pure culture analysis showed that the addition of SCFAs to media also resulted in high efciency for the isolation of uncultured strains from marine *** a result,238 strains belonging to 10 phyla,26 families and 82 species were successfully *** uncultured rare taxa within Chlorobi and Kiritimatiellaeota were successfully *** the newly isolated uncultured microbes,most genomes,***,possess SCFA oxidative degradation genes,and these features might aid these microbes in better adapting to the culture media.A further resuscitation analysis of a viable but non-culturable(VBNC)Marinilabiliales strain verifed that the addition of SCFAs could break the dormancy of Marinilabiliales in 5 days,and the growth curve test showed that the SCFAs could shorten the lag phase and increase the growth ***,this study provides new insights into SCFAs,which were frst studied as resuscitation factors in uncultured marine ***,this study can help improve the utilisation and excavation of marine microbial resources,especially for the most-wanted or key players.

关键词： Marine bacteria SCFAs VBNC Resuscitation culture

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Surface embedded binary Co-Cu hydroxy phosphate over surface of oxy/hydroxides for efficient nitrate reduction to ammonia

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Nano Research 2025年第1期18卷 195-207页

作者： chun Zhu Yifei Yang Yike Jing Zhen Zeng Xuesong Yi Wei sun Fei Yang Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province School of Environmental Science and EngineeringHainan UniversityHaikou 570228China

Electrochemical nitrate reduction reaction(NO3RR)to ammonia is a promising approach to address excess Ncontamination in aqueous environment but suffers from a limited ammonia yield and selectivity due to the low concentration and sluggish *** work reports a three-dimensional porous electrode with binary Co_(2)Cu_(1)hydroxy phosphate embedded into the surface of Ni_(3)Co_(1)oxy/hydroxides(CoCu-PH@NiCo/Ni,“PH”means phosphate-hydroxide),which delivers a yield of 4.13 mg·h^(-1)·cm^(-2)with a high Faraday efficiency of 92.6%under a low nitrate concentration of 40 mM and provide an impressive *** embedded structure of the as-prepared catalyst exhibits unique metal coordination ligands and valence states different from the unitary *** electrochemical in situ infrared spectra and theoretical calculations revealed that the Co sites with the ability to dissociate water molecular to form adsorbed OH*can effectively provide the required protons for the deoxygenation and hydrogenation steps during NO3-conversion,thereby,effectively reducing the energy barrier of the key transition states in the NO3RR process to improve the intrinsic *** demonstrate that the rational design of binary components with the appropriate ability to activate water molecules as well as the threedimensional porous skeletal structure are expected to promote effectively the low-concentration nitrates conversion to ammonia.

关键词： nitrate reduction hydroxy phosphate surface embedded water dissociation active hydroxy adsorbed

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内质网应激与常见退行性骨骼疾病的发生与发展

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中国组织工程研究 2025年第6期29卷 1285-1295页

作者：钱琨李子卿孙水山东第一医科大学附属省立医院骨关节科山东省济南市250021 医学科技创新中心骨科实验室山东第一医科大学(山东省医学科学院)山东省济南市250117

背景:常见的退行性骨骼疾病,如骨关节炎、骨质疏松症和椎间盘退变,其具体的发病分子机制目前尚未明确,可能涉及内质网应激。目前,关于内质网应激在这些常见骨骼疾病发病机制中的系统作用和相关治疗进展的研究较为有限。目的:综述内质网... 详细信息

背景:常见的退行性骨骼疾病,如骨关节炎、骨质疏松症和椎间盘退变,其具体的发病分子机制目前尚未明确,可能涉及内质网应激。目前,关于内质网应激在这些常见骨骼疾病发病机制中的系统作用和相关治疗进展的研究较为有限。目的:综述内质网应激在常见的退行性骨骼疾病中的作用,深入探讨这些疾病的分子机制并提供新的防治思路和视角。方法:检索2000-2024年相关文献,以“内质网应激,骨骼疾病,未折叠蛋白反应,骨关节炎,骨质疏松症,椎间盘退变,自噬,凋亡,铁死亡,焦亡”为中文检索词检索中国知网、万方、维普数据库;以“endoplasmic reticulum stress,bone disease,unfolded protein response,osteoarthritis,osteoporosis,intervertebral disc degeneration,autophagy,apoptosis,ferroptosis,pyroptosis”为英文检索词检索PubMed、Web of Science数据库。排除重复和较陈旧的文献,共115篇文献符合纳入标准。结果与结论:①内质网应激在细胞生理调节中具有双重效应。轻度的内质网应激有助于促进成骨分化和细胞外基质合成,然而持续过度的内质网应激则会导致细胞死亡。②内质网应激诱导的细胞自噬、凋亡等与骨关节炎、骨质疏松症、椎间盘退变密切相关。③衰老、药物不良反应、代谢紊乱、钙平衡失调、不良生活习惯等多种原因可导致内质网应激的长期激活,从而引起骨重塑紊乱、软骨损伤、髓核细胞死亡等病理表现,最终导致骨关节炎、骨质疏松和椎间盘退变的发生。④对引发内质网应激的相关机制进行干预,有望在预防和治疗骨关节炎、骨质疏松症和椎间盘退变等常见退行性骨病方面发挥作用。

关键词：内质网应激骨骼疾病未折叠蛋白反应骨关节炎骨质疏松症椎间盘退变

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Is the Paris rulebook sufficient for effective implementation of Paris Agreement?

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Advances in Climate Change Research 2022年第4期13卷 600-611页

作者： Ruo-shui sun Xiang GAO Liang-chun DENG Can WANG School of Environment Tsinghua UniversityBeijing 100084China National Center for Climate Change Strategy and International Cooperation Beijing 100035China Center for Environmental Progress Wuhan 430070China

Glasgow Climate Conference concluded the negotiation on implementing rules for Paris Agreement and adopted the Glasgow Climate *** on practices under the UNFCCC and its Kyoto Protocol,Paris Agreement enhanced global climate change *** Agreement established a system centered around‘nationally determined contributions’(NDCs)of Parties,with a transparency framework to ensure information flow running the system,meanwhile facilitating implementation by Parties through the mechanism on facilitation and compliance,as well as urging Parties to enhance ambition both at individual level and aggregate level through the mechanism on Global *** doing so,it is logical that the system would lead to the achievement of the goal set by Paris ***,the system stil faces significant deficiency,such as lack of information timeliness,intransparency of some necessary information,hypothesis-based but not solution-based decision-making,and lack of effective assurance for developing countries to get finance and technology *** is concluded that this system is beneficial to long-term climate change policy decision and tracking progress of actions,while for shor-term decision-making,more comprehensive consideration is needed than only based on the mechanisms of and outcomes from the *** society should pay more attention to the progress of implementation,as well as ambition of various support provided to developing countries,than only the ambition of mitigation target numbers.

关键词： Global climate govermnance Paris Agreement Paris rulebook Nationally determined contribution Multilateral environmental agreement

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Persistent Variations in the East Asian Trough from March to April and the Possible Mechanism

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Advances in Atmospheric Sciences 2024年第4期41卷 737-753,I0002-I0004页

作者： shui YU Jianqi sun Nansen-Zhu International Research Center Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China

The East Asian trough(EAT)profoundly influences the East Asian spring *** this study,the relationship of the EATs among the three spring months is *** analysis shows that the variation in March EAT is closely related to that of April *** empirical orthogonal function(EEOF)analysis also confirms the co-variation of the March and April *** positive/negative EEOF1 features the persistent strengthened/weakened EAT from March to *** investigation indicates that the variations in EEOF1 are related to a dipole sea surface temperature(SST)pattern over the North Atlantic and the SST anomaly over the tropical Indian *** dipole SST pattern over the North Atlantic,with one center east of Newfoundland Island and another east of Bermuda,could trigger a Rossby wave train to influence the EAT in March−*** SST anomaly over the tropical Indian Ocean can change the Walker circulation and influence the atmospheric circulation over the tropical western Pacific,subsequently impacting the southern part of the EAT in March−*** the SST factors,the Northeast Asian snow cover could change the regional thermal conditions and lead to persistent EAT anomalies from March to *** three impact factors are generally independent of each other,jointly explaining large variations in the EAT ***,the signals of the three factors could be traced back to February,consequently providing a potential prediction source for the EAT variation in March and April.

关键词： spring East Asian trough sea surface temperature snow cover

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Precise Asymptotics for Lévy Processes

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Acta Mathematica Sinica,English Series 2007年第7期23卷 1265-1270页

作者： Zhi shui HU chun SU Department of Statistics and Finance University of Science and Technology of China

Let {X（t）, t ≥ 0} be a Lévy process with EX（1） = 0 and EX^2（1）〈 ∞. In this paper, we shall give two precise asymptotic theorems for {X（t）, t 〉 0}. By the way, we prove the corresponding conclusions for s... 详细信息

关键词： precise asymptotic Lévy process stable process Fuk-Nagaev type inequality

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Precision spectroscopy of atomic helium

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National Science Review 2020年第12期7卷 1818-1827页

作者： Yu R.sun shui-Ming Hu Hefei National Laboratory for Physical Sciences at Microscale iChem CenterUniversity of Science and Technology of China CAS Center for Excellence in Quantum Information and Quantum Physics University of Science and Technology of China

Helium is a prototype three-body system and has long been a model system for developing quantum mechanics theory and computational *** fine-structure splitting in the 2;P state of helium is considered to be the most suitable for determining the fine-structure constant α in *** more than 50 years of efforts by many theorists and experimentalists,we are now working toward a determination ofα with an accuracy of a few parts per billion,which can be compared to the results obtained by entirely different methods to verify the self-consistency of quantum ***,the precision spectroscopy of helium allows determination of the nuclear charge radius,and it is expected to help resolve the ’proton radius puzzle’.In this review,we introduce the latest developments in the precision spectroscopy of the helium atom,especially the discrepancies among theoretical and experimental results,and give an outlook on future progress.

关键词： precision spectroscopy helium quantum electrodynamics fine-structure constant nuclear charge radius

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Uncertain optimal model and solving method to platform scheduling problem in battlefield

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Journal of Systems Engineering and Electronics 2016年第1期27卷 157-165页

作者： Yu sun Peiyang Yao Dongdong shui Jieyong Zhang Information and Navigation College Air Force Engineering UniversityXi'an 710077China Army Police Force Engineering University Xi'an 710077China

The platform scheduling problem in battlefield is one of the important problems in military operational *** needs to minimize mission completing time and meanwhile maximize the mission completing accuracy with a limited number of *** the traditional certain models obtain some good results,uncertain model is still needed to be introduced since the battlefield environment is complex and *** uncertain model is prposed for the platform scheduling *** parameters in this model are set to be fuzzy or *** to the inherent disadvantage of the solving methods for traditional models,a new method is proposed to solve the uncertain ***,the practicability and availability of the proposed method are demonstrated with a case of joint campaign.

关键词： operational research platform scheduling uncertain model solving method.

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The Impact of Nonlinear Stochastic Perturbation on Dynamical Behavior of an HIV Infection System

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Journal of Systems Science & Complexity 2023年第2期36卷 632-655页

作者： sun Guanzhen LU chun Department of Environmental Science School of Environment and Municipal EngineeringQingdao University of TechnologyQingdao 266520China Department of Mathematics Qingdao University of TechnologyQingdao266520China

In this paper,the authors develop and study an HIV infection system with two distinct cell subsets and nonlinear stochastic ***,the authors obtain that the solution of the system is positive and ***,for the corresponding linear case,the authors derive a critical condition R0S similar to deterministic *** R0S>1,the authors establish sufficient conditions for the existence and uniqueness of an ergodic stationary distribution to the stochastic system,***,the authors give sufficient criterions for extinction of the *** proposed work provides a new method in overcoming difficulty conduced by nonlinear stochastic perturbation.

关键词： HIV model nonlinear stochastic perturbation stationary distribution two distinct cell subsets

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An encapsulated optical microsphere sensor for ultrasound detection and photoacoustic imaging

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Science China(Physics,Mechanics & Astronomy) 2022年第2期65卷 44-49页

作者： Jialve sun Jia-Wei Meng shui-Jing Tang Changhui Li Department of Biomedical Engineering College of EngineeringPeking UniversityBeijing 100871China ~State Key Laboratory for Mesoscopic Physics and Collaborative Innovation Center of Quantum Matter School of PhysicsPeking UniversityBeijing 100871China College of Future Technology Peking UniversityBeijing 100871

Optical whispering-gallery microresonators have attracted considerable interest for ultrasensitive ultrasound detection and photoacoustic imaging because of the combination of high quality factors and small cavity *** the last decade,ultrasonic sensors with on-chip microcavities have been extensively developed;however,they are unsuitable for the near-field photoacoustic microscopy of micro/nanoscale objects in complex biological environments and endoscopic *** this work,we developed ultrasonic sensors using two types of encapsulated microsphere resonators with different cavity materials.A noise equivalent pressure of as low as 160 Pa at 20 MHz was achieved with the acoustic response up to 70 MHz at-6 d ***,the microsensor was used for photoacoustic microscopy in which we successfully performed 3 D imaging of hairs and leaf *** microsphere ultrasonic sensor has considerable potential as a probe-type ultrasonic detector for near-field photoacoustic microscopy of micro/nanoscale objects such as subcellular structures and high-resolution endoscopic photoacoustic imaging with its high sensitivity and wide bandwidth.

关键词： optical whispering gallery micro-resonators ultrasound sensing photoacoustic imaging

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