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中国神经科学学会第十届全国学术会议

作者： chun-ping chu De-Lai Qiu Cellular Function Research Center Yanbian University Department of Physiology and Pathophysiology College of MedicineYanbian University

The cerebellum receives a wide variety of sensory inputs and generates motor-related outputs according to internal rules of *** climbing fiber(CF) and mossy fiber(MF) are condidered to transfer sensory information into cerebellar cortex,the CFs activate the Purkinje cells(PCs) directly by discharging complex spikes,whereas the mossy fibers excite them indirectly via the granule cells by discharging simple *** sensory stimulus precisely evokes responses in cerebellar PCs,the cellular mechanisms of sensory information processing in cerebellar cortex are ***,we studied the synaptic responses of cerebellar molecular layer interneurons(MLIs) and PCs evoked by air-puff stimulation of the ipsilateral whisker pad by in vivo whole-cell patch-clamp recording techniques in urethane-anesthetized ICR *** found that air-puff stimulus failed to provoke either simple or complex spikes in PCs,but evoked inhibitory postsynaptic potentials(IPSPs) in the somas of *** voltage-clamp,the stimulus evoked a sequence of transient inward currents followed by strong outward currents in the somas and dendrites in *** outward currents,revealed excitatory postsynaptic currents(EPSCs) in somas and a temporal summation of parallel fiber EPSCs in PC ***,the stimulus evoked spike firing and rapid EPSCs in MLIs,and the highest amplitude,shortest duration EPSCs were recorded from MLIs deep in the molecular layer,which expressed basket-type synaptic *** MLI to PC responses,time to peak of PC IPSP was significantly slower than MLI recorded *** GABA receptors uncovered larger EPSCs in PCs whose time to peak,half-width and 10-90%rising time were also significantly slower than in *** labeling indicated that the MLIs(but not PCs) are *** findings indicated that tactile face stimulation evoked rapid excitation in MLIs and inhibition of PCs in mouse cerebellar cortex via the MF-granule

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Mechanisms of spontaneous climbing fiber discharge-evoked pauses and output modulation of cerebellar Purkinje cell in mice

Mechanisms of spontaneous climbing fiber discharge-evoked pa...

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中国生理学会张锡钧基金会第十四届全国青年优秀生理学学术论文交流及评奖会议、中国生理学会第十二届全国青年生理学工作者学术会议

作者：张馨元 Xian-Hua Jin chun-ping chu De-Lai Qiu Key Laboratory of Cellular Function and Pharmacology of Jilin Province Yanbian University Department of Physiology and Pathophysiology College of MedicineYanbian University Department of Neurology Affiliated Hospital of Yanbian University

Climbing fiber(CF)afferents modulate the frequency and patterns of cerebellar Purkinje cell(PC)simple spike(SS)activity,but its mechanism is *** the present study,we investigated the mechanisms of spontaneous climbing fiber discharge-evoked pauses and the output modulation of cerebellar PCs in urethane-anesthetized mice using in vivo whole-cell recording techniques and

关键词： Mechanisms of spontaneous climbing fiber discharge-evoked pauses and output modulation of cerebellar Purkinje cell in mice

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Mechanisms of facial stimulation evoked long-term synaptic plasticity in the cerebellar granule cell layer in vivo in mice

Mechanisms of facial stimulation evoked long-term synaptic p...

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中国神经科学学会第十二届全国学术会议

作者： Chang Ma Ke-Min Zhang chun-ping chu De-Lai Qiu Yan-Hua Bing Key Laboratory of Cellular Function and Pharmacology of Jilin Province Yan Bian University

Objective To study the properties and possible mechanisms of the long-term synaptic plasticity induced by the facial tactile stimulation at mossy fiber-granule cell（MF-GC） synapses in urethane-anesthetized mice, by field potential recording technique and pharmacological methods. Methods Adult ICR mice（6–8-week-old） were anesthetized with urethane, and tracheotomized to avoid respiratory obstruction. The craniotomy was drilled to expose the cerebellar surface corresponding to Crus II. Results Our results showed that 4 Hz facial stimulation evoked long-term depression（LTD） of MF-GC synaptic transmission. However, 20 Hz facial stimulation evoked long-term potentiation（LTP） at MF-GC synapses. The MF-GC LTP was prevented by N-methyl-Daspartic acid receptor（NMDA） receptor antagonist, and could be pharmacological induced by application of NMDA. Notably, blockade of protein kinase C（PKC） pathway, 20 Hz facial stimulation failed to induce the MF-GC LTP. In addition, the MF-GC LTP could be enhanced by blocking γ-aminobutyrate A（GABAA） and cannabinoids I（CB1） receptors activities, but was decreased by inhibiting of nitric oxide synthesis. These results indicated that high frequency facial stimulation induced an NMDA receptor dependent, GABAA receptor and NO involved, and PKC signaling required LTP of MFGC synaptic transmission in vivo in mice. Conclusion Our results suggest that the sensory stimulation evoked MF-GC synaptic plasticity might be involved in motor learning in living animals.

关键词： Cerebellar cortex Tactile stimulation Long-term synaptic plasticity Granule cell layer In vivo

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An NMDA receptor and NO cascades dependent presynaptic LTP at glutamatergic synapses of the PVN magnocellular neurosecretory cells in vitro in rats

An NMDA receptor and NO cascades dependent presynaptic LTP a...

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2019中国生理学会学术年会暨张锡钧基金第十五届全国青年优秀生理学学术论文交流会及第十三届全国青年生理学工作者学术会议

作者： Bing-Xue LI Bin-Bin ZHANG Hua JIN De-Lai QIU chun-ping chu Brain Science Research Center Yanbian University Yanbian University Hospital Yanbian University

Objective The magnocellular neurosecretory cells(MNCs) of the hypothalamic paraventricular nucleus(PVN) integrate incoming signals to secrete oxytocin(OT) and vasopressin(VP) from their nerve terminals in the posterior pituitary *** has been demonstrated that high frequency stimulation(HFS) of afferent fibers induced an NMDA receptor-dependent LTP of excitatory glutamatergic inputs in the supraoptic nucleus(SON) MNCs of rat hypothalamic slices,therefore, the N-methyl-D-aspartic acid(NMDA) receptors-dependent plasticity in PVN MNCs may be involved in nitric oxide(NO) production and PKA signaling ***, the NO and PKA signaling cascades in the long-term plasticity in PVN MNCs is currently *** we used whole-cell patch-clamp recording, single-cell reverse transcription-multiplex polymerase chain reaction(SC-RT-mPCR), biocytin histochemistry and pharmacological methods to examine the mechanism of high frequency stimulus(HFS, 100 Hz)-induced long-term potentiation(LTP) at glutamatergic synapses in the PVN MNCs of juvenile male *** HFS-induced LTP at glutamatergic synapses was accompanied by a decrease in the paired-pulse ratio(PPR) of the PVN *** these MNCs, HFS-induced LTP persisted in the presence of a group 1 metabotropic glutamate receptor(mGluR1) antagonist;however, it was abolished by an NMDA receptor ***, HFS-induced LTP in the PVN MNCs was completely prevented by a nitric oxide synthase(NOS) *** application of an NO donor not only induced the LTP of excitatory glutamatergic inputs in the PVN MNCs, but also occluded the HFS-induced LTP in these ***, HFS-induced LTP in the PVN MNCs was also abolished by a specific protein kinase A(PKA) inhibitor, *** Our results indicate that a HFS can induce an NMDA receptor and NO cascades dependent on presynaptic glutamatergic LTP in the PVN MNCs via a PKA signaling pathway.

关键词： hypothalamic paraventricular nucleus(PVN) long-term synaptic plasticity NMDA receptor nitric oxide whole-cell recording protein kinase A

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Roles of N-methyl-D-aspartate receptors during the sensory stimulation-evoked field potential responses in mouse cerebellar cortical molecular layer

Roles of N-methyl-D-aspartate receptors during the sensory s...

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中国生理学会张锡钧基金会第十四届全国青年优秀生理学学术论文交流及评奖会议、中国生理学会第十二届全国青年生理学工作者学术会议

作者：玄芳玲 Guang-Jian Zhang Yin-Hua Xu chun-ping chu De-Lai Qiu Key Laboratory of Cellular Function and Pharmacology of Jilin Province Yanbian University Department of Physiology and Pathophysiology College of MedicineYanbian University

The functions of N-methyl-D-aspartate receptors(NMDARs)in cerebellar cortex have been widely studied under in vitro condition,but their roles during the sensory stimulation-evoked responses in the cerebellar cortical molecular layer in living animals are currently *** here investigated the roles of NMDARs during the air-puff stimulation on ipsilateral whisker pad-evoked field potential

关键词： Roles of N-methyl-D-aspartate receptors during the sensory stimulation-evoked field potential responses in mouse cerebellar cortical molecular layer

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Role of NMDARs during the sensory stimulation-evoked field potential responses in mouse cerebellar cortical molecular layer

Role of NMDARs during the sensory stimulation-evoked field p...

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中国神经科学学会第十二届全国学术会议

作者： Fang-Ling Xuan Ke-Ming Zhang Bing-Xue Li Li-Na Cui De-Lai Qiu chun-ping chu Cellular Function Research Center Yanbian University

Objective In mammalian cerebellar cortex, NMDARs are found existed on the membrane of granule cells（GC）, terminals of parallel fibers（PF）, molecular layer interneurons（MLI） and climbing fiber-Purkinje cell（PC） synapses, but the functional NMDARs are absence in the membrane of the adult PCs. We previously found that the air-puff stimulation on ipsilateral whisker pad evoked a sequence of transient tiny excitation response followed by strong inhibitory response in cerebellar molecular layer via mossy fiber pathway. In the present study, we investigated the role of NMDARs during the air-puff stimulation evoked field potential responses in cerebellar molecular layer of urethane-anesthetized mice. Methods Local field potential recordings from the cerebellar molecular layer were performed with an Axopatch-200 B amplifier. Sensory stimulation was performed using air-puff（60 ms, 60 psi） of ipsilateral whisker pad through a 12-gauge stainless tube connected with a pressurized injection system. The airpuff stimulations were controlled by a personal computer, which were synchronized with the electrophysiological recordings and delivered at 0.05 Hz through a Master 8 controller. All chemicals were applied to the cerebellar surface at 0.4 ml/min in ACSF. Results（1） Administration of NMDA affected the dynamic properties of sensory stimulation-evoked P1 in mouse cerebellar molecular layer, which expressed decreases in decay time, half-width and area under curve（AUC） of P1.（2） Application of NMDA induced significant increases in the decay time, half-width and AUC values of the facial stimulation-evoked excitatory responses（N1） in cerebellar molecular layer. However, NMDA did not significantly affect the amplitude, half-amplitude of N1 and rise time of N1.（3） Application an NMDAR blocker, D-APV（250 μM） abolished the facial stimulation-evoked GABAergic inhibitory components（P1） in mouse cerebellar molecular layer.（4） Blockade of NMDARs did not significantly change the amplitude and h

关键词： Cerebellar cortical molecular layer field potential recording NMDA receptors air-puff stimulation GABAergic responses parallel fiber

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Urocortin Ⅲ augments the GIRK channels activity in rat hypothalamic paraventricular nucleus magnocellular neurons in vitro

Urocortin Ⅲ augments the GIRK channels activity in rat hypo...

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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： chun-ping chu~(1,3),De-Lai QIU~(2,3*) 1 Function Experiment Center 2 Departments of Physiology and Pathophysiology,College of Medicine 3 Cellular Function Research Center,Yanbian University,Yanji,133000,China

Objective:We have previously reported that central administration of urocortinⅢelicited transient increase in mean arterial blood pressure and heart *** we aimed to examine the effects of urocortinⅢon the hypothalamic paraventricular nucleus(PVN) magnocellular neurons by whole-cell patch-clamp recordings and single-cell reverse transcription-polymerase chain reaction(RT-PCR) techniques,to explore the mechanisms of cardiovascular function modulation by urocortinⅢ.Methods:Hypothalamic slices were prepared from P12-14 day old male Wistar *** potentials and/or currents were monitored with an Axopatch-ID amplifier,acquired through a Digidata 1200 series analog-to-digital interface on a personal computer using Clampex 8.1 *** of cytoplasm and reverse transcription were carried out after the end of whole-cell *** and nested PCR amplification was used for screening oxytocin(OT),glyceraldehyde-3-phosphate dehydrogenase(GAPDH),CRFR-1 mRNA and CRFR-2 ***:Under current-clamp,urocortin HI(10 nmol/L to 600 nmol/L) resulted in a decrease in basal firing rate,and a dose-dependent hyperpolarization which was unaffected by the mixture of tetrodotoxin (0.5μmol/L),CNQX(10μmol/L) and bicuculline(10μmol/L).Under voltage-clamp,SCP(300 nmol/L) increased an inwardly rectifying K *** UrocortinⅢ-induced current was abolished by extracellular application of tertiapin(30 nmol/L),a selective blocker of G protein-activated,inward rectifying potassium(GIRK) channels. Moreover,the UrocortinⅢ-induced current was completely blocked by extracellular application of a selective corti-cotrophin -releasing factor(CRF) receptor 2 antagonist,antisauvagine-30(1μmol/L).Conclusion:These results suggest that UrocortinⅢinhibites the excitability of the hypothalamic paraventricular nucleus magnocellular neurons through enhancement of GIRK channels activity via CRF receptor 2 in vitro in mice.

关键词： urocortinⅢ PVN magnocellular neurons whole-cell patch-clamp recording single-cell RT-PCR

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Homozygous phytosterolemia and a literature review:A case report

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World Journal of Clinical Cases 2025年第10期13卷 46-52页

作者： chun-Xin Jiang Guang Yang Lian-ping Shi Peng-Yu Su Department of Cardiovascular North China University of Science and Technology Affiliated HospitalTangshan 063000Hebei ProvinceChina

BACKGROUND Phytosterolemia,also known as sitosterolemia,is a rare autosomal recessive disease characterized by elevated plasma plant sterol levels and xanthomata,which is easily misdiagnosed as familial *** with homozygous phytosterolemia often have severe clinical manifestations,with xanthomata in childhood and premature *** patient had a milder clinical *** SUMMARY This report describes a patient with homozygous phytosterolemia who presented with only elevated cholesterol and low-density lipoprotein cholesterol(LDL-C)without xanthomata,arteriosclerosis,or hematological *** mutation of ABCG5 which encodes an ATP-binding cassette transporter,was detected by whole exome sequencing and diagnosed as *** of the patient’s plasma plant sterol levels detected significant elevations in stigmasterol,rapeseed oil-derived plant sterol,andβ-glutaminol *** was started and a low plant sterol diet was *** patient’s blood lipid profile was reexamined one month later and showed significant decreases in total cholesterol and LDL-C *** has similar clinical features as familial hypercholesterolemia,is highly susceptible to misdiagnosis,and has a very low incidence,and therefore clinicians need to consider a genetic diagnosis of a definitively hyperlipidemic disorder when statin drugs fail to lower lipid *** Phytosterolemia is easily misdiagnosed as familial hypercholesterolaemia and can be treated by dietary modification and cholesterol absorption inhibitors to lower blood lipids.

关键词： Hypercholesterolemia Cholesterol Low-density lipoprotein Phytosterolemia Liquid chromatography ABCG5/ABCG8 Sitosterolemia

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Inhibition controls sensory information processing in cerebellar cortex in mice

Inhibition controls sensory information processing in cerebe...

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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： De-Lai QIU~(1,3,*),chun-ping chu~(2,3) Yan-Hua BING~(1,3) 1 Department of Physiology and Pathophysiology 2 Function Experiment Center College of Basic Medicine 3 Cellular Function Research Center,Yanbian University,Yanji 133000,China

Objective:Although the sensory stimulus precisely evokes response in Purkinje cells(PC),the cellular mechanisms of sensory information processing in cerebellar cortex are *** the present study,we aimed to study the cerebellar PCs in response to natural stimulus on ipsilateral whisker pad by in vivo whole-cell recording ***:Adult(6-8 weeks old) ICR mice were anesthetized with *** a watertight chamber was created around the hemisphere,a craniotomy was drilled to expose the cerebellar surface in correspondence to CrusⅡ.Tactile stimulus on ipsilateral whisker pad was performed by air-puff through a stainless tube connected with a pressurized injection *** potentials and/or currents were monitored with an Axopatch-1D amplifier,acquired through a Digidata 1200 series analog-to-digital interface on a personal computer using Clampex 8.1 *** between the mean values recorded under control and test conditions were evaluated by Student’s paired t-test using SPSS ***:We found that air-puff stimulus on trigeminal afferents evoked neither complex spike nor simple spike,but indeed elicited a strong GABA receptor-mediated inhibition of PCs in cerebellar cortex folium CrusⅡin urethane anesthetized *** potential recordings from both molecular layer and PC layer showed that air-puff stimulus evoked a sequence of parallel fiber volley followed by a GABA receptor-mediated inhibition,which was completely blocked by AMPA receptor ***-attached recordings from PCs showed that air-puff stimulus evoked a pause of spike firing,GABA receptor antagonist abolished the pause,revealed the evoked firing of these PCs in response to the *** current-clamp,air-puff stimulus did not evoke complex spike or simple spike in PCs,but induced strong inhibitory postsynaptic potential (IPSP) in both PCs somata and *** the presence of GABA receptor antagonist,air-puff stimulus could not evoke complex spik

关键词： sensory stimulus cerebellar cortex Purkinje cell in vivo patch-clamp recording inhibition

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基于刻蚀处理的池沸腾传热性能实验研究

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原子与分子物理学报 2025年第3期42卷 180-188页

作者：王菊萍匡纯纯刘妮上海理工大学制冷技术研究所上海200093

为探究刻蚀处理铜板表面对池沸腾传热性能的改善效果,搭建了可视化实验平台,制备不同刻蚀浓度和刻蚀时间处理的表面,采用去离子水为工质,进行饱和池沸腾实验.结果表明,氨水刻蚀浓度越大,表面的换热性能越强,氨水刻蚀时间越长,表面换热... 详细信息

为探究刻蚀处理铜板表面对池沸腾传热性能的改善效果,搭建了可视化实验平台,制备不同刻蚀浓度和刻蚀时间处理的表面,采用去离子水为工质,进行饱和池沸腾实验.结果表明,氨水刻蚀浓度越大,表面的换热性能越强,氨水刻蚀时间越长,表面换热性能越强.热流密度为43.2 W/cm^(2)时,0.05 mol/L氨水刻蚀4 h表面、0.05 mol/L氨水刻蚀5 h表面、0.05 mol/L氨水刻蚀8 h表面、0.03 mol/L氨水刻蚀4 h表面、0.05 mol/L氨水刻蚀4 h表面和0.10 mol/L氨水刻蚀4 h表面上的换热系数分别提高了46%、92%、105%、36%、45%、78%.此外,对其表面进行表征,结果说明了其优异的沸腾性能.刻蚀处理增强了表面粗糙度,有效面积,从而促进液体的补充,能够在较小的过热度下达到更高的临界热流密度.可视化研究结果表明,刻蚀表面具有更多的成核位点,更小的气泡离开直径和离开频率.这些结果显示出刻蚀表面强化池沸腾换热的潜力.

关键词：池沸腾刻蚀传热性能气泡动力学微纳结构表面

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