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Enhanced therapeutic effects for human pancreatic cancer by application K-ras and IGF-IR antisense oligodeoxynucleotides

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World Journal of Gastroenterology 2008年第33期14卷 5176-5185页

作者： Yong-mei Shen Xiao-chun Yang Chen Yang Jun-Kang Shen Radioimmunoassay Center & Clinical Laboratory the Second Affiliated Hospital of Soochow University Suzhou 215004 Jiangsu Province China Department of MRI the Second Affiliated Hospital of Soochow University Suzhou 215004 Jiangsu Province China Radioimmunoassay Center the Municipal Hospital of Suzhou Suzhou 215002 Jiangsu Province China

AIM: To investigate the combined effects of K-ras antisense oligodeoxynucleotide (K-ras ASODN) specif ic to GTT point mutation at codon 12 and type Ⅰ insulin-like growth factor receptor (IGF-IR) antisense oligodeoxynucleotide (IGF-IR ASODN) on proliferation and apoptosis of human pancreatic cancer Patu8988 cells in vitro and in vivo. METHODS: K-ras gene point mutation and its style at codon 12 of human pancreatic cancer cell line Patu8988 were detected by using polymerase chain reaction with special sequence primers (PCR-SSP) and sequence analysis. According to the mutation style, K-ras mutation ASODN specifi c to K-ras point mutation at codon 12 was designed and composed. After K-ras ASODN and IGF-IR ASODN treated on Patu8988 cells respectively or cooperatively, the proliferation and morphological change of Patu8988 cells were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony forming assay andtransmission electron microscopy; the expression of K-ras and IGF-IR mRNA and protein in the treated cells was measured by reverse-transcript polymerase chain reaction (RT-PCR) and flow cytometry respectively; apoptosis was determined by flow cytometry. The combined antitumor activity of K-ras ASODN and IGF-IR ASODN was evaluated in BALB/c nude mice bearing human pancreatic cancer inoculated with Patu8988 cells. RESULTS: The results of PCR-SSP and sequence analysis showed that the human pancreatic cancer cell line Patu8988 had point mutation at codon 12, and the mutation style was GGT→GTT. 2-32 μg/mL K-ras ASODN and 2-32 μg/mL IGF-IR ASODN could inhibit Patu8988 cells' growth, induce apoptosis and decrease the expression of K-ras and IGF-IR mRNA and protein alone. However, there was much more effective inhibition of growth and induction of apoptosis by their combination than by each one alone. In tumor bearing mice, the combination of K-ras ASODN and IGF-IR ASODN showed a signif icant inhibitory effect on the growth of transplanted pa

关键词： Pancreatic cancer Antisense oligodeoxy-nucleotide K-ras Type I insulin-like growth factor receptor Patu8988

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Spheroidizing Behavior and Spheroidizing Kinetics of W-phase During Solid-Solution Treatment in Mg–Zn–Y–Mn–(B)Alloys

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Acta Metallurgica Sinica(English Letters) 2017年第5期30卷 464-469页

作者： Kai Yang Jin-Shan Zhang Xi-mei Zong Wei Liu chun-Xiang Xu College of Materials Science and Engineering TaiyuanUniversity of Technology Taiyuan 030024 China Shanxi Key Laboratory of Advanced Magnesium-based Materials Taiyuan 030024China

The spheroidizing mechanism of W-phase in the Mg–Zn–Y–Mn–（B） alloys during solid-solution treatment was investigated by using kinetic methodologies. The microstructure and mechanical properties of heat-treated Mg_（94）Zn_（2.5）-Y_（2.5）Mn_1 alloy containing 0.003 wt% B were compared with heat-treated Mg_（94）Zn_（2.5）-Y_（2.5）Mn_1 alloy. The heat-treated Mg_（94）Zn_（2.5）-Y_（2.5）Mn_1 alloy with 0.003 wt% B contained fine and uniform W-phase particles, which exhibited optimal mechanical performance. The ultimate tensile strength, yield strength and elongation were 287.7, 125.5 MPa and 21.1%,respectively.

关键词： Magnesium alloys W-phase Spheroidizing Kinetics Microstructure Mechanical properties

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High-drug-loading capacity of redox-activated biodegradable nanoplatform for active targeted delivery of chemotherapeutic drugs

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Regenerative Biomaterials 2020年第4期7卷 359-369页

作者： Hai Zhang Jianqin Yan Heng mei Shengsheng Cai sai Li Furong Cheng Jun Cao Bin He School of Chemical Engineering Sichuan UniversityNo.24 South Section 1Yihuan RoadChengdu 610065China National Engineering Research Center for Biomaterials Sichuan UniversityNo.29 Wangjiang RoadChengdu 610064China

Challenges associated with low-drug-loading capacity,lack of active targeting of tumor cells and unspecific drug release of nanocarriers synchronously plague the success of cancer ***,we constructed active-targeting,redox-activated polymeric micelles(HPGssML)selfassembled aptamer-decorated,amphiphilic biodegradable poly(benzyl malolactonate-co-e-caprolactone)copolymer with disulfide linkage and p-conjugated *** with a homogenous spherical shape and nanosized diameter(-150 nm)formed a low critical micellar concentration(10^-3mg/mL),suggesting good stability of polymeric *** anticancer drug,doxorubicin(DOX),can be efficiently loaded into the core of micelles with high-drug-loading content via strong π-π interaction,which was verified by a decrease in fluorescence intensity and redshift in UV adsorption of DOX in *** redox sensitivity of polymeric micelles was confirmed by size change and in vitro drug release in a reducing *** microscopy and flow cytometry assay demonstrated that conjugating aptamers could enhance specific uptake of HPGssML by cancer *** in vitro cytotoxicity study showed that the half-maximal inhibitory concentration(IC50)of DOX-loaded HPGssML was two times lower than that of the control group,demonstrating improved antitumor ***,the multifunctional biodegradable polymeric micelles can be exploited as a desirable drug carrier for effective cancer treatment.

关键词： good stability,π-conjugated moieties active-targeting,redox-activated

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Reaction condition optimization and kinetic investigation of roasting zinc oxide ore using (NH_4)_2SO_4

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International Journal of Minerals,Metallurgy and Materials 2016年第10期23卷 1133-1140页

作者： Hong-mei Shao Xiao-yi Shen Yi Sun Yan Liu Yu-chun Zhai School of Metallurgy Northeastern University

An orthogonal test was used to optimize the reaction conditions of roasting zinc oxide ore using（NH_4）_2SO_4. The optimized reaction conditions are defined as an（NH_4）_2SO_4/zinc molar ratio of 1.4：1, a roasting temperature of 440°C, and a thermostatic time of 60 min. The molar ratio of（NH_4）_2SO_4/zinc is the most predominant factor and the roasting temperature is the second significant factor that governs the zinc extraction. Thermogravimetric-differential thermal analysis was used for（NH_4）_2SO_4 and zinc mixed in a molar ratio of 1.4：1 at the heating rates of 5, 10, 15, and 20 K·min-1. Two strong endothermic peaks indicate that the complex chemical reactions occur at approximately 290°C and 400°C. XRD analysis was employed to examine the transformations of mineral phases during roasting process. Kinetic parameters, including reaction apparent activation energy, reaction order, and frequency factor, were calculated by the Doyle-Ozawa and Kissinger methods. Corresponding to the two endothermic peaks, the kinetic equations were obtained.

关键词： zinc ore treatment extractive metallurgy kinetic studies reaction mechanisms phase transformation reaction conditions

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Exploiting a depth context model in visual tracking with correlation filter

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Frontiers of Information Technology & Electronic Engineering 2017年第5期18卷 667-679页

作者： Zhao-yun CHEN Lei LUO Da-fei HUANG mei WEN chun-yuan ZHANG College of Computer National University of Defense TechnologyChangsha 410073China National Key Laboratory of Parallel and Distributed Processing Changsha 410073China

Recently correlation filter based trackers have attracted considerable attention for their high computational efficiency. However, they cannot handle occlusion and scale variation well enough. This paper aims at preventing the tracker from failure in these two situations by integrating the depth information into a correlation filter based tracker. By using RGB-D data, we construct a depth context model to reveal the spatial correlation between the target and its surrounding regions. Furthermore, we adopt a region growing method to make our tracker robust to occlusion and scale variation. Additional optimizations such as a model updating scheme are applied to improve the performance for longer video sequences. Both qualitative and quantitative evaluations on challenging benchmark image sequences demonstrate that the proposed tracker performs favourably against state-of-the-art algorithms.

关键词： Visual tracking Depth context model Correlation filter Region growing

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Controllable optical activity of non-spherical Ag and Co SERS substrate with different magnetic field

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Chinese Physics B 2017年第2期26卷 183-188页

作者： chun-Zhen Fan Shuang-mei Zhu Hao-Yi Xin School of Physical Science and Engineering Zhengzhou University Zhengzhou 450052 China College of Science Henan Institute of Engineering Zhengzhou 451191 China

We experimentally fabricate a non-spherical Ag and Co surface-enhanced Raman scattering（SERS） substrate, which not only retains the metallic plasmon resonant effect, but also possesses the magnetic field controllable *** detections are carried out with the test crystal violet（CV） and rhodamine 6G（R6G） molecules with the initiation of different magnitudes of external magnetic field. Experimental results indicate that our prepared substrate shows a higher SERS activity and magnetic controllability, where non-spherical Ag nanoparticles are driven to aggregate effectively by the magnetized Co and plenty of hot-spots are built around the metallic Ag nanoparticles, thereby leading to the enhancement of local electromagnetic field. Moreover, when the external magnetic field is increased, our prepared substrate demonstrates excellent SERS enhancement. With the 2500 Gs and 3500 Gs（1 Gs = 10^（-4）T） magnetic fields, SERS signal can also be obtained with the detection limit lowering down to 10^（-9）M. These results indicate that our proposed magnetic field controlled substrate enables us to freely achieve the enhanced and controllable SERS effect, which can be widely used in the optical sensing, single molecule detection and bio-medical applications.

关键词： magneto-optical materials tunable surface-enhanced Raman scattering

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A novel aptasensor based on 3D-inorganic hybrid composite as immobilized substrate for sensitive detection of platelet-derived growth factor

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Chinese Chemical Letters 2017年第7期28卷 1417-1423页

作者： chun-Ling Zhao mei Hua Can-Yu Yang Yun-Hui Yang College of Chemistry and Chemical Engineering Yunnan Normal UniversityKunming 650092China

A novel electrochemical detection approach for platelet-derived growth factor（PDGF） via ＂sandwich＂structure is reported in this paper. 3D-4MgCO3 Mg（OH）2 4H2O-Au NPs inorganic hybrid composite was utilized as immobilized substrate for sensitive PDGF detection and Pt-Au bimetallic nanoparticles were labelled on PDGF aptamer to indirectly detect PDGF for the first time. The proposed aptasensor exhibited a high catalytic efficiency towards reduction of H2O2, hence the sensitive detection of PDGF was *** showed that the aptasensor exhibited excellent linear response to PDGF, in the range of 0.1 pg/m L–10 ng/m L（4 fmol/L–400 pmol/L）, with detection limit of 0.03 pg/m L（1.2 fmol/L）.

关键词： Aptasensor Platelet-derived growth factor 3D-inorganic hybrid composite Pt-Au bimetallic nanoparticles 4MgCO3·Mg(OH)2·4H2O-Au NPs

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Expression of ECRG4,a novel esophageal cancer-related gene,downregulated by CpG island hypermethylation in human esophageal squamous cell carcinoma

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World Journal of Gastroenterology 2003年第6期9卷 1174-1178页

作者： Shih-HsinLu chun-meiYue Da-JunDeng mei-XiaBi Li-PingGuo DepartmentofEtiologyandCarcinogenesis CancerInstitutePekingUnionMedicalCollegeandChineseAcademyofMedicalSciencesBeijing100021China BeijingInstituteforCancerResearch SchoolofOncologyPekingUniversityBeijing100034China

AIM: To study the mechanisms responsible for inactivation of a novel esophageal cancer related gene 4 (ECRG4) in esophageal squamous cell carcinoma (ESCC).METHODS: A pair of primers was designed to amplify a 220 bp fragment, which contains 16 CpG sites in the core promoter region of the ECRG 4 gene. PCR products of bisulfite-modified CpG islands were analyzed by denaturing high-performance liquid chromatography (DHPLC), which were confirmed by DNA sequencing. The methylation status of ECRG 4 promoter in 20 cases of esophageal cancer and the adjacent normal tissues, 5 human tumor cell lines (esophageal cancer cell line-NEC, EC109, EC9706; gastric cancer cell line- GLC; human embryo kidney cell line-Hek293)and 2 normal esophagus tissues were detected. The expression level of the ECRG 4 gene in these samples was examined by RT-PCR.RESULTS: The expression level of ECRG 4 gene was *** 20 esophageal cancer tissues, nine were unexpressed,six were lowly expressed and five were highly expressed compared with the adjacent tissues and the 2 normal esophageal epithelia. In addition, 4 out of the 5 human cell lines were also unexpressed. A high frequency of methylation was revealed in 12 (8 unexpressed and 4 lowly expressed)of the 15 (80%) downregulated cancer tissues and 3 of the 4 unexpressed cell lines. No methylation peak was observed in the two highly expressed normal esophageal epithelia and the methylation frequency was low (3/20) among the 20 cases in the highly expressed adjacent tissues. The methylation status of the samples was consistent with the result of DNA sequencing.CONCLUSION: These results indicate that the inactivation of ECRG 4gene by hypermethylation is a frequent molecular event in ESCC and may be involved in the carcinogenesis of this cancer.

关键词：食管癌鳞癌 ECRG4 基因表达肿瘤病因学

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Ortho-u-monoids

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Acta Mathematica Sinica,English Series 2011年第5期27卷 831-844页

作者： chun mei GONG Yu Qi GUO K. P. SHUM Department of Mathematics Southwest University Chongqing 400715 P. R. China Department of Mathematics Xi'an University of Architecture and Technology Xi'an 7'10055 P. R. China Department of Mathematics The University of Hong Kong Hong Kong P. R. China

In this paper, we study the class of ortho-u-monoids which are generalized orthogroups within the class of E（S）-semiabundant semigroups. After introducing the concept of （-）-Green＇s relations, and obtaining some important properties of （-）-Green＇s relations and super E（S）-semiabundant semigroups, we have given the semilattice decomposition of ortho-u-monoids and a structure theorem for regular ortho-u-monoids. The main techniques that we used in the study are the （-）-Green＇s relations, and the semi-spined product of semigroups.

关键词： (-)-Green's relations super E(S)-semiabundant semigroups ortho-u-monoids semi-spin- ed products

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Estimating the properties of weathered bedrock and pile-rock interaction from the geological strength index

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Journal of Mountain Science 2018年第8期15卷 1757-1776页

作者： ZHOU chun-mei SHAO Wei YIN Kun-long YANG Zong-ji School of Civil Engineering and architecture Wuhan Institute of Technology Key laboratory of Meteorological Disaster Ministry of Education/Joint International Research Laboratory of Climate and Environment Change/Collaborative Innovation Centre on Forecast and Evaluation of Meteorological Disasters/School of Hydrology and Water Resources Nanjing University of Information Science and Technology Faculty of Engineering China University of Geosciences Key Laboratory of Mountain Hazards and Surface Processes Institute of Mountain Hazards and Environment Chinese Academy of Sciences

The effect of variable rock mass properties on pile-rock interaction poses a great challenge to the design of stabilizing piles and numerical analysis of pile-rock interaction. The paper presents a novel method to estimate the properties of weathered bedrock, which can be applied to routine design of landslide-stabilizing piles for collivial landslides. The Ercengyan landslide located in the Three Gorges Reservoir, China, is the area of interest for this study. A geological investigation and triaxial tests were conducted to estimate the basic parameters, including Geological Strength Index(GSI), uniaxial compressive strength σ_(ci) and Hoek-Brown constant m_i of intact bedrock in the study area. Hoek-Brown criterion was used to estimate mechanical properties of the weathered rock, including elastic modulus E_m, cohesion c, friction angle Φ, and normal ultimate lateral resistance p_(max). A parametric study was performed to evaluate the effect of parameterizations of GSI, σ_(ci) and m_i on the bedrock properties and p-y curves. The estimated rock mass properties were used with PLAXIS 2D software to simulate pile-rock interaction. Effect of GSI on stress at the pile-rock interface and in the rock, pile bending moment, pile shear force, and p-y curve were analysed.

关键词： Landslide Stabilizing pile Weathered rock Pile-rock interaction Geological strength index

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