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The importance of a potential phosphorylation site in enamelin on enamel formation

International Journal of Oral Science 2017年第4期9卷 210-215页

作者： Wen-Juan Yan Pan Ma Ye Tian Jing-Ya Wang chun-lin qin Jian Q Feng Xiao-Fang Wang Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis Texas A&M University College of DentistryDallasUSA Department of Endodontics Nanfang HospitalSouthern Medical UniversityGuangzhouChina

Enamelin （ENAM） has three putative phosphoserines （pSers） phosphorylated by a Golgi-associated secretory pathway kinase （FAM20C） based on their distinctive Ser-x-Glu （S-x-E） motifs. Fam2OC-knockout mice show severe enamel defects similar to those in the Enam-knockout mice, implying an important role of the pSers in ENAM. To determine the role of pSer5s in ENAM, we characterized ENAMRgsc514 mice, in which Sers5 cannot be phosphorylated by FAM20C due to an E57〉Gs7 mutation in the S-x-E motif, The enamel microstructure of 4-week-old mice was examined by scanning electron microscopy. The teeth of 6-day-old mice were characterized by histology and immunohistochemistry. The protein lysates of the first lower molars of 4-day-old mice were analyzed by Western immunoblotting using antibodies against ENAM, ameloblastin and amelogenin. ENAMRgsc514 heterozygotes showed a disorganized enamel microstructure, while the homozygotes had no enamel on the dentin surface. The N-terminal fragments of ENAM in the heterozygotes were detained in the ameloblasts and localized in the mineralization front of enamel matrix, while those in the WT mice were secreted out of ameloblasts and distributed evenly in the outer 1/2 of enamel matrix. Surprisingly, the 15 kDa C-terminal fragments of ameloblastin were not detected in the molar lysates of the homozygotes. These results suggest that the phosphorylation of SerSS may be an essential posttranslational modification of ENAM and is required for the interaction with other enamel matrix molecules such as ameloblastin in mediating the structural organization of enamel matrix and protein-mineral interactions during enamel formation.

关键词： ameloblastin enamel enamelin FAM20C phosphorylation phosphoserine

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Loss of epithelial FAM20A in mice causes amelogenesis imperfecta, tooth eruption delay and gingival overgrowth

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International Journal of Oral Science 2016年第2期8卷 98-109页

作者： Li-Li Li Pei-Hong Liu Xiao-Hua Xie Su Ma Chao Liu Li Chen chun-lin qin Harbin Medical University School of Stomatology Harbin China Department of Stomatology the Second Affiliated Hospital of Harbin Medical University Harbin China Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis Texas A&M University Baylor College of Dentistry Dallas USA Longjiang Scholar Laboratory the First Affiliated Hospital of Harbin Medical University Harbin China

FAM20A has been studied to a very limited extent. Mutations in human FAM20A cause amelogenesis imperfecta, gingival fibromatosis and kidney problems. It would be desirable to systemically analyse the expression of FAM20A in dental tissues and to assess the pathological changes when this molecule is specifically nullified in individual tissues. Recently, we generated mice with a Fam2OA-floxed allele containing the beta-galactosidase reporter gene. We analysed FAM20A expression in dental tissues using X-Gal staining, immunohistochemistry and in situ hybridization, which showed that the ameloblasts in the mouse mandibular first molar began to express FAM20A at 1 day after birth, and the reduced enamel epithelium in erupting molars expressed a significant level of FAM2OA. By breeding K14-Cre mice with Fam20An^x/fl^x mice, we created K14-Cre;Fam20Af/flox/flox （conditional knock out, cKO） mice, in which Fam20A was inactivated in the epithelium. We analysed the dental tissues of cKO mice using X-ray radiography： histology and immunohistochemistry. The molar enamel matrix in cKO mice was much thinner than normal and was often separated from the dentinoenamel junction. The Fam2OA-deficient ameloblasts were non-polarized and disorganized and were detached from the enamel matrix. The enamel abnormality in cKO mice was consistent with the diagnosis of amelogenesis imperfecta. The levels of enamelin and matrix metalloproteinase 20 were lower in the ameloblasts and enamel of cKO mice than the normal mice, The cKO mice had remarkable delays in the eruption of molars and hyperplasia of the gingival epithelium. The findings emphasize the essential roles of FAM20A in the development of dental and oral tissues.

关键词： conditional knock out mice enamel FAM2OA gingival overgrowth tooth eruption

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Dentin matrix protein 1 and phosphate homeostasis are critical for postnatal pulp, dentin and enamel formation

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International Journal of Oral Science 2012年第4期4卷 189-195页

作者： Afsaneh Rangiani Zheng-Guo Cao Ying Liu Anika Voisey Rodgers Yong Jiang chun-lin qin Jian-Quan Feng Biomedical Sciences Baylor College of DentistryTexas A&M Health Science Center

Deletion or mutation of dentin matrix protein 1 （DMP1） leads to hypophosphatemic rickets and defects within the dentin. However, it is largely unknown if this pathological change is a direct role of DMP1 or an indirect role of phosphate （Pi） or both. It has also been previously shown that Klotho-deficient mice, which displayed a high Pi level due to a failure of Pi excretion, causes mild defects in the dentinal structure. This study was to address the distinct roles of DMP1 and Pi homeostasis in cell differentiation, apoptosis and mineralization of dentin and enamel. Our working hypothesis was that a stable Pi homeostasis is critical for postnatal tooth formation, and that DMP1 has an antiapoptotic role in both amelogenesis and dentinogenesis. To test this hypothesis, Dmpl-null （Dmpl-/-）, Klotho-deficient （kl/kl）, Dmpl/Klotho-double-deficient （Dmpl-/-/kl/kl） and wild-type （WT） mice were killed at the age of 6 weeks. Combinations of X-ray, microcomputed tomography （I^CT）, scanning electron microscopy （SEM）, histology, apoptosis and immunohistochemical methods were used for characterization of dentin, enamel and pulp structures in these mutant mice. Our results showed that Dmpl-/- （a low Pi level） or kl/kl（a high Pi level） mice displayed mild dentin defects such as thin dentin and a reduction of dentin tubules. Neither deficient mouse line exhibited any apparent changes in enamel or pulp structure. However, the double-deficient mice （a high Pi level） displayed severe defects in dentin and enamel structures, including loss of dentinal tubules and enamel prisms, as well as unexpected ectopic ossification within the pulp root canal. TUNEL assay showed a sharp increase in apoptotic cells in ameloblasts and odontoblasts. Based on the above findings, we conclude that DMP1 has a protective role for odontoblasts and ameloblasts in a pro-apoptotic environment （a high Pi level）.

关键词： apoptosis dentin dentin matrix protein 1 enamel Klotho phosphate

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Characterization of Fam20C expression in odontogenesis and osteogenesis using transgenic mice

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International Journal of Oral Science 2015年第2期7卷 89-94页

作者： Er-Xia Du Xiao-Fang Wang Wu-Chen Yang Deborah Kaback Siu-Pok Yee chun-lin qin Anne George Jian-Jun Hao Division of Orthodontics Department of Craniofacial SciencesSchool of Dental MedicineUniversity of Connecticut Department of Biomedical Sciences Texas A&M Health Science CenterBaylor College of Dentistry Department of Genetics and Developmental Biology University of Connecticut Health Center Department of Oral Biology University of Illinois

Our previous studies have demonstrated that Fam20 C promotes differentiation and mineralization of odontoblasts,ameloblasts,osteoblasts and osteocytes during tooth and bone *** of the Fam20 C gene inhibits bone and tooth growth by increasing fibroblast growth factor 23 in serum and causing hypophosphatemia in conditional knockout ***,control and regulation of the expression of Fam20 C are still *** this study,we generated a transgenic reporter model which expresses green fluorescence protein（GFP） driven by the Fam20 C *** was used to insert a 16 kb fragment of the mouse Fam20 C gene（containing the 15 kb promoter and 1.1 kb of exon 1） intoa pBluescript SK vector with the topaz variant of GFP and a bovine growth hormone polyadenylation *** expression was subsequently evaluated by histomorphometry on cryosections from E14 to adult *** was evident in the bone and teeth as early as *** GFP signal was maintained stably in odontoblasts and osteoblasts until 4 weeks after *** expression of GFP was significantly reduced in teeth,alveolar bone and muscle by 8 weeks of *** also observed colocalization of the GFP signal with the Fam20 C antibody in postnatal 1- and 7-day-old *** generation of Fam20C-GFP transgenic mice will provide a unique model for studying Fam20 C gene expression and the biological function of this gene during odontogenesis and osteogenesis.

关键词： transgenic promoter pBluescript osteogenesis teeth transgene reporter Fluorescence insert alveolar

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Electrocatalytic cleavage of a lignin β-O-4 model compound and coupling with nitrogen-containing aromatics using Prussian blue analogue-derived nickel–cobalt spinel

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Journal of Energy Chemistry 2025年第1期100卷 628-636页

作者： Yi Qi Xueying Chen Bowen Liu Xuliang lin Xueqing Qiu Yanlin qin Guangdong Provincial Key Laboratory of Plant Resources Biorefinery School of Chemical Engineering and Light Industry Guangdong University of Technology Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Guangdong Basic Research Center of Excellence for Ecological Security and Green Development Guangdong University of Technology

Electrochemical conversion of lignin for the production of high-value heterocyclic aromatic compounds has great *** demonstrate the targeted synthesis and cation modulation of NiCo2O4spinel nanoboxes,synthesized via cation exchange and calcination *** catalysts exhibit excellent efficacy in the electrocatalytic conversion of lignin model compounds,specifically 2-phenoxy-1-phenylethanol,into nitrogen-containing aromatics,achieving high conversion rates and *** catalysts were synthesized via a cation exchange and calcination oxidation process,using Prussian blue nanocubes as *** porous architecture and polymetallic composition of the NiCo2O4spinel demonstrated superior performance in electrocatalytic oxidative coupling,achieving a 99.2 wt%conversion rate of the 2-phenoxy-1-phenylethanol with selectivities of 37.5 wt%for quinoline derivatives and 31.5 wt%for *** innovations include the development of a sustainable one-pot synthesis method for quinoline derivatives,the elucidation of a multistage reaction pathway involving CAO bond cleavage,hydroxyaldol condensation,and CAN bond formation,and a deeper mechanistic understanding derived from DFT *** work establishes a new strategy for lignin valorization,offering a sustainable route to produce high-value nitrogen-containing aromatics from renewable biomass under mild conditions,without the need for additional reagents.

关键词： Spinel Biomass valorization Electrocatalysis Lignin oxidation Coupling

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咪唑立宾治疗儿童原发性肾病综合征临床进展

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中国实用儿科杂志 2015年第8期30卷 629-633页

作者：林春琴余自华南京军区福州总医院儿科福建福州350025

原发性肾病综合征(primary nephrotic syndrome,PNS)是儿童常见肾小球疾病。糖皮质激素(GC)是治疗PNS的一线药物。根据对GC的反应,PNS可分为激素敏感型肾病综合征(steroid-sensitive nephroticsyndrome,SSNS)和激素耐药型肾病综合征(ste... 详细信息

关键词：咪唑立宾原发性肾病综合征激素敏感型激素耐药型儿童

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Non-coding RNAs in acute ischemic stroke:from brain to periphery

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Neural Regeneration Research 2025年第1期20卷 116-129页

作者： Shuo Li Zhaohan Xu Shiyao Zhang Huiling Sun Xiaodan qin lin Zhu Teng Jiang Junshan Zhou Fuling Yan Qiwen Deng Department of Neurology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Neurology Zhongda HospitalSchool of MedicineSoutheast UniversityNanjingJiangsu ProvinceChina General Clinical Research Center Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina

Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and *** predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain *** innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental *** expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic ***,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and *** particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic *** this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and *** from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.

关键词： acute ischemic stroke apoptosis blood–brain barrier damage circular RNAs excitatory toxicity long non-coding RNAs microRNAs neuroinflammation non-coding RNAs oxidative stress

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盐酸替罗非班清洁验证残留量测定方法的研究

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时珍国医国药 2013年第1期24卷 31-32页

作者：平丽林春琴盖仁华浙江大学药物安全评价研究中心浙江杭州310058

目的建立生产系统中盐酸替罗非班清洁后残留量的测定方法,保证各设备的清洁效果。方法采用高效液相色谱法,以0.01 mol/L的磷酸二氢钾缓冲液(用磷酸调节pH至2.3)-乙腈(73∶27)为流动相,227 nm为检验波长,1.0 ml/min为流速进行测定。结果... 详细信息

目的建立生产系统中盐酸替罗非班清洁后残留量的测定方法,保证各设备的清洁效果。方法采用高效液相色谱法,以0.01 mol/L的磷酸二氢钾缓冲液(用磷酸调节pH至2.3)-乙腈(73∶27)为流动相,227 nm为检验波长,1.0 ml/min为流速进行测定。结果盐酸替罗非班在0.075～0.75μg/ml之间峰面积与浓度呈良好的线性关系(r=0.999 7),平均取样回收率为92.45%。结论该分析方法可用于盐酸替罗非班清洁验证的残留物检测。

关键词：盐酸替罗非班残留量测定

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可燃性气体检测报警系统的研究

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齐齐哈尔大学学报（自然科学版） 2003年第2期19卷 38-40页

作者：李春林齐怀琴齐齐哈尔大学信息学院齐齐哈尔161006

本文研究了一种以热线型半导体式传感器为检测元件且带补救措施的可燃性气体报警系统。该系统采用高灵敏度的传感器对可燃性气体进行检测,可以快速而准确地反映现场状况,并有声音报警、指示报警及相关数据显示,而且显示的数据也有利于... 详细信息

本文研究了一种以热线型半导体式传感器为检测元件且带补救措施的可燃性气体报警系统。该系统采用高灵敏度的传感器对可燃性气体进行检测,可以快速而准确地反映现场状况,并有声音报警、指示报警及相关数据显示,而且显示的数据也有利于报警的善后处理。

关键词：可燃性气体传感器报警控制系统

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全寿命周期成本方法在电网工程设计中的运用

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电力勘测设计 2010年第2期22卷 63-66页

作者：林春钦林章岁周加和福建省电力勘测设计院福建福州350003

探讨了全寿命周期成本方法在输变电工程设计中的应用,建立了相应的计算模型。分析了全寿命周期成本方法应用中的若干问题,如计算基础数据难以获取、缺乏具体设备和材料的计算参数等。为了更加科学合理应用全寿命周期成本方法进行电网工... 详细信息

探讨了全寿命周期成本方法在输变电工程设计中的应用,建立了相应的计算模型。分析了全寿命周期成本方法应用中的若干问题,如计算基础数据难以获取、缺乏具体设备和材料的计算参数等。为了更加科学合理应用全寿命周期成本方法进行电网工程设计评价,必须统一和规范边界条件和计算参数。为此,提出了应用全寿命周期成本方法的具体措施建议。

关键词：全寿命周期成本计算模型计算参数措施建议

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