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In vitro Effects of Nerve Growth Factor on Cardiac Fibroblasts Proliferation, Cell Cycle, Migration, and Myofibroblast Transformation

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Chinese Medical Journal 2018年第7期131卷 813-817页

作者： Yong Zhao chun-hua ding Department of Cardiology Zhongshan Hospital Affiliated with Guangzhou University of Chinese Medicine Zhongshan Guangdong 528400 China Department of Cardiology Aerospace Center Hospital Peking University Aerospace Clinical College of Medicine Beijing 100049 China

Background： Recent research indicates that nerve growth factor （NGF） promotes cardiac repair following myocardial infarction by promoting angiogenesis and cardiomyocyte survival. The purpose of this study was to investigate the effects of NGF on cardiac fibroblasts （CFs） proliferation, cell cycle, migration, and myofibroblast transformation in vitro. Methods： CFs were obtained from ventricles of neonatal Sprague-Dawley rats and incubated with various concentrations of NGF （0, 0.01,0.1, 1, 10, and 100 ng/ml; 0 ng/ml was designated as the control group）. Cell proliferation and cell cycle of the CFs were measured by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay and flow cytometry （FCM）, respectively. A cell scratch wound model and transwell were carried out to observe effects of NGF on migration of CFs after 24 h of culture. Real-time polymerase chain reaction （RT-PCR） and Western blotting were used to measure a-smooth muscle actin （α-SMA） at mRNA and protein levels after CFs were incubated with various concentrations of NGF. Results： Expression of α-SMA measured by RT-PCR and Western blotting significantly increased in the 1 and 10 ng/ml NGF groups （P 〈 0.05）. Absorbance values of CFs showed that NGF did not influence the proliferation of CFs （The A490values were 0.178 ± 0.038, 0.182 ± 0.011,0.189 ± 0.005, 0. 178 ± 0.01 0, 0.185± 0.025, and 0.177 ± 0.033, respectively, in the 0, 0.01,0.1, 1, 10, and 100 ng/ml NGF groups [P = 0.800, 0.428, 0.981, 0.596, and 0.913, respectively, compared with control group]）, and FCM analysis showed that the percentage of CFs in G0/G1, S, and G2/M phases was not changed （P 〉 0.05）. The cell scratch wound model and transwell showed that CFs migration was not significantly different （P 〉 0.05）. Conclusion： NGF induces myofibroblast transformation but does not influence proliferation, cell cycle, or migration of CFs in vitro

关键词： Cell Cycle Cell Movement Cell Proliferation Fibroblasts Myofibroblasts Nerve Growth Factor

Biatrial Macroreentry Atrial Tachycardia after Atria Fibrillation Ablation

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Chinese Medical Journal 2016年第18期129卷 2250-2252页

作者： Shen huang Yu-Mei Xue Wen-Chang Zhang chun-hua ding Qi-Yan Li Xian-Zhang Zhan Arrhythmia Center Guangdong Provincial Hospital of Chinese Medicine (The Second Affiliated Hospital of Guangzhou University of Chinese Medicine) Guangzhou Guangdong 510006 China Department of Cardiology Guangdong Cardiovascular Institute Guangdong General Hospital Guangdong Academy of Medical Sciences Guangzhou Guangdong 510080 China

Catheter ablation is an important therapy for atrial fibrillation （AF） in the last decade. In parallel, atrial tachycardia （AT） has become the most common type of arrhythmia after AF ablation, especially after extensive left atrial （LA） substrate modification,t^j The occurrence of AT after AF is due to the conduction gaps of ablation lines and the conduction obstacle caused by the ablation lesions？-~1 Most of these ATs locate in LA, and here, we described a biatrial macroreentry AT （MAT） after AF ablation.

关键词： Activation Mapping Atrial Tachycardia Catheter Ablation

Inhibiting SHP2 reduces glycolysis, promotes microglial M1 polarization, and alleviates secondary inflammation following spinal cord injury in a mouse model

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Neural Regeneration Research 2025年第3期20卷 858-872页

作者： Xintian ding chun Chen Heng Zhao Bin Dai Lei Ye Tao Song Shuai huang Jia Wang Tao You Department of Orthopedics The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Orthopedics Provincial Hospital Affiliated to Anhui Medical UniversityHefeiAnhui ProvinceChina

Reducing the secondary inflammatory response, which is partly mediated by microglia, is a key focus in the treatment of spinal cord injury. Src homology 2-containing protein tyrosine phosphatase 2(SHP2), encoded by PTPN11, is widely expressed in the human body and plays a role in inflammation through various mechanisms. Therefore, SHP2 is considered a potential target for the treatment of inflammation-related diseases. However, its role in secondary inflammation after spinal cord injury remains unclear. In this study, SHP2 was found to be abundantly expressed in microglia at the site of spinal cord injury. Inhibition of SHP2 expression using siRNA and SHP2 inhibitors attenuated the microglial inflammatory response in an in vitro lipopolysaccharide-induced model of inflammation. Notably, after treatment with SHP2 inhibitors, mice with spinal cord injury exhibited significantly improved hind limb locomotor function and reduced residual urine volume in the bladder. Subsequent in vitro experiments showed that, in microglia stimulated with lipopolysaccharide, inhibiting SHP2 expression promoted M2 polarization and inhibited M1 polarization. Finally, a co-culture experiment was conducted to assess the effect of microglia treated with SHP2 inhibitors on neuronal cells. The results demonstrated that inflammatory factors produced by microglia promoted neuronal apoptosis, while inhibiting SHP2 expression mitigated these effects. Collectively, our findings suggest that SHP2 enhances secondary inflammation and neuronal damage subsequent to spinal cord injury by modulating microglial phenotype. Therefore, inhibiting SHP2 alleviates the inflammatory response in mice with spinal cord injury and promotes functional recovery postinjury.

关键词： apoptosis glycolysis inflammatory response microglia neurons polarization spinal cord injury Src homology 2-containing protein tyrosine phosphatase 2

Low‑Temperature Oxidation Induced Phase Evolution with Gradient Magnetic Heterointerfaces for Superior Electromagnetic Wave Absorption

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Nano-Micro Letters 2025年第1期17卷 191-204页

作者： Zizhuang He Lingzi Shi Ran Sun Lianfei ding Mukun He Jiaming Li hua Guo Tiande Gao Panbo Liu School of Chemistry and Chemical Engineering Northwestern Polytechnical UniversityXi’an 710129People’s Republic of China School of Marine Science and Technology Northwestern Polytechnical UniversityXi’an 710072People’s Republic of China

Gradient magnetic heterointerfaces have injected infinite vitality in optimizing impedance matching,adjusting dielectric/magnetic resonance and promoting electromagnetic(EM)wave absorption,but still exist a significant challenging in regulating local phase ***,accordion-shaped Co/Co_(3)O_(4)@N-doped carbon nanosheets(Co/Co_(3)O_(4)@NC)with gradient magnetic heterointerfaces have been fabricated via the cooperative high-temperature carbonization and lowtemperature oxidation *** results indicate that the surface epitaxial growth of crystal Co_(3)O_(4) domains on local Co nanoparticles realizes the adjustment of magnetic-heteroatomic components,which are beneficial for optimizing impedance matching and interfacial ***,gradient magnetic heterointerfaces simultaneously realize magnetic coupling,and long-range magnetic ***,the synthesized Co/Co_(3)O_(4)@NC absorbents display the strong electromagnetic wave attenuation capability of−53.5 dB at a thickness of 3.0 mm with an effective absorption bandwidth of 5.36 GHz,both are superior to those of single magnetic domains embedded in carbon *** design concept provides us an inspiration in optimizing interfacial polarization,regulating magnetic coupling and promoting electromagnetic wave absorption.

关键词： Magnetic heterointerfaces Phase evolution Interfacial polarization Magnetic coupling Electromagnetic wave absorption

Hf_(2)CF_(2)单层热传输及热电性质的第一原理研究

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原子与分子物理学报 2025年第4期42卷 168-176页

作者：董文欣李铁平张莉丁迎春何开华中国地质大学(武汉)数学与物理学院武汉430074 宜宾学院材料与化学工程学部宜宾644000

二维MXene材料是一种新型的功能性材料,其在热电领域具有潜在应用价值.本文采用第一性原理计算方法,通过求解声子和电子的玻尔兹曼输运方程,计算研究了MXene Hf_(2)CF_(2)单层的热传输特征和热电性质.声子谱揭示了Hf_(2)CF_(2)单层具有... 详细信息

二维MXene材料是一种新型的功能性材料,其在热电领域具有潜在应用价值.本文采用第一性原理计算方法,通过求解声子和电子的玻尔兹曼输运方程,计算研究了MXene Hf_(2)CF_(2)单层的热传输特征和热电性质.声子谱揭示了Hf_(2)CF_(2)单层具有热力学稳定性.在室温条件下,Hf_(2)CF_(2)单层的晶格热导率为14.25 Wm^(-1)K^(-1),且随着温度的升高而降低;电子热导率却随着温度和掺杂浓度的增加而升高.电输运性质中,电导率和Seebeck系数随载流子浓度的变化相反,确保能够获得优异的热电性能.在600 K条件下,n型MXene Hf_(2)CF_(2)单层的最大功率因子可以达到61.24 mW/(mK^(2)).载流子迁移率具有较高的各向异性,且电子的迁移率远远高于空穴的迁移率.基于热导及电导计算结果,n型MXene Hf_(2)CF_(2)单层沿x轴方向的热电优值可达到1.75,高于其他已报道的类似结构的热电优值,表明了MXene Hf_(2)CF_(2)单层是一种潜在的高性能n型热电材料.

关键词： MXene Hf_(2)CF_(2) 热电性能晶格热导率第一性原理

Curcumin Reduces Cardiac Fibrosis by Inhibiting Myofibroblast Differentiation and Decreasing Transforming Growth Factor β1 and Matrix Metalloproteinase 9/Tissue Inhibitor of Metalloproteinase 1

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Chinese Journal of Integrative Medicine 2017年第5期23卷 362-369页

作者： MA Jin MA Shi-yu ding chun-hua Cardiac Electrophysiology Research Lab Guangdong Provincial Academy of Chinese Medical Sciences Guangzhou 510006 China Department of Critical Care Medicine Guangdong Provincial Hospital of Chinese Medicine Guangzhou 510006 China Arrhythmia Center Guangdong Provincial Hospital of Chinese Medicine Guangzhou 510006 China

Objective： To study the effect of curcumin on fibroblasts in rats with cardiac fibrosis. Methods： The rats were randomly divided into 4 groups（n=12 in each group）： the normal control, isoproterenol（ISO）, ISO combined with low-dose curcumin（ISO＋Cur-L）, and ISO combined with high-dose curcumin（ISO＋Cur-H） groups. ISO＋Cur-L and ISO＋Cur-H groups were treated with curcumin（150 or 300 mg·kg-1·day-1） for 28 days. The primary culture of rat cardiac fibroblast was processed by trypsin digestion method in vitro. The 3rd to 5th generation were used for experiment. Western blot method was used to test the expression of collagen type Ⅰ/Ⅲ, α-smooth muscle actin（α-SMA）, transforming growth factor（TGF）-β1, matrix metalloproteinase（MMP）-9 and tissue inhibitor of metalloproteinase（TIMP）-1. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetr-azolium bromide（MTT） assay was applied to test the proliferation of fibroblast. Result： Curcumin significantly decreased interstitial and perivascular myocardial collagen deposition and cardiac weight index with reducing protein expression of collagen type Ⅰ/Ⅲ in hearts（P〈0.05）. In addition, curcumin directly inhibited angiotensin（Ang） Ⅱ-induced fibroblast proliferation and collagen type Ⅰ/Ⅲ expression in cardiac fibroblasts（P〈0.05）. Curcumin also inhibited fibrosis by inhibiting myofibroblast differentiation, decreased TGF-β1, MMP-9 and TIMP-1 expression（P〈0.05） but had no effects on Smad3 in Ang Ⅱ incubated cardiac fibroblasts. Conclusions： Curcumin reduces cardiac fibrosis in rats and Ang Ⅱ-induced fibroblast proliferation by inhibiting myofibroblast differentiation, decreasing collagen synthesis and accelerating collagen degradation through reduction of TGF-β1, MMPs/TIMPs. The present findings also provided novel insights into the role of curcumin as an anti-fibrotic agent for the treatment of cardiac fibrosis.

关键词： curcumin fibrosis myofibroblast transforming growth factor matrix metalloproteinase

Advances in ethnopharmacology,phytochemistry and pharmacology of Erigerontis Herba and its Chinese medicine prescriptions

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Traditional Medicine Research 2025年第1期10卷 44-61页

作者： Yang Zhou Yuan-Yan huang ding-Yue Shang Ying Chen Meng Tian Cheng-Yu Jin Xin Lu Bing-chun Yan Han-Qing Pang Institute of Translational Medicine School of MedicineYangzhou UniversityYangzhou 225009China Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Yangzhou UniversityYangzhou 225009China School of Medicine Nanjing University of Chinese MedicineNanjing 210023China State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources Guangxi Normal UniversityGuiling 541004China.

Erigerontis Herba(EH),the dried whole plant of Erigeron breviscapus,is well-known for circulating blood,activating meridians to alleviate pain,expelling wind,and clearing away *** has been extensively utilized in southern China for the treatment of stroke hemiplegia,chest stuffiness and pains,rheumatic arthralgia,headache,and *** review focuses on the botany,ethnopharmacology,phytochemistry,pharmacology and toxicity of EH and its related prescriptions to offer new insights for prospective research of *** information about EH was retrieved from ancient records and books,PubMed,China National Knowledge Infrastructure,Chinese Pharmacopoeia,Web of Science,Doctoral and Master’s Theses,and various electronic *** is a member of Compositae family and is mainly grown in southern *** Chinese medicine records that EH has the effects of circulating blood and removing blood stasis,expelling wind,and removing cold,as well as relieving rigidity of muscle and relieving *** now,nearly 200 ingredients have been characterized from EH,including flavonoids,caffeoyls,aromatic acids,coumarins,pentacyclic terpenoids,volatile oil and other *** extracts,EH related prescriptions(Dengzhan Xixin injection,Dengzhan Shengmai capsules,etc.)or compounds(scutellarin,scutellarein,etc.)possessed obvious therapeutic effects of ischemic stroke,cerebral hemorrhage,myocardial infarction,Alzheimer’s disease,diabetes and its complications,gastric cancer,bone,and joint degenerative ***,the major active compound of EH,has been used as a quality *** no obvious toxicity of EH has been *** to its traditional applications,ethnopharmacology,phytochemistry,pharmacology,and toxicity,EH was applied as a valuable herb for clinical application in food and medicine *** several compounds have been shown to possess diverse biological activities,the underlying mechanisms of their actions remain *** fully exploi

关键词： Erigeron breviscapus traditional applications ethnopharmacology phytochemistry pharmacology

Laser Light Scattering Study on Aggregation of Cellulose Diacetate in Acetone

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Chinese Journal of Chemical Physics 2014年第3期27卷 256-258页

作者： ding-hai Xie Xu-hua Li Xi-ling Fang chun-feng Ma Technology Center China Tobacco Guangdong Industrial Co. Ltd. Guangzhou 510385 China Faculty of Materials Science and Engineering South China University of Technology Guangzhou 510540 China

We have investigated the properties of cellulose diacetate in solution by using laser light scattering. The cellulose diacetate molecules can form micelles and micellar clusters in acetone besides the individual chains. As the concentration increases, the average hydrodynamic radius （Rh） linearly increases, whereas the ratio of gyration radius to hydrodynamic radins 〈Rg〉/〈Rh〉 linearly decreases. It indicates that the micelles associate and form micellar clusters due to the intermolecular interactions.

关键词： Cellulose diacetate Laser light scattering Multi-aggregation form structure

Performance of X-Section Concrete Pile Group in Coral Sand Under Vertical Loading

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China Ocean Engineering 2020年第5期34卷 621-630页

作者： PENG Yu LIU Jia-yi ding Xuan-ming FANG hua-qiang JIANG chun-yong College of Civil Engineering Chongqing UniversityChongqing 400045China Key Laboratory of New Technology for Construction of Cities in Mountain Area Chongqing UniversityChongqing 400045China CCTEG Chongqing Engineering Co. LtdGeotechnical Engineering InstituteChongqing 400042China

To reveal the bearing capacity of the X-section pile group in coral sand, a series of model load tests are *** testing results are presented as load-settlement curves, pile-soil stress ratios, distributions of side friction and axial force, and load-sharing ratio between side and tip resistances. The reliability and accuracy of the numerical simulation model are verified by comparing the results of the model test. Comparative analysis between X-section and circular section piles with the same cross-sectional area indicates that the bearing capacity of the X-section pile group is much larger than that of the circular pile group. The axial force of X-section piles is smaller while the peak skin friction is larger than that of circular piles at the same depth. The skin friction of the core pile is the largest,followed by the side pile and the corner pile is the smallest when the load is relatively small;however, it is converse when the load is larger than 10 k N. Compared with piles in silica sand, the pile in coral sand has a lower bearing capacity, and the sand breakage leads to the steep drop failure of pile foundation. Moreover, pile positions under the raft have less effect on the load-share differences among corner, side and core piles in coral sand. This study provides a reference for the construction of pile foundations in coral sand.

关键词： X-section pile group coral sand model test numerical analysis pile-soil stress ratio