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上海市虹口区中小学生麻疹疫苗强化免疫效果分析

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上海预防医学 2014年第3期26卷 113-115,127页

作者：李淑华解范迪吴春珠钱晓华汤素文杨彦基于晓楠汤显上海市虹口区疾病预防控制中心上海200082 上海市虹口区江湾医院上海200434 上海市疾病预防控制中心上海200336

[目的]从免疫学和流行病学两个方面评价上海市虹口区6～14岁学龄儿童麻疹疫苗强化免疫的效果。[方法]强化免疫1个月后采集静脉血，采用ELISA法测定麻疹IgG抗体，与未参加强化免疫者的抗体水平进行比较。同时，收集研究个体的麻疹免疫史... 详细信息

[目的]从免疫学和流行病学两个方面评价上海市虹口区6～14岁学龄儿童麻疹疫苗强化免疫的效果。[方法]强化免疫1个月后采集静脉血，采用ELISA法测定麻疹IgG抗体，与未参加强化免疫者的抗体水平进行比较。同时，收集研究个体的麻疹免疫史、麻疹病史、麻疹密切接触史等流行病学资料。[结果]不能认为强化免疫对提高虹口区6～14岁学龄儿童麻疹抗体的阳性率、保护率及几何抗体平均浓度有影响，麻疹疫苗接种2剂次与接种3～4剂次相比，抗体水平差异无统计学意义。[结论]强化免疫的目的是消除免疫空白，在常规免疫高接种率的地区，实施大规模的强化免疫是对疫苗资源、人力资源和财力资源的浪费，而查漏补种是经济有效的预防措施。

关键词：学龄儿童麻疹疫苗强化免疫

Proliferation and apoptosis property of mesenchymal stem cells derived from peripheral blood under the culture conditions of hypoxia and serum deprivation

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Chinese Medical Journal 2011年第23期124卷 3959-3967页

作者： FU Wei-li JIA zhu-qing WANG Wei-ping ZHANG Ji-ying FU Xin DUAN Xiao-ning LEUNG Kevin Kar Ming ZHOU chun-yan YU Jia-kuo Institute of Sports Medicine Peking University Third Hospital Beijing 100191 China

Background The proliferation and apoptosis property of mesenchymal stem cells derived from peripheral blood （PB-MSCs） were investigated under hypoxia and serum deprivation conditions in vitro so as to evaluate the feasibility for autologous PB-MSCs applications in cartilage repair. Methods MSCs were mobilized into peripheral blood by granulocyte colony stimulating factor （G-CSF） and AMD3100. The blood samples were collected from central ear artery of rabbits. Adhered cells were obtained by erythrocyte lysis buffer and identified as MSCs by adherence to plastic, spindle shaped morphology, specific surface markers, differentiation abilities into osteoblasts, adipocytes and chondroblasts in vitro under appropriate conditions. MSCs were cultured in four groups at different oxygen tension （20% 02 and 2% O2）, with or without 10% fetal bovine serum （FBS） conditions： 20% 02 and 10% FBS complete medium （normal medium, N）, 20% 02 and serum deprivation medium （D）, 2% 02 and 10% FBS complete medium （hypoxia, H）, 2% 02 and serum deprivation （HD）. Cell proliferation was determined by CCK-8 assay. Apoptosis was detected by Annexin V/PI and terminal deoxynucleotide transferase dUTP nick end labeling （TUNEL） staining. Results Spindle-shaped adherent cells were effectively mobilized from peripheral blood by a combined administration of G-CSF plus AMD3100. These cells showed typical fibroblast-like phenotype similar to MSCs from bone marrow （BM-MSCs）, and expressed a high level of typical MSCs markers CD29 and CD44, but lacked in the expression of hematopoietic markers CD45 and major histocompatibility complex Class II （MHC II）. They could also differentiate into osteoblasts, adipocytes and chondroblasts in vitro under appropriate conditions. No significant morphological differences were found among the four groups. It was found that hypoxia could enhance proliferation of PB-MSCs regardless of serum concentration, but serum deprivation inhibited proliferation at the later stage of culture. Apart from that, hypoxia or serum deprivat

关键词： mesenchymal stem cells derived from peripheral blood hypoxia serum deprivation proliferation apoptosis

Identification of a serum three-microRNA signature for cervical cancer diagnosis

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Chinese Medical Journal 2021年第14期134卷 1756-1758页

作者： Min-Min Cao Qing-Xie Liu Xuan Zou Xing-Chen Fan Cheng Liu Shi-Yu Zhang Tong-Shan Wang chun-Yu Li Cheng Zhang Xiang-Nan Geng Ping Liu Wei zhu Department of Oncology First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Oncology Fudan University Shanghai Cancer CenterShanghai 200032China Department of Gastroenterology First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Intensive Care Unit First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Science and Technology First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Clinical Engineer First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China

To the Editor:Cervical cancer(CC)is one of the leading causes of cancer deaths in women around the world.[1]In clinical practice,screening methods such as human papillomavirus testing and cytology-based detection have the disadvantages of having a high false rate,being hysteretic or ineffective.[2]Novel non-invasive biomarkers that can help diagnose CC with higher specificity and sensitivity are in great demand.MicroRNA(miRNA)is a type of non-coding small RNA of 18 to 25 nucleotides in length.Circulating miRNAs show the great potential of being cancer biomarkers for the stable existence in peripheral serum or plasma.[3]In this study,we performed a four-stage experiment using a total of 108 CC and 108 normal control(NC)serum samples to explore the diagnostic potential of serum miRNAs in CC.The schematic diagram of the experimental procedure was shown in Supplementary Figure 1,http://***/CM9/A429.

关键词： specificity Figure false

Research progress and strategies for multifunctional rapeseed： A case study of China

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Journal of Integrative Agriculture 2016年第8期15卷 1673-1684页

作者： FU Dong-hui JIANG Ling-yan Annaliese S Mason XIAO Mei-li zhu Long-rong LI Li-zhi ZHOU Qing-hong SHEN Chang-jian HUANG chun-hui Key Laboratory of Crop Physiology Ecology and Genetic Breeding Ministry of Education/Agronomy College Jiangxi Agricultural University Nanchang 330045 P.R.China Department of Biochemistry University of Missouri Columbia 65211 USA Department of Plant Breeding IFZ Research Centre for Biosystems Land Use and Nutrition Justus Liebig University Heinrich- Buff-Ring 26-32 Giessen 35392 Germany Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development Jiangxi Agricultural University Nanchang 330045 P.R. China Linli County Rural Practical Technology Research Institute Changde 415000 P.R. China

financially supported by the Key R＆D Program of Jiangxi Province of China （20152ACF60010）;the Key Projects of Hunan Science and Technology Support Program of China （Application research of Guiye A male sterile line）;the Research Project of Humanities and Social Sciences in Universities of Jiangxi Province, China（JC1315）;supported by Emmy Noether DFG grant MA 6473/1-1

关键词： multifunctional rapeseed sightseeing rapeseed remediation of cadmium pollution rapeseed oil fodder vegetables

Immunohistochemical　study　of　gastrin　in　colorectal　carcinoma　tissues　and　its　adjacent　mucosa

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World Journal of Gastroenterology 1997年第2期3卷 27-29页

作者： JIANG chun Ping, CHEN Yu Quan, zhu Jian Wei, SHEN Hong Xun and YU Xiu

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关键词： colorectal neoplasms gastrin immunohistochemistry microscopy, electron

The i nvest i gat i on on stra i n of S i Ge/S i heteroep i tax i al system during high temperature annealing by RBS/Channeling

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The i nvest i gat i on on stra i n of S i Ge/S i heteroep i ...

’2001全国荷电粒子源、粒子束学术会议

作者： CHEN Chang-chun 1, zhu De-Zhang 1, ZHANG Shi-Li 2, LIU Hua-Ming 1, CAO Jian-Qin 1, ZHANG Zhi-Bin 1, PAN Hao-Chuang 1 and WANG Yong-Qi 11. Laboratory of Nuclear Analyses and Techniques, Shanghai institute of nuclear research, P. 0. BOX 800-204, Chinese Academy of Sciences, Shanghai 201800, China2. Kungliga Tekniska Hogskolan, Department of Electronics, Electrum 229, SE-16440 Kista, Sweden

The influence of the high temperature processing on strain stored in SiGehetero-epilayer was studied by means of RBS/Channeling.Channeling angular scanalong the axial direction in the(100) plane

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兔环状软骨损伤后软骨修复的实验研究

临床耳鼻咽喉科杂志 2000年第12期14卷 558-560页

作者：贾德静李贵泽朱春生武警总医院耳鼻咽喉科第四军医大学唐都医院耳鼻咽喉科西安710038

目的 :探讨甲状软骨在环状软骨缺损修复中的作用。方法 :将兔环状软骨前 1/ 3切除 ,分别利用游离甲状软骨修复 (游离组 )和带蒂甲状软骨修复 (带蒂组 ) ,观察软骨的病理变化。结果 :1游离软骨在 2个月时坏死吸收 ,为瘢痕组织所取代。 2... 详细信息

目的 :探讨甲状软骨在环状软骨缺损修复中的作用。方法 :将兔环状软骨前 1/ 3切除 ,分别利用游离甲状软骨修复 (游离组 )和带蒂甲状软骨修复 (带蒂组 ) ,观察软骨的病理变化。结果 :1游离软骨在 2个月时坏死吸收 ,为瘢痕组织所取代。 2带蒂软骨未见吸收 ,与环状软骨断端融合在一起。 3环状软骨的发育未受影响。4气道通气不受影响。结论 :游离软骨及带蒂软骨均能有效地修复环状软骨缺损。

关键词：环状软骨缺损甲状软骨移植

Assessing the association of appropriateness of coronary revascularization and 1-year clinical outcomes for patients with stable coronary artery disease in China

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Chinese Medical Journal 2020年第1期133卷 1-8页

作者： Shen Lin Heng Zhang Chen-Fei Rao Si-Peng Chen Shu-Bin Qiao Hong-Bing Yan Ke-Fei Dou Yong-Jian Wu Yi-Da Tang Xin-chun Yang zhu-Jun Shen Jian Liu Zhe Zheng National Clinical Research Center of Cardiovascular Diseases State Key Laboratory of Cardiovascular DiseaseFuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China Department of Cardiovascular Surgery Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China Department of Information Center Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China Department of Cardiology Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China Department of Cardiology Beijing Chao-Yang HospitalCapital Medical UniversityBeijing 100020China Department of Cardiology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Department of Cardiology Peking University People's HospitalBeijing 100044China.

Background:The Chinese appropriate use criteria(AUC)for coronary revascularization was released in 2016 to improve the use of coronary revascularization.This study aimed to evaluate the association between the appropriateness of coronary revascularization based on the Chinese AUC and 1-year outcomes in stable coronary artery disease(CAD)patients.Methods:We conducted a prospective,multi-center cohort study of stable CAD patients with coronary lesion stenosis≥50%.After the classification of appropriateness based on Chinese AUC,patients were categorized into the coronary revascularization group or the medical therapy group based on treatment received.The primary outcome was a composite of death,myocardial infarction,stroke,repeated revascularization,and ischemic symptoms with hospital admission.Results:From August 2016 to August 2017,6085 patients were consecutively enrolled.Coronary revascularization was associated with a lower adjusted hazard of 1-year major adverse cardiovascular and cerebrovascular events(MACCEs;hazard ratio[HR]:0.62;95%confidence interval[CI]:0.45-0.86;P=0.004)than medical therapy in patients with appropriate indications(n=1617).No significant benefit in 1-year MACCEs was found after revascularization compared to after medical therapy in patients with uncertain indications(n=2658,HR:0.81;95%CI:0.52-1.25;P=0.338)and inappropriate indications(n=1810,HR:0.80;95%CI:0.51-1.23;P=0.308).Conclusions:In patients with appropriate indications according to Chinese AUC,coronary revascularization was associated with significantly lower risk of MACCEs at 1 year.No benefit was found in coronary revascularization in patients with inappropriate indications.Our findings provide evidence for using Chinese AUC to guide clinical decision-making.

关键词： Stable coronary artery disease Appropriate use criteria Coronary revascularization

低强度牵张应力对异丙肾上腺素诱导下牙周膜成纤维细胞炎症反应的影响

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上海口腔医学 2022年第3期31卷 225-231页

作者：王雪纯吴艳棋梅鹏张博钧朱敏上海交通大学医学院附属第九人民医院口腔颅颌面科上海交通大学口腔医学院国家口腔医学中心国家口腔疾病临床医学研究中心上海市口腔医学重点实验室上海200011

目的 :探讨低强度牵张力对异丙肾上腺素(isoproterenol,ISO)诱导下人牙周膜成纤维细胞炎症反应的影响及其分子机制。方法:体外培养人牙周膜成纤维细胞(periodontal ligaments cells,PDLCs),给予一定浓度(0.01、0.1、1μmol/L)ISO刺激24... 详细信息

目的 :探讨低强度牵张力对异丙肾上腺素(isoproterenol,ISO)诱导下人牙周膜成纤维细胞炎症反应的影响及其分子机制。方法:体外培养人牙周膜成纤维细胞(periodontal ligaments cells,PDLCs),给予一定浓度(0.01、0.1、1μmol/L)ISO刺激24 h,设置空白对照组,同时施加不同强度(5%、10%、15%形变量)的牵张力,利用实时荧光定量PCR(RTqPCR)检测细胞内IL-1β、IL-6 mRNA的表达。设置空白対照组、ISO刺激组(0.1μmol/L)、低强度牵张力组(5%形变量)和ISO+低强度牵张力组,利用蛋白免疫印迹法(Western blot)检测内质网应激相关p-PERK、PERK、p-eIF2α、eIF2α、ATF4蛋白表达量的变化。应用细胞转染技术敲低PDLCs中PERK基因的表达,RT-qPCR检测低表达PERK的牙周膜成纤维细胞在ISO及5%形变量牵张力作用下IL-1β及IL-6 mRNA的表达。采用SPSS 22.0软件包对数据进行统计学分析。结果:ISO诱导可显著上调牙周膜成纤维细胞中IL-1β及IL-6 mRNA的表达(P<0.05);与对照组相比,5%形变量牵张力抑制ISO诱导下PDLCs中IL-1β和IL-6 mRNA的表达,差异有统计学意义(P<0.05);Western印迹结果显示,5%形变量牵张力可抑制ISO诱导引起的PERK、eIF2α磷酸化及ATF4的表达(P<0.05)。与阴性对照组相比,ISO诱导下PERK沉默的PDLCs中IL-1β及IL-6 mRNA表达显著降低(P<0.05)。结论:5%形变量牵张力可能通过内质网应激PERK通路抑制ISO诱导下牙周膜成纤维细胞的炎症反应。

关键词：牙周膜成纤维细胞牵张力内质网应激异丙肾上腺素炎症反应