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Altered profiles of nuclear matrix proteins during the differentiation of human gastric mucous adenocarcinoma MGc80-3 cells

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World Journal of Gastroenterology 2005年第30期11卷 4628-4633页

作者： chun-hong zhao Qi-Fu Li Laboratory of Cell Biology the Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering School of Life Sciences Xiamen University Xiamen 361005 Fujian Province China

AIM： To find and identify specific nuclear matrix proteins associated with proliferation and differentiation of carcinoma cells, which will be potential markers for cancer diagnosis and targets in cancer therapy. METHODS： Nuclear matrix proteins were selectively extracted from MGcS0-3 cells treated with or without hexamethylamine bisacetamide （HMBA）, and subjected to 2-D gel electrophoresis. The resulted protein patterns were analyzed by Melanie software. Spots of nuclear matrix proteins differentially expressed were excised and subjected to in situ digestion with trypsin. Peptide masses were obtained by matrix-assisted laser-desorption/ ionization time of flight mass spectrometry （MALDI-TOFMS） analysis and submitted for database searching using Mascot tool. RESULTS： The MGc80-3 cells were induced into differentiation by HMBA. There were 22 protein spots which changed remarkably in the nuclear matrix, from differentiation of MGcS0-3 cells compared to control. Eleven of which were identified. Seven proteinsactin, prohibitin, porin 31HL, heterogeneous nuclear dbonucleoprotein A2/B1, vimentin, ATP synthase, and heat shock protein 60 were downregulated, whereas three proteins - heat shock protein gp96, heat shock protein 90-beta, and valosin-containing protein were upregulated, and the oxygen-regulated protein was only found in the differentiated MGc80-3 cells. CONCLUSION： The induced differentiation of carcinoma cells is accompanied by the changes of nuclear matrix proteins. Further characterization of those proteins will show the mechanism of cellular proliferation and differentiation, as well as cancer differentiation.

关键词： Nuclear matrix proteins Cell differentiation Human gastric mucous adenocarcinoma MGc80-3 Hexamethylamine bisacetamide

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Synthesis and Anti-HIV Activity of Trisubstituted (3′R, 4′R)-3′,4′- Di-O-(S)-camphanoyl-(+)-cis-khellactone (DCK) Analogs

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Chinese Chemical Letters 2005年第10期16卷 1297-1300页

作者： chun hong zhao Ting ZHOU Lan XIE Jing Yun LI Zuo Yi BAO zhao Wen LOU Kuo Hsiung LEE Beijing Institute of Pharmacology ＆ Toxicology Beijing 100850 Department of Chemistry Hubei University Wuhan Hubei 430062 Beijing Institute of Microbiology ＆ Epidemiology Beijing 100850 School of Pharmacy University of North Carolina at Chapel HillNC 27599 USA

To further explore the potential of DCK analogs as anti-HIV drug candidates, ten new tri-substituted （3＇R,4＇R）-3＇,4＇-di-O-（S）-camphanoyl-（＋）-cis-khellactone （DCK） derivatives （4-13） were designed, synth... 详细信息

关键词： anti-HIV agent DCK analogs synthesis.

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Changes of Nuclear Matrix Proteins Following the Differentiation of Human Osteosarcoma MG-63 Cells

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Genomics, Proteomics & Bioinformatics 2006年第1期4卷 10-17页

作者： chun-hong zhao Qi-Fu Li Yan zhao Jing-Wen Niu Zhi-Xing Li Jin-An Chen Laboratory of Cell Biology the Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering Analysis and Testing Center School of Life Sciences Xiamen University Xiamen 361005 China

Human osteosarcoma MG-63 cells were induced into differentiation by 5 mmol/L hexamethylene bisacetamide （HMBA）. Their nuclear matrix proteins （NMPs） were selectively extracted and subjected to two-dimensional gel electrophoresis analysis. The results of protein patterns were analyzed by Melanie software. The spots of differentially expressed NMPs were excised and subjected to in situ digestion with trypsin. The maps of peptide mass fingerprinting were obtained by MALDI-TOF-MS analysis, and were submitted for NCBI database searches by Mascot tool. There were twelve spots changed remarkably during the differentiation induced by HMBA, nine of which were identified. The roles of the regulated proteins during the MG-63 differentiation were analyzed. This study suggests that the induced differentiation of cancer cells is accompanied by the changes of NMPs, and confirms the presence of some specific NMPs related to the cancer cell proliferation and differentiation. The changed NMPs are potential markers for cancer diagnosis or targets for cancer therapy.

关键词： nuclear matrix proteins cell differentiation human osteosarcoma HMBA

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Adsorption and Desorption of Ammonium in Wetland Soils Subject to Freeze-Thaw Cycles

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Pedosphere 2011年第2期21卷 251-258页

作者： YU Xiao-Fei ZHANG Yu-Xia ZOU Yuan-chun zhao hong-Mei LU Xian-Guo WANG Guo-Ping Northeast Institute of Geography and Agroecology Chinese Academy of Sciences Changehun 130012 (China) Graduate University of Chinese Academy of Sciences Beijing 100049 (China)

Nitrogen （N） cycling in boreal peatland ecosystems may be influenced in important ways by freeze-thaw cycles （FTCs）. Adsorption and desorption of ammonium ions （NH4＋） were examined in a controlled laboratory experiment for soils sampled from palustrine wetland, riverine wetland, and farmland reclaimed from natural wetland in response to the number of FTCs. The results indicate that freeze-thaw significantly increased the adsorption capacity of NH： and reduced the desorption potential of NH4＋ in the wetland soils. There were significant differences in the NH4＋ adsorption amount between the soils with and without freeze-thaw treatment. The adsorption amount of NH4＋ increased with increasing FTCs. The palustrine wetland soil had a greater adsorption capacity and a weaker desorption potential of NH4＋ than the riverine wetland soil because of the significantly higher clay content and cation exchange capacity （CEC） of the riverine wetland soil. Because of the altered soil physical and chemical properties and hydroperiods, the adsorption capacity of NH4＋ was smaller in the farmland soil than in the wetland soils, while the desorption potential of the farmland soil was higher than that of the wetland soils. Thus, wetland reclamation would decrease adsorption capacity and increase desorption potential of NH4＋, which could result in N loss from the farmland soil. FTCs might mitigate N loss from soils and reduce the risk of water pollution in downstream ecosystems.

关键词： cation exchange capacity clay content farmland hydroperiod N loss

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Prediction of fatal adverse prognosis in patients with fever-related diseases based on machine learning:a retrospective study

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Chinese Medical Journal 2020年第5期133卷 583-589页

作者： chun-hong zhao Hui-Tao Wu He-Bin Che Ya-Nan Song Yu-Zhuo zhao Kai-Yuan Li hong-Ju Xiao Yong-Zhi Zhai Xin Liu hong-Xi Lu Tan-Shi Li Medical School of Chinese People’s Liberation Army Beijing 100853China Department of Emergency The First Medical Center of Chinese People’s Liberation Army General HospitalBeijing 100853China National Engineering Laboratory for Medical Big Data Application Technology The First Medical Center to Chinese People’s Liberation Army General HospitalBeijing 100853China

Background:Fever is the most common chief complaint of emergency *** identification of patients at an increasing risk of death may avert adverse *** aim of this study was to establish an early prediction model of fatal adverse prognosis of fever patients by extracting key indicators using big data ***:A retrospective study of patients’data was conducted using the Emergency Rescue Database of Chinese People’s Liberation Army General *** were divided into the fatal adverse prognosis group and the good prognosis *** commonly used clinical indicators were *** feature elimination method was used to determine the optimal number of the included *** the training model,logistic regression,random forest,adaboost,and bagging were *** also collected the emergency room data from December 2018 to December 2019 with the same inclusion and exclusion *** performance of the model was evaluated by accuracy,F1-score,precision,sensitivity,and the areas under receiver operator characteristic curves(ROC-AUC).Results:The accuracy of logistic regression,decision tree,adaboost and bagging was 0.951,0.928,0.924,and 0.924,F1-scores were 0.938,0.933,0.930,and 0.930,the precision was 0.943,0.938,0.937,and 0.937,ROC-AUC were 0.808,0.738,0.736,and 0.885,***-AUC of ten-fold cross-validation in logistic and bagging models were 0.80 and 0.87,*** top six coefficients and odds ratio(OR)values of the variables in the logistic regression were cardiac troponin T(CTnT)(coefficient=0.346,OR=1.413),temperature(T)(coefficient=0.235,OR=1.265),respiratory rate(RR)(coefficient=–0.206,OR=0.814),serum kalium(K)(coefficient=0.137,OR=1.146),pulse oxygen saturation(SPO2)(coefficient=–0.101,OR=0.904),and albumin(ALB)(coefficient=–0.043,OR=0.958).The weights of the top six variables in the bagging model were:CTnT,RR,lactate dehydrogenase,serum amylase,heart rate,and systolic blood ***

关键词： Fever Infection Machine learning Prognosis

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The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson's disease

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Neural Regeneration Research 2025年第8期20卷 2361-2372页

作者： Shengyang Zhou Ting Li Wei Zhang Jian Wu Hui hong Wei Quan Xinyu Qiao chun Cui Chenmeng Qiao Weijiang zhao Yanqin Shen Laboratory of Neurodegenerative and Neuroinjury Diseases Wuxi Medicine SchoolJiangnan UniversityWuxiJiangsu ProvinceChina

Interferon regulatory factor 7 plays a crucial role in the innate immune ***,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains *** we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial *** the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 *** addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and *** together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.

关键词： cyclic guanosine monophosphate adenosine monophosphate synthase H151 interferon regulatory factor 7 M1 phenotype neurodegenerative disease neuroinflammation Parkinson’s disease RU521 STING type I interferon

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Constructing Donor–Acceptor‑Linked COFs Electrolytes to Regulate Electron Density and Accelerate the Li^(+)Migration in Quasi‑Solid‑State Battery

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Nano-Micro Letters 2025年第1期17卷 456-471页

作者： Genfu zhao Hang Ma Conghui Zhang Yongxin Yang Shuyuan Yu Haiye Zhu Yongjiang Sun hong Guo School of Materials and Energy International Joint Research Center for Advanced Energy Materials of Yunnan ProvinceYunnan UniversityKunming 650091People’s Republic of China Yunnan Yuntianhua Co. LtdR&D CenterKunming 650228People’s Republic of China Southwest United Graduate School Kunming 650091People’s Republic of China

Regulation the electronic density of solid-state electrolyte by donor–acceptor(D–A)system can achieve highly-selective Li^(+)transportation and conduction in solid-state Li metal *** study reports a high-performance solid-state electrolyte thorough D–A-linked covalent organic frameworks(COFs)based on intramolecular charge transfer *** other reported COFbased solid-state electrolyte,the developed concept with D–A-linked COFs not only achieves electronic modulation to promote highly-selective Li^(+)migration and inhibit Li dendrite,but also offers a crucial opportunity to understand the role of electronic density in solid-state Li metal *** introduced strong electronegativity F-based ligand in COF electrolyte results in highlyselective Li^(+)(transference number 0.83),high ionic conductivity(6.7×10^(-4)S cm^(−1)),excellent cyclic ability(1000 h)in Li metal symmetric cell and high-capacity retention in Li/LiFePO_(4)cell(90.8%for 300 cycles at 5C)than substituted C-and N-based *** is ascribed to outstanding D–A interaction between donor porphyrin and acceptor F atoms,which effectively expedites electron transferring from porphyrin to F-based ligand and enhances Li^(+)***,we anticipate that this work creates insight into the strategy for accelerating Li^(+)conduction in high-performance solid-state Li metal batteries through D–A system.

关键词： Electronic modulation engineering Donor-acceptor-linked covalent organic frameworks Quasi-solid-state Li metal battery

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Inhibiting SHP2 reduces glycolysis, promotes microglial M1 polarization, and alleviates secondary inflammation following spinal cord injury in a mouse model

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Neural Regeneration Research 2025年第3期20卷 858-872页

作者： Xintian Ding chun Chen Heng zhao Bin Dai Lei Ye Tao Song Shuai Huang Jia Wang Tao You Department of Orthopedics The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Orthopedics Provincial Hospital Affiliated to Anhui Medical UniversityHefeiAnhui ProvinceChina

Reducing the secondary inflammatory response, which is partly mediated by microglia, is a key focus in the treatment of spinal cord injury. Src homology 2-containing protein tyrosine phosphatase 2(SHP2), encoded by PTPN11, is widely expressed in the human body and plays a role in inflammation through various mechanisms. Therefore, SHP2 is considered a potential target for the treatment of inflammation-related diseases. However, its role in secondary inflammation after spinal cord injury remains unclear. In this study, SHP2 was found to be abundantly expressed in microglia at the site of spinal cord injury. Inhibition of SHP2 expression using siRNA and SHP2 inhibitors attenuated the microglial inflammatory response in an in vitro lipopolysaccharide-induced model of inflammation. Notably, after treatment with SHP2 inhibitors, mice with spinal cord injury exhibited significantly improved hind limb locomotor function and reduced residual urine volume in the bladder. Subsequent in vitro experiments showed that, in microglia stimulated with lipopolysaccharide, inhibiting SHP2 expression promoted M2 polarization and inhibited M1 polarization. Finally, a co-culture experiment was conducted to assess the effect of microglia treated with SHP2 inhibitors on neuronal cells. The results demonstrated that inflammatory factors produced by microglia promoted neuronal apoptosis, while inhibiting SHP2 expression mitigated these effects. Collectively, our findings suggest that SHP2 enhances secondary inflammation and neuronal damage subsequent to spinal cord injury by modulating microglial phenotype. Therefore, inhibiting SHP2 alleviates the inflammatory response in mice with spinal cord injury and promotes functional recovery postinjury.

关键词： apoptosis glycolysis inflammatory response microglia neurons polarization spinal cord injury Src homology 2-containing protein tyrosine phosphatase 2

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Deciphering Water Oxidation Catalysts:The Dominant Role of Surface Chemistry over Reconstruction Degree in Activity Promotion

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Nano-Micro Letters 2025年第3期17卷 340-354页

作者： Li An Jianyi Li Yuanmiao Sun Jiamin Zhu Justin Zhu Yeow Seow hong Zhang Nan Zhang Pinxian Xi * Zhichuan JXu chun‑Hua Yan State Key Laboratory of Applied Organic Chemistry Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu ProvinceFrontiers Science Center for Rare IsotopesCollege of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000People’s Republic of China School of Materials Science and Engineering Nanyang Technological UniversitySingapore 639798Singapore Faculty of Materials Science and Energy Engineering Institute of Technology for Carbon NeutralityShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055People’s Republic of China Key Laboratory of Electromagnetic Materials and Devices National Center for International Research On Photoelectric and Energy MaterialsSchool of Materials and EnergyElectron Microscopy CenterYunnan UniversityKunming 650091People’s Republic of China State Key Laboratory of Baiyunobo Rare Earth Resource Researches and Comprehensive Utilization Baotou Research Institute of Rare EarthsBaotou 014030People’s Republic of China Beijing National Laboratory for Molecular Sciences State Key Laboratory of Rare Earth Materials Chemistry and ApplicationsPKU‑HKU Joint Laboratory in Rare Earth Materials and Bioinorganic ChemistryPeking UniversityBeijing 100871People’s Republic of China

Water splitting hinges crucially on the availability of electrocatalysts for the oxygen evolution *** surface reconstruction has been widely observed in perovskite catalysts,and the reconstruction degree has been often correlated with the activity ***,a systematic study on the roles of Fe substitution in activation of perovskite LaNiO_(3)is *** substituting Fe content influences both current change tendency and surface reconstruction ***_(0.9)Fe_(0.1)O_(3)is found exhibiting a volcano-peak intrinsic activity in both pristine and reconstructed among all substituted perovskites in the LaNi_(1-x)Fe_(x)O_(3)(x=0.00,0.10,0.25,0.50,0.75,1.00)*** reconstructed LaNi_(0.9)Fe_(0.1)O_(3)shows a higher intrinsic activity than most reported NiFe-based ***,density functional theory calculations reveal that Fe substitution can lower the O 2p level,which thus stabilize lattice oxygen in LaNi0.9Fe0.1O3 and ensure its long-term ***,it is vital interesting that activity of the reconstructed catalysts relied more on the surface chemistry rather than the reconstruction *** effect of Fe on the degree of surface reconstruction of the perovskite is decoupled from that on its activity enhancement after surface *** finding showcases the importance to customize the surface chemistry of reconstructed catalysts for water oxidation.

关键词： Oxygen evolution reaction Perovskite oxides Doping Activation and reconstruction

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Regulation and function of endoplasmic reticulum autophagy in neurodegenerative diseases

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Neural Regeneration Research 2025年第1期20卷 6-20页

作者： Xiu-Yun zhao De-En Xu Ming-Lei Wu Ji-Chuan Liu Zi-Ling Shi Quan-hong Ma Department of Neurology and Clinical Research Center of Neurological Disease the Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Neuroscience&Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases Soochow UniversitySuzhouJiangsu ProvinceChina Department of Neurology Jiangnan University Medical CenterWuxiJiangsu ProvinceChina

The endoplasmic reticulum,a key cellular organelle,regulates a wide variety of cellular *** reticulum autophagy,one of the quality control systems of the endoplasmic reticulum,plays a pivotal role in maintaining endoplasmic reticulum homeostasis by controlling endoplasmic reticulum turnover,remodeling,and *** this review,we briefly describe the endoplasmic reticulum quality control system,and subsequently focus on the role of endoplasmic reticulum autophagy,emphasizing the spatial and temporal mechanisms underlying the regulation of endoplasmic reticulum autophagy according to cellular *** also summarize the evidence relating to how defective or abnormal endoplasmic reticulum autophagy contributes to the pathogenesis of neurodegenerative *** summary,this review highlights the mechanisms associated with the regulation of endoplasmic reticulum autophagy and how they influence the pathophysiology of degenerative nerve *** review would help researchers to understand the roles and regulatory mechanisms of endoplasmic reticulum-phagy in neurodegenerative disorders.

关键词： autophagy endoplasmic reticulum endoplasmic reticulum autophagy endoplasmic reticulum quality control system endoplasmic reticulum receptors endoplasmic reticulum-associated degradation neurodegeneration neurodegenerative disease selective autophagy unfolded protein response

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