检索结果-南通市图书馆

Alpha-fetoprotein-targeted reporter gene expression imaging in hepatocellular carcinoma

World Journal of Gastroenterology 2016年第27期22卷 6127-6134页

作者： Kwang Il Kim Hye Kyung Chung Ju Hui Park Yong Jin Lee joo hyun Kang Molecular Imaging Research Center Korea Institute of Radiological and Medical Sciences Korea Drug Development Platform using Radio-Isotope Korea Institute of Radiological and Medical Sciences

Hepatocellular carcinoma (HCC) is one of the most common cancers in Eastern Asia, and its incidence is increasing globally. Numerous experimental models have been developed to better our understanding of the pathogenic mechanism of HCC and to evaluate novel therapeutic approaches. Molecular imaging is a convenient and up-to-date biomedical tool that enables the visualization, characterization and quantification of biologic processes in a living subject. Molecular imaging based on reporter gene expression, in particular, can elucidate tumor-specific events or processes by acquiring images of a reporter gene’s expression driven by tumor-specific enhancers/promoters. In this review, we discuss the advantages and disadvantages of various experimental HCC mouse models and we present in vivo images of tumor-specific reporter gene expression driven by an alpha-fetoprotein (AFP) enhancer/promoter system in a mouse model of HCC. The current mouse models of HCC development are established by xenograft, carcinogen induction and genetic engineering, representing the spectrum of tumor-inducing factors and tumor locations. The imaging analysis approach of reporter genes driven by AFP enhancer/promoter is presented for these different HCC mouse models. Such molecular imaging can provide longitudinal information about carcinogenesis and tumor progression. We expect that clinical application of AFP-targeted reporter gene expression imaging systems will be useful for the detection of AFP-expressing HCC tumors and screening of increased/decreased AFP levels due to disease or drug treatment.

关键词： Alpha-fetoprotein Hepatocellular carcinoma Molecular imaging Reporter gene Tumor-specific enhancer/promoter

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of electrical stimulation on neural regeneration via the p38-RhoA and ERK1/2-Bcl-2 pathways in spinal cord-injured rats

引用

Neural Regeneration Research 2018年第2期13卷 340-346页

作者： Min Cheol joo chul Hwan Jang Jong Tae Park Seung Won Choi Seungil Ro Min Seob Kim Moon Young Lee Department of Rehabilitation Medicine and Institute of Wonkwang Medical Science School of Medicine Wonkwang University Department of Neurosurgery School of Medicine Wonkwang University Department of Anesthesiology and Pain Medicine School of Medicine Wonkwang University Department of Physiology and Cell Biology School of Medicine University of Nevada Department of Physiology and Institute of Wonkwang Medical Science School of Medicine Wonkwang University

Although electrical stimulation is therapeutically applied for neural regeneration in patients, it remains unclear how electrical stimulation exerts its effects at the molecular level on spinal cord injury （SCI）. To identify the signaling pathway involved in electrical stimulation improving the function of injured spinal cord, 21 female Sprague-Dawley rats were randomly assigned to three groups： control （no surgical intervention, n = 6）, SCI （SCI only, n = 5）, and electrical simulation （ES; SCI induction followed by ES treatment, n = 10）. A complete spinal cord transection was performed at the 10^th thoracic level. Electrical stimulation of the injured spinal cord region was applied for 4 hours per day for 7 days. On days 2 and 7 post SCI, the Touch-Test Sensory Evaluators and the Basso-Beattie-Bresnahan locomotor scale were used to evaluate rat sensory and motor function. Somatosensory-evoked potentials of the tibial nerve of a hind paw of the rat were measured to evaluate the electrophysiological function of injured spinal cord. Western blot analysis was performed to measure p38-RhoA and ERK1/2-Bcl-2 pathways related protein levels in the injured spinal cord. Rat sensory and motor functions were similar between SCI and ES groups. Com- pared with the SCI group, in the ES group, the latencies of the somatosensory-evoked potential of the tibial nerve of rats were significantly shortened, the amplitudes were significantly increased, RhoA protein level was significantly decreased, protein gene product 9.5 expression, ERK1/2, p38, and Bcl-2 protein levels in the spinal cord were significantly increased. These data suggest that ES can promote the recovery of electrophysiological function of the injured spinal cord through regulating p38-RhoA and ERK1/2-Bcl-2 pathway-related protein levels in the injured spinal cord.

关键词： Bcl-2 ERKI/2 p38 PGP9.5 RhoA spinal cord injury somatosensory evoked potential muscle contraction electrical impulses neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Extensive complex thoracoabdominal aortic aneurysm salvaged by surgical graft providing landing zone for endovascular graft:A case report

引用

World Journal of Clinical Cases 2022年第15期10卷 5005-5011页

作者： Albert Youngwoo Jang Pyung Chun Oh Jin Mo Kang chul hyun Park Woong Chol Kang Department of Cardiology Gachon University Gil Medical CenterIncheon 1198South Korea Department of Internal Medicine Gil Medical CenterGachon Cardiovascular Research InstituteGachon University College of MedicineIncheon 1198South Korea Division of Vascular Surgery Gachon University Gil Medical CenterIncheon 1198South Korea Department of Thoracic and Cardiovascular Surgery Gachon University Gil Medical CenterIncheon 1198South Korea Division of Cardiology Gil Medical CenterGachon University of Medicine and ScienceIncheon 1198South Korea

BACKGROUND Surgical repair of complex abdominal aortic aneurysm is associated with a higher perioperative mortality and *** advent of endovascular aortic repair(EVAR)has reduced perioperative complications,although the utilization of such techniques is limited by lesion characteristics,such as involvement of the visceral or renal arteries(RA)and/or presence of a sealing *** SUMMARY A 60-year-old male presented with a Crawford type IV complex thoracoabdominal aortic aneurysm(CAAA)starting directly distal to the diaphragm extending to both common iliac arteries(CIAs).The CAAA consist of a proximal and distal aneurysmal sac separated by a 1 cm-healthy zone in the infrarenal *** to the poor performance of the patient and the expansive disease,we planned a stepwise-combined surgery and EVAR to minimize invasiveness.A branched graft was implanted after surgical debranching of the visceral and *** the patient had renal and liver injury after surgery,the second stage EVAR was performed 10 mo *** stent graft was implanted from the distal portion of surgical branched graft to both CIAs during *** patient has been uneventful for 5-years after discharge and is being followed in the outpatient *** The current case demonstrates that the surgical graft can provide a landing zone for second stage EVAR to avoid aggressive surgery in patients with poor performance with a long hostile CAAA.

关键词： Complex thoracoabdominal aortic aneurysm Abdominal aortic aneurysm surgery Endovascular aortic repair Common iliac artery aneurysm Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Development of real-time onion disease monitoring system using image acquisition

引用

Frontiers of Agricultural Science and Engineering 2018年第4期5卷 469-474页

作者： Du-Han KIM Kyeong-Hwan LEE Chang-hyun CHOI Tae-hyun CHOI Yong-joo KIM Department of Biosystems Machinery Engineering Chung-Nam National UniversityDaejeon 305-764Republic of Korea Department of Rural and Biosystems Engineering Chonnam National UniversityGwangju 500-757Republic of Korea Department of Bio-Mechatronic Engineering Sungkyunkwan UniversitySuwonGyeonggi 440-746Republic of Korea Sensoreye R&D Solutions Daejeon 302-861Republic of Korea

In this study, real-time disease monitoring was conducted on onion which is the most representative crop in Republic of Korea, using an image acquisition system newly developed for the mobile measurement of phenotype. The purpose of this study was to improve the accuracy of prediction of disease and state variables by processing images acquired from monitoring. The image acquisition system was consisted of two parts, a motorized driving system and a PTZ(pan, tilt and zoom) camera to take images of the plants. The acquired images were processed as follows. Noise was removed through an image filter and RGB(red, green and blue) colors were converted to HSV(hue, saturation and value), which enabled thresholding of areas with different colors and properties for image binarization by comparing the color of onion leaf with ambient areas. Four objects with the most significant browning in the onion leaf to the naked eye were selected as the samples for data acquired. The thresholding method with image processing was found to be superior to the naked eye in identifying accurate disease areas. In addition, it was found that the incidence of disease was different in each disease area ratio. As a result, the use of image acquisition system in image processing analysis will enable more prompt detection of any changes in the onion and monitoring of disease outbreaks during the crop lifecycle.

关键词： imaging acquisition system disease downy mildew onion

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Modulatory effects of the fruits of Tribulus terrestris L. on the function of atopic dermatitis-related calcium channels,Orai1 and TRPV3

引用

Asian Pacific Journal of Tropical Biomedicine 2016年第7期6卷 580-585页

作者： joo hyun Nam Hyo Won Jung Young-Won Chin Woo Kyung Kim Hyo Sang Bae Department of Physiology College of MedicineChannelopathy Research Center(CRC)Dongguk University Department of Herbology College of Korean MedicineDongguk University College of Pharmacy Dongguk University Department of Internal Medicine Graduate School of MedicineChannelopathy Research Center(CRC)Dongguk University Department of Sasang Constitutional Medicine College of Korean MedicineDongguk University

Objective: To examine the effects of Tribulus terrestris L.(T. terrestris) extract on the modulation of calcium channels to evaluate its use in topical agents for treatment of atopic ***: The 70% methanol extract of T. terrestris was prepared. Human HEK293 T cells with over-expressed calcium release-activated calcium channel protein 1(Orai1),transient receptor potential vanilloid 1, or transient receptor potential vanilloid 3(TRPV3)were treated with T. terrestris extract. Modulation of ion channels was measured using a conventional whole-cell patch-clamp ***: T. terrestris extract(100 mg/m L) significantly inhibited Orai1 activity in Orai1-stromal interaction molecule 1 co-overexpressed HEK293 T cells. In addition, T. terrestris extract significantly increased the TRPV3 activity compared with 2-Aminoethyl diphenylborinate(100 mmol/L), which induces the full activation of ***: Our results suggest that T. terrestris extract may have a therapeutic potential for recovery of abnormal skin barrier pathologies in atopic dermatitis through modulating the activities of calcium ion channels, Orai1 and TRPV3. This is the first study to report the modulatory effect of a medicinal plant on the function of ion channels in skin barrier.

关键词： Tribulus terrestris L. Atopic dermatitis Ion channels ORAI1 TRPV3

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Seroepidemiology of echovirus 30 in Korean children

引用

World Journal of Pediatrics 2017年第6期13卷 611-614页

作者： joo Young Lee Yumi Seo Ui Yoon Choi Jong-hyun Kim Jin Han Kang Department of Pediatrics College of MedicineThe Catholic University of KoreaSeoulRepublic of Korea

Background:Although aseptic meningitis associated with echovirus type 30 has emerged as a global public health concern,no data have been reported on children's immune status against echovirus type *** current study aimed to investigate the seropositivity among Korean children for antibodies against echovirus ***:Two hundred and fifty residual serum samples were collected at ***'s *** were categorized by age into four groups:group 1 (3 months-2 years),group 2 (3-6 years),group 3 (7-10 years) and group 4 (11-15 years).Neutralizing antibodies against echovirus 30 were ***:Seroprotective neutralizing antibodies against echovirus 30 were detected in 129 (49%) *** rates were 23%,48%,55% and 73% in groups 1-4,*** antibody titers,1:256-1:512 was the highest neutralizing antibody titer range in group 2,while 1:1024-1:2048 in group 3 and *** the seropositive individuals in group 3 and 4,6% and 12% had neutralizing antibody titers of 1:2048,***:The seropositivity rate increased significantly with age..The distribution of neutralizing antibody titers varied by age group,and higher ranges of neutralizing antibody titers were observed in higher age *** findings suggest high susceptibility to echovirus 30 infection in children younger than 2 years *** 30 infection in childhood may have contributed to increased neutralizing antibody titers with age.

关键词： echovirus 30 Korea seroepidemiology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of ethanol on the tonicity of corporal tissue and the intracellular Ca^2＋ concentration of human corporal smooth muscle cells

引用

Asian Journal of Andrology 2010年第6期12卷 890-898页

作者： Sung chul Kam Mee Ree Chae Ji Young Kim Seol Ho Choo Deok hyun Han Sung Won Lee Department of Urology Samsung Medical Center Sungkyunkwan University School of Medicine Seoul 135-710 Korea

Heavy alcohol consumption is associated with an increased risk of erectile dysfunction （ED）; however, the acute effects of ethanol （EtOH） on penile tissue are not fully understood. We sought to investigate the effects of EtOH on corporal tissue tonicity, as well as the intracellular Ca^2＋ concentration （[Ca^2＋]i） and potassium channel activity of corporal smooth muscle. Strips of corpus cavernosum （CC） from rabbits were mounted in organ baths for isometric tension studies. Electrical field stimulation （EFS） was applied to strips precontracted with 10 μmol L^-1 phenylephrine as a control. EtOH was then added to the organ bath and incubated before EFS. The [Ca^2＋]i levels were monitored by the ratio of fura-2 fluorescence intensities using the fura-2 loading method. Single-channel and whole-cell currents were recorded by the conventional patch-clamp technique in short-term cultured smooth muscle cells from human CC tissue. The corpus cavernosal relaxant response of EFS was decreased in proportion to the concentration of EtOH. EtOH induced a sustained increase in [Ca^2＋]i in a dose-dependent manner, Extracellular application of EtOH significantly increased whole-cell K^＋ currents in a concentration-dependent manner （P 〈 0.05）. EtOH also increased the open probability in cell-attached patches; however, in inside-out patches, the application of EtOH to the intracellular aspect of the patches induced slight inhibition of Ca^2＋-activated potassium channel （KCa） activity. EtOH caused a dose-dependent increase in cavemosal tension by alterations to [Ca^2＋]i. Although EtOH did not affect KCa channels directly, it increased the channel activity by increasing [Ca^2＋]i. The increased corpus cavemosal tone caused by EtOH might be one of the mechanisms of ED after heavy drinking.

关键词： alcohol Ca^2+-activated potassium channel corporal smooth muscle penile erection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Photonic Crystal Fiber Attenuator

引用

光学学报 2003年第S1期23卷 -页

作者： joo Beom Eom Hokyung Kim Jinchae Kim Un-chul Paek Byeong Ha Lee Department of Information and Communications Kwangju Institute of Science and Technology 1 Oryong-dong

We propose a novel fiber attenuator based on photonic crystal fibers. The difference in the modal field diameters of a conventional single mode fiber and a photonic crystal fiber was used. A variable optical attenuato... 详细信息

关键词： photonic crystal fiber variable optical attenuator single mode fiber

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High-frequency magnetic stimulation attenuates beta-amyloid protein 1-42 neurotoxicity in organotypic hippocampal slices

引用

Neural Regeneration Research 2010年第18期5卷 1365-1372页

作者： Don-Kyu Kim Young chul Yoon Soo Ahn Chae Kyung Mook Seo Tai Ryoon Han Si-hyun Kang Department of Physical Medicine and Rehabilitation Chung-Ang University College of Medicine Department of Neurology Chung-Ang University College of Medicine Department of Pediatrics Chung-Ang University College of Medicine Department of Physical Medicine and Rehabilitation Seoul National University

Repetitive transcranial magnetic stimulation （rTMS） has been utilized as a therapeutic tool for neurodegenerative disorders including Alzheimer＇s disease. However, the precise mechanisms of its clinical effects remain unknown. β-amyloid （Aβ） exhibits direct neurotoxic effects and is closely related to neuronal degeneration in Alzheimer＇s disease. Therefore, it has been hypothesized that the neuroprotective effects of rTMS are related to the mechanisms of protection against Aβ neurotoxicity. Organotypic hippocampal slices were prepared from 8-day old, Sprague Dawley rats. The tissue slices were exposed to 100 μmol/L Al3142 since day 12 in vitro with and without high-frequency （20 Hz） magnetic stimulation. Magnetic stimulation efficacy was evaluated by measuring neuronal nuclei （NeuN） protein expression and by observing cultures following propidium iodide fluorescence staining and bromodeoxyuridine （BrdU） immunohistochemistry. Lactate dehydrogenase activity was detected in the culture media to evaluate hippocampal neuronal damage. Our results demonstrated that high-frequency magnetic stimulation significantly reversed the reduction of NeuN protein expression because of Aβ1-42 exposure （P 〈 0.05） and significantly reduced the number of damaged cells in the hippocampal slices （P 〈 0.05）. However, lactate dehydrogenase levels and anti-BrdU staining results did not reveal any statistical differences These findings indicate that high-frequency magnetic stimulation might have protective effect on hippocampal neurons from Aβ1-42 neurotoxicity.

关键词： organotypic hippocampus amyloid beta-protein magnetic stimulation nerve degeneration/metabolism nerve degeneration/pathology organ culture techniques rats, Sprague Dawiey

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Favorable surgical treatment outcomes for chronic constipation with features of colonic pseudo-obstruction

引用

World Journal of Gastroenterology 2012年第32期18卷 4441-4446页

作者： Eon chul Han Heung-Kwon Oh Heon-Kyun Ha Eun Kyung Choe Sang Hui Moon Seung-Bum Ryoo Kyu joo Park Department of Surgery Seoul National University College of MedicineSeoul 110-744South Korea Seoul National University Hospital Gangnam Center Seoul 110-744South Korea

AIM:To determine long-term outcomes of surgical treatments for patients with constipation and features of colonic ***:Consecutive 42 patients who underwent surgery for chronic constipation within the last 13 years were prospectively *** identified a subgroup with colonic pseudo-obstruction(CPO) features,with dilatation of the colon proximal to the narrowed transitional zone,in contrast to typical slowtransit constipation(STC),without any dilated colonic *** outcomes of surgical treatments for chronic constipation with features of CPO were analyzed and compared with outcomes for ***:Of the 42 patients who underwent surgery for constipation,33 patients had CPO with dilatation of the colon proximal to the narrowed transitional *** were 16 males and 17 females with a mean age of 51.2 ± 16.1 *** had symptoms of chronic intestinal obstruction,including abdominal distension,pain,nausea,or vomiting,and the mean duration of symptoms was 67 mo(range:6-252 mo).Preoperative defecation frequency was 1.5 ± 0.6 times/wk(range:1-2 times/wk).Thirty-two patients underwent total colectomy,and one patient underwent diverting transverse *** was no surgery-related *** histologic examination showed hypoganglionosis or agangliosis in 23 patients and hypoganglionosis combined with visceral neuropathy or myopathy in 10 *** contrast,histology of STC group revealed intestinal neuronal dysplasia type B(n = 6) and visceral myopathy(n = 3).Early postoperative complications developed in six patients with CPO;wound infection(n = 3),paralytic ileus(n = 2),and intraabdominal abscess(n = 1).Defecation frequencies 3 mo after surgery improved to 4.2 ± 3.2 times/d(range:1-15 times/d).Long-term follow-up(median:39.7 mo) was available in 32 patients;all patients had improvements in constipation symptoms,but two patients needed intermittent medication for management of *** 32 patients had distinct

关键词： Constipation Total colectomy Pseudoobstruction Surgical outcome Hypoganglionosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：