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Migfilin promotes migration and invasion in glioma by driving EGFR and MMP-2 signalings:A positive feedback loop regulation

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Journal of Genetics and Genomics 2017年第12期44卷 557-565页

作者： Yunwei Ou Qingnan wu chuanyue wu Xuefeng Liu Yongmei Song Qimin Zhan Department of Neurosurgery Beijing Tiantan HospitalCapital Medical UniversityBeijing 100050China Beijing Neurosurgical Institute Capital Medical UniversityBeijing 100050China State Key Laboratory of Molecular Oncology National Cancer Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of Pathology University of PittsburghPittsburgh 15261USA Department of Biology and Shenzhen Key Laboratory of Cell Microenvironment South University of Science and Technology of ChinaShenzhen 518055China china National Clinical Research Center for Neurological Diseases BeUing 100050China

Glioma is the most common type of primary brain tumors in the central nervous system（CNS）. Migfilin occurs in human glioma and enhances cellular motility via the epidermal growth factor receptor（EGFR）pathway. However, the underlying molecular mechanism is not fully understood. In this study, we found that Migfilin promoted matrix metalloproteinase-2（MMP-2） activity, and restrained the expression of tissue inhibitor of metalloproteinase 2（TIMP2）, which is an MMP-2 inhibitor. Functional and structural studies showed that the LIM1 domain of Migfilin was required for Migfilin-mediated TIMP2 expression inhibition and MMP-2 activity, and was also necessary in promoting cell motility. Furthermore, Migfilininduced EGFR phosphorylation was greatly reduced by MMP-2 inhibitor（GM6001） or si RNA, while Migfilin-induced MMP-2 activation was also blocked by the EGFR inhibitor（AG1478） or si RNA. MMP-2 and EGFR inhibitors and their si RNAs can block Migfilin-induced migration and invasion, *** results demonstrated that EGFR and MMP-2 signalings may form a positive feedback loop to enhance Migfilin-induced migration and invasion. Finally, we detected that the expression of Migfilin,EGFR phosphorylation（Tyr1173） and MMP-2 activity had a positive correlation in the clinical glioma sample. Taken together, these results suggest that Migfilin is a critical regulator in cellular motility by driving the EGFR-MMP-2 feedback loop, and may be considered as a potential therapeutic target in glioma.

关键词： Glioma Migfilin MMP-2 EGFR Motility

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α-parvin controls chondrocyte column formation and regulates long bone development

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骨研究:英文版 2023年第3期11卷 638-649页

作者： Jifan Yuan Ling Guo Jiaxin Wang Zhongjun Zhou chuanyue wu Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research Shenzhen Key Laboratory of Cell MicroenvironmentDepartment of BiologySchool of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China School of Biomedical Sciences Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong 999077China Shenzhen Key Laboratory of Epigenetics and Precision Medicine for Cancers National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital&Shenzhen HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeShenzhenChina Department of Pathology School of MedicineUniversity of PittsburghPittsburghPA 15261USA

Endochondral ossification requires proper control of chondrocyte proliferation,differentiation,survival,and *** we show that knockout ofα-parvin,an integrin-associated focal adhesion protein,from murine limbs causes defects in endochondral ossification and *** mutant long bones were shorter but wider,and the growth plates became disorganized,especially in the proliferative *** two-photon time-lapse imaging of bone explant culture,we provide direct evidence showing thatα-parvin regulates chondrocyte rotation,a process essential for chondrocytes to form columnar ***,loss ofα-parvin increased binucleation,elevated cell death,and caused dilation of the resting zones of mature growth ***-cell RNA-seq analyses revealed alterations of transcriptome in all three zones(i.e.,resting,proliferative,and hypertrophic zones)of the growth *** results demonstrate a crucial role ofα-parvin in long bone development and shed light on the cellular mechanism through whichα-parvin regulates the longitudinal growth of long bones.

关键词： chondrocyte alterations elevated

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Akt isoforms differentially provide for chemoresistance in prostate cancer

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Cancer Biology & Medicine 2022年第5期19卷 635-650页

作者： Bo Ma Hanshuang Shao Xia Jiang Zhou Wang chuanyue(Cary)wu Diana Whaley Alan Wells Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy Cancer InstituteXuzhou Medical UniversityXuzhou 221002China Department of Pathology University of PittsburghPittsburghPA 15261USA Pittsburgh VA Healthcare System PittsburghPA 15213USA Department of Biomedical Informatics University of PittsburghPittsburghPA 15261USA Department of Urology University of PittsburghPittsburghPA 15261USA Department of Pharmacology and Chemical Biology University of PittsburghPittsburghPA 15261USA UPMC Hillman Cancer Center PittsburghPA 15232USA

Objective:Early prostate cancer micrometastatic foci undergo a mesenchymal to epithelial reverting transition,not only aiding seeding and colonization,but also rendering the tumor cells generally *** previously found that upregulated E-cadherin in the epithelial micrometastases activated canonical survival pathways,including PI3K-Akt,that protected the tumor cells from death;however,the extent of protection from blocking the pathway in its entirety was modest,because different isoforms may have alternately affected cell ***,we characterized Akt isoform expressions in primary and metastatic prostate cancers,as well as their individual contributions to ***:Akt isoforms and E-cadherin were manipulated with drugs,knocked down,and over *** cell killing was determined in vitro and in *** survival was calculated from patient records and ***:Pan-Akt inhibition sensitized tumor cells to chemotherapy,and specific blockade of Akt1 or/and Akt2 caused cells to be more *** of Akt3 induced apoptosis.A low dose of Akt1 or Akt2 inhibitor enabled standard chemotherapies to significantly eradicate metastatic prostate tumors in a mouse model,acting as *** human specimens,we found Akt1 and Akt2 positively correlated,whereas Akt3 inversely correlated,with the overall survival of prostate cancer ***1high/Akt2high/Akt3low tumors had the worst ***:E-cadherin-induced activation of Akt1/2 isoforms was the essential mechanism of chemoresistance,whereas Akt3 made cells more *** findings emphasized the need to target Akt1/2,rather than pan-Akt,as a rational therapeutic approach.

关键词： Chemoresistance adjuvant therapy metastasis dormancy Akt isoforms

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Focal adhesion protein Kindlin-2 regulates bone homeostasis in mice

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Bone Research 2020年第1期8卷 26-38页

作者： Huiling Cao Qinnan Yan Dong Wang Yumei Lai Bo Zhou Qi Zhang Wenfei Jin Simin Lin Yiming Lei Liting Ma Yuxi Guo Yishu Wang Yilin Wang Xiaochun Bai Chuanju Liu Jian QFeng chuanyue wu Di Chen Xu Cao Guozhi Xiao Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research Shenzhen Key Laboratory of Cell Microenvironmentand Department of BiologySouthern University of Science and TechnologyShenzhen 518055China Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education Co-innovation Center of NeuroregenerationNantong UniversityNantong 226001China Department of Orthopedic Surgery Rush University Medical CenterChicagoIL 60612USA Department of Cell Biology School of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515China Department of Orthopedic Surgery New York University School of MedicineNew YorkNY 10003USA Department of Cell Biology New York University School of MedicineNew YorkNY 10016USA Department of Biomedical Sciences Texas A&M University College of DentistryDallasTX 75246USA Department of Orthopedic Surgery The Johns Hopkins UniversityBaltimoreMD 21205USA

Our recent studies demonstrate that the focal adhesion protein Kindlin-2 is critical for chondrogenesis and early skeletal development. Here, we show that deleting Kindlin-2 from osteoblasts using the 2.3-kb mouse Col1 a1-Cre transgene minimally impacts bone mass in mice, but deleting Kindlin-2 using the 10-kb mouse Dmp1-Cre transgene, which targets osteocytes and mature osteoblasts, results in striking osteopenia in mice. Kindlin-2 loss reduces the osteoblastic population but increases the osteoclastic and adipocytic populations in the bone microenvironment. Kindlin-2 loss upregulates sclerostin in osteocytes,downregulates β-catenin in osteoblasts, and inhibits osteoblast formation and differentiation in vitro and in vivo. Upregulation ofβ-catenin in the mutant cells reverses the osteopenia induced by Kindlin-2 deficiency. Kindlin-2 loss additionally increases the expression of RANKL in osteocytes and increases osteoclast formation and bone resorption. Kindlin-2 deletion in osteocytes promotes osteoclast formation in osteocyte/bone marrow monocyte cocultures, which is significantly blocked by an anti-RANKLneutralizing antibody. Finally, Kindlin-2 loss increases osteocyte apoptosis and impairs osteocyte spreading and dendrite ***, we demonstrate an important role of Kindlin-2 in the regulation of bone homeostasis and provide a potential target for the treatment of metabolic bone diseases.

关键词： homeostasis Kindlin cytes

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基于Diffusers框架的电商产品图制作方法研究

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无线互联科技 2024年第2期21卷 104-106页

作者：伍海洋马川越刘登辉西京学院电子信息学院陕西西安710123

基于Diffusers框架与Control Net网络,文章开发并实现了服装产品图的制作流程,将Segment Anything与Grounding DINO结合,实现了自动分割衣服蒙版。将Semantic Segmentation与ViTmatte结合,实现了对皮肤的精细化分割。文章使用大模型的... 详细信息

基于Diffusers框架与Control Net网络,文章开发并实现了服装产品图的制作流程,将Segment Anything与Grounding DINO结合,实现了自动分割衣服蒙版。将Semantic Segmentation与ViTmatte结合,实现了对皮肤的精细化分割。文章使用大模型的低秩适应实现了对图像风格的微调,利用Ultralytics框架,结合Diffusers提出了一种图像修复方法。将生成图与原图对比,实验结果表明:将Diffusers与Control Net网络结合,能够实现模特替换与背景替换,该方法可以为用户提供衣服上身的参考,降低商家的产品图制作成本。

关键词：电商产品图模特替换 Diffusers Control Net

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激光粒度仪测定超细碳化硅粉体粒度研究

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无机盐工业 2016年第3期48卷 66-68页

作者：胡继林胡传跃田修营彭红霞吴腾宴湖南人文科技学院化学与材料科学系湖南娄底417000

以蒸馏水为分散介质,采用激光粒度仪对超细碳化硅粉体粒径进行测定。考察了超声波分散功率、超声波分散时间、分散剂种类、分散剂用量、遮光比等因素对超细碳化硅粉体粒径测定的影响。确定了测试超细碳化硅粉体粒径的最佳条件:超声波分... 详细信息

以蒸馏水为分散介质,采用激光粒度仪对超细碳化硅粉体粒径进行测定。考察了超声波分散功率、超声波分散时间、分散剂种类、分散剂用量、遮光比等因素对超细碳化硅粉体粒径测定的影响。确定了测试超细碳化硅粉体粒径的最佳条件:超声波分散功率为200 W,超声波分散时间为10 min,选择质量浓度为2.0 g/L的聚乙二醇2000作为分散剂,遮光比为10.0%。在此条件下超细碳化硅粉体的粒度分析结果重现性好、数据比较准确。

关键词：激光粒度仪碳化硅粒度分析分散剂

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密闭式热场下<111>晶向Si单晶高晶转生长研究

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半导体技术 2011年第2期36卷 136-139页

作者：刘锋韩焕鹏李丹王世援吴磊周传月莫宇中国电子科技集团公司第四十六研究所天津300220

在Kayex CG6000单晶炉上采用优化的350 mm密闭式热场,在23~25 r/min高晶转下用直拉法拉制出了Φ76.2~125 mm、n型高阻〈111〉晶向Si单晶,单晶的外形和径向电阻率均匀性良好。对〈111〉晶向Si单晶在高晶转下生长容易出现扭曲变形、棱面... 详细信息

在Kayex CG6000单晶炉上采用优化的350 mm密闭式热场,在23~25 r/min高晶转下用直拉法拉制出了Φ76.2~125 mm、n型高阻〈111〉晶向Si单晶,单晶的外形和径向电阻率均匀性良好。对〈111〉晶向Si单晶在高晶转下生长容易出现扭曲变形、棱面较宽现象的原因进行了分析。通过添加热屏,加强热场的保温和热屏的隔热作用,及缩小等径生长阶段熔Si液面与热屏间的距离,提高了晶体结晶前沿的温度梯度。从而避免了〈111〉晶向Si单晶的扭曲变形,减小了单晶棱面宽度,同时有利于消除晶体的漩涡缺陷。

关键词：硅单晶〈111〉晶向直拉法高晶转密闭式热场

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渗透型防水材料对混凝土耐久性的影响研究

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施工技术 2016年第9期45卷 80-83页

作者：沈川越黄佳健汪群林航葳吴皓蒋一彬张文华浙江工业大学建筑工程学院浙江杭州310014

为研究渗透型防水材料对混凝土耐久性的影响,对分别经过涂刷水性渗透型无机防水剂、浸润水性渗透型无机防水剂、涂刷水泥基渗透结晶型防水涂料处理及未经防水材料处理的基准混凝土试件进行了NEL法快速氯离子扩散试验、碳化试验、冻融试... 详细信息

为研究渗透型防水材料对混凝土耐久性的影响,对分别经过涂刷水性渗透型无机防水剂、浸润水性渗透型无机防水剂、涂刷水泥基渗透结晶型防水涂料处理及未经防水材料处理的基准混凝土试件进行了NEL法快速氯离子扩散试验、碳化试验、冻融试验。试验结果表明,涂刷水泥基渗透结晶型防水涂料对抗氯离子渗透的效果最好,浸润水性渗透型无机防水剂对抗冻融的效果最好,3种防水处理方式对抗碳化效果均不好。通过扫描电镜微观观察证明了防水材料的渗透结晶效果。

关键词：防水工程混凝土渗透型防水材料耐久性试验

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渗透型防水材料对混凝土抗氯离子渗透性能的影响

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新型建筑材料 2015年第11期42卷 81-83页

作者：沈川越汪群黄佳健林航葳吴皓蒋一彬张文华浙江工业大学建筑工程学院浙江杭州310014

为研究渗透型防水材料对混凝土抗氯离子渗透性能的影响,对分别经过涂刷水性渗透型无机防水剂、浸润水性渗透型无机防水剂、涂刷水泥基渗透结晶型防水涂料处理及未经防水材料处理的基准混凝土试件进行了快速氯离子扩散性能试验。采用NEL... 详细信息

为研究渗透型防水材料对混凝土抗氯离子渗透性能的影响,对分别经过涂刷水性渗透型无机防水剂、浸润水性渗透型无机防水剂、涂刷水泥基渗透结晶型防水涂料处理及未经防水材料处理的基准混凝土试件进行了快速氯离子扩散性能试验。采用NEL法测试上述4种试件的氯离子扩散系数。试验结果表明,水泥基渗透结晶型防水涂料的效果比水性渗透型无机防水剂更佳。通过扫描电镜微观观察证实了防水材料的渗透结晶效果。

关键词：混凝土渗透型防水材料氯离子扩散:NEL法

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硅胶负载氨基硫脲的合成及对Ag+和Hg2+的吸附性能研究

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离子交换与吸附 2022年第6期38卷 490-500页

作者：吴跃吴开彦刘同河马传月程玉芹华明建牛余忠四川大学匹兹堡学院鲁东大学化学与材料科学学院

本文通过均相和异相合成法相结合，合成了硅胶负载氨基硫脲(SG-GPTS-ATS)并研究了其对Hg2+和Ag+的吸附性能。探究了溶液p H、时间、温度、金属离子初始浓度等因素对吸附性能的影响。结果表明，SG-GPTS-ATS对Hg2+和Ag+均表现良好的吸附... 详细信息

本文通过均相和异相合成法相结合，合成了硅胶负载氨基硫脲(SG-GPTS-ATS)并研究了其对Hg2+和Ag+的吸附性能。探究了溶液p H、时间、温度、金属离子初始浓度等因素对吸附性能的影响。结果表明，SG-GPTS-ATS对Hg2+和Ag+均表现良好的吸附性能，最佳吸附溶液pH为6;SG-GPTS-ATS对Hg2+和Ag+的吸附动力学符合拟二级动力学模型，且为液膜扩散控制过程；等温吸附研究表明，SG-GPTS-ATS对两种金属离子的吸附量随温度和金属离子浓度的增大而升高，吸附过程符合Langmuir模型；热力学参数表明，SG-GPTS-ATS对两种金属离子的吸附为自发、吸热的过程。

关键词：硅胶氨基硫脲合成吸附 Hg2+ Ag+

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