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Aldehyde oxidase mediated enantioselective metabolic health risk of dinotefuran

Science China Chemistry 2024年第6期67卷 2079-2091页

作者： Liwei xu Xinxin xu Hua Kuang chuanlai xu Xiaoling Wu State Key Laboratory of Food Science and Resources Jiangnan UniversityWuxi214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and Technology Jiangnan UniversityWuxi214122China Collaborative Innovation center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityWuxi214122China

Chiral pollutants often pose significant differential environmental health *** this study,the biotransformation of chiral dinotefuran(DIN)and its enantioselective metabolic toxicity mechanisms have been systemically ***,reversedphase chromatography-high resolution mass spectrometry was developed to quantify the content of DIN R/S chiral enantiomer with pg level sensitivity,revealing a lower elimination rate constant(K_(e))of S-DIN(0.730 h^(-1))than R-DIN(0.746 h^(-1)).Secondly,the interaction mechanism between DIN metabolism and important endogenous bioactive molecules,such as aldehyde oxidase(AOX)and neurotransmitters,was *** DIN nitro-group was converted into a guanidine group by the reducing site of nearby flavin adenine dinucleotide(FAD)in AOX with the preferred higher affinity of ***,the endogenous tryptophan(Trp)aldehyde metabolic intermediate,5-hydroxyindoleacetaldehyde(5-HIAL),provides a persistent electron donor for DIN reduction via the oxidation-catalyzed site in AOX,resulting in remarkable up-regulation of monoamine neurotransmitters such as serotonin and ***,the higher level of neurotransmitters further mediated dysregulation of oxylipin homeostasis via the serotonergic pathway,where S-DIN exhibited more pronounced liver lipid damage and environmental health risk with the accumulated lipid biomarkers,oxidized triglyceride(OxTG)and oxidized sphingomyelin(OxSM).This study elucidates the AOX-mediated enantioselectivity metabolic pathway of DIN,providing a new analytical method for chiral pollutants and paves the way for their health risk assessments.

关键词： neonicotinoid dinotefuran enantiomer aldehyde oxidase metabolomics health risk

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Circularly Polarized Light-Enabled Chiral Nanomaterials:From Fabrication to Application

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Nano-Micro Letters 2023年第3期15卷 171-189页

作者： Changlong Hao Gaoyang Wang Chen Chen Jun xu chuanlai xu Hua Kuang Liguang xu International Joint Research Laboratory for Biointerface and Biodetection State Key Lab of Food Science and TechnologySchool of Food Science and TechnologyJiangnan UniversityWuxiJiangsu 214122People’s Republic of China Department of Neurology China National Clinical Research Center for Neurological DiseasesBeijing Tiantan HospitalCapital Medical UniversityNo.119 South 4Th Ring West RoadBeijing 100070People’s Republic of China

For decades,chiral nanomaterials have been extensively studied because of their extraordinary *** nanostructures have attracted a lot of interest because of their potential applications including biosensing,asymmetric catalysis,optical devices,and negative index *** polarized light(CPL)is the most attractive source for chirality owing to its high availability,and now it has been used as a chiral source for the preparation of chiral *** this review,the recent progress in the field of CPL-enabled chiral nanomaterials is ***,the recent advancements in the fabrication of chiral materials using circularly polarized light are described,focusing on the unique ***,an overview of the potential applications of chiral nanomaterials driven by CPL is provided,with a particular emphasis on biosensing,catalysis,and ***,a perspective on the challenges in the field of CPL-enabled chiral nanomaterials is given.

关键词： Circularly polarized light Chiral Nanomaterials Fabrication Application

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Gold-nanoparticle-based multiplex immuno-strip biosensor for simultaneous determination of 83 antibiotics

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Nano Research 2023年第1期16卷 1259-1268页

作者： Xianlu Lei Xinxin xu Liqiang Liu Liguang xu Li Wang Hua Kuang chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Antibiotic residues,generated by the irrational use of drugs and environmental pollution,have always been a great challenge to aquaculture ***,a quick,convenient,and performance-excellent way to detect antibiotic residues in aquaculture fish is urgently *** this study,a multiplex immunochromatographic strip biosensor based on gold nanoparticles was developed for the simultaneous detection of five classes of antibiotic residues(24β-lactam antibiotics,26 sulfonamides,five tetracyclines,24 quinolones,and four amphenicols)in aquaculture fish within 10 *** detection ranges of five representative antibiotics,penicillin G,sulfamethazine,tetracycline,enrofloxacin,and chloramphenicol,were 2.33–38.4,0.688–17.1,1.4–48.1,1.45–32.9,and 0.537–9.06μg/kg,*** accuracy and stability of these measurements were demonstrated by analyzing spiked fish samples,with recovery rates of 87.5%–115.2%and a coefficient of variation<9.5%.The cross-reaction rates,based on the five representative antibiotics,were 3.77%–202%forβ-lactams,3.95%–137%for sulfonamides,9.19%–100%for tetracyclines,4.9%–145%for quinolones,and 3.2%–100%for *** excellent testing performance of the biosensor strip to most of antibiotic residues in aquaculture fish ensures they meet the maximum residue limits required by countries or ***,this multiplex immunochromatographic strip biosensor is potentially applicable to the rapidly on-site determination of antibiotic residues in aquaculture fish.

关键词： antibiotic aquaculture biosensor immunochromatographic strip

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Multiple detection of 15 triazine herbicides by gold nanoparticle based-paper sensor

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Nano Research 2022年第6期15卷 5483-5491页

作者： Lingling Guo Xinxin xu Jing Zhao Shudong Hu Liguang xu Hua Kuang chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China Department of Radiology Affiliated HospitalJiangnan UniversityWuxi 214122China

Triazine herbicides have been widely used in agriculture,but their residues can harm the environment and human *** help monitor these,we have developed an effective immunochromatographic strip test that can simultaneously detect 15 different triazines in grain samples(including ametryn,cyprazine,atraton,prometon,prometryn,atrazine,propazine,terbuthylazine,simetryn,trietazine,secbumeton,simazine,desmetryn,terbumeton and simetone).Based on our optimization procedure,the visual limit of detection(vLOD)for these triazines was found to be 2–10 ng/mL in assay buffer,and 0.02–0.1 mg/kg in grain *** different grain matrices including corn,brown rice,wheat,and sorghum were studied and the test results showed no significant differences between the 15 triazines analyzed using this *** test is simple,convenient,rapid,and low-cost,and could be an effective tool for primary screening of triazine residues in grain samples.

关键词： triazine herbicides immunochromatographic strip gold nanoparticles grain

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Gold-based lateral-flow strip for the detection of penconazole in watermelon and cucumber samples

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Food Quality and Safety 2022年第2期6卷 257-264页

作者： Yang Liu Xinxin xu Liqiang Liu Liguang xu Hua Kuang chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxiChina International Joint Research Laboratory for Biointerface and Biodetection School of Food Science and TechnologyJiangnan UniversityWuxiChina

As a typical triazole fungicide,penconazole(PEN)is widely used in agriculture but has been proven to be *** this study,we designed a new hapten to prepare a highly sensitive and specific anti-PEN monoclonal antibody(mAb)and established a gold nanoparticle-based lateral-flow immunoassay(LFIA)for the detection of PEN residues in watermelon and *** 50%inhibitory concentration(IC_(50))of the mAb was 0.42 ng/mL and the LFIA strip had a visual limit of detection(vLOD)of 2.5 ng/g and a cut-off value of 10 ng/g in watermelon and *** calculated limit of detection(LOD)of the LFIA strip was 0.36 ng/g for watermelon and 0.29 ng/g for *** LFIA strip also gave a re-covery rate of 92.5%-109.0%for watermelon samples and 92.5%-106.7%for cucumber *** results using the LFIA strip are highly consistent with those seen using liquid chromatography-tandem mass *** our developed LFIA strip represents a potentially reliable tool for the rapid on-site screening for PEN in watermelons and cucumbers.

关键词： Penconazole monoclonal antibody lateral flow immunoassay watermelon cucumber

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Quantitative assessment of the breast cancer marker HER2 using a gold nanoparticle-based lateral flow immunoassay

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Nano Research 2024年第6期17卷 5452-5460页

作者： Liya Ye Xinxin xu Aihua Qu Liqiang Liu chuanlai xu Hua Kuang International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Human epidermal growth factor receptor 2(HER2)is an important biomarker for detection and treatment of breast *** this study,we developed monoclonal antibodies against the extracellular domain(ECD)of HER2 and established a rapid and accurate lateral flow immunoassay(LFIA)for use in community medical *** gene sequence of human HER2-ECD was obtained from the National Center for Biotechnology Information(NCBI)to construct the expression ***2-ECD protein expressed in HEK293F cells was used to immunize BALB/c *** monoclonal antibodies were produced in mouse ascites and isolated by hybridoma cell *** were analyzed for purity by SDS-PAGE(sodium dodecyl sulphate-polyacrylamide gel-electrophoresis)and affinity was assessed by enzyme-linked immunosorbent assay(ELISA)while subtypes were detected using the commercial *** HER2-ECD test strip was prepared based on the sandwich method and evaluated using a portable detection *** affinity of the paired antibodies,4D8 and 8D9,both reached 1×108 L/*** antibodies specifically recognized the HER2-ECD protein in *** limit of detection(LOD)of the gold nanoparticle(AuNP)-based LFIA was 1.7 ng/mL with a detection range of 1.7-400 ng/mL,and the performance of the HER2-ECD strip correlated well with that of a Siemens chemiluminescent immunoassay(CLIA)*** conclusion,the paired antibodies were successfully prepared with high affinity and *** AuNP-based LFIA of HER2-ECD provides a fast and accurate method to detect the concentration of HER2-ECD in serum samples for clinical use in community medical institutions,and could contribute to determining the progress of the disease or the effectiveness of treatment.

关键词： human epidermal growth factor receptor 2 breast cancer monoclonal antibody quantitative lateral flow immunoassay

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A gold-based multiplex immunochromatographic sensor for detecting paclitaxel

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Nano Research 2024年第7期17卷 6420-6429页

作者： Xiaoqian Jiang Aihua Qu Xinxin xu Hua Kuang Liqiang Liu chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Paclitaxel(PTX),methotrexate(MTX),and 5-fluorouracil(5-FU)are commonly-used small molecule anti-tumor drugs for breast ***,drug resistance occurs with long-term treatment or excessive use,and the high concentrations of PTX,MTX,and 5-FU in patients may lead to side effects with dose-limiting toxicities,such as myelosuppression,hepatotoxicity,and peripheral ***,concentration monitoring of PTX,MTX,and 5-FU in clinical treatment is very *** prepared highly sensitive and specific monoclonal antibodies using several haptens,and established a multiple paper-based sensor utilizing optical signals of gold nanoparticles,which simultaneously detected PTX,MTX,and 5-FU in human plasma or urine with 10 min.A portable scanner for quantitative detection in plasma and urine samples was used,and the calculated limit of detection(cLOD)values were 0.002 and 0.142μg/mL for PTX,0.187 and 0.976 ng/mL for MTX,and 0.057 and 0.128μg/mL for 5-FU,*** the recovery test,the recovery rates and the coefficient of variation were 85.84%–108.81%and 1.23%–8.80%,respectively,indicating the reliability and accuracy of the multiple gold immunochromatographic strip(GIS).In addition,for the determination of collected clinical plasma samples,the correlation between the test data of the multiple GIS and liquid chromatography–tandem mass spectrometry(LC–MS/MS)was ***,the developed multiple GIS could be applied to the rapid detection of PTX,MTX,and 5-FU in different clinical samples.

关键词： paclitaxel methotrexate 5-fluorouracil immunochromatographic assay plasma urine

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A quadruplex immunochromatographic assay for the ultrasensitive detection of 11 anesthetics

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Nano Research 2023年第8期16卷 11269-11277页

作者： Xianlu Lei Xinxin xu Li Wang Wei Zhou Liqiang Liu Liguang xu Hua Kuang chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China Jiangsu Product Quality Testing and Inspection Institute Nanjing 210000China

Anesthetic residues in fish represent a potential risk to human ***,it is important to develop a sensitive and broad-specific method for the detection of *** this study,we developed a colloidal gold-based quadruplex immunochromatographic(Qua-ICS)assay using four highly sensitive monoclonal antibody immunotherapy(mAbs)that simultaneously detected 11 anesthetic residues in fish within 10 *** colorimetric and cut-off values(COVs)for procaines,eugenols,bupivacaines,and tricaine(TMS)were 0.37–1.1 and 3.3–10,11–222 and 100–2000,0.37 and 3.3,and 111 and 10,000µg/kg,*** analysis was achieved with a portable strip-reader,and the detection ranges were 0.15–2.6,6.3–677,0.13–2.8,and 83–1245µg/kg for procaines,eugenols,bupivacaines,and tricaine,*** developed method was reliable and accurate according to the recovery test results and analyses of real ***,the strip can be used as an alternative method for the rapid detection of anesthetic residues in fish.

关键词： bupivacaine colloidal gold eugenol procaine tricaine(TMS)

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Chiral Nanoprobes and Their Biological Effects

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Chinese Journal of Chemistry 2021年第1期39卷 25-31页

作者： Maozhong Sun Si Li Changlong Hao chuanlai xu Hua Kuang International Joint Research Laboratory for Biointerface and Biodetection Jiangnan UniversityWuxiJiangsu 214122China State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxiJiangsu 214122China

Chirality is an essential property of *** emergence of chiral material in nanoscale has shown great promise in the investigation of biological interfaces,chiral optical devices,metamaterials and the origin of *** the present study,we focused on fabricating chiral nanoprobes and their applications in living *** precise synthesis or the self-assembly of various chiral configurations,significant research achievements have been obtained by our *** this brief introduction,we will discuss the recent progress of chiral nanostructures in the selective recognition of biomolecules,ultrasensitive detection and their interaction with living cells.

关键词： Chirality Self-assembly Nanoparticle Cell Detection Biomolecule

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Synthesis of haptens and gold-based immunochromatographic paper sensor for vitamin B_(6) in energy drinks and dietary supplements

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Nano Research 2022年第3期15卷 2479-2488页

作者： Lu Zeng Xinxin xu Shanshan Song Liguang xu Liqiang Liu Jing Xiao chuanlai xu Hua Kuang State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China NHC Key Laboratory of Food Safety Risk Assessment China National Center for Food Safety Risk AssessmentBeijing 100022China

We designed and synthesized novel haptens to produce monoclonal antibodies(mAb)against vitamin B_(6)(VB_(6)).A group-specific mAb(2F9)that recognized pyridoxine(PN),pyridoxamine(PM),and pyridoxal(PL)was prepared using a homologous strategy with 50%maximal inhibitory concentration(IC_(50))values of 106.60,250.57,and 400.11 ng/mL,*** on this,a gold nanoparticles(AuNPs)-based immunochromatographic strip(ICS)test was established for the detection of VB_(6) in energy drinks and B-vitamin complex *** developed ICS test results could be semi-quantitatively evaluated by the naked eye within 10 min,and displayed the visual limit of detection(vLOD)values of 250,500,and 1,000 ng/mL for PN,PM,and PL,*** quantitative analysis,the results obtained by strip reader,with calculated LOD values for PN,PM,and PL were 14.10,55.58,and 56.25 ng/mL,*** energy drinks and B-vitamin complex tablet samples were detected by the strips and the results were confirmed with high-performance liquid ***,the developed AuNPs-based immunochromatographic sensor was suitable and promising for the group-specific recognition and rapid detection of VB6 in fortified foods and dietary supplements.

关键词： vitamin B_(6) monoclonal antibody immunochromatographic strip test

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