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清代安西卫、沙州卫设置时间考——兼论沙州所建制的有无

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敦煌研究 2015年第3期 104-108页

作者：刘传飞中国人民大学清史研究所北京100872

安西卫和沙州所设置于雍正二年(1724),雍正四年(1726)沙州所升卫,并非是长期以来被学界和社会所广泛接受的"雍正元年(1723)置安西卫、沙州所,雍正三年(1725)沙州所升卫"。对文献解读方式的正确与否、文献形成过程的了解程度和对地区自... 详细信息

安西卫和沙州所设置于雍正二年(1724),雍正四年(1726)沙州所升卫,并非是长期以来被学界和社会所广泛接受的"雍正元年(1723)置安西卫、沙州所,雍正三年(1725)沙州所升卫"。对文献解读方式的正确与否、文献形成过程的了解程度和对地区自身发展脉络的熟悉程度都对学术成果的可信度产生一定的影响。

关键词：安西卫沙州所沙州卫敦煌

Modification of P450 Monooxygenase into its Peroxygenase Mode

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Modification of P450 Monooxygenase into its Peroxygenase Mod...

体验经济视角下服务场景对顾客满意影响的实证

第十一届全国化学生物学学术会议

作者： Jie Chen Nana Ma Zhifeng Chen Panxia Zhao Xiling Wang Fanhui Kong chuanfei liu Mohnad Abdalla Abdalgader Mohamed 丛志奇 Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences 中科院青岛生物能源与过程研究所

Cytochrome P450 monooxygenase are synthetically useful enzymes and promising versatile oxidative biocatalysts,which catalyze the monooxygenation of various organic ***,the practical use of P450 is limited by its co-enzyme NAD(P)H and complex electron transport *** HO can simple the catalytic cycle but suffer from poor activity of most P450 in the presence of *** overcome the problem,by mimicking the catalytic mechanism of natural fungal peroxygenase,we have constructed a unique strategy for the modification of P450 monooxygenase into its peroxygenase mode,resulting in versatile oxidation of non-native substrates in assistance of dual-functional small molecule(DFSM). This system afforded the best peroxygenase activity for epoxidation and sulfoxidation among those P450-HO system previously *** approach expanded the potential use of P450 s in organic *** combination with protein engineering,the DFSM-facilitated P450 peroxygenase has been used for the hydroxylation of inert aromatic and aliphatic C-H bond.

关键词： P450 monooxygenase peroxygenase C-H activation oxidation hydrogen peroxide

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科技管理研究 2014年第6期34卷 197-200页

作者：李祗辉白玮马瑛孙希红刘传菲北京石油化工学院人文社科学院北京102617

以北京星巴克咖啡厅为例,对服务场景与顾客满意的影响关系进行实证研究。在回顾文献的基础上,建立模型和假设,利用自行开发的问卷收集数据进行分析处理。研究结果表明,服务场景对顾客满意有显著影响,4个因子中座位与氛围因子、视觉与位... 详细信息

以北京星巴克咖啡厅为例,对服务场景与顾客满意的影响关系进行实证研究。在回顾文献的基础上,建立模型和假设,利用自行开发的问卷收集数据进行分析处理。研究结果表明,服务场景对顾客满意有显著影响,4个因子中座位与氛围因子、视觉与位置功能因子和清洁性因子的影响程度相似,听觉与嗅觉因子的影响程度排在第四位。研究结果对于企业营销实践有重要的指导和参考价值。

关键词：服务场景顾客满意服务营销体验经济

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智慧家庭的VR全景视频业务实现

电信科学 2017年第10期33卷 185-193页

作者：罗传飞孔德辉刘翔凯徐科杨浩中国电信股份有限公司上海研究院上海200122 深圳市中兴微电子技术有限公司广东深圳518081

全景视频作为一种新的视频格式极大程度地改变了用户观看视频的方式,可以提升智慧家庭在视频领域的用户体验。IPTV良好的解码能力以及优异的网络支撑等优势对全景视频的应用推广具有积极作用。结合VR全景视频的特点,分析了超高分辨率全... 详细信息

全景视频作为一种新的视频格式极大程度地改变了用户观看视频的方式,可以提升智慧家庭在视频领域的用户体验。IPTV良好的解码能力以及优异的网络支撑等优势对全景视频的应用推广具有积极作用。结合VR全景视频的特点,分析了超高分辨率全景视频在IPTV平台中的技术挑战,同时基于FOV视点自适应框架,结合MCTS编码、多分辨率和多tile视频分发和终端根据视点部分解码tile技术,给出一种IPTV超高分辨率全景业务实现方案。

关键词：智慧家庭全景视频 IPTV 虚拟现实

发根农杆菌T-DNA基因对3种葛属植物毛状根形态和葛根素含量的影响

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应用与环境生物学报 2001年第2期7卷 143-146页

作者：刘传飞李玲潘瑞炽金乐红江西农业大学农学院南昌330045 华南师范大学生物系广州510631

野葛、山葛和三裂叶葛的离体叶片与发根农杆菌R16 0 1直接感染诱导出毛状根并能够合成葛根素 ,毛状根离体培养后均出现两种形态 :一种是有较高的葛根素含量的典型毛状根 ,另一种是葛根素含量低但生长速率较快的愈伤组织化毛状根 .两类... 详细信息

野葛、山葛和三裂叶葛的离体叶片与发根农杆菌R16 0 1直接感染诱导出毛状根并能够合成葛根素 ,毛状根离体培养后均出现两种形态 :一种是有较高的葛根素含量的典型毛状根 ,另一种是葛根素含量低但生长速率较快的愈伤组织化毛状根 .两类毛状根均有rolB基因存在 ,表明rolB基因对所有毛状根的诱导和生长是必需的 .aux1存在于所有愈伤组织化毛状根 ,而在典型毛状根中的检出率为 2 0 %～ 5 0 % ,表明aux1基因有诱导毛状根产生愈伤组织的作用 .含有ags基因的毛状根葛根素含量低于不含ags基因的毛状根葛根素含量 ,表明ags基因不利于次生代谢物合成 .表3参

关键词：发根农杆菌葛属毛状根形态葛根素遗传转化 T-DNA基因药用植物

生长素和细胞分裂素物质对野葛外植体器官发生的影响

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华南师范大学学报（自然科学版） 1999年第2期31卷 100-104页

作者：刘传飞李玲施和平潘瑞炽华南师范大学生物学系广州510631

通过比较几种生长素和细胞分裂素物质对野葛（ｐｕｅｒａｒｉａｌｏｂａｔａ）外植体形态发生的影响发现，ＢＡ诱导的不定芽数量和质量均好于ＫＴ，最适浓度１ｍｇ·Ｌ１，同样浓度的ＢＡ比ＫＴ易使野葛外植体基部膨大和褐化，ＫＴ比... 详细信息

通过比较几种生长素和细胞分裂素物质对野葛（ｐｕｅｒａｒｉａｌｏｂａｔａ）外植体形态发生的影响发现，ＢＡ诱导的不定芽数量和质量均好于ＫＴ，最适浓度１ｍｇ·Ｌ１，同样浓度的ＢＡ比ＫＴ易使野葛外植体基部膨大和褐化，ＫＴ比ＢＡ更易使不定芽玻璃化，提高ＢＡ浓度或者不定芽在含ＢＡ或ＫＴ的培养基上继代，也会产生玻璃化和褐化，随继代次数增加而加重－在３种生长素类物质ＩＡＡ、ＩＢＡ和ＮＡＡ诱导不定根形成过程中，在０－２ｍｇ·Ｌ－１以下，ＮＡＡ诱导的效果最好，在０－３～０－４ｍｇ·Ｌ－１的浓度范围内，ＩＢＡ效果最好，而大于０－５ｍｇ·Ｌ－１的浓度则ＩＡＡ效果最好，在同样浓度条件下，ＮＡＡ诱导的不定根粗短，而ＩＢＡ和ＩＡＡ诱导的不定根细长，多分枝，形态上较接近自然根－３种生长素类物质诱导愈伤组织和褐化能力依次为ＮＡＡ＞ＩＢＡ＞ＩＡＡ，低浓度下生长素物质诱导的不定根生长快、生长质量好，幼苗移栽成活率高，而过高的生长素物质诱导的根系粗短，易褐化和形成愈伤组织、幼苗移栽不易成活，反映不定根与外植体维管束联系差－当ＢＡ与ＩＢＡ混合使用时，ＢＡ会严重抑制ＩＢＡ诱导的不定根形成，而ＩＢＡ对ＢＡ促进的不定芽分化效应作用不明显。

关键词：野葛生长素细胞分裂素不定根不定芽豆科

Superstructure transformations from hexagonal to tetragonal microplates and nested two-dimensional nanonetworks

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Chinese Science Bulletin 2014年第16期59卷 1787-1793页

作者： Jianming Zhang chuanfei Guo Ye Tian Qian liu National Center for Nanoscience and Technology Department of Physics and TcSUH University of HoustonHoustonTX 77204USA

Design and synthesis of super-nanostructures have obtained increasing attention in nanotechnology.A series of superstructured-nanonetwork and nested-nanonetwork bismuth compounds have been developed recently based on lattice-directed topotactic *** we further develop some specific superstructures by transformation within different crystalline types,such as hexagonal BiI3micro/nanoplates,tetragonal BiOI micro/nanoplates and nested two-dimensional(2D)orthogonal Bi2S3nanonetworks,based on the lattice matching between the product and *** method offers a convenient and common route in synthesizing heterojunctions and bimaterial nanostructures similarly.

关键词：上层建筑嵌套二维微孔板纳米技术六方纳米结构 Bi2S3

Organic Semiconductor Interfaces and Their Effects in Organic Solar Cells

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Chinese Journal of Chemistry 2023年第24期41卷 3792-3805页

作者： chuanfei Wang Weidong Li Qi Zeng Xianjie liu Mats Fahlman Qinye Bao School of Materials Science and Engineering Ocean University of ChinaQingdaoShandong266100 China School of Materials Science and Engineering Shanghai University of Engineering ScienceShanghai201620 China Laboratory of Organic Electronics ITNLinköping UniversityNorrköping60174 Sweden School of Physics and Electronic Science East China Normal UniversityShanghai200241 China Shanghai Frontiers Science Research Base of Intelligent Optoelectronics and Perception Institute of OptoelectronicsFudan UniversityShanghai200433 China

Energy levels and energy level alignment at interfaces play a decisive role in designing efficient and stable organic solar cells(OSCs).In this review two usually used technologies in organic photovoltaic communities for measuring energy levels of organic semiconductors,photoelectron spectroscopy and electrochemical methods,are introduced,and the relationships between the values obtained from the corresponding techniques are *** energy level and energy level alignment across the interfaces involved in solution processed organic photovoltaics are described,and the corresponding integer charge transfer model for predicting and explaining energy level alignment is *** effects of the interface properties in designing efficient binary and ternary OSCs were *** effects of environmental factors mainly including water vapor,oxygen gas and thermal annealing on energy levels and energy level alignment involved in photoactive layers,and the subsequent effects on the corresponding OSC properties are given.

关键词： Organic solar cells Interfaces Donor-acceptor systems Electron transfer Electronic Structures Efficiency

A reporter gene assay for determining the biological activity of therapeutic antibodies targeting TIGIT

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Acta Pharmaceutica Sinica B 2021年第12期11卷 3925-3934页

作者： Zhihao Fu Hongchuan liu Lan Wang chuanfei Yu Yalan Yang Meiqing Feng Junzhi Wang Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products National Institutes for Food and Drug ControlBeijing 102629China Department of Biological Medicines&Shanghai Engineering Research Center of Immunotherapeutics School of PharmacyFudan UniversityShanghai 201203China

T cell immunoglobulin and ITIM domain(TIGIT)is a novel immune checkpoint that has been considered as a target in cancer *** available bioassays for measuring the biological activity of therapeutic antibodies targeting TIGIT are restricted to mechanistic investigations because donor primary T cells are highly ***,we designed a reporter gene assay comprising two cell lines,namely,CHO-CD112-CD3 scFv,which stably expresses CD 112(PVRL2,nectin-2)and a membranebound anti-CD3 single-chain fragment variable(scFv)as the target cell,and Jurkat-NFAT-TIGIT,which stably expresses TIGIT as well as the nuclear factor of activated T-cells(NFAT)response element-controlled luciferase gene,as the effector *** anti-CD3 scFv situated on the target cells activates Jurkat-NFATTIGIT cells through binding and crosslinking CD3 molecules of the effector cell,whereas interactions between CD 112 and TIGIT prevent *** presence of anti-TIGIT mAbs disrupts their interaction,which in turn reverses the inactivation and luciferase *** and validation studies have demonstrated that this assay is superior in terms of specificity,accuracy,linearity,and *** summary,this reliable and effective reporter gene assay may potentially be utilized in lot release control,stability assays,screening,and development of novel TIGIT-targeted therapeutic antibodies.

关键词： TIGIT Therapeutic antibodies Bioactivity determination Reporter gene assay Method validation