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Dissecting the cell microenvironment of ovarian endometrioma through single-cell RNA sequencing

Science China Life Sciences 2024年

作者： Jiangpeng Wu Siyu Xia Wenting Ye Yan Sun Jing Cai Fubing Yu Haiping Wen Xiuwei Yi Taikang Li Mingwei Chen Jiayun Chen Ge Song chuanbin yang Yali Song Jigang Wang Department of Reproductive Medicine Dongguan Maternal and Child Health Care Hospital Department of Urology and Shenzhen Clinical Research Centre for Geriatrics Shenzhen People's Hospital Southern University of Science and Technology State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Artemisinin Research Center and Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Reproductive Medicine Center Foshan Women and Children Hospital State Key Laboratory of Antiviral Drugs School of Pharmacy Henan University

Ovarian endometrioma（OE）, also known as “chocolate cysts,” is a cystic mass that develops in the ovaries due to endometriosis and is a common gynecological condition characterized by the growth of endometrial tissue outside the uterus, leading to symptoms such as dysmenorrhea, pelvic pain, and infertility. However, the precise molecular and cellular mechanisms driving this pathophysiology remain largely unknown, posing challenges for diagnosis and treatment. Here, we employed integrated single-cell transcriptomic profiling of over52,000 individual cells from endometrial tissues of OE patients and healthy donors and identified twelve major cell populations. We identified notable alterations in cell type-specific proportions and molecular signatures associated with OE. Notably, the activation of IGFBP5+macrophages with pro-inflammatory properties, NK cell exhaustion, and aberrant proliferation of IQCG+and KLF2+epithelium are key features and may be the potential mechanisms underlying the pathogenesis of OE. Collectively, our data contribute to a better understanding of OE at the single cell level and may pave the way for the development of novel therapeutic strategies.

关键词： ovary high-throughput sequencing transcriptomics gene expression analysis ligand-receptor analysis

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融合用户画像的在线学习伙伴推荐方法研究

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佳木斯大学学报（自然科学版） 2024年第1期42卷 122-124,147页

作者：林萍萍杨传斌浙江师范大学教育学院浙江金华321004 浙江师范大学信息化办公室浙江金华321004

融合用户画像为学习者推荐学习伙伴,有助于解决在线学习者的孤独感问题,提高学习者的参与度和忠诚度。分析学习者特征,从基本信息、学习准备、学习风格、学习行为四个方面设计画像标签,采集网络教学平台数据,进行建模处理,利用相似度区... 详细信息

融合用户画像为学习者推荐学习伙伴,有助于解决在线学习者的孤独感问题,提高学习者的参与度和忠诚度。分析学习者特征,从基本信息、学习准备、学习风格、学习行为四个方面设计画像标签,采集网络教学平台数据,进行建模处理,利用相似度区分相似、互补学习者画像,从而为学习者推荐同、异质学习伙伴。实验结果验证了学习者画像和推荐方法的可行性。融合学习者画像推荐学习伙伴的方法更具个性化、动态化等特点,更适合网络教学环境。

关键词：用户画像学习伙伴推荐在线学习协作学习

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校园5G专网的设计与实现

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湖北师范大学学报（自然科学版） 2024年第3期44卷 46-52页

作者：杨传斌徐恬朱玮玮浙江师范大学信息技术中心浙江金华321004 浙江师范大学教育学院浙江金华321004 浙江交通技师学院图书信息中心浙江金华321015

目前校园网存在明确的地域边界,应用限制在校园网内部,外部用户需要通过各种VPN系统接入校园网,存在使用不便性能下降的问题;其次,校园内移动终端接入校园网需要大量WiFi设施,投资大效果一般。利用5G网络高速率、低时延和高并发的特点,... 详细信息

目前校园网存在明确的地域边界,应用限制在校园网内部,外部用户需要通过各种VPN系统接入校园网,存在使用不便性能下降的问题;其次,校园内移动终端接入校园网需要大量WiFi设施,投资大效果一般。利用5G网络高速率、低时延和高并发的特点,使用UPF分流技术构建校园5G融合专网,将访问校园网的数据通过5G融合网关到达校园网,扩展校园网边界,二次鉴权系统保证校园网接入安全,实现了大规模移动终端和物联网设备接入校园网,具备投资少性能好的优势。测试表明校园5G专网用户与校内用户体验一致,使用方便且性能优良。

关键词：校园网 5G网络校园5G专网二次鉴权

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Impairment of the autophagy-lysosomal pathway in Alzheimer’s diseases: Pathogenic mechanisms and therapeutic potential

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Acta Pharmaceutica Sinica B 2022年第3期12卷 1019-1040页

作者： Wei Zhang Chengchao Xu Jichao Sun Han-Ming Shen Jigang Wang chuanbin yang Department of Geriatrics Shenzhen People’s Hospital(the Second Clinical Medical CollegeJinan Universitythe First Affiliated HospitalSouthern University of Science and Technology)Shenzhen 518020China Integrated Chinese and Western Medicine Postdoctoral Research Station Jinan UniversityGuangzhou 510632China Artemisinin Research Center Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Faculty of Health Sciences University of MacaoTaipaMacao 999078China Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China

Alzheimer’s disease(AD),the most common neurodegenerative disorder,is characterized by memory loss and cognitive *** accumulation of misfolded protein aggregates including amyloid beta(Aβ)peptides and microtubule associated protein tau(MAPT/tau)in neuronal cells are hallmarks of *** far,the exact underlying mechanisms for the aetiologies of AD have not been fully understood and the effective treatment for AD is *** is an evolutionarily conserved cellular catabolic process by which damaged cellular organelles and protein aggregates are degraded via ***,there is accumulating evidence linking the impairment of the autophagy-lysosomal pathway with AD ***,the enhancement of autophagy to remove protein aggregates has been proposed as a promising therapeutic strategy for ***,we first summarize the recent genetic,pathological and experimental studies regarding the impairment of the autophagy-lysosomal pathway in *** then describe the interplay between the autophagy-lysosomal pathway and two pathological proteins,Aβand MAPT/tau,in ***,we discuss potential therapeutic strategies and small molecules that target the autophagy-lysosomal pathway for AD treatment both in animal models and in clinical ***,this article highlights the pivotal functions of the autophagy-lysosomal pathway in AD pathogenesis and potential druggable targets in the autophagy-lysosomal pathway for AD treatment.

关键词： Alzheimer’s disease(AD) Amyloid beta(Aβ)peptides MAPT/tau Autophagy-lysosomal pathway Autophagy enhancers Autophagy Mitophagy Neurodegenerative diseases

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A single-cell landscape of triptolide-associated testicular toxicity in mice

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药物分析学报:英文版 2023年第8期13卷 880-893页

作者： Wei Zhang Siyu Xia Jinhuan Ou Min Cao Guangqing Cheng Zhijie Li Jigang Wang chuanbin yang Department of Nephrology Shenzhen Key Laboratory of Kidney DiseasesShenzhen Clinical Research Centre for GeriatricsShenzhen People's Hospital(The Second Clinical Medical CollegeJinan UniversityThe First Affiliated HospitalSouthern University of Science and Technology)ShenzhenGuangdong518020China State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Artemisinin Research CenterInstitute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing10700China

Triptolide is a key active component of the widely used traditional Chinese herb medicine Tripterygium wilfordii *** triptolide exerts multiple biological activities and shows promising efficacy in treating inflammatory-related diseases,its well-known safety issues,especially reproductive toxicity has aroused ***,a comprehensive dissection of triptolide-associated testicular toxicity at single cell resolution is still ***,we observed testicular toxicity after 14 days of triptolide exposure,and then constructed a single-cell transcriptome map of 59,127 cells in mouse testes upon *** identified triptolide-associated shared and cell-type specific differentially expressed genes,enriched pathways,and ligand-receptor pairs in different cell types of mouse *** addition to the loss of germ cells,our results revealed increased macrophages and the inflammatory response in triptolide-treated mouse testes,suggesting a critical role of inflammation in triptolide-induced testicular *** also found increased reactive oxygen species(ROS)signaling and downregulated pathways associated with spermatid development in somatic cells,especially Leydig and Sertoli cells,in triptolide-treated mice,indicating that dysregulation of these signaling pathways may contribute to triptolide-induced testicular ***,our high-resolution single-cell landscape offers comprehensive information regarding triptolide-associated gene expression profiles in major cell types of mouse testes at single cell resolution,providing an invaluable resource for understanding the underlying mechanism of triptolide-associated testicular injury and additional discoveries of therapeutic targets of triptolide-induced male reproductive toxicity.

关键词： Single-cell sequence Transcriptomics Triptolide Reproduction toxicity Testis

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Emerging roles of NRBF2/PI3KC3 axis in maintaining homeostasis of brain and guts

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Neural Regeneration Research 2022年第2期17卷 323-324页

作者： Ming-Yue Wu Cui-Zan Cai chuanbin yang Zhenyu Yue Ye Chen ZhaoXiang Bian Min Li Jia-Hong Lu State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao Special Administrative RegionChina School of Chinese Medicine Hong Kong Baptist UniversityHong Kong Special Administrative RegionChina Department of Neurology and Neuroscience Friedman Brain InstituteIcahn School of Medicine at Mount SinaiNew YorkNYUSA Department of Gastroenterology State Key Laboratory of Organ Failure ResearchGuangdong Provincial Key Laboratory of GastroenterologyNanfang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina

NRBF2 has been identified as the fifth component of PI3KC3 complex and is required for maintaining the kinase activity to promote ***,the physiological and pathological roles of NRBF2are largely unknown.

关键词： PI3K homeostasis NRB

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基于冠状动脉CT血管成像评估高危斑块特征与血清高敏C反应蛋白水平的关系

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中国中西医结合影像学杂志 2024年第5期22卷 554-558页

作者：陈治宇徐晗颜欢欢蒿保利于德新杨传彬山东大学齐鲁医院放射科山东济南250012 山东省济南市第三人民医院影像中心山东济南250132 山东省公共卫生临床中心放射科山东济南250102

目的:探讨冠状动脉CT血管成像(CCTA)识别的高危斑块特征与血清高敏C反应蛋白(hs-CRP)水平的关系。方法:回顾性分析同时接受血清hs-CRP和CCTA检查的118例冠状动脉粥样硬化性心脏病(冠心病)患者。根据患者的hs-CRP水平,将其分为低水平组(h... 详细信息

目的:探讨冠状动脉CT血管成像(CCTA)识别的高危斑块特征与血清高敏C反应蛋白(hs-CRP)水平的关系。方法:回顾性分析同时接受血清hs-CRP和CCTA检查的118例冠状动脉粥样硬化性心脏病(冠心病)患者。根据患者的hs-CRP水平,将其分为低水平组(hs-CRP≤3 mg/L)62例和高水平组(hs-CRP>3 mg/L)56例。评估并比较2组CT高危斑块特征在CCTA图像上的出现率,以及临床基线资料和CCTA识别的高危斑块特征。结果:高水平组中有吸烟史患者比例(48.21%)高于低水平组(27.42%)(P=0.020)。低密度斑块和餐巾环征在高水平组中更常见(分别为64.10%vs.38.57%和28.21%vs.14.29%),而点状钙化在低水平组的发生率(27.14%)高于高水平组(14.10%)。多因素logistic回归分析结果显示,吸烟史及低密度斑块是hs-CRP水平升高的独立危险因素。结论:CCTA识别的低密度斑块和餐巾环征在hs-CRP水平较高的患者中更常见,且吸烟史与低密度斑块是血清hs-CRP水平升高的独立危险因素。

关键词：冠状动脉CT血管成像高敏C反应蛋白高危斑块

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Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1

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Acta Pharmaceutica Sinica B 2022年第5期12卷 2300-2314页

作者： Piao Luo Dandan Liu Qian Zhang Fan yang Yin-Kwan Wong Fei Xia Junzhe Zhang Jiayun Chen Ya Tian chuanbin yang Lingyun Dai Han-Ming Shen Jigang Wang Artemisinin Research Center Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Central People’s Hospital of Zhanjiang Zhanjiang 524037China Department of Urology the Second Clinical Medical CollegeJinan University(Shenzhen People’s Hospital)Shenzhen 518020China Integrated Chinese and Western Medicine Postdoctoral Research Station Jinan UniversityGuangzhou 510632China Faculty of Health Sciences University of MacaoTaipaMacao 999078China Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China

Ferroptosis is a form of regulated cell death, characterized by excessive membrane lipid peroxidation in an iron-and ROS-dependent manner. Celastrol, a natural bioactive triterpenoid extracted from Tripterygium wilfordii, shows effective anti-fibrotic and anti-inflammatory activities in multiple hepatic diseases. However, the exact molecular mechanisms of action and the direct protein targets of celastrol in the treatment of liver fibrosis remain largely elusive. Here, we discover that celastrol exerts anti-fibrotic effects via promoting the production of reactive oxygen species(ROS) and inducing ferroptosis in activated hepatic stellate cells(HSCs). By using activity-based protein profiling(ABPP) in combination with bio-orthogonal click chemistry reaction and cellular thermal shift assay(CETSA), we show that celastrol directly binds to peroxiredoxins(PRDXs), including PRDX1, PRDX2, PRDX4 and PRDX6,through the active cysteine sites, and inhibits their anti-oxidant activities. Celastrol also targets to heme oxygenase 1(HO-1) and upregulates its expression in activated-HSCs. Knockdown of PRDX1, PRDX2,PRDX4, PRDX6 or HO-1 in HSCs, to varying extent, elevated cellular ROS levels and induced ferroptosis. Taken together, our findings reveal the direct protein targets and molecular mechanisms via which celastrol ameliorates hepatic fibrosis, thus supporting the further development of celastrol as a promising therapeutic agent for liver fibrosis.

关键词： Celastrol Ferroptosis Peroxiredoxin HO-1 Hepatic fibrosis ABPP Anti-oxidant Reactive oxygen species

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Releasing Nrf2 to promote neurite outgrowth

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Neural Regeneration Research 2015年第12期10卷 1934-1935页

作者： chuanbin yang Yuanyuan Cheng Jiao Zhao Jianhui Rong School of Chinese Medicine Li Ka Shing Faculty of MedicineUniversity of Hong Kong

Roles of Keap1-Nrf2 pathway in brain：Neuronal survival and neurogenesis are impaired in neurodegenerative diseases such as Parkinson＇s disease and Alzheimer＇s disease（Winner et al.,2011）.Genetic up-regulation of ... 详细信息

关键词： PACA Releasing Nrf2 to promote neurite outgrowth NGF

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Research on Facial Fatigue Detection of Drivers with Multi-feature Fusion

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Instrumentation 2023年第1期10卷 23-31页

作者： YE Yuxuan ZHOU Xianchun WANG Wenyan yang chuanbin ZOU Qingyu School of Electronic and Information Engineering Nanjing University of Information Science and TechnologyNanjing 210044 School of Artificial Intelligence(School of Future Technology) Nanjing University of Information Science and TechnologyNanjing 210044

In order to solve the shortcomings of current fatigue detection methods such as low accuracy or poor real-time performance,a fatigue detection method based on multi-feature fusion is ***,the HOG face detection algorithm and KCF target tracking algorithm are integrated and deformable convolutional neural network is introduced to identify the state of extracted eyes and mouth,fast track the detected faces and extract continuous and stable target faces for more efficient *** the head pose algorithm is introduced to detect the driver’s head in real time and obtain the driver’s head state ***,a multi-feature fusion fatigue detection method is proposed based on the state of the eyes,mouth and *** to the experimental results,the proposed method can detect the driver’s fatigue state in real time with high accuracy and good robustness compared with the current fatigue detection algorithms.

关键词： HOG Face Posture Detection Deformable Convolution Multi-feature Fusion Fatigue Detection

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