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International Journal of Minerals,Metallurgy and Materials 2015年第7期22卷 704-713页

作者： Wen-bin xin Bo Song chuan-gen Huang Ming-ming Song Gao-yang Song State Key Laboratory of Advanced Metallurgy University of Science and Technology Beijing School of Metallurgical and Ecological Engineering University of Science and Technology Beijing

The solidification microstructure, grain boundary segregation of soluble arsenic, and characteristics of arsenic-rich phases were systematically investigated in Fe-As alloys with different arsenic contents and quenching temperatures. The results show that the solidifica- tion microstructures of Fe-0.5wt%As alloys consist of irregular ferrite, while the solidification microstructures of Fe-4wt% As and Fe-10wt%As alloys present the typical dendritic morphology, which becomes finer with increasing arsenic content and quenching temperature. In Fe-0.5wt%As alloys quenched from 1600 and 1200℃, the grain boundary segregation of arsenic is detected by transmission electron microscopy. In Fe-4wt%As and Fe-10wt%As alloys quenched from 1600 and 1420℃, a fully divorced eutectic morphology is observed, and the eutectic Fe2As phase distributes discontinuously in the interdendritic regions. In contrast, the eutecfic morphology of Fe-10wt%As alloy quenched from 1200℃ is fibrous and forms a continuous network structure. Furthermore, the area fraction of the eutectic Fe2As phase in Fe-4wt%As and Fe-10wt%As alloys increases with increasing arsenic content and decreasing quenching temperature.

关键词： iron arsenic alloys solidification microstructure segregation eutectic

Generalized analysis model for fractional frequency reuse

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The Journal of China Universities of Posts and Telecommunications 2010年第5期17卷 1-5页

作者： XI Wei YIN Chang-chuan YUE Guang-xin SUN Hong-mei Laboratory of Wireless Cornmtmication Systems and Networks Beijing University of Posts and Telecommunications Beijing 100876 China Communication Technology Laboratory Intel China Research Center Beijing 100190 China

Fractional frequency reuse （FFR） is an effective technique to mitigate co-channel interference in orthogonal frequency division multiple access （OFDMA）-based broadband cellular *** this paper,we present a generalized model for FFR under which all existing FFR schemes can be considered as its special ***,quality factor has been proposed to indicate the quality of the *** interesting conclusion can be drawn that,as the power ratio in FFR is adjusted continuously,the corresponding quality factor varies ***,simulation is conducted based on worldwide interoperability for microwave access （WiMAX）,and the result agrees well with our theoretical ***,an effective range for power ratio is presented,which is very instructive to practical system design.

关键词： co-channel interference FFR power ratio

Methylation profile of bovine Oct4 gene coding region in relation to three germ layers

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Journal of Integrative Agriculture 2016年第3期15卷 618-628页

作者： ZHOU xin-yu LIU Liang-liang JIA Wen-chao PAN chuan-ying College of Life Sciences Northwest A&F University School of Computer Science & Engineering Jiangsu University of Science and Technology College of Animal Science and Technology Northwest A&F UniversityShaanxi Key Laboratory of Molecular Biology for Agriculture

Previous studies have shown that octamer-binding transcription factor 4（Oct4） plays a significant role in early embryonic development of mammalian animals, and different Oct4 expression levels induce multi-lineage differentiation which are regulated by DNA methylation. To explore the relationship between the methylation pattern of Oct4 gene exon 1 and embryonic development, in this work, five different tissues（heart, liver, lung, cerebrum and cerebellum） from three germ layers were chosen from low age（50–60 d） and advanced age（60–70 d） of fetal cattle and the differences between tissues or ages were analyzed, respectively. The result showed that the DNA methylation level of Oct4 gene exon 1 was significant different（P〈0.01） between any two of three germ layers in low age（〈60 d）, but kept steady of advanced age（P〉0.05）（〉60 d）, suggesting that 60-d post coital was an important boundary for embryonic development. In addition, in ectoderm（cerebrum and cerebellum）, there was no significant methylation difference of Oct4 gene exon 1 between low age and advanced age（P〉0.05）, but the result of endoderm（liver and lung） and mesoderm（heart） were on the contrary（P〈0.01）, which indicated the development of ectoderm was earlier than endoderm and mesoderm. The methylation differences from the 3rd, 5th and 9th Cp G-dinucleotide loci of Oct4 gene exon 1 were significantly different between each two of three germ layers（P〈0.05）, indicating that these three loci may have important influence on bovine embryonic development. This study showed that bovine germ layers differentiation was significantly related to the DNA methylation status of Oct4 gene exon 1. This work firstly identified the DNA methylation profile of bovine Oct4 gene exon 1 and its association with germ layers development in fetus and adult of cattle. Moreover, the work also provided epigenetic information for further studying bovine embryonic development and cellular reprogramming.

关键词： bovine DNA methylation octamer-binding transcription factor 4(Oct4) exon germ layer

Global Exponential Stability of a Class of Neural Networks with Delays

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Acta Mathematicae Applicatae Sinica 2009年第1期25卷 43-50页

作者： Feng-jian Yang Chao-long Zhang chuan-yong Chen xin-ming Chen Department of Computational Science Zhongkai University of Agriculture and Engineering Guangzhou 510225China

This paper is concerned with the stability of neural networks with time-varying delays. Under assumption that the nonlinear stimulate functions are Lipschitz continuous, by means of generalized Halanay inequalities, Dini＇s derivative and functional analysis techniques, several globally exponential stability criteria are established, which are only dependent on the parameters of the system.

关键词： Neural network Halanay's inequality delay globally exponential stability

Additivity of pore structural parameters of granular activated carbons derived from different coals and their blends

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International Journal of Mining Science and Technology 2016年第4期26卷 661-667页

作者： Yao xin Xie Qiang Yang chuan Zhang Bo Wan Chaoran Cui Shanshan School of Chemical and Environmental Engineering China University of Mining and Technology Department of Chemical and Food Engineering Hefei University of Technology

A series of granular activated carbons （GACs） were prepared by briquetting method from Chinese coals of different ranks and their blends, with coal pitch as the binder. Pore structural parameters including BET specific surface area （SBEr）, total pore volume （Vr） and average pore diameter （da） were measured and cal- culated as well as process parameters such as yield of char （CY） and burn-off （B）. The relationship between the pore structural parameters of the GAC from coal blend （BC-GAC） and the ones of the GACs from corresponding single coals （SC-GACs） was analyzed, in which an index, the relative error （δ）, was presented to define the bias between fitted values and experimental values of these parameters of the BC-GACs. The results show that the BC-GAC keeps qualitatively the pore structural features of the SC-GACs; as concerned as the quantitative relationship, the pore structural parameters of the BC-GAC from coal blend consisting of non-caking coals can be obtained by adding proportionally the pore structural parameters of the SC-GACs with a less than 10%. Meanwhile, for the BC-GAC from coal blend containing weak caking bituminous coal, the δ increases up to 25% and the experimental pore size distribution differs greatly from the fitted one.

关键词： Granular activated carbon Coal blend Pore structural parameters Additivity

Nested-PCR assay for detection of Schistosoma japonicum infection in domestic animals

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Infectious Diseases of Poverty 2017年第1期6卷 754-760页

作者： xin Zhang chuan-chuan he Jin-Ming Liu Hao Li Ke Lu Zhi-Qiang Fu chuan-Gang Zhu Yi-Ping Liu Lai-Bao Tong De-bao Zhou Li Zha Yang Hong Ya-Mei Jin Jiao-Jiao Lin Shanghai Veterinary Research Institute Chinese Academy of Agricultural SciencesKey Laboratory of Animal ParasitologyMinistry of AgricultureShanghai 200241People’s Republic of China Anhui Center for Animal Disease Control and Prevention HefeiPeople’s Republic of China Wangjiang county Center for Animal Husbandry and Veterinary Bureau AnqingPeople’s Republic of China Dongzhi county Center for Animal Husbandry and Veterinary Bureau ChizhouPeople’s Republic of China Huangshan Center for Animal Disease Control and Prevention Huangshan 341000People’s Republic of China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious and Zoonosea YangzhouJiangsu province 225009People’s Republic of China

Background:Schistosomiasis japonica is a common *** animals are the primary source of infection and play an important role in disease *** prevalence and infectivity of this disease in domestic animals in China have significantly decreased and,for this reason,diagnostics with a higher sensitivity have become increasingly *** was reported that polymerase chain reaction(PCR)-based methods could be used to detect schistosome infection in humans and animals and presented a high sensitivity and *** present study aimed to develop a PCR-based method for detection of Schistosoma japonicum infection in domestic ***:A specific nested-PCR assay was developed to detect *** infection in domestic animals via amplification of a 231-bp DNA fragment of retrotransposon *** developed assay was first used in sera and dry blood filter paper(DBFP)from goats and buffaloes at different time points of ***,78 DBFPs from 39 artificially-infected bovines at 14 and 28 days post-infection and 42 DBFPs from schistosome-negative bovines from the city of Huangshan in the Anhui province were used to evaluate the diagnostic ***,this assay was used to detect *** infection in domestic animals in Dongzhi and Wangjiang ***:The expected PCR product was detected in eggs and adult worms of *** and blood samples from ***-infected goats and water buffaloes,but not from Fasciola and Haemonchus contortus *** nested-PCR assay could detect the target *** DNA in DBFPs from goats and buffaloes after day 3 *** sensitivity in buffaloes at 14 and 28 days post-infection was 92.30%(36/39)and 100%(39/39),*** specificity was 97.60%(41/42).The positivity rates in Dongzhi and Wangjiang counties were 6.00%and 8.00%in bovines and 22.00%and 16.67%in goats,*** positivity rates in goats in both counties were higher than those in bovines wit

关键词： Schistosomiasis Domestic animals Nested-PCR Diagnosis

MdWRKY40is directly promotes anthocyanin accumulation and blocks MdMYB15L, the repressor of MdCBF2, which improves cold tolerance in apple

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Journal of Integrative Agriculture 2023年第6期22卷 1704-1719页

作者： XU Peng-yue XU Li XU Hai-feng he Xiao-wen he Ping CHANG Yuan-sheng WANG Sen ZheNG Wen-yan WANG chuan-zeng CheN xin LI Lin-guang WANG Hai-bo Shandong Institute of Pomology Shandong Academy of Agricultural SciencesTai’an 271000P.R.China Modern Agriculture Research Institute of Yellow River Delta Shandong Academy of Agricultural SciencesDongying 257000P.R.China

Cold stress is an important factor that limits apple production. In this study, we examined the tissue-cultured plantlets of apple rootstocks ‘M9T337' and ‘60-160', which are resistant and sensitive to cold stress, respectively. The enriched pathways of differentially expressed genes(DEGs) and physiological changes in ‘M9T337' and ‘60-160' plantlets were clearly different after cold stress(1°C) treatment for 48 h, suggesting that they have differential responses to cold stress. The differential expression of WRKY transcription factors in the two plantlets showed that MdWRKY40is and MdWRKY48 are potential regulators of cold tolerance. When we overexpressed MdWRKY40is and MdWRKY48in apple calli, the overexpression of MdWRKY48 had no significant effect on the callus, while MdWRKY40is overexpression promoted anthocyanin accumulation, increased callus cold tolerance, and promoted the expression of anthocyanin structural gene MdDFR and cold-signaling core gene MdCBF2. Yeast one-hybrid screening and electrophoretic mobility shift assays showed that MdWRKY40is could only bind to the MdDFR promoter. Yeast twohybrid screening and bimolecular fluorescence complementation showed that MdWRKY40is interacts with the CBF2inhibitor MdMYB15L through the leucine zipper(LZ). When the LZ of MdWRMY40is was knocked out, MdWRKY40is overexpression in the callus did not affect MdCBF2 expression or callus cold tolerance, indicating that MdWRKY40is acts in the cold signaling pathway by interacting with MdMYB15L. In summary, MdWRKY40is can directly bind to the MdDFR promoter in order to promote anthocyanin accumulation, and it can also interact with MdMYB15L to interfere with its inhibitory effect on MdCBF2, indirectly promoting MdCBF2 expression, and thereby improving cold *** results provide a new perspective for the cold-resistance mechanism of apple rootstocks and a molecular basis for the screening of cold-resistant rootstocks.

关键词： MdWRKY40is anthocyanin accumulation MdMYB15L MdCBF2 cold tolerance

RNA m^(6)A Modification in Cancers: Molecular Mechanisms and Potential Clinical Applications

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The Innovation 2020年第3期1卷 81-91页

作者： Chang Gu xin Shi Chenyang Dai Feng Shen Gaetano Rocco Jiafei Chen Zhengyu Huang Chunji Chen chuan he Tao Huang Chang Chen Department of Thoracic Surgery Shanghai Pulmonary HospitalTongji University School of MedicineShanghai 200433China Department of Cardiology Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghai 200030China School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai 200030China Thoracic Service Department of SurgeryMemorial Sloan Kettering Cancer CenterNew YorkNY 10065USA Druckenmiller Center for Lung Cancer Research Memorial Sloan Kettering Cancer CenterNew YorkNY 10065USA Department of Colorectal and Anal Surgery Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China Department of Thoracic Surgery Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghaiChina Department of Chemistry and Institute for Biophysical Dynamics The University of ChicagoChicagoILUSA Howard Hughes Medical Institute The University of ChicagoChicagoILUSA Medical Scientist Training Program/Committee on Cancer Biology The University of ChicagoChicagoILUSA Department of Biochemistry and Molecular Biology The University of ChicagoChicagoILUSA Shanghai Institute of Nutrition and health Chinese Academy of SciencesShanghaiChina

N^(6)-Methyladenosine(m^(6)A)RNA modification brings a new dawn for RNA modification researches in recent *** posttranscriptional RNA modification is dynamic and reversible,and is regulated by methylases(“writers”),demethylases(“erasers”),and proteins that preferentially recognize m^(6)A modifications(“readers”).The change of RNA m^(6)A modification regulates RNA metabolism in eucaryon,including translation,splicing,exporting,decay,and *** the dysregulation ofm^(6)A may lead to tumorigenesis and *** the tumorigenic role of abnormalm^(6)A expression,m^(6)A regulators may function as potential clinical therapeutic targets for *** this review,we emphasize on the underlying mechanisms of m^(6)A modifications in tumorigenesis and further introduce the potential m^(6)A regulators-associated therapeutic targets for tumor therapy.

关键词： N6-methyladenosine cancer RNA methylation therapy

LARGE EDDY SIMULATION OF OPEN CHANNEL FLOWS WITH NONSUBMERGED VEGETATION

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Journal of Hydrodynamics 2011年第2期23卷 258-264页

作者： HUAI Wen-xin WU Zhen-lei QIAN Zhong-dong GENG chuan State Key Laboratory of Water Resources and Hydropower Engineering Science Wuhan University Wuhan 430072 China

Results of several Large Eddy Simulations （LES） this article. It is shown that the vegetation can make the flow of open channel flows with non-submerged vegetation are presented in structure in the mainstream direction uniform for both supercritical and subcritical flows. For subcritical flows, the LES results of the ensemble-average of time-averaged velocity distributions at four vertical sections around a single plant are in good agreement with measurements. The velocity sees double peaks at the upper and lower positions of flows. For supercritical flows, the ensemble-average velocities see some discrepancy between LES and measurement results. Some secondary flow eddies appear near the single plant, and they just locate in the positions of the double peaks in stream-wise velocity profiles. It is also found that the vegetation drag coefficient deceases as the Froude number increases.

关键词： non-submerged sub/supercritical flow peak value Large Eddy Simulation (LES) secondary flow drag coefficient

Simultaneous Fe(Ⅲ) reduction and ammonia oxidation process in Anammox sludge

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Journal of Environmental Sciences 2018年第2期30卷 42-50页

作者： Xiang Li Yong Huang heng-wei Liu chuan Wu Wei Bi Yi Yuan xin Liu School of Environmental Science and Engineering Suzhou University of Science and Technology School of Chemistry Biology and Material Engineering Suzhou University of Science and Technology

In recent years, there have been a number of reports on the phenomenon in which ferric iron（Fe（Ⅲ）） is reduced to ferrous iron [Fe（Ⅱ）] in anaerobic environments, accompanied by simultaneous oxidation of ammonia to NO2-, NO3-, or ***, studies on the relevant reaction characteristics and mechanisms are rare. Recently, in research on the effect of Fe（Ⅲ） on the activity of Anammox sludge, excess ammonia oxidization has also been ***, in the present study, Fe（Ⅲ） was used to serve as the electron acceptor instead of NO2-,and the feasibility and characteristics of Anammox coupled to Fe（Ⅲ） reduction（termed Feammox） were investigated. After 160 days of cultivation, the conversion rate of ammonia in the reactor was above 80%, accompanied by the production of a large amount of NO3-and a small amount of NO2-. The total nitrogen removal rate was up to 71.8%. Furthermore,quantities of Fe（Ⅱ） were detected in the sludge fluorescence in situ hybridization（FISH） and denaturated gradient gel electrophoresis（DGGE） analyses further revealed that in the sludge, some Anammox bacteria were retained, and some microbes were enriched during the acclimatization process. We thus deduced that in Anammox sludge, Fe（Ⅲ） reduction takes place together with ammonia oxidation to NO2-and NO3-along with the Anammox process.

关键词： Anammox sludge Anammox coupled to Fe(Ⅲ) reduction Reaction characteristics Biological community analysis