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Insect Science 2013年第2期20卷 254-260页

作者： Qi-Yi Tang chuan-xi zhang Institute of Insect Science Zhejiang University Hangzhou China

A comprehensive but simple-to-use software package called DPS （Data Pro- cessing System） has been developed to execute a range of standard numerical analyses and operations used in experimental design, statistics and data mining. This program runs on standard Windows computers. Many of the functions are specific to entomological and other biological research and are not found in standard statistical sottware. This paper presents applications of DPS to experimental design, statistical analysis and data mining in entomology.

关键词： data mining DPS entomological research experimental design software,statistical analysis

FAR gene enables the brown planthopper to walk and jump on water in paddy field

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Science China(Life Sciences) 2019年第11期62卷 1521-1531页

作者： Dan-Ting Li Xuan Chen xin-Qiu Wang chuan-xi zhang State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology Institute of Insect ScienceZhejiang University

Many insects can live on water and survive being caught in the *** research has shown that insect cuticular hydrocarbons(CHC)confer desiccation resistance to maintain water *** this study,we identified a fatty acyl-CoA reductase gene(NlFAR)of the rice brown planthopper,Nilaparvata lugens that is essential for the production of CHCs,and found that NlFAR is essential for *** to walk and jump on water when moving from one rice plant to another in paddy *** was mainly expressed in the integument at the beginning of each *** surface analysis by scanning electron microscopy and characterization of CHC extracts indicated that *** with knockdown of NlFAR using RNA inference(RNAi)had a neater epicuticle layer and a significant decrease in CHC *** of NlFAR did not influence the desiccation resistance of ***,but the ds NlFAR-treated insects were easily adhered and moistened by water droplets or their own secreted honeydew and unable to walk or jump on *** results suggested that NlFAR is a crucial enzyme for CHC biosynthesis and cuticle waterproofing,but not for water retention of ***,which may provide a potential strategy for pest management.

关键词： Nilaparvata lugens fatty acyl-CoA reductase cuticular hydrocarbon waterproofing

Using chimeric piggyBac transposase to achieve directed interplasmid transposition in silkworm Bombyx mori and fruit fly Drosophila cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第9期11卷 728-734页

作者： Na WANG Cai-ying JIANG Ming-xing JIANG chuan-xi zhang Jia-an CHENG Institute of lnsect Sciences Zhejiang University Hangzhou 310029 China Institute of Biochemistry Zhejiang Sci-Tech University Hangzhou 310018 China

The piggyBac transposon has been long used to integrate foreign DNA into insect ***,undesirable transgene expression can result from random insertions into the *** this study,the efficiency of chimeric Gal4-piggyBac transposase in directing integration onto a DNA target plasmid was evaluated in cultured silkworm Bombyx mori Bm-12 and fruit fly Drosophila Schneider 2(S2) *** Gal4-piggyBac transposase has a Gal4 DNA-binding domain(DBD),and the target plasmid has upstream activating sequences(UAS) to which the Gal4 DBD can bind with high *** results indicate that,in the Bm-12 and S2 cells,transpositional activity of Gal4-piggyBac transposase was increased by 4.0 and 7.5 times,respectively,compared to controls,where Gal4-UAS interaction was ***,the Gal4-piggyBac transposase had the ability of directing piggyBac element integration to certain sites of the target plasmid,although the target-directing specificity was not as high as *** chimeric piggyBac transposase has the potential for use in site-directed transgenesis and gene function research in ***.

关键词： Bombyx mori piggyBac Gal4-upstream activating sequences(UAS) Transposition assay Transgenesis

Characteristics of the draft genome of ＂Candidatus Arsenophonus nilaparvatae＂, a facultative endosymbiont of Nilaparvata lugens

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Insect Science 2016年第3期23卷 478-486页

作者： Hai-Wei Fan Jia-Bao Lu Yu-Xuan Ye xiao-Ping Yu chuan-xi zhang Institute of Insect Science Zhejiang University College of Life Science China Jiliang University Hangzhou China

There exists a kind of symbiotic bacterium named ＂Candidatus Arsenophonus nilaparvatae＂ in the brown planthopper （BPH）, Nilaparvata lugens. After being filtered and assembled from the BPH genome sequencing project, the genome sequence of this bacterial symbiont was obtained. After initial analysis based on the genome, we have found its potential role to synthesize B vitamins for the host. In order to better understand the lifestyle and the genomic changes of this symbiotic bacterium after the symbiotic relationship was established, we further report the characteristics of this draft genome. Compared with several other related bacteria, ＂Candidatus Arsenophonus nilaparvatae＂ has proven to be a facultative endosymbiont at the genomic level. Concurrently, the presence of fimbriae and flagella formation related genes indicates this maternally transmitted endosymbiont is most likely to retain the capacity to invade new hosts. Through further analysis of annotated gene sets, we also find evidence of genome reduction in its secretion system and metabolic pathways. These findings reflect its evolutionary trend to be an obligate one and enable a deeper study of microbe-insect interactions.

关键词： endosymbiont evolution facultative genome Nilaparvata lugens

Identification of salivary proteins in the whitefly Bemisia tabaci by transcriptomic and LC-MS/MS analyses

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Insect Science 2021年第5期28卷 1369-1381页

作者： Hai-Jian Huang Zhuang-xin Ye Gang Lu chuan-xi zhang Jian-Ping Chen Jun-Min Li State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang ProvinceInstitute of Plant VirologyNingbo UniversityNingboZhejiang315211.China

The whitefly Bemisia tabaci is a notorious agricultural pest of many crops *** it is thought that *** secretes saliva into the host plant to counter plant defenses,knowledge on the whitefly salivary proteome is ***,we characterized the gene/protein repertoires of *** salivary glands and secreted saliva by transcriptomic and liquid chromatography tandem mass spectroscopy analyses.A total of 698 salivary gland-enriched unigenes and 171 salivary proteins were *** analysis between the *** salivary proteins and those of different arthropod species revealed numerous similarities in proteins associated with binding,hydrolysis,and oxidation-reduction,which demonstrates a degree of conservation across herbivorous *** were 74 proteins only identified in *** saliva,of which 34 were *** addition,13 salivary proteins,of which I1 were ***-specific,were differentially regulated when *** fed on different *** results provide a good resource for future functional studies of whitefly salivary effectors,and might be useful in pest management.

关键词： Bemisia tabaci proteome salivary protein species-specific transcriptome

Analysis of a late gene, orfl01 from Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus

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Insect Science 2005年第5期12卷 335-340页

作者： SHI-HENG AN LI-PING xiNG V. SHYAM KUMAR chuan-xi zhang Institute of Applied Entomology Zhejiang University Hangzhou College of Plant Protection Henan Agricultural University Zhengzhou China

Helicoverpa armigera single nucleocapsid nucleopolyhedrovirns open reading frame 101 （ha101） is 762 nts in length and encodes a 254 amino acid peptide with predicted 29 kDa molecular weight. The homologues of ha101 were explored using BLASTP searching tool in the updated GenBank/EMBL and SWlSS-PROT databases. The results showed that the homologues of ha101 were present in all the completely sequenced lepidopteran nucleopolyhedrovirnses and granulovirnses, suggesting that ha101 might be a functional gene associated with their lepidopteran hosts. Sequence alignment of ha101 and its homologues revealed that 10 amino acids were completely conserved. RT-PCR analysis of ha101 manifested that the transcript of ha101 was first detected at 24 hpi and remained detectable at up to 122 hpi, suggesting that ha101 was transcribed during late stages of infection. Ha101 was expressed using Bac to Bac system in Tn5B-1-4 cells. The product of ha101 expressed in Tn5B-1-4 cells was approximately 29kDa, consistent with the predicted molecular weight, and the results were confirmed by western blot analysis. The subcellular localization indicated that ha101 was aggregated along nuclear envelope during the early stages of infection and spread out to the entire nucleus including virogenic stroma in late stages of infection, suggesting that ha101 may play a specific role in virion assembly process or virogenic stroma arrangement.

关键词： expression ha101 Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus sequence analysis RT-PCR subcellular localization

Three-dimensional reconstruction of pore canals in the cuticle of the brown planthopper

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Science China(Life Sciences) 2021年第11期64卷 1992-1994页

作者： Dan-Ting Li Jian-Sheng Guo xin-Qiu Wang Bernard Moussian chuan-xi zhang Institute of Insect Science Zhejiang UniversityHangzhou 310058China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of Plant VirologyNingbo UniversityNingbo 315211China Department of Pathology of Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhou 310058China School of Medicine Zhejiang UniversityHangzhou 310058China UniversitéCôte d’Azur CNRSInsermInstitute of Biology ValroseParc Valrose06108 Nice CEDEX 2France

Dear Editor,The insect cuticle is a multifunctional coat that defines and stabilizes the shape of the body,appendages and internal organs,serves as a barrier against water and chemical compounds,such as insecticides,prevents infection and protects against predators(Moussian,2010).The surface of insects is covered by a lipid layer composed of diverse hydrocarbons,wax esters,fatty acids,fatty alcohols,sterols and *** of lipids to the body surface occurs through the cuticle by a nano-canal system consisting of pore canals(PC)and wax canals(WC).

关键词： alcohols hydrocarbons esters

Expression of two types of acetylcholinesterase gene from the silkworm, Bombyx mori, in insect cells

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Insect Science 2007年第6期14卷 443-449页

作者： JIN-YAN SHANG YA-MING SHAO GUO-JUN LANG GAN YUAN ZHEN-HUA TANG chuan-xi zhang Institute of Insect Sciences Zhejiang University Hangzhou Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai China

Complementary DNAs encoding two types of acetylcholinesterase （ACHE） were isolated from the silkworm, Bombyx mori. The type 1 （Bmacel） and type 2 （Bmace2） ORFs are 2052 and 1917 bp in length, respectively. Both the complete ORFs of the Bmaces and C- terminal truncated forms were recombined into the Bacmid baculovirus vector under the control of the polyhedrin promoter and expressed in Trichoplusia ni （Tn-5B 1-4） cells. The resulting products exhibited ACHE activity and glycosylation of the expressed proteins. An inhibition assay indicated that the ace2-type enzyme was more sensitive than the acel-type enzyme to inhibition by eserine and paraoxon.

关键词： acetylcholinesterase Bmacel Bmace2 characterization expression silkworm

A draft genome of the ghost moth, Thitarodes （Hepialus） sp., a medicinal caterpillar fungus

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Insect Science 2016年第2期23卷 326-329页

作者： Ruo-Lin Cheng Yong-xin Yu Ling-xiao Liu chuan-xi zhang Cheng-xiang Fang Institute of Insect Science Zhejiang University Hangzhou China Biological Process Science and Technology Co. Ltd. Chengdu China

Dear Editor, The Chinese caterpillar fungus, commonly known as ＂Dong Chong xia Cao＂（winter worm summer grass） in China, is one of the most valuable medicinal fungi in the world （zhang et al., 2012）. In the alpine meadows of the Tibetan Plateau （altitude 3500-5000 m）, the ascomycete Ophiocordyceps sinensis （syn. Cordyceps sinensis） par- asitizes the larvae of the ghost moth （Thitarodes spp.）, forming a fungus-caterpillar complex. The caterpillar fun- gus has long been used in traditional Chinese medicine （TCM） for hundreds of years, with a diverse list of phar- macological effects （Zhu et al., 1998）.

关键词： ghost moth Thitarodes (Hepialus) medicinal caterpillar fungus

Chitin synthase 1 and five cuticle protein genes are involved in serosal cuticle formation during early embryogenesis to enhance eggshells in Nilaparvata lugens

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Insect Science 2022年第2期29卷 363-378页

作者： Jia-Bao Lu Jian-Sheng Guo Xuan Chen Chen Cheng Xu-Mei Luo xiao-Ya zhang Bernard Moussian Jian-Ping Chen Jun-Min Li chuan-xi zhang State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang ProvinceInstitute of Plant VirologyNingbo UniversityNingboZhejiang ProvinceChina Institute of Insect Science Zhejiang UniversityHangzhouChina School of Medicine Zhejiang UniversityHangzhouChina UniversitéCôte d'Azur CNRSInsermInstitute of Biology ValroseNiceFrance

Many holo-and hemimetabolous insects enhance their eggshells during embryogenesis by forming a serosal cuticle(SC).To date,scholarly understanding of the SC composition and SC-related gene functions has been limited,especially for hemimetabolous *** this study,we initially performed transmission electron microscopic(TEM)observation and chitin staining of the SC in Nilaparvata lugens,a hemimetabolous rice pest known as the brown planthopper(BPH).We confirmed that the SC was a chitin-rich lamellar structure deposited gradually during the early *** RNA interference(RNAi)against Nilaparvata lugens chitin synthase 1(NlCHS1)in newly emerged and matured females resulted in decreases of egg hatchability by 100%and 76%,*** analyses revealed loss of the lamellar structure of the SC in dsNlCHS1-treated *** to temporal expression profiles,five cuticle protein coding genes,NlugCpr1/2/3/8/90,were specifically or highly expressed during the SC formation period,and NlugCpr1/2/3/90 were further detected in 72 h eggshells extract by ultra-performance liquid chromatography-tandem mass spectrometry/mass ***2/3/90 were likely three SC-specific cuticle *** observations of the SC following parental RNAi against NlugCpr1/2/3/8/90 demonstrated that NlugCpr3/8/90 were essential for SC *** study provided an understanding of the SC formation process and SC-related cuticle proteins in BPHs,which offer potential targets for pest control in the egg stage as well.

关键词： chitin synthase 1 cuticle protein eggshell embryogenesis Nilaparvata lugens serosal cuticle