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Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal genes and sex chromosomes in the diamondback moth,Plutella xylostella

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Journal of Integrative Agriculture 2024年第9期23卷 3089-3103页

作者： Shanyu Li Guifang Lin Haoqi wen Haiyan Lu Anyuan Yin Chanqin Zheng Feifei Li Qingxuan Qiao Lu Jiao Ling Lin Yi Yan Xiujuan Xiang Huang liao Huiting Feng Yussuf Mohamed Salum Minsheng You Wei Chen Weiyi He State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops/Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops/International Joint Research Laboratory of Ecological Pest Control Ministry of EducationInstitute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhou 350002China Ganzhou Key Laboratory of Greenhouse Vegetable Gannan Normal UniversityGanzhou 341000China

Genetic pest control strategies based on precise sex separation and only releasing sterile males can be accomplished by site-specific genome *** the current study,we showed that the mutation of single-allele Pxfl(2)d can significantly impair the normal mating behavior and testis development in male adults of the notorious cruciferous insect pest Plutella xylostella,in addition to its known functions in the ovarian development in female adults and egg *** CRISPR/Cas9-based knock-in experiments revealed that site-specific integration of an exogenous green fluorescent protein(GFP)gene into autosomal Pxfl(2)d for labelling mutants could be ***,this gene is not a suitable target for GFP insertion to establish a genetically stable knock-in strain because of the severe decline in reproductive *** further screened for the W-chromosome-linked and Z-chromosome-linked regions to test the knock-in efficiency mediated by CRISPR/*** results verified that both types of chromosomes can be targeted for the site-specific insertion of exogenous *** ultimately obtained a homozygous knock-in strain with the integration of both Cas9 and cyan fluorescent protein(CFP)expression cassettes on a Z-linked region in ***,which can also be used for early sex *** injecting the sgRNA targeting Pxfl(2)d alone into the eggs laid by female adults of the Z-Cas9-CFP strain,the gene editing efficiency reached 29.73%,confirming the success of expressing a functional Cas9 *** together,we demonstrated the feasibility of the knock-in of an exogenous gene to different genomic regions in ***,while the establishment of a heritable strain required the positioning of appropriate *** study provides an important working basis and technical support for further developing genetic strategies for insect pest control.

关键词： cruciferous specialist fl(2)d CRISPR/Cas9 knock-in sex chromosome

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基于注意力机制改进的无锚框舰船检测模型

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吉林大学学报（工学版） 2024年第5期54卷 1407-1416页

作者：高云龙任明吴川高文中国科学院长春光学精密机械与物理研究所长春130033

为提升模型对合成孔径雷达(SAR)图像多尺度舰船目标的检测能力,保证检测网络的实时性,提出一个基于注意力机制改进的无锚框舰船检测模型。在YOLOX网络特征金字塔处嵌入空洞注意力模块,调节感受野与多尺度融合的关系,强化特征的表示能力... 详细信息

为提升模型对合成孔径雷达(SAR)图像多尺度舰船目标的检测能力,保证检测网络的实时性,提出一个基于注意力机制改进的无锚框舰船检测模型。在YOLOX网络特征金字塔处嵌入空洞注意力模块,调节感受野与多尺度融合的关系,强化特征的表示能力。在检测头部设计中心性预测分支,对锚点的分类得分进行加权处理,调整模型的损失函数,优化检测结果。在数据集SSDD上进行的对比实验结果表明:本文提出的模型优于主流的深度网络检测模型,精度达到94.73%,且在检测精度和检测速度中取得最佳平衡。

关键词：计算机视觉舰船目标检测空洞卷积注意力机制无锚框

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Analysis of an adult diabetes mellitus caused by a rare mutation of the gene:A case report

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World Journal of Clinical Cases 2024年第19期12卷 3942-3949页

作者： wen-Xuan Li Li-Li Xu chuan-Feng Liu Bing-Zi Dong Yun-Yang Wang Department of Endocrine and Metabolic Diseases The Affiliated Hospital of Qingdao UniversityQingdao 266003Shandong ProvinceChina

BACKGROUND This study presents the clinical and genetic mutation characteristics of an unusual case of adult-onset diabetes mellitus occurring in adolescence,featuring a unique mutation in the peroxisome proliferator-activated receptor gamma(PPARG)*** Access Statement:Research data supporting this publication are available from the NN repository at ***/download/.CASE SUMMARY The methodology employed entailed meticulous collection of comprehensive clinical data from the probands and their respective family ***,high-throughput sequencing was conducted to analyze the PPARG genes of the patient,her siblings,and their *** results of this investigation revealed that the patient initially exhibited elevated blood glucose levels during pregnancy,accompanied by insulin resistance and ***,these strains displayed increased susceptibility to diabetic kidney disease without any discernible aggregation *** results from the gene detection process demonstrated a heterozygous mutation of guanine(G)at position 284 in the coding region of exon 2 of PPARG,which replaced the base adenine(A)(exon2c.284A>***95Cys).This missense mutation resulted in the substitution of tyrosine with cysteine at the 95th position of the translated ***,both of her siblings harbored a nucleotide heterozygous variation at the same site,and both were diagnosed with *** The PPARG gene mutation,particularly the ***95Cys mutation,may represent a newly identified subtype of maturity-onset diabetes of the *** subtype is characterized by insulin resistance and lipid metabolism disorders.

关键词： Diabetes Gene mutation Maturity-onset diabetes of the young Peroxisome proliferator-activated receptor gamma Lipid metabolism

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Risk factors for renal outcomes in children with antineutrophil cytoplasmic antibody-associated vasculitis:a nationwide retrospective study in China

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World Journal of Pediatrics 2024年第5期20卷 506-516页

作者： Li-wen Tan Jun-Li Wan Chun-Hua Zhu Hong Xu Zheng-Kun Xia Li-Zhi Chen Xiao-chuan Wu Fang Wang Xiao-Rong Liu Cheng-Guang Zhao Xiao-Zhong Li Jian-Hua Mao Xiao-wen Wang wen-Yan Huang Yu-Hong Li Jian-Jiang Zhang Shi-Pin Feng Jun Yang Jiao-Jiao Liu Chun-Lin Gao Li-Ping Rong Lan-Jun Shuai Ke Xu He-Jia Zhang Qiu Li Ai-Hua Zhang Mo Wang Department of Nephrology Children’s Hospital of Chongqing Medical UniversityZhongshan 2nd Rd.136Chongqing400014China Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and DisordersChina International Science and Technology Cooperation Base of Child Development and Critical DisordersChildren’s Hospital of Chongqing Medical UniversityChongqingChina Chongqing Key Laboratory of Pediatrics Children’s Hospital of Chongqing Medical UniversityChongqingChina Department of Nephrology Children’s Hospital of Nanjing Medical University72 Guangzhou RoadNanjing210008China Department of Nephrology Children’s Hospital of Fudan UniversityNational Paediatric Medical Center of ChinaShanghaiChina Department of Pediatrics Jinling HospitalNanjing Medical UniversityNanjingChina Department of Pediatrics Jinling HospitalThe First School of Clinical MedicineSouthern Medical UniversityNanjingChina Department of Pediatrics Jinling HospitalMedical School of Nanjing UniversityNanjingChina Department of Pediatric Nephrology and Rheumatology The First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhouChina Department of Pediatrics The Second Xiangya Hospital of Central South UniversityChangshaChina Department of Pediatrics Peking University First HospitalBeijingChina Department of Nephrology Beijing Children’s HospitalCapital Medical UniversityBeijing100045China National Center for Children’s Health BeijingChina Department of Pediatrics Shengjing Hospital of China Medical UniversityShenyangChina Department of Nephrology and Immunology Children’s Hospital of Soochow UniversitySuzhouChina Department of Nephrology Children HospitalZhejiang University School of MedicineHangzhouChina Department of Nephrology Wuhan Children’s Hospital(Wuhan Maternal and Child Healthcare Hospital)Tongji Medical CollegeHuazhong University of Science&TechnologyWuhanChina Department of Nephrology and Rheumatology Shanghai Children’s HospitalSchool

Background Pediatric antineutrophil cytoplasmic antibody-associated vasculitis(AAV)is a life-threatening systemic vasculitis featured by liability to renal ***,there are few studies on the risk factors and predictive models for renal outcomes of AAV in *** Data from 179 AAV children in multiple centers between January 2012 and March 2020 were collected *** risk factors and predictive model of end-stage renal disease(ESRD)in AAV were *** Renal involvement was the most typical manifestation(95.5%),and the crescent was the predominant pathological lesion(84.9%).The estimated glomerular filtration rate(eGFR)was evaluated in 114 patients,of whom 59.6%developed ESRD,and the median time to ESRD was 3.20 *** eGFR[P=0.006,odds ratio(OR)=0.955,95%confidence interval(CI)=0.924–0.987]and the percentages of global glomerulosclerosis(pGGS;P=0.018,OR=1.060,95%CI=1.010–1.112)were independent risk factors for ESRD of renal *** on the pGGS and eGFR at renal biopsy,we developed three risk grades of ESRD and one predictive *** Kaplan‒Meier curve indicated that renal outcomes were significantly different in different risk grades(P<0.001).Compared with serum creatinine at baseline,the predictive model had higher accuracy(0.86 versus 0.58,P<0.001)and a lower coefficient of variation(0.07 versus 0.92)in external *** Renal involvement is the most common manifestation of pediatric AAV in China,of which more than half deteriorates into *** predictive model based on eGFR at renal biopsy and the pGGS may be stable and accurate in speculating the risk of ESRD in AAV children.

关键词： Antineutrophil cytoplasmic antibody End-stage renal disease Glomerulonephritis Pediatric nephrology Vasculitis

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Simultaneous guidance of electromagnetic and elastic waves via glide symmetry phoxonic crystal waveguides

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Chinese Physics B 2024年第3期33卷 363-368页

作者：雷林霖何灵娟廖清华刘文兴于天宝 School of Physics and Materials Science Nanchang UniversityNanchang 330031China

A phoxonic crystal waveguide with the glide symmetry is designed,in which both electromagnetic and elastic waves can propagate along the glide plane at the same *** to the glide symmetry,the bands of the phoxonic crystal super-cell degenerate in pairs at the boundary of the Brillouin *** is the so-called band-sticking effect and it causes the appearance of gapless *** adjusting the magnitude of the glide dislocation the edge bandgaps,the bandgap of the guided-modes at the boundary of the Brillouin zone,can be further *** photonic and phononic guided-modes can then possess only one mode for a certain frequency with relatively low group velocities,achieving single-mode guided-bands with relatively flat dispersion *** addition,there exists acousto-optic interaction in the cavity constructed by the glide *** proposed waveguide has potential applications in the design of novel optomechanical devices.

关键词： phoxonic crystals glide symmetry waveguide acousto-optic interaction

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Medical imaging for the diagnosis,recurrence and metastasis evaluation of clear cell sarcoma

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World Journal of Clinical Cases 2024年第29期12卷 6258-6261页

作者： wen-Jing Wang Xia Wang Dong-Ming Hui Jun-Bang Feng chuan-Ming Li Department of Medical Imaging Chongqing University Central HospitalChongqing 400014China Department of Radiology Chongqing Western HospitalChongqing 400050China

Clear cell sarcoma(CCS)of soft tissue is extremely rare,accounting for approximately 1%of all soft tissue *** is very difficult to diagnose CCS based on clinical *** resonance imaging(MRI)provides highresolution images of soft tissues and pathological features such as mucus,necrosis,bleeding,and fat through high and low signals on T1 weighted image(T1WI)and T2 weighted image(T2WI).On the other hand,the paramagnetism of melanin in CCS shortens the relaxation time of T1 and T2,and high signal intensity on T1WI and low signal intensity on T2WI can be *** is different from most other soft tissue *** present,the treatment method for CCS is surgical *** can effectively display the tumour edge,extent of surrounding oedema,and extent of fat involvement,which is highly important for guiding surgical resection and predicting postoperative *** an invasive sarcoma,CCS has a high risk of *** of the pathological condition of the resected tumour,MRI or computed tomography(CT)should be performed every 1-2 years to assess recurrence at the primary site and to screen for metastasis in the lungs,liver,and *** necessary,PET-CT can be performed to evaluate the overall condition of the patient.

关键词： Clear cell sarcoma Recurrence Metastasis Computed tomography Magnetic resonance imaging Editorial

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国外先进产业体系构建经验与我国创新发展的探索路径

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科学管理研究 2024年第1期42卷 164-173页

作者：温惠淇张川东北大学马克思主义学院辽宁沈阳110169 东北大学工商管理学院辽宁沈阳110169

随着现代化产业经济的发展,创新发展现代化产业体系已成为各国经济发展战略的重要内容。推动经济发展、打造经济发展新引擎、促进产业结构升级、培育增长新动力,是我国在创新发展方面的自我体系建设的重要路径。通过对国外现代化产业体... 详细信息

随着现代化产业经济的发展,创新发展现代化产业体系已成为各国经济发展战略的重要内容。推动经济发展、打造经济发展新引擎、促进产业结构升级、培育增长新动力,是我国在创新发展方面的自我体系建设的重要路径。通过对国外现代化产业体系的发展历程和特点,发现国外现代化产业体系建设普遍把技术创新放在第一位,高度注重人才培养和创新环境建设;同时加强产学研深度合作,促进科技成果转化和应用,而且积极拓展国际市场,支持企业走出去开拓全球市场。目前我国现有产业体系不足,产业结构单一、技术创新不足和人才短缺问题严重,为此,进一步分析国内现代化产业体系的研究现状、发展特征及目前现代化产业体系发展路径,结合中国经济社会发展的具体实践,提出我国创新发展的路径,包括加强技术创新和人才培养、推动产业多元化发展、优化产业结构和提升国际竞争力,建设现代化产业体系高质量创新发展的对策建议。

关键词：国外产业体系构建经验创新发展探索研究

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AI for organic and polymer synthesis

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Science China Chemistry 2024年第8期67卷 2461-2496页

作者： Xin Hong Qi Yang Kuangbiao liao Jianfeng Pei Mao Chen Fanyang Mo Hua Lu wen-Bin Zhang Haisen Zhou Jiaxiao Chen Lebin Su Shuo-Qing Zhang Siyuan Liu Xu Huang Yi-Zhou Sun Yuxiang Wang Zexi Zhang Zhunzhun Yu Sanzhong Luo Xue-Feng Fu Shu-Li You Center of Chemistry for Frontier Technologies Department of ChemistryZhejiang UniversityHangzhou 310027China Center of Basic Molecular Science Department of ChemistryTsinghua UniversityBeijing 100084China Guangzhou National Laboratory Guangzhou 510005China Center for Quantitative Biology Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular ScienceFudan UniversityShanghai 200433China School of Materials Science and Engineering Peking UniversityBeijing 100871China Beijing National Laboratory for Molecular Sciences Center for Soft Matter Science and EngineeringKey Laboratory of Polymer Chemistry and Physics of Ministry of EducationCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China AI for Science(AI4S)-Preferred Program Shenzhen Graduate SchoolPeking UniversityShenzhen 518055China State Key Laboratory of Chemical Biology Shanghai Institute of Organic ChemistryUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200032China Department of Chemical Sciences National Natural Science Foundation of ChinaBeijing 100085China State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China

Recent years have witnessed the transformative impact from the integration of artificial intelligence with organic and polymer synthesis. This synergy offers innovative and intelligent solutions to a range of classic problems in synthetic chemistry. These exciting advancements include the prediction of molecular property, multi-step retrosynthetic pathway planning, elucidation of the structure-performance relationship of single-step transformation, establishment of the quantitative linkage between polymer structures and their functions, design and optimization of polymerization process, prediction of the structure and sequence of biological macromolecules, as well as automated and intelligent synthesis platforms. Chemists can now explore synthetic chemistry with unprecedented precision and efficiency, creating novel reactions, catalysts, and polymer materials under the datadriven paradigm. Despite these thrilling developments, the field of artificial intelligence(AI) synthetic chemistry is still in its infancy, facing challenges and limitations in terms of data openness, model interpretability, as well as software and hardware support. This review aims to provide an overview of the current progress, key challenges, and future development suggestions in the interdisciplinary field between AI and synthetic chemistry. It is hoped that this overview will offer readers a comprehensive understanding of this emerging field, inspiring and promoting further scientific research and development.

关键词： organic synthesis polymer synthesis machine learning prediction chemical database automated synthesis

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X-Paste improves wound healing in diabetes via NF-E2-related factor/HO-1 signaling pathway

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World Journal of Diabetes 2024年第6期15卷 1299-1316页

作者： Ming-Wei Du Xin-Lin Zhu Dong-Xing Zhang Xian-Zhen Chen Li-Hua Yang Jin-Zhou Xiao wen-Jie Fang Xiao-Chun Xue Wei-Hua Pan Wan-Qing liao Tao Yang Institute of Cardiovascular Disease Shanghai Key Laboratory of Traditional Chinese Clinical MedicineShanghai University of Traditional Chinese MedicineShanghai 200120China Department of Dermatology Shanghai Key Laboratory of Molecular Medical MycologySecond Affiliated Hospital of Naval Medical UniversityShanghai 200003China Department of Dermatology Dongshan HospitalMeizhou 514000Guangdong ProvinceChina Department of Dermatology Central Hospital Affiliated to Shandong First Medical UniversityJinan 250000Shandong ProvinceChina Department of Emergency Naval Hospital of Eastern TheaterZhoushan 316000Zhejiang ProvinceChina Department of Pharmacy 905th Hospital of People’s Liberation Army of China(PLA)NavyShanghai 200052China

BACKGROUND Diabetic foot ulcers(DFU),as severe complications of diabetes mellitus(DM),significantly compromise patient health and carry risks of amputation and *** To offer new insights into the occurrence and development of DFU,focusing on the therapeutic mechanisms of X-Paste(XP)of wound healing in diabetic *** Employing traditional Chinese medicine ointment preparation methods,XP combines various medicinal ***-performance liquid chromatography(HPLC)identified XP’s main *** streptozotocin(STZ)-induced diabetic,we aimed to investigate whether XP participated in the process of diabetic wound ***-sequencing analyzed gene expression differences between XP-treated and control *** docking clarified XP’s treatment mechanisms for diabetic wound *** umbilical vein endothelial cells(HUVECs)were used to investigate the effects of Andrographolide(Andro)on cell viability,reactive oxygen species generation,apoptosis,proliferation,and metastasis in vitro following exposure to high glucose(HG),while NF-E2-related factor-2(Nrf2)knockdown elucidated Andro’s molecular *** XP notably enhanced wound healing in mice,expediting the healing ***-sequencing revealed Nrf2 upregulation in DM tissues following XP *** identified 21 primary XP components,with Andro exhibiting strong Nrf2 *** mitigated HG-induced HUVECs proliferation,metastasis,angiogenic injury,and inflammation *** alleviates HG-induced HUVECs damage through Nrf2/HO-1 pathway activation,with Nrf2 knockdown reducing Andro’s proliferative and endothelial protective *** XP significantly promotes wound healing in STZ-induced diabetic *** XP’s key component,Andro activates the Nrf2/HO-1 signaling pathway,enhancing cell proliferation,tubule formation,and inflammation reduction.

关键词： Words:Diabetes mellitus Wound healing NF-E2-related factor-2/HO-1 signaling pathway Andrographolide

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Comprehensive assessment of the association between tumorinfiltrating immune cells and the prognosis of renal cell carcinoma

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World Journal of Clinical Oncology 2024年第10期15卷 1280-1292页

作者： Guo-Hao Wei Xi-Yi Wei Ling-Yao Fan wen-Zheng Zhou Ming Sun chuan-Dong Zhu Department of Oncology The Second Hospital of NanjingAffiliated to Nanjing University of Chinese MedicineNanjing 210003Jiangsu ProvinceChina The State Key Laboratory of Reproductive Department of UrologyThe First Affiliated Hospital of Nanjing Medical UniversityNanjing 210003Jiangsu ProvinceChina

BACKGROUND According to current statistics,renal cancer accounts for 3%of all cancers *** cell carcinoma(RCC)is the most common solid lesion in the kidney and accounts for approximately 90%of all renal *** evi-dence has shown an association between immune infiltration in RCC and clinical *** discover possible targets for the immune system,we investigated the link between tumor-infiltrating immune cells(TIICs)and the prognosis of *** To investigate the effects of 22 TIICs on the prognosis of RCC patients and iden-tify potential therapeutic targets for RCC *** The CIBERSORT algorithm partitioned the 22 TIICs from the Cancer Genome Atlas cohort into *** regression analysis was employed to evaluate the impact of 22 TIICs on the probability of developing RCC.A predictive model for immunological risk was developed by analyzing the statistical relationship between the subpopulations of TIICs and survival ***,multi-variate Cox regression analysis was used to investigate independent factors for the prognostic prediction of RCC.A value of P<0.05 was regarded as statistically *** Compared to normal tissues,RCC tissues exhibited a distinct infiltration of im-mune *** immune risk score model was established and univariate Cox regression analysis revealed a significant association between four immune cell types and the survival risk connected to ***-risk individuals were correlated to poorer outcomes according to the Kaplan-Meier survival curve(P=1E-05).The immunological risk score model was demonstrated to be a dependable predictor of survival risk(area under the curve=0.747)via the receiver operating characteristic *** to multivariate Cox regression analysis,the immune risk score model independently predicted RCC patients'prognosis(hazard ratio=1.550,95%CI:1.342–1.791;P<0.001).Finally,we established a nomogram that accurately and comprehensively fore

关键词： Renal cell carcinoma Tumor-infiltrating immune cells Prognosis Immune risk score model Nomogram

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