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Isolation of a feline-derived feline panleukopenia virus with an A300P substitution in the VP2 protein and confirmation of its pathogenicity in dogs

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动物疾病（英文） 2024年第3期4卷 173-185页

作者： Jiakang Li Jiajia Peng Yue Zeng Ying Wang Luying Li Yiran Cao Longlong Cao QingXiu Chen Zijun Ye Dengyuan zhou Shengbo Cao Qiuyan Li National Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan 430070HubeiChina Laboratory of Animal Virology Col-lege of Veterinary MedicineHuazhong Agricultural UniversityWuhanHubeiP.R.China Wuhan Keqian Biology Co. LtdWuhanHubeiP.R.China Depart-ment of Life Science Imperial College LondonLondonUK National Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan 430070HubeiChina Laboratory of Animal Virology Col-lege of Veterinary MedicineHuazhong Agricultural UniversityWuhanHubeiP.R.China Wuhan Keqian Biology Co. LtdWuhanHubeiP.R.China

Feline panleukopenia virus(FPV)is a single-stranded DNA virus that can infect cats and cause feline panleukope-nia,which is a highly contagious and fatal disease in *** sequence of FPV is highly variable,and mutations in the amino acids of its capsid protein play crucial roles in altering viral virulence,immunogenicity,host selection,and other *** this study,the epidemiology of FPV was studied using 746 gastrointestinal swab samples derived from cats that presented gastrointestinal symptoms specifically,diarrhea or vomiting during the period spanning from 2018 to *** overall prevalence of FPV-positive patients among these samples was determined to be 45.4％.Capsid(virion)protein 2(VP2)gene of each FPV-positive sample was sequenced and amplified,yielding 65 VP2 *** them,six VP2 gene sequences were detected in the majority of the samples test positive for FPV,and these positive samples originated from a diverse range of geographical *** isolates were named FPV-6,FPV-10,FPV-15,FPV-251,FPV-271 and ***,the substitution of Ala300Pro(A300P)in VP2 was detected for the first time in feline-derived FPV(FPV-251).FPV-251 isolate,with this substitution in VP2 protein,exhibited stable proliferative capacity in Madin-Darby canine kidney(MDCK)cells and A72 ***-271 was selected as the FPV control isolate due to its single amino acid difference from VP2 protein of FPV-251 at position 300(FPV-271 has alanine,while FPV-251 has proline).After oral infection,both FPV-251 and FPV-271 isolates caused feline panleu-kopenia,which is characterized by clinical signs of ***,FPV-251 can infect dogs through the oral route and cause gastrointestinal(GI)symptoms with lesions in the intestine and mesenteric lymph nodes(MLNs)of infected *** is the first report on the presence of an A300P substitution in VP2 protein of feline-derived ***,FPV isolate with a substitution of A300P at VP2 protein demons

关键词： Feline panleukopenia virus FPV Dogs VP2 gene characteristic Host range

自主跑步运动对APP/PS1小鼠内侧前额叶皮质少突胶质细胞的作用

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重庆医科大学学报 2024年第8期49卷 967-977页

作者：熊瑶周春妮蒋林张钐钐朱琳郭一静周宇宁黄杜娟李静祝佩林刘梅唐勇重庆医科大学基础医学院组织学与胚胎教研室重庆400016 重庆医科大学实验教学管理中心重庆400016 陆军军医大学第二附属医院麻醉科重庆400037 北京老年医院老年健康与医养结合研究室北京100095 日照市中医医院病理科日照276800 重庆市永川区妇幼保健院妇产科重庆402160

目的:研究自主跑步运动对APP/PS1双转基因AD(Alzheimer’s disease,AD)模型小鼠内侧前额叶皮质(medial prefrontal cortex,mPFC)内少突胶质细胞数量,少突胶质细胞分化、成熟以及髓鞘形成的作用。方法:将10月龄雄性转基因AD模型小鼠随机... 详细信息

目的:研究自主跑步运动对APP/PS1双转基因AD(Alzheimer’s disease,AD)模型小鼠内侧前额叶皮质(medial prefrontal cortex,mPFC)内少突胶质细胞数量,少突胶质细胞分化、成熟以及髓鞘形成的作用。方法:将10月龄雄性转基因AD模型小鼠随机分为AD组和AD跑步(AD+RUN)组,另外将同月龄同窝生野生型小鼠随机分为正常(WT)组和正常跑步(WT+RUN)组。其中跑步组于跑步笼内给予3个月自主跑步锻炼,AD组和WT组不做处理。跑步锻炼结束后4组小鼠同时给予行为学测试,用Morris水迷宫检测小鼠空间学习、记忆能力;用Y迷宫检测小鼠工作记忆能力;用新物体识别实验检测小鼠的认知记忆功能;利用无偏体视学技术和免疫组化技术相结合精确定量小鼠大脑mPFC内成熟少突胶质细胞(CC1^(+))总数量;同时分析小鼠mPFC内边缘皮层、Ⅱ、Ⅲ、Ⅵ、Ⅵ层成熟少突胶质细胞密度;利用免疫荧光和激光共聚焦显微镜分析小鼠mPFC内未成熟少突胶质细胞(PDGFα^(+)/Olig2^(+))密度、髓鞘标志物髓鞘碱性蛋白(myelin basic protein,MBP)荧光强度。结果:AD+RUN组和WT组在Morris水迷宫、Y迷宫以及新物体识别实验中的表现优于AD组,AD组mPFC内MBP荧光强度明显低于WT组(P<0.05),而AD+RUN组mPFC内MBP荧光强度明显大于AD组(P<0.001)。AD+RUN组mPFC内CC1^(+)细胞总数较AD组明显增加(P<0.05)。AD+RUN组mPFC内第Ⅲ层和第Ⅴ层CC1^(+)细胞密度较AD组明显增高(P<0.05),AD组mPFC内第Ⅴ层和第Ⅵ层CC1^(+)细胞密度较WT组明显降低(P<0.05)。AD组mPFC内PDGFα^(+)/Olig2^(+)细胞密度明显大于WT组,而AD+RUN组mPFC内PDGFα^(+)/Olig2^(+)细胞密度明显小于AD组(P<0.05)。结论:自主跑步运动能促进AD小鼠少突胶质细胞分化和髓鞘形成,这可能与运动能改善APP/PS1转基因AD小鼠的学习记忆能力有关。

关键词：阿尔茨海默病自主跑步运动内侧前额叶皮质少突胶质细胞髓鞘

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基于实际预压载作业的自升式平台环境载荷图谱绘制新思路

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哈尔滨工程大学学报（英文版） 2024年第1期23卷 209-221页

作者：孙承猛林海花张志刚孙洪源周立师 Naval Architecture and Port Engineering College Shandong Jiao Tong UniversityWeihai 264209China School of Ocean Engineering Harbin Institute of TechnologyWeihai 264209China China Merchants Jinling Shipyard(Weihai)Co. Ltd.Weihai 264207China

The environmental load chart is an important technical support required for the jack-up drilling platform to facilitate its adaptation to different operating waters and ensure the safety of *** chart is a crucial part of the platform operation *** chart data are closely related to external factors such as water depth,wind,wave,and current conditions of the working water,as well as to the structural characteristics of the platform itself and the number of variable *** study examines the platform state under extreme wind,wave,and current conditions during *** addition,this study focuses on the difference between the ultimate reaction force of the pile leg during preloading and the reaction force of the pile leg without considering any environmental load before ***,the relationship between the difference and the new reaction force of the pile leg caused by the combination of different environmental conditions is established to facilitate the construction of a new form of environmental load *** newly formed chart is flexible and simple;thus,it can be used to evaluate the environmental adaptability of the platform in the target well location and provides the preloading target demand or variable load limit according to the given environmental ***,the platform can perform personalized preloading operations,thereby improving its capability to cope with complex geological conditions,such as reducing punch-through *** condition reduces the load on jacking system devices and increases its service life.

关键词： Jack-up Environmental load chart Preloading Geometrically nonlinear effect Punch through

RepViTS-YOLOX:水下模糊及遮挡目标检测方法

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计算机工程与应用 2024年第13期60卷 200-208页

作者：陶洋朱腾钟邦乾周昆周立群重庆邮电大学通信与信息工程学院重庆400065

针对水下目标检测中的目标模糊和遮挡问题,提出基于YOLOX改进的RepViTS-YOLOX水下目标检测方法。该方法采用RepViTS作为特征提取网络并通过结构重参数化,有效提升了对水下目标的特征提取能力和模型推理速度。引入空间和通道重构(spatial... 详细信息

针对水下目标检测中的目标模糊和遮挡问题,提出基于YOLOX改进的RepViTS-YOLOX水下目标检测方法。该方法采用RepViTS作为特征提取网络并通过结构重参数化,有效提升了对水下目标的特征提取能力和模型推理速度。引入空间和通道重构(spatial and channel reconstruction convolution,SCConv)模块,增强网络对模糊目标的关注。改进特征融合网络,通过跨尺度连接和多尺度融合,加强不同层次特征间的信息交流,使模型更好理解遮挡目标特征。针对定位和分类任务对特征的不同需求,引入上下文解耦头(task-specific context decoupling head,TSCODE),对遮挡目标更精准地定位和分类。实验结果证明,RepViTS-YOLOX方法在RUOD数据集上取得了85.7%的检测效果,较YOLOX提高了3.8个百分点。检测结果显示,该方法可以有效改善水下模糊和遮挡目标的检测情况,提高水下目标检测精度。

关键词： YOLOX 目标检测结构重参数化解耦检测头注意力机制

Quantum effects on thermal vibration of single-walled carbon nanotubes conveying fluid

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Acta Mechanica Solida Sinica 2017年第5期30卷 550-556页

作者： Ye-Wei Zhang lin zhou Bo Fang Tian-Zhi Yang Department of Astronautics Shenyang Aerospace University Shenyang 110136 PR China Department of Mechanics Tianjin University Tianjin 300072 PR China

In this paper, we investigate the microfluid induced vibration of a nanotube in thermal environment. Attention is focused on a special case that the law of energy equipartition is unreliable unless the quantum effect is taken into account. A nonlocal luler-Bernoulli beam model is used to model the transverse vibration of a single-walled nanotube （SWCNT）. Results reveal that the root of mean squared （RMS） amplitude of thermal vibration of the fluid-conveying SWCNT predicted from the quantum theory is lower than that predicted from the law of energy equipartition. The quantum effect on the thermal vibration of the fluid-conveying SWCNT is more significant for the cases of higher-order modes, lower flow velocity, lower temperature, and lower fluid density.

关键词： Quantum effectsLaw of energy equipartitionFluid-conveying SWCNTEuler beam theoryRoot of mean-squared amplitude

人血浆中尼洛替尼的LC-MS/MS测定法及其药动学应用

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中国新药杂志 2024年第1期33卷 62-67页

作者：马欢周臻李周葛庆华康修远医药先进制造国家工程研究中心上海201203

目的:建立LC-MS/MS法测定人血浆中的尼洛替尼浓度,并应用于尼洛替尼胶囊中国健康受试者空腹药动学研究。方法:以氘代尼洛替尼为内标,血浆样品采用甲醇沉淀的方式分离蛋白并离心取上清液进样。色谱柱为Kinetex C_(18)柱(100 mm×2.1 mm,... 详细信息

目的:建立LC-MS/MS法测定人血浆中的尼洛替尼浓度,并应用于尼洛替尼胶囊中国健康受试者空腹药动学研究。方法:以氘代尼洛替尼为内标,血浆样品采用甲醇沉淀的方式分离蛋白并离心取上清液进样。色谱柱为Kinetex C_(18)柱(100 mm×2.1 mm,2.6μm)。流动相A为水(含0.1%甲酸和5 mmol·L^(-1)甲酸铵),流动相B为甲醇,67%B进样,流速为0.4 mL·min^(-1)。尼洛替尼及内标的离子对分别为530.25→289.30和536.25→295.30。方法经完整验证后,应用于12例中国健康受试者空腹药动学研究。结果:血浆中尼洛替尼在2~600 ng·mL^(-1)范围内线性关系良好,方法回收率为97.0%~104.3%,批内RSD≤4.18%,批间RSD≤6.43%。正常血浆无明显基质效应,分别在正常血浆中添加5%全血和2%脂肪乳剂考察特殊基质效应,证明溶血和高脂基质不影响测定准确性。12例中国健康受试者口服尼洛替尼胶囊后,经统计t_(1/2)为(24.7±32.2)h,C_(max)为(400.1±164.6)ng·mL^(-1),T_(max)为(2.4±0.9)h,AUC_(0→72)为(7900±2598)ng·mL^(-1)·h,AUC_(0→∞)为(9226±3649)ng·mL^(-1)·h。结论:本方法快速灵敏、准确度高、准确性好,适用于尼洛替尼的血药浓度测定及药动学研究。

关键词：尼洛替尼 LC-MS/MS法方法验证药动学

Notoginsenoside Ft1 inhibits colorectal cancer growth by increasing CD8+ T cell proportion in tumor-bearing mice through the USP9X signaling pathway

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Chinese Journal of Natural Medicines 2024年第4期 329-340页

作者： FENG Yutao LI Yuan MA Fen WU Enjiang CHENG Zewei zhou Shiling WANG Zhengtao YANG Li SUN Xun ZHANG Jiwei Shanghai Key Laboratory of Compound Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine Gastrointestinal surgery Longhua Hospital Shanghai University of Traditional Chinese Medicine

The management of colorectal cancer（CRC） poses a significant challenge, necessitating the development of innovative and effective therapeutics. Our research has shown that notoginsenoside Ft1（Ng-Ft1）, a small molecule, markedly inhibits subcutaneous tumor formation in CRC and enhances the proportion of CD8+T cells in tumor-bearing mice, thus restraining tumor *** into the mechanism revealed that Ng-Ft1 selectively targets the deubiquitination enzyme USP9X, undermining its role in shielding β-catenin. This leads to a reduction in the expression of downstream effectors in the Wnt signaling pathway. These findings indicate that Ng-Ft1 could be a promising small-molecule treatment for CRC, working by blocking tumor progression via the Wnt signaling pathway and augmenting CD8+T cell prevalence within the tumor environment.

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合肥红外自由电子激光光束线的设计与性能

中国激光 2024年第8期51卷 283-291页

作者：高琛鲍骏周银贵杨远俊孙松朱晓娣李和廷张善才王琳中国科学技术大学国家同步辐射实验室安徽合肥230029 中国科学院大学物理科学学院北京101408 合肥工业大学物理学院安徽合肥230009 安徽大学化学化工学院安徽合肥230601

红外光谱有着广泛的应用。合肥红外自由电子激光装置能够为用户提供高亮度的中/远红外辐射,为高水平的红外研究提供基础条件。自由电子激光和实验站之间需要用光束线连接起来,以便在完成红外辐射高效输送的同时进行聚焦、诊断等。本文... 详细信息

红外光谱有着广泛的应用。合肥红外自由电子激光装置能够为用户提供高亮度的中/远红外辐射,为高水平的红外研究提供基础条件。自由电子激光和实验站之间需要用光束线连接起来,以便在完成红外辐射高效输送的同时进行聚焦、诊断等。本文介绍了合肥红外自由电子激光装置红外光束线的设计与性能,主要包括光束线的总体要求、设计方案和布局、光学设计、光斑演化、光束传输、激光的分束取样、激光宏脉冲的在线同步测量、激光光谱的在线同步测量等。调试结果表明,设计达到了预期指标,整个光束线可以稳定运行。

关键词：激光光学光束线自由电子激光中/远红外激光诊断

臀大肌肌瓣联合筋膜脂肪瓣修复坐骨结节Ⅲ、Ⅳ期压力性损伤的疗效观察

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创伤外科杂志 2024年第2期26卷 120-125页

作者：唐林肖洪益小平周鑫陆军军医大学第一附属医院江北院区(陆军第九五八医院)骨科重庆400020 陆军军医大学新桥医院整形美容科重庆400037

目的探讨臀大肌肌瓣联合筋膜脂肪瓣修复坐骨结节Ⅲ、Ⅳ期压力性损伤的疗效。方法回顾性分析2018年6月—2020年9月陆军九五八医院骨科收治坐骨结节压力性损伤患者15例,男性10例,女性5例;年龄42~75岁,平均63.7岁;病灶累及单侧者12例、双侧... 详细信息

目的探讨臀大肌肌瓣联合筋膜脂肪瓣修复坐骨结节Ⅲ、Ⅳ期压力性损伤的疗效。方法回顾性分析2018年6月—2020年9月陆军九五八医院骨科收治坐骨结节压力性损伤患者15例,男性10例,女性5例;年龄42~75岁,平均63.7岁;病灶累及单侧者12例、双侧者3例。原发疾病为脊髓损伤截瘫者8例,脑血管意外后遗症者4例,骨盆骨折长期卧床者3例。根据2016年修订版国际压疮指南分期,Ⅲ期创面5例、Ⅳ期创面10例。入院时创面面积2.0 cm×5.0 cm~8.5 cm×11.0 cm。一期彻底清创将慢性感染病灶转变为无菌新鲜创面,转移臀大肌肌瓣填塞空腔,设计筋膜脂肪瓣覆盖创口,术后观察切口愈合情况,随访患者压疮复发情况、臀部外观及肌瓣供区继发功能障碍和畸形情况等。结果13例患者切口Ⅰ级愈合,2例患者创面因粪便污染经二期换药后愈合。患者均获16~24个月随访,平均20.3个月。未见压疮复发,患处皮肤质地良好、外形饱满、轻度色素沉着、无窦道形成,供区无继发功能障碍或畸形。结论联合臀大肌肌瓣和筋膜脂肪瓣修复坐骨结节Ⅲ~Ⅳ期压力性损伤的临床效果较佳,术后未见压疮复发,患处皮肤质地和外形良好,供区无继发功能障碍或畸形。

关键词：压力性损伤臀大肌肌瓣筋膜脂肪瓣坐骨结节