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Mucosal immune responses to Ichthyophthirius multifiliis in the ocular mucosa of rainbow trout(Oncorhynchus mykiss,Walbaum),an ancient teleost fish

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Marine Life Science & Technology 2024年第2期6卷 266-279页

作者： Weiguang Kong Guangyi Ding Gaofeng cheng peng Yang Zhen Xu Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China Department of Aquatic Animal Medicine College of FisheriesHuazhong Agricultural UniversityWuhan 430070China

The eye,as a specialized visual organ,is directly exposed to the external environment,and,therefore,it faces constant chal-lenges from external pathogenic organisms and *** the ocular mucosa(OM)of mammals,mucosal-associated lymphoid tissues(MALTs)constitute the primary line of ***,the immune defense role of the OM remains unknown in aquatic *** gain insights into the immune processes within the OM of teleost fish,we developed an infection model of rainbow trout(Oncorhynchus mykiss)OM using a parasite,Ichthyophthirius multifiliis(Ich).Immunofluorescence,qPCR,and H&E staining revealed that Ich successfully infiltrates the OM of rainbow trout,leading to pathological struc-tural changes,as evidenced by A&B ***,the qPCR results indicate an up-regulation of immune-related genes following Ich infection in the ***,transcriptome analyses were conducted to detect immune responses and impairments in eye function within the OM of rainbow trout with Ich *** results of the transcriptome analysis that Ich infection can cause an extensive immune response in the OM,ultimately affecting ocular *** the best of our knowledge,our findings represent for the first time that the teleost OM could act as an invasion site for parasites and trigger a strong mucosal immune response to parasitic infection.

关键词： Ocular mucosa Mucosal immunity Transcriptome analysis Oncorhynchus mykiss Ichthyophthirius multifiliis

Structured Multi-Head Attention Stock Index Prediction Method Based Adaptive Public Opinion Sentiment Vector

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Computers, Materials & Continua 2024年第1期78卷 1503-1523页

作者： cheng Zhao Zhe peng Xuefeng Lan Yuefeng Cen Zuxin Wang School of Economics Zhejiang University of TechnologyHangzhouChina College of Management Zhejiang University of TechnologyHangzhouChina Informatization Office Zhejiang University of TechnologyHangzhouChina School of Information and Electronic Engineering Zhejiang University of Science&TechnologyHangzhouChina School of Economics and Management Universiti Putra MalaysiaDaerah PetalingNegeri SelangorMalaysia

The present study examines the impact of short-term public opinion sentiment on the secondary market,with a focus on the potential for such sentiment to cause dramatic stock price fluctuations and increase investment *** quantification of investment sentiment indicators and the persistent analysis of their impact has been a complex and significant area of *** this paper,a structured multi-head attention stock index prediction method based adaptive public opinion sentiment vector is *** proposedmethod utilizes an innovative approach to transform numerous investor comments on social platforms over time into public opinion sentiment vectors expressing complex *** then analyzes the continuous impact of these vectors on the market through the use of aggregating techniques and public opinion data via a structured multi-head attention *** experimental results demonstrate that the public opinion sentiment vector can provide more comprehensive feedback on market sentiment than traditional sentiment polarity ***,the multi-head attention mechanism is shown to improve prediction accuracy through attention convergence on each type of input information *** absolute percentage error(MAPE)of the proposedmethod is 0.463%,a reduction of 0.294% compared to the benchmark attention ***,the market backtesting results indicate that the return was 24.560%,an improvement of 8.202% compared to the benchmark *** results suggest that themarket trading strategy based on thismethod has the potential to improve trading profits.

关键词： Public opinion sentiment structured multi-head attention stock index prediction deep learning

Controllable Exciton Diffusion Length and Ultrafast Charge Generation in Ternary Organic Solar Cells

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Chinese Journal of Chemistry 2024年第11期42卷 1275-1283页

作者： Sixuan cheng Jiawei Qiao peng Lu Wei Qin Xiaotao Hao School of Physics State Key Laboratory of Crystal MaterialsShandong UniversityJinanShandong250100 China School of Physics National Demonstration Center for Experimental Physics EducationShandong UniversityJinanShandong250100 China ARC Centre of Excellence in Exciton Science School of ChemistryThe University of MelbourneParkvilleVictoria3010 Australia

Charge generation,a critical process in the operation of organic solar cell(OSC),requires thorough investigation in an ultrafast *** work demonstrates that the utilization of alloy model for the non-fullerene acceptor(NFA)component can regulate the crystallization properties of active layer films,which in turn affects exciton diffusion and hole transfer(HT),ultimately influencing the charge generation *** incorporating BTP-eC7 as a third component,without expanding absorption range or changing molecular energy levels but regulating the ultrafast exciton diffusion and HT processes,the power conversion efficiency(PCE)of the optimized PM6:BTP-eC9:BTP-eC7 based ternary OSC is improved from 17.30%to 17.83%,primarily due to the enhancement of short-circuit current density(JSC).Additionally,the introduction of BTP-eC7 also reduces the trap state density in the photoactive layer which helps to reduce the loss of *** study introduces a novel approach for employing ternary alloy models by incorporating dual acceptors with similar structures,and elucidates the underlying mechanism of charge generation and JSC in ternary OSCs.

关键词： Organic photovoltaics Ternary strategy Exciton diffusion Hole transfer Trap state Kinetics Alloys Photophysics

Biomimicking synovial joints trans-scale structured AgQDs/MXene/SiOC achieving macroscale high lubrication and superior wear resistance

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Journal of Materials Science & Technology 2024年第7期174卷 63-73页

作者： Yu Zhao Hui Mei peng Chang Yubo Yang Laifei cheng Litong Zhang Science and Technology on Thermostructural Composite Materials Laboratory School of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi’an710072China School of Textile Science and Engineering Xi’an Polytechnic UniversityXi’an710048China

Naturally optimized successful synovial joints with lightweight,high load-carrying,ultra-low friction and wear have attracted tribological communities to constantly imitate and *** impressive advances in cartilage lubrication,extending such extraordinary performance advantages to macroscale solid lubrication remains a ***,inspired by the fascinating interplay of synovial joints,a novel kind of trans-scale hierarchical structured ceramic-based composite was ***-ducing microscale Ag microspheres(AgMs)“cartilage”layer and nanoscale Ag quantum dots/MXene(AgQDs/MXene)“synovial fluid”into the interior and exterior of printed macroscale SiOC“hard bone”realistically restores the gradient structure of synovial joint *** resulted composite with ideal compressive strength(70.44 MPa)can achieve a 60.53%friction reduction and a low wear rate(2.05×10^(−6)mm 3 N^(−1)m^(−1))in dry tribo-contact for 3600 sliding cycles,while also maintaining considerable low friction(∼0.11)over 10,000 sliding cycles and long-term stable lubrication(∼0.13)for up to 50,000 re-ciprocating *** extraordinary performance can be explained by the division of macro contacts,full loading of AgMs and AgQDs/MXene,abrasive debris capture and removal,as well as the shear rolling effect induced by friction *** work opens a new avenue to develop structural lubricating mate-rials for complex engineering applications.

关键词： 3D printing Synovial joints trans-scale structure AgQDs/MXene/SiOC High lubrication Superior wear resistance

Amplifiable protein identification via residue-resolved barcoding and composition code counting

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National Science Review 2024年第7期11卷 205-217页

作者： Weiming Guo Yuan Liu Yu Han Huan Tang Xinyuan Fan chu Wang peng R.Chen Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular SciencesKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationCollege of Chemistry and Molecular Engineering Peking University Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University

Ultrasensitive protein identification is of paramount importance in basic research and clinical diagnostics but remains extremely challenging. A key bottleneck in preventing single-molecule protein sequencing is that, unlike the revolutionary nucleic acid sequencing methods that rely on the polymerase chain reaction(PCR) to amplify DNA and RNA molecules, protein molecules cannot be directly amplified. Decoding the proteins via amplification of certain fingerprints rather than the intact protein sequence thus represents an appealing alternative choice to address this formidable challenge. Herein, we report a proof-of-concept method that relies on residue-resolved DNA barcoding and composition code counting for amplifiable protein fingerprinting(AmproCode). In AmproCode, selective types of residues on peptides or proteins are chemically labeled with a DNA barcode, which can be amplified and quantified via quantitative PCR. The operation generates a relative ratio as the residue-resolved ‘composition code’ for each target protein that can be utilized as the fingerprint to determine its identity from the proteome database. We developed a database searching algorithm and applied it to assess the coverage of the whole proteome and secretome via computational simulations, proving the theoretical feasibility of AmproCode. We then designed the residue-specific DNA barcoding and amplification workflow, and identified different synthetic model peptides found in the secretome at as low as the fmol/L level for demonstration. These results build the foundation for an unprecedented amplifiable protein fingerprinting method. We believe that, in the future,AmproCode could ultimately realize single-molecule amplifiable identification of trace complex samples without further purification, and it may open a new avenue in the development of next-generation protein sequencing techniques.

关键词： protein identification amplifiable fingerprinting residue-specific chemistry DNA barcoding composition code counting

Interface strengthening for thermal sprayed WC-10Co4Cr coating subjected to pulsed magnetic treatment

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Rare Metals 2024年第2期43卷 780-795页

作者： cheng-Kai Qian Qu Liu Heng Wang Ke-Jian Li Zhi-peng Cai Department of Mechanical Engineering Tsinghua UniversityBeijing100084China Tianjin Research Institute for Advanced Equipment Tsinghua UniversityTianjin300304China State Key Laboratory of Tribology in Advanced Equipment Tsinghua UniversityBeijing100084China

The reliability of the coated industry components demands ideal fatigue properties of the coating,and it is mainly determined by the performance of the *** this study,pulsed magnetic treatment(PMT)was applied to the thermal sprayed WC-10Co4Cr coating,and the fatigue lifetime of the coated bolt increased by 219.82%under an imitation of the operating mode *** tests further proved that both the adhesion and cohesion strength were improved after PMT,and they benefit from the interface strengthening *** formation of coherent WC/Co interfaces was characterized by in-situ transmission electron microscopy(TEM),and the molecular dynamic simulations indicate that the work of separation of these interfaces is much higher than the original disordered *** stress was relaxed and distributed more homogeneously after PMT,and it mainly contributes to the coating/substrate *** work provides a new post-treatment method focusing on the interfaces in the WC-based coating and gives insight into its mechanism so that it is hopeful to be applied to other kinds of coatings.

关键词： Cemented carbide coating Pulsed magnetic treatment Interface strengthening Fatigue properties Adhesion strength

Effect of ceramic work rolls on surface roughness of rolled SUS304 ultra-thin strips

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Journal of Iron and Steel Research International 2024年第7期31卷 1704-1718页

作者： peng-cheng Li Tao Wang Chen-chen Zhao Qi Liu Qing-xue Huang College of Mechanical and Vehicle Engineering Taiyuan University of TechnologyTaiyuan 030024ShanxiChina Engineering Research Center of Advanced Metal Composites Forming Technology and Equipment of Ministry of Education Taiyuan University of TechnologyTaiyuan 030024ShanxiChina National Key Laboratory of Metal Forming Technology and Heavy Equipment Taiyuan University of TechnologyTaiyuan 030024ShanxiChina

Surface morphology and roughness are important parameters of surface quality of cold-rolled ultra-thin strip *** order to study the effect of Si_(3)N_(4) ceramic work rolls on the surface roughness of SUS304 ultra-thin strip,ABAQUS finite element model calculation,3D laser profilometry,and other methods were adopted based on the physical characteristics of Si_(3)N_(4) ceramic roll and 9Cr2MoV steel roll,like anti-flattening performance and oil *** surface morphology and roughness of SUS304 ultra-thin strip rolled by different material work rolls under oil lubrication conditions were *** results showed that under the rolling force of 10 kN,the maximum flattening amount of Si_(3)N_(4) ceramic roll was reduced by 35.14%compared to that of 9Cr2MoV steel *** reduction rate of SUS304 ultra-thin strip rolled by ceramic roll was 34.19%,while that rolled by steel roll was only 22%;Si_(3)N_(4) ceramic roll can further increase the number of alternating convex peaks and concave valleys in the rolled thin strip,reduce the profile steepness,reduce surface roughness,and enhance the planarization effect of surface micro-convex bodies,with improving surface quality and surface ***,the mechanism of Si_(3)N_(4) ceramic roll significantly improving the surface roughness of rolled SUS304 ultra-thin strip was proposed.

关键词： Ceramic work roll-Steel roll SUS304 ultra-thin strip Reduction Surface roughness

Numerical simulation study on the mold strength of magnetic mold casting based on a coupled electromagnetic-structural method

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China Foundry 2024年第5期21卷 577-587页

作者： Wei-li peng Jian-hua Zhao cheng Gu Ya-jun Wang College of Materials Science and Engineering Chongqing UniversityChongqing 400045China National Engineering Research Center for Magnesium Alloys Chongqing UniversityChongqing 400044China National Key Laboratory of Advanced Casting Technologies Chongqing UniversityChongqing 400044China

The properties of the magnetic mold in magnetic mold casting directly determine the quality of the final cast *** this study,the magnetic mold properties in magnetic mold casting,were studied utilizing a coupled electromagnetic-structural method through numerical *** study investigated key factors including equivalent stress,the distribution of tensile and compressive stresses,and the area ratio of tensile *** compared molds made entirely of magnetic materials with those made partially of magnetic *** results indicate that as current increases from 4 A to 8 A,both the initial magnetic mold and the material-replaced magnetic mold initially show an increasing trend in equivalent stress,tensile-compressive stress,and the area ratio of tensile stress,peaking at 6 A before *** material replacement,the area ratio of tensile stress at 6 A decreases to 19.84%,representing a reduction of 29.72%.Magnetic molds comprising a combination of magnetic and non-magnetic materials exhibit sufficient strength and a reduced area ratio of tensile stress compared to those made entirely from magnetic *** study provides valuable insights for optimizing magnetic mold casting processes and offers practical guidance for advancing the application of magnetic molds.

关键词： magnetic mold casting coupled electromagnetic-structural method numerical simulation stress analysis

Coexistence of All-Order Topological States in a Three-Dimensional Phononic Topological Crystalline Insulator

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Research 2024年第2期2023卷 511-518页

作者： Hua-Shan Lai Hao Chen chu-Hao Xia Si-Yuan Yu cheng He Yan-Feng Chen National Laboratory of Solid State Microstructures&Department of Materials Science and Engineering Nanjing UniversityNanjng 210093China Collaborative Innovation Center of Advanced Microstructures Nanjing University Nanjng 210093China Jiangsu Key Laboratory of Artificial Functional Materials Nanjing University Nanjing 210093China

Classical-wave topological materials lacking intrinsic half-integer spins are less robust while more ***,we explore a single 3-dimensional phononic topological crystalline insulator that simultaneously exhibits a whole family of first-order quadratic surface,second-order hinge,and third-order corner states within the same *** a topological crystalline insulator hosting all-order phases originates from the different topological nature when hierarchically projected onto different facets and lower dimensions,thus free from trivial cladding *** work offers an ideal platform for either robust wave propagation or localization in on-demand dimensions and may facilitate dimension division multiplexing technology.

关键词： topological dimensions crystalline