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Emulsion‑based evolution of Escherichia coli for higher growth yield on D‑xylose identifies central role of cyclic AMP

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Systems Microbiology and Biomanufacturing 2023年第4期3卷 730-738页

作者： James S.Orr Edwin Zen Xiaoyi Wang christopher v.rao DOE Center for Advanced Bioenergy and Bioproducts Innovation UrbanaILUSA Department of Chemical and Biomolecular Engineering University of Illinois at Urbana-Champaign600 S.Mathews Ave.UrbanaIL 61801USA Core Facility Center The First Affiliated Hospital of Nanjing Medical UniversityNanjingChina

D-xylose is an abundant sugar found in plant biomass and can be used as a renewable feedstock for the microbial production of diverse biofuels and ***,D-xylose metabolism is slow in many industrial microorganisms,at least as compared to glucose *** surprisingly,a number of approaches have been developed for improving D-xylose metabolism in diverse *** this work,we applied a previously developed evolution strategy based on media-in-oil emulsions for improving the growth yield of Escherichia coli NCM3722 on *** 30 rounds of evolutions,we isolated multiple mutants with increased growth yield on *** addition,we also observed similar increases in the growth *** mutants were selected for whole-genome *** mutants had an amber stop mutation in adenylate cyclase,which truncates nearly 60%of the ***,the ability of this mutant to grow on xylose indicated that truncated enzyme,lacking the C-terminal regulatory domain,is still *** other mutant had a point mutation in the cyclic AMP receptor protein(CRP),near the high affinity binding site for cyclic *** mutations,when intro-duced into wild type ***,were able to increase the growth yields at levels similar to the isolated *** addition to D-xylose,these mutant strains and their genetic mimics also exhibited higher growth rates and yields on glucose,lactose,and *** results suggest that the improved growth rates and yields are due to changes in the production and sensing of intracellular cyclic AMP concentrations and also suggest native concentrations are suboptimal with respect to the growth rate and yield under the growth conditions ***,these results may prove useful for engineering strains of *** for high-density fermentations or protein production.

关键词： Escherichia coli Xylose cAMP Adaptive laboratory evolution

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How would preclinical Alzheimer’s disease (AD pathology) occur? An insight from a genomic instability mouse model

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Neural Regeneration Research 2021年第10期16卷 2012-2014页

作者： Chinthalapally v.rao Hiroshi Y.Yamada Center for Cancer Prevention and Drug Development Department of MedicineHematology/Oncology SectionUniversity of Oklahoma Health Sciences Center(OUHSC)Oklahoma CityOKUSA

More than 95%of Alzheimer’s disease(AD)is late-onset,in which patients show clinical cognition/behavior symptoms after age *** early-onset AD that comes with mutations in genes directly involved in amyloid metabolism(APP,PSEN),genetic predispositions associated with late-onset AD are harder to pinpoint,and their mechanistic links to AD development need further *** the development mechanism of late-onset AD remains controversial,amyloid-beta accumulation,initiated in middle age,is widely accepted as the triggering event for early AD pathology(Du Bois et al.,2010;Sterling et al.,2011).

关键词： clinical Alzheimer metabolism

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Phytochemical investigation, antioxidant and wound healing activities of Citrullus colocynthis (bitter apple)

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Asian Pacific Journal of Tropical Biomedicine 2018年第8期8卷 418-424页

作者： Sateesh Chandra Gupta Tusha Tripathi Shravan Kumar Paswan Annie G.Agarwal Chandana v.rao Om P.Sidhu Department of Phytochemistry CSIR-National Botanical Research Institute Department of Pharmacology CSIR-National Botanical Research Institute

Objective: To undertake metabolite profiling of various plant parts of Citrullus colocynthis, and assess antioxidant and wound healing activities of fractions for therapeutical applications. Methods: Extracts from leaves, stem, root, fruit pulp and seeds were analyzed using gas chromatography-mass spectrometry and high performance liquid chromatography. variation in antioxidant potential was assayed by 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical scavenging assay. The extract with highest antioxidant potential was subjected on in-vivo wound healing activity using excision wound model. Results: Metabolite profiling of Citrullus colocynthis identified 70 chemically diverse metabolites from different plant parts by using a combination of GC-MS and HPLC. Concentration of colocynthin, a principal active secondary metabolite, ranged from 3.15 mg/g dry weight to 242.00 mg/g dry weight, the lowest being in leaves and highest in fruit pulp. DPPH radical scavenging activity of free radical(IC50) ranged from 196.44 μg/mL in fruit pulp to 413.33 μg/mL in leaves tissues. Significant wound contraction and increase in hydroxyproline content of granulation tissue were observed with ointment formulated from methanolic extract of fruit pulp. Conclusions: The study indicates that the methanol extract of Citrullus colocynthis fruit pulp when applied topically may promote wound contraction in rat model attributable to the accumulation of colocynthin. The high quantity of colocynthin(242.00 mg/g dry weight) and substantial concentration of 2,4-di-tert butyl phenol(3.2%), squalene(4.2%) and δ-tocopherol(2.5%) make this plant to provide new opportunities for development of medicinal, nutraceutical and dietary supplements with optimized functionality.

关键词： Metabolite profiling Citrullus colocynthis GC-MS Antioxidant Wound healing

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The Current State of Transradial Access:A Perspective on Transradial Outcomes,Learning Curves,and Same-Day Discharge

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Cardiovascular Innovations and Applications 2018年第B7期3卷 149-162页

作者： Jennifer A.Rymer Sunil v.rao Duke Clinical Research Institute DurhamNCUSA

The adoption of transradial access in the United States and internationally has been growing over the past few *** the population of patients presenting with acute coronary syndromes,particularly ST-elevation myocardial infarction,transradial access has the benefit of fewer vascular and bleeding complications and lower mortality rates over transfemoral *** will examine the current evidence supporting transradial access for several patient populations,including those patients presenting with acute coronary *** will review the literature regarding the learning curve for transradial access with new operators,as well as experienced transfemoral operators new to transradial ***,we will investigate the role of transradial access in same-day discharge for stable patients undergoing percutaneous coronary intervention.

关键词： transradial percutaneous coronary intervention learning curves outcomes same-day discharge

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三白草的化学成分研究(Ⅰ)

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中草药 2001年第1期32卷 9-11页

作者：马敏阮金兰 Koppaka v.rao 同济医科大学药学院湖北武汉430030 美国佛罗里达大学药学院

目的　研究三白草 Saururus chinensis(L our.) Baill.的化学成分。方法　采用硅藻土柱层析和反复硅胶柱层析分离纯化 ,通过理化常数测定和光谱分析鉴定其化学结构。结果　从三白草中分得了 3个化合物 ,即三白脂素(saucernetin, )、三... 详细信息

目的　研究三白草 Saururus chinensis(L our.) Baill.的化学成分。方法　采用硅藻土柱层析和反复硅胶柱层析分离纯化 ,通过理化常数测定和光谱分析鉴定其化学结构。结果　从三白草中分得了 3个化合物 ,即三白脂素(saucernetin, )、三白脂素 - 8(saucernetin- 8, )和三白脂素 - 7(saucernetin- 7, )。

关键词：三白草三白脂素三白脂素-8 三白脂素-7 硅藻土柱层析硅胶柱层析

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Superconducting magnetic energy storage for stabilizing grid integrated with wind power generation systems

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Journal of Modern Power Systems and Clean Energy 2019年第2期7卷 400-411页

作者： Poulomi MUKHERJEE v. v. rao Applied Superconductivity Laboratory Cryogenic Engineering Centre Indian Institute of TechnologyKharagpur

Due to the interconnection of various renewable energies and adaptive technologies, voltage quality and frequency stability of modern power systems are becoming erratic. Superconducting magnetic energy storage(SMES),for its dynamic characteristic, is very efficient for rapid exchange of electrical power with grid during small and large disturbances to address those *** addition,SMES plays an important role in integrating renewable sources such as wind generators to power grid by controlling output power of wind plant and improving the stability of power *** application of SMES in various power system operations depends on the proper location in the power system, exact energy and power ratings and appropriate controllers. In this paper, an effort is given to explain SMES device and its controllability to mitigate the stability of power grid integrated with wind power generation systems.

关键词： Power fluctuation Power quality Low voltage ride through Superconducting magnetic energy storage Superconductors Wind energy

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Crimean-Congo hemorrhagic fever:Pathogenesis,transmission and public health challenges

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World Journal of virology 2025年第1期14卷 63-72页

作者： Sita Kumari Karanam Kandra Nagvishnu Praveen Kumar Uppala Sandhya Edhi Srinivasa rao varri Department of Pharmaceutical Biotechnology Maharajah’s College of PharmacyVizianagaram 535002Andhra PradeshIndia Department of Pharmacology Santhiram Medical College and General HospitalNandyal 518501Andhra PradeshIndia Department of Pharmacology Santhiram Medical College and General HospitalNandyal 518501Andhra PradeshIndia Department of Pharmaceutical AnalysisMaharajah's College of PharmacyVizianagaram 535002Andhra PradeshIndia

The dangerous Crimean-Congo hemorrhagic fever virus(CCHFv),an encapsulated negative-sense RNA virus of the family Nairoviridae,is transmitted from person to person via *** a case fatality rate between 10%to 40%,the most common ways that the disease may spread to humans are via tick bites or coming into touch with infected animals'blood or ***,the transfer of bodily fluids between individuals is another potential route of *** is a wide range of symptoms experienced by patients throughout each stage,from myalgia and fever to extreme bruising and excess *** management measures include minimising the spread of ticks from one species to another and from people to animals via the use of protective clothing,repellents,and proper animal *** order to prevent the spread of illness,healthcare workers must adhere to stringent *** the lack of an authorised vaccine,the main components of treatment now consist of preventative measures and supportive care,which may include the antiviral medicine *** still don't know very much about the virus's mechanisms,even though advances in molecular virology and animal models have improved our understanding of the pathogenesis of CCHFv.A critical need for vaccination that is both safe and effective,as well as for quick diagnosis and efficient treatments to lessen the disease's impact in areas where it is most *** steps towards lowering Crimean-Congo hemorrhagic fever mortality and morbidity rates were to anticipatethe future availability of immunoglobulin products.

关键词： Crimean-congo haemorrhagic fever Tick-borne illness Immunoglobulins viral hemorrhagic fever Antiviral therapy

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Parametric optimization of friction stir welding process of age hardenable aluminum alloys-ANFIS modeling

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Journal of Central South University 2016年第8期23卷 1847-1857页

作者： D.vijayan v.Seshagiri rao Department of Mechanical Engineering Sri Chandrasekharendra Saraswathi Viswa Maha Vidyalaya Enathur Kanchipuram- 631561 Tamilnadu India St. Joseph's College of Engineering Chennai - 600025 Tamilnadu India

A comparative approach was performed between the response surface method(RSM) and the adaptive neuro-fuzzy inference system(ANFIS) to enhance the tensile properties, including the ultimate tensile strength and the tensile elongation, of friction stir welded age hardenable AA6061 and AA2024 aluminum alloys. The effects of the welding parameters, namely the tool rotational speed, welding speed, axial load and pin profile, on the ultimate tensile strength and the tensile elongation were analyzed using a three-level, four-factor Box-Behnken experimental design. The developed design was utilized to train the ANFIS models. The predictive capabilities of RSM and ANFIS were compared based on the root mean square error, the mean absolute error, and the correlation coefficient based on the obtained data set. The results demonstrate that the developed ANFIS models are more effective than the RSM model.

关键词： aluminum alloys response surface method(RSM) adaptive neuro-fuzzy inference system(ANFIS) friction stir welding Box-Behnken design neuro fuzzy

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Performance analysis of ASR system in hybrid DNN-HMM framework using a PWL euclidean activation function

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Frontiers of Computer Science 2021年第4期15卷 185-195页

作者： Anirban DUTTA Gudmalwar ASHISHKUMAR Ch v Rama rao Department of Electronics and Communication Engineering National Institute of Technology MeghalayaShillong 793003India

Automatic Speech Recognition(ASR)is the process of mapping an acoustic speech signal into a human readable text *** systems exploit the Acoustic Component of ASR using the Gaussian Mixture Model-Hidden Markov Model(GMM-HMM)*** NeuralNetwork(DNN)opens up new possibilities to overcome the shortcomings of conventional statistical *** studies modeled the acoustic component of ASR system using DNN in the so called hybrid DNN-HMM *** the context of activation functions used to model the non-linearity in DNN,Rectified Linear Units(ReLU)and maxout units are mostly used in ASR *** paper concentrates on the acoustic component of a hybrid DNN-HMM system by proposing an efficient activation function for the DNN *** by previous works,euclidean norm activation function is proposed to model the non-linearity of the DNN *** non-linearity is shown to belong to the family of Piecewise Linear(PWL)functions having distinct *** functions can capture deep hierarchical features of the *** relevance of the proposal is examined in depth both theoretically and *** performance of the developed ASR system is evaluated in terms of Phone Error Rate(PER)using TIMIT *** results achieve a relative increase in performance by using the proposed function over conventional activation functions.

关键词： deep learning euclidean piecewise linear speech recognition activation function

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A novel approach for speaker diarization system using TMFCC parameterization and Lion optimization

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Journal of Central South University 2017年第11期24卷 2649-2663页

作者： v.Subba Ramaiah R.Rajeswara rao Mahatma Gandhi Institute of Technology KokapetHyderabadTelangana 500075India Jawaharlal Nehru Technological University Kakinada KakinadaAndhra Pradesh 535002India

In audio stream containing multiple speakers, speaker diarization aids in ascertaining "who speak when". This is an unsupervised task as there is no prior information about the speakers. It labels the speech signal conforming to the identity of the speaker, namely, input audio stream is partitioned into homogeneous segments. In this work, we present a novel speaker diarization system using the Tangent weighted Mel frequency cepstral coefficient(TMFCC) as the feature parameter and Lion algorithm for the clustering of the voice activity detected audio streams into particular speaker groups. Thus the two main tasks of the speaker indexing, i.e., speaker segmentation and speaker clustering, are improved. The TMFCC makes use of the low energy frame as well as the high energy frame with more effect, improving the performance of the proposed system. The experiments using the audio signal from the ELSDSR corpus datasets having three speakers, four speakers and five speakers are analyzed for the proposed system. The evaluation of the proposed speaker diarization system based on the tracking distance, tracking time as the evaluation metrics is done and the experimental results show that the speaker diarization system with the TMFCC parameterization and Lion based clustering is found to be superior over existing diarization systems with 95% tracking accuracy.

关键词： speaker diarization Mel frequency cepstral coefficient i-vector extraction Lion algorithm

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