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Development of a standardized in vitro approach to evaluate microphysical,chemical,and toxicological properties of combustion-derived fine and ultrafine particles

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Journal of Environmental Sciences 2022年第3期34卷 104-117页

作者： Ana Teresa Juarez-Facio Clement Castilla Cecile Corbiere Helene Lavanant Carlos Afonso christophe morin Nadine Merlet-Machour Laurence Chevalier Jean-Marie Vaugeois Jerome Yon Christelle Monteil Normandie Univ UNIROUENUNICAEN ABTE76000 RouenFrance Normandie Univ UNIROUENINSA RouenCNRSCOBRA76000 RouenFrance Normandie Univ UNIROUENINSA RouenCNRSGPM-UMR663476000 RouenFrance Normandie Univ UNIROUENINSA RouenCNRSCORIA76000 RouenFrance

Ultrafine particles represent a growing concern in the public health community but their precise role in many illnesses is still unknown. This lack of knowledge is related to the experimental difficulty in linking their biological effects to their multiple properties, which are important determinants of toxicity. Our aim is to propose an interdisciplinary approach to study fine(FP) and ultrafine(UFP) particles, generated in a controlled manner using a mini CAST(Combustion Aerosol Standard) soot generator used with two different operating conditions(CAST1 and CAST3). The chemical characterization was performed by an untargeted analysis using ultra-high resolution mass spectrometry. In conjunction with this approach, subsequent analysis by gas chromatography–mass spectrometry(GC–MS) was performed to identify polycyclic aromatic hydrocarbons(PAH). CAST1 enabled the generation of FP with a predominance of small PAH molecules, and CAST3 enabled the generation of UFP, which presented higher numbers of carbon atoms corresponding to larger PAH molecules. Healthy normal human bronchial epithelial(NHBE) cells differentiated at the air-liquid interface(ALI) were directly exposed to these freshly emitted FP and UFP. Expression of MUC5AC, FOXJ1, OCLN and ZOI as well as microscopic observation confirmed the ciliated pseudostratified epithelial phenotype. Study of the mass deposition efficiency revealed a difference between the two operating conditions, probably due to the morphological differences between the two categories of particles. We demonstrated that only NHBE cells exposed to CAST3 particles induced upregulation in the gene expression of IL-8 and NQO1. This approach offers new perspectives to study FP and UFP with stable and controlled properties.

关键词： In vitro Air-liquid interface MiniCAST Fine and ultrafine particles Organic compounds OC/TC

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黄芪多糖对过氧化氢刺激皮肤成纤维细胞中线粒体和溶酶体的保护作用(英文)

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中国病理生理杂志 2008年第4期24卷 777-782页

作者：岳晓莉李萍刘欣黄启福 BARBIER-CHASSEFIERE Veronique morin christophe PAPY-GARCIA Dulce 北京中医药大学病理教研室北京100029 首都医科大学附属北京中医医院北京市中医研究所生理室北京100010 法国国家科研中心 UMR 7149 巴黎第十二大学组织再生与修复实验室94010 克雷地亚法国

目的:证明黄芪多糖对氧化应激皮肤细胞的保护作用。方法:原代培养皮肤成纤维细胞在过氧化氢0.5 mmol/L中,孵育30 min造成急性氧化损伤的模型。MTT法测黄芪多糖的剂量反应,DAPI染色法检测黄芪多糖对细胞死亡的作用;激光共聚焦法检测黄芪... 详细信息

目的:证明黄芪多糖对氧化应激皮肤细胞的保护作用。方法:原代培养皮肤成纤维细胞在过氧化氢0.5 mmol/L中,孵育30 min造成急性氧化损伤的模型。MTT法测黄芪多糖的剂量反应,DAPI染色法检测黄芪多糖对细胞死亡的作用;激光共聚焦法检测黄芪多糖对线粒体、线粒体膜电位以及溶酶体稳定性的保护作用。结果:经过黄芪多糖的治疗,能够明显提高细胞存活率,其作用浓度从0.5 mg/L开始,到1 mg/L达最大效果,呈剂量依赖性。而且,黄芪多糖能够抑制线粒体膜电位崩解保护线粒体形态,保护溶酶体膜。结论:这些结果证明了黄芪多糖在线粒体和溶酶体水平存在1个新的调节凋亡的途径。这个途径可以成为一些氧化应激以及溶酶体缺陷疾病的1个新的治疗靶点。

关键词：氧化性应激线粒体溶酶体黄芪多糖

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The miR166-SIHB15A regulatory module controls ovule development and parthenocarpic fruit set under adverse temperatures in tomato

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Molecular Plant 2021年第7期14卷 1185-1198页

作者： Christian Clepet Ravi Sureshbhai Devani Rachid Boumlik Yanwei Hao Halima morin Fabien Marcel Marion Verdenaud Brahim Mania Gwilherm Brisou Sylvie Citerne Gregory Mouille Jean-christophe Lepeltier Shai Koussevitzky Adnane Boualem Abdelhafid Bendahmane Institute of Plant Sciences Paris-Saclay INRAECNRSUniversite Paris-SaclayOrsay 91405France HM Clause Mas Saint-PierreQuartier La GalineSaint-Remy de Provence 13210France Hazera Seeds Ltd Berurim M.P.Shikmim 7983700Israel lnstitut Jean-Pierre Bourgin INRAEVersaillesFrance Present address:Key Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China College of HorticultureSouth China Agricultural UniversityGuangzhou 510642China

Fruit set is inhibited by adverse temperatures,with consequences on yield.We isolated a tomato mutant producing fruits under non-permissive hot temperatures and identified the causal gene as SIHB15A,belonging to classⅢhomeodomain leucine-zipper transcription factors.SIHB15A,loss-of-function mu-tants display aberrant ovule development that mimics transcriptional changes occurring in fertilized ovules and leads to parthenocarpic fruit set under optimal and non-permissive temperatures,in field and greenhouse conditions.Under cold growing conditions,SIHB15A is subjected to conditional haploinsufficiency and recessive dosage sensitivity controlled by microRNA 166(miR166).Knockdown of SIHB15A alleles by miR166 leads to a continuum of aberrant ovules correlating with parthenocarpic fruit set.Consistent with this,plants harboring an Slhb15a-miRNA166-resisiant allele developed normal ovules and were unable to set parthenocarpic fruit under cold conditions.DNA affinity purification sequencing and RNA-sequencing analyses revealed that SIHB15A is a bifunctional transcription factor expressed in the ovule integument.SIHB15A binds to the promoters of auxin-related genes to repress auxin signaling and to the promoters of ethylene-related genes to activate their expression.A survey of tomato genetic biodiversity identified pat and pat-1,two historical parthenocarpic mutants,as alleles of SIHB15A.Taken together,our findings demonstrate the role of SIHB15A as a sentinel to prevent fruit set in the absence of fertilization and provide a mean to enhance fruiting under extreme temperatures.

关键词： parthenocarpy dosage sensitivity HD-Ziplll miRNA166 heat stress cold stress

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