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A quantum cascade laser infrared spectrometer for CO_2 stable isotope analysis：Field implementation at a hydrocarbon contaminated site under bio-remediation

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Journal of Environmental Sciences 2016年第2期28卷 60-74页

作者： christophe Guimbaud Cécile Noel Michel Chartier Valéry Catoire Michaela Blessing Jean christophe Gourry Claude Robert Laboratoire de Physique et de Chimie de l'Environnement et de l'Espace (LPC2E) CNRS et Universite d'Orleans (UMR 7328)45071 Orleans cedex 2 France Bureau de Recherches Geologiques et Minieres (BRGM) 45060 Orleans cedex 2 France

Real-time methods to monitor stable isotope ratios of CO_2 are needed to identify biogeochemical origins of CO_2 emissions from the soil–air interface. An isotope ratio infra-red spectrometer（IRIS） has been developed to measure CO_2 mixing ratio with δ~13C isotopic signature, in addition to mixing ratios of other greenhouse gases（CH_4, N2_O）. The original aspects of the instrument as well as its precision and accuracy for the determination of the isotopic signature δ~13C of CO_2 are discussed. A first application to biodegradation of hydrocarbons is presented, tested on a hydrocarbon contaminated site under aerobic bio-treatment. CO_2 flux measurements using closed chamber method is combined with the determination of the isotopic signature δ~13C of the CO_2 emission to propose a non-intrusive method to monitor in situ biodegradation of hydrocarbons. In the contaminated area, high CO_2 emissions have been measured with an isotopic signature δ~13C suggesting that CO_2 comes from petroleum hydrocarbon *** first field implementation shows that rapid and accurate measurement of isotopic signature of CO_2 emissions is particularly useful in assessing the contribution of contaminant degradation to the measured CO_2 efflux and is promising as a monitoring tool for aerobic bio-treatment.

关键词： Isotope ratio infrared spectrometry Isotopic signature δ^(13)C of CO_2 Biodegradation monitoring Hydrocarbons BTEX

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Regulation of CD137 expression through K-Ras signaling in pancreatic cancer cells

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Cancer Communications 2019年第1期39卷 379-389页

作者： christophe Glorieux Peng Huang Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhou 510060GuangdongP.R.China

Background:The interaction between CD137 and its ligand(CD137L)plays a major role in the regulation of immune functions and affects cancer ***137 is a cell surface protein mainly located on activated T cells,and its regulation and functions in immune cells are well ***,the expression of CD137 and its regulation in cancer cells remain poorly *** main purposes of this study were to examine the expression of CD137 in pancreatic cancer cells and to investigate its underlying ***:Cells containing inducible K-RasG12V expression vector or with different K-Ras mutational statuses were used as in vitro models to examine the regulation of CD137 expression by *** molecular assays were employed to explore the regulatory *** specimens from 15 pancreatic cancer patients and serum samples from 10 patients and 10 healthy donors were used to test if the expression of CD137 could be validated in clinical ***:We found that the CD137 protein was expressed on the cell surface in pancreatic cancer tissues and cancer cell ***-linked immunosorbent assay revealed no difference in the levels of secreted CD137 in the sera of patients and healthy *** using the K-Ras inducible cell system,we further showed that oncogenic K-Ras up-regulated CD137 through the activation of MAPK(mitogen-activated protein kinases)and NF-κB(nuclear factor kappa-light-chain-enhancer of activated B cells)pathways,as evidenced by significantly reduced CD137 mRNA expression led by genetic silencing of MAPK1 and p65,the key proteins involved in the respective ***-more,we also found that the NF-κB pathway was mainly stimulated by the K-Ras-induced secretion of interleukin-1α(IL-1α)which promoted the transcription of the CD137 gene in pancreatic cancer cell *** of the TCGA(the cancer genome atlas)database also revealed a significant correlation between IL-1αand CD137 expression(r=0.274)in tumor s

关键词： CD137 K-Ras IL-1α(interleukin-1 alpha) MAPK(mitogen-activated protein kinases) NF-κB(nuclear factor kappa-light-chain-enhancer of activated B cells) Pancreatic cancer Oxidative stress Soluble CD137

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Experiments on thermo-hydro-mechanical behaviour of Opalinus Clay at Mont Terri rock laboratory, Switzerland

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Journal of Rock Mechanics and Geotechnical Engineering 2017年第3期9卷 120-128页

作者： Paul Bossart David Jaeggi christophe Nussbaum Swiss Geological Survey at swisstopo Wabern Switzerland

Repositories for deep geological disposal of radioactive waste rely on multi-barrier systems to isolate waste from the biosphere.A multi-barrier system typically comprises the natural geological barrier provided by the repository host rock e in our case the Opalinus Clay e and an engineered barrier system(EBS).The Swiss repository concept for spent fuel and vitrified high-level waste(HLW)consists of waste canisters,which are emplaced horizontally in the middle of an emplacement gallery and are separated from the gallery wall by granular backfill material(GBM).We describe here a selection of five in-situ experiments where characteristic hydro-mechanical(HM)and thermo-hydro-mechanical(THM)processes have been *** first example is a coupled HM and mine-by test where the evolution of the excavation damaged zone(EDZ)was monitored around a gallery in the Opalinus Clay(ED-B experiment).Measurements of pore-water pressures and convergences due to stress redistribution during excavation highlighted the HM *** same measurements were subsequently carried out in a heater test(HE-D)where we were able to characterise the Opalinus Clay in terms of its THM *** yielded detailed data to better understand the THM behaviours of the granular backfill and the natural host *** a presentation of the Swiss concept for HLW storage,we designed three demonstration experiments that were subsequently implemented in the Mont Terri rock laboratory:(1)the engineered barrier(EB)experiment,(2)the in-situ heater test on key-THM processes and parameters(HE-E)experiment,and(3)the full-scale emplacement(FE)*** first demonstration experiment has been dismantled,but the last two ones are on-going.

关键词： Deep geological disposal Radioactive waste Demonstration experiments Engineered barrier system(EBS) Granular backfill material(GBM) Bentonite Thermo-hydro-mechanical(THM) behaviour

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Numerical investigation of electric field distortion induced by internal defects in composite insulators

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High Voltage 2017年第4期2卷 253-260页

作者： Gbah Kone christophe Volat Hassan Ezzaidi Modeling and Diagnostic of Power Network Equipment Laboratory University of Quebec at Chicoutimi555 Bd.de l'UniversitéQCCanada G7H 2B1

This paper presents an exhaustive study of numerical investigations based on three-dimensional finite-element method modelling of several non-ceramic insulators of 69,110 and 230 kV having different internal semi-conductive defects in terms of size and *** internal defects were positioned close to the high-voltage electrode,the ground electrode and at floating *** simulations effected on the distortion of the axial and radial E-field components close to the insulator shank between *** results obtained showed that the radial E-field component is more sensitive to the presence of an internal defect as it shows a greater distortion in presence of the *** was also observed that the E-field component distortion increases with longer internal defect and that this increase is particularly important for a defect at floating ***,it was demonstrated that the length of the insulator,for a defect of constant length,has no significant influence on the E-field component ***,the results demonstrated that the presence of a grading ring,as used on the 230 kV insulator,does not have any influence on the E-field component distortion,independently of the defect position.

关键词： distortion insulator defect

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A reassessment of the diversity of green toads(Bufotes)in the circum-Aegean region

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Integrative Zoology 2021年第3期16卷 420-428页

作者： christophe DUFRESNES Nikolas MPROBONAS Ilias STRACHINIS LASER College of Biology and the EnvironmentNanjing Forestry UniversityNanjingPeople’s Republic of China Filoti NaxosGreece Department of Genetics Development and Molecular BiologySchool of BiologyFaculty of Natural SciencesAristotle University of ThessalonikiThessalonikiGreece

The dynamic biogeography of glacial refugia may cause complex patterns of genetic admixture between parapatric taxa,which in turn can mislead their systematics,diversity,and *** investigated this issue for green toads(Bufotes)inhabiting the circum-Aegean region,a biodiversity hotspot of the Eastern Mediterranean.A previous phylogeographic study based on mitochondrial and microsatellite loci identified the hybrid zone between the European(viridis)and Anatolian(sitibundus)lineages of *** all over the Balkan Peninsula,but subsequent range-wide genomic analyses(>1000 SNPs)located this transition in Turkey,a thousand kilometers *** order to clarify the diversity and taxonomy of the circum-Aegean populations,we reconciled these conflicting findings by integrating previous data with pure sitibundus *** results confirmed that the viridis/sitibundus hybrid zone extends in Western Anatolia,but that southeastern European populations feature cytonuclear discordances and a high and structured microsatellite *** remarkable situation may stem from a massive geographic displacement of the hybrid zone during the last glacial fluctuations,an underappreciated yet seemingly common feature among the herpetofauna of the *** study thus contributes to the rising view that mitochondrial DNA can be a poor predictor of current phylogeographic structure,hence the need for genomic data,especially for narrowly distributed ***,the analyses unambiguously support the distinction of a micro-endemic clade of green toads unique to some Cyclades islands,for which we provide a formal taxonomic description.

关键词： Cyclades hybrid zone Naxos phylogeography speciation

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Trip-oriented travel time prediction （TOTTP） with historical vehicle trajectories

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Frontiers of Earth Science 2018年第2期12卷 253-263页

作者： Tao XU Xiang LI christophe CLARAMUNT Key Laboratory of Geographic Information Science (Ministry of Education) East China Normal University Shanghai 200241 China School of Geographic Sciences East China Normal University Shanghai 200241 China The French Naval Academy Research Institute Lanveoc-Poulmic BP 600 29240 Brest Naval France

Accurate travel time prediction is undoubtedlyof importance to both traffic managers and travelers. Inhighly-urbanized areas, trip-oriented travel time prediction（TOTTP） is valuable to travelers rather than trafficmanagers as the former usually expect to know the traveltime of a trip which may cross over multiple road *** are two obstacles to the development of TOTTP,including traffic complexity and traffic data coverage. Withlarge scale historical vehicle trajectory data and meteorol-ogy data, this research develops a BPNN-based approachthrough integrating multiple factors affecting trip traveltime into a BPNN model to predict trip-oriented travel timefor OD pairs in urban network. Results of experimentsdemonstrate that it helps discover the dominate trends oftravel time changes daily and weekly, and the impact ofweather conditions is non-trivial.

关键词： trip-oriented travel time prediction (TOTTP) urban network Back Propagation Neural Networks(BPNN) historical vehicle trajectories

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From Squalene to Brassinolide： The Steroid Metabolic and Signaling Pathways across the Plant Kingdom

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Molecular Plant 2013年第6期6卷 1738-1757页

作者： Cdcile Vriet Eugenia Russinova christophe Reuzeau CropDesign NV a BASF Plant Science Company 9052 Gent Belgium Present address: Laboratoire de G~n~tique et-de Biophysique c/es Plantes Aix-Marseille UniversitY Marseille F-13009 France Present address: Centre National de la Recherche Scientifique Unite Mixte de Recherche Biologie Vc~gEtale et de Microbiologie Environmentale MarseilleF-13009 France Present address: Commissariat TEnergie Atomique Direction des Sciences du Vivant Institut de Biologie Environnementale et Biotechnologie MarseilleF-13009 France Department of Plant Systems Biology VIB 9052 Gent Belgium Department of Plant Biotechnology and Bioinformatics Ghent University 9052 Gent Belgium

The plant steroid hormones, brassinosteroids （BRs）, and their precursors, phytosterols, play major roles in plant growth, development, and stress tolerance. Here, we review the impressive progress made during recent years in elucidating the components of the sterol and BR metabolic and signaling pathways, and in understanding their mecha- nism of action in both model plants and crops, such as Arabidopsis and rice. We also discuss emerging insights into the regulations of these pathways, their interactions with other hormonal pathways and multiple environmental signals, and the putative nature of sterols as signaling molecules.

关键词： Arabidopsis rice phytosterols brassinosteroids.

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Experimental Analysis of the Rotor Stator Interaction Within a High Pressure Centrifugal Compressor

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Journal of Thermal Science 2002年第1期11卷 1-9页

作者： Isabelle Trébinjac christophe Vixège Laboratoire de Mécanique des Fluides et d’Acoustique Ecole Centrale de Lyon Ecully Cedex France

An analysis of the experimental results obtained by laser two focus anemometry in the rotor-stator inter-row region of a high-speed high-pressure centrifugal compressor is presented with the aim of quantifying the unsteady effects of the rotor-stator interaction. Two complementary methods are used. First, the results are analyzed in terms of the time-averaging procedure developed by Adamczyk. The purely time dependent correlations of the velocity components are quantified. They show that the unsteady interaction is mainly due to the splitter wake-vane interaction. Then, it is shown that the rotor-stator interaction produces spatial harmonics which are identified as spinning modes by means of Fourier series.

关键词： rotor-stator interaction centrifugal compressor spectral analysis.

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Slow and steady wins the race: contrasted phylogeographic signatures in two Alpine amphibians

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Integrative Zoology 2022年第1期17卷 181-190页

作者： christophe DUFRESNES Nicolas RODRIGUES Romain SAVARY LASER College of Biology and EnvironmentNanjing Forestry UniversityNanjingChina Department of Ecology&Evolution University of LausanneLausanneSwitzerland Laboratory for Biological Geochemistry School of ArchitectureCivil and Environmental EngineeringEcole Polytechnique Fédérale de Lausanne(EPFL)LausanneSwitzerland

A deeper phylogeographic structure is expected for slow-dispersing habitat specialists compared to widespread adaptable species,especially in topographically complex *** tested this classic assumption by comparing the genomic(RAD-sequencing)phylogeographies of 2 amphibians inhabiting the Swiss Alps:the mobile,cosmopolitan common frog(Rana temporaria)against the stationary,mountain endemic Alpine salamander(Salamandra atra).Our results ran opposite of predictions:the frog displayed significantly higher genetic divergences and lower within-population variation compared to the *** implies a prominent role for their distinctive glacial histories in shaping intraspecific diversity and structure:diversification and recolonization from several circum-Alpine micro-refugia for the frog versus a single refugium for the salamander,potentially combined with better population connectivity and *** striking differences emphasize the great variability of phylogeographic responses to the Quaternary glaciations,hence the complexity to predict general patterns of genetic diversity at the regional scale,and the forces that underlie them.

关键词： Alps comparative phylogeography dispersal RAD-sequencing Rana temporaria Salamandra atra

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Effect of wind stress forcing on ocean dynamics at air-sea interface

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Frontiers of Information Technology & Electronic Engineering 2018年第8期19卷 1056-1062页

作者： Hussein YAHIA Veronique GARCON Joel SUDRE christophe MAES Research Center INRIA Bordeaux-South West Talence 33405France CNRS LEGOS LaboratoryToulouse 31400France Brest University CNRSIRDIFREMERLOPSIUEMBrest 29238France

We evidence and study the differences in turbulence statistics in ocean dynamics carried by wind forcing at the air-sea interface. Surface currents at the air-sea interaction are of crucial importance because they transport heat from low to high latitudes. At first order, oceanic currents are generated by the balance of the Coriolis and pressure gradient forces(geostrophic current) and the balance of the Coriolis and the frictional forces dominated by wind stress(Ekman current) in the surface ocean layers. The study was conducted by computing statistical moments on the shapes of spectra computed within the framework of microcanonical multi-fractal formalism. Remotely sensed daily datasets derived from one year of altimetry and wind data were used in this study, allowing for the computation of two kinds of vector fields: geostrophy with and geostrophy without wind stress forcing. We explore the statistical properties of singularity spectra computed from velocity norms and vorticity data, notably in relation with kurtosis information to underline the differences in the turbulent regimes associated with both kinds of velocity fields.

关键词： Ocean dynamics Remote sensing Turbulence Signal processing Multi-fractal formalism

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