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Effects of P301L-TAU on post-translational modifications of microtubules in human iPSC-derived cortical neurons and TAU transgenic mice

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Neural Regeneration Research 2025年第8期20卷 2348-2360页

作者： Mohamed Aghyad Al Kabbani christoph Köhler Hans Zempel Institute of Human Genetics Faculty of Medicine and University Hospital CologneUniversity of CologneCologneGermany Center for Molecular Medicine Cologne(CMMC) University of CologneCologneGermany Center Anatomy Department ⅡMedical FacultyUniversity of CologneCologneGermany

TAU is a microtubule-associated protein that promotes microtubule assembly and stability in the *** is missorted and aggregated in an array of diseases known as *** are essential for neuronal function and regulated via a complex set of post-translational modifications,changes of which affect microtubule stability and dynamics,microtubule interaction with other proteins and cellular structures,and mediate recruitment of microtubule-severing *** impairment of microtubule dynamics causes neuronal dysfunction,we hypothesize cognitive impairment in human disease to be impacted by impairment of microtubule *** therefore aimed to study the effects of a disease-causing mutation of TAU(P301L)on the levels and localization of microtubule post-translational modifications indicative of microtubule stability and dynamics,to assess whether P301L-TAU causes stability-changing modifications to *** investigate TAU localization,phosphorylation,and effects on tubulin post-translational modifications,we expressed wild-type or P301L-TAU in human MAPT-KO induced pluripotent stem cell-derived neurons(i Neurons)and studied TAU in neurons in the hippocampus of mice transgenic for human P301L-TAU(p R5 mice).Human neurons expressing the longest TAU isoform(2N4R)with the P301L mutation showed increased TAU phosphorylation at the AT8,but not the p-Ser-262 epitope,and increased polyglutamylation and acetylation of microtubules compared with endogenous TAU-expressing neurons.P301L-TAU showed pronounced somatodendritic presence,but also successful axonal enrichment and a similar axodendritic distribution comparable to exogenously expressed 2N4R-wildtype-TAU.P301L-TAU-expressing hippocampal neurons in transgenic mice showed prominent missorting and tauopathy-typical AT8-phosphorylation of TAU and increased polyglutamylation,but reduced acetylation,of microtubules compared with non-transgenic *** sum,P301L-TAU results in changes in micr

关键词： human induced pluripotent stem cell microtubules P301L pR5 mice TAU tauopathy tubulin code

Improving the haploidization toolbox:Maternal factors take the stage

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Molecular Plant 2023年第4期16卷 651-653页

作者： Maria Flores-Tornero Helena Sapeta Jorg d.becker Instituto de Tecnologia Quimica e Biologica Universidade Nova de LisboaAv.daRepublica2780-157OeirasPortugal

Plant breeders have a great interest in obtaining plants that are genetically homogeneous as quickly as possible,with high efficiency and at low *** approaches,including selfing or backcrossing,cannot provide *** technologies,however,offer a solution and are therefore the subject of intensive research,in particular during the last decade.

关键词： breed homogeneous quickly

The Properties and Applications of MoS_2/Ti Composite Coatings in dry Cutting and Forming

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材料热处理学报 2004年第05B期25卷 894-898页

作者： XiaolingZhang HailinSun d.becker S.Wagner d.G.Teer TeerCoatingsLtd. WestStineHouseBerryHillIndustrialEstateDroitwichWorcestershireUK InstituteforMetalFormingTechnology stuttgartUniversityStuttgartGermany

MoSi/Ti composite coatings (MoST?), have been deposited by a closed-field unbalanced magnetron sputter ion-plating system. The high ion-current densities at low substrate bias voltage of the system ensures that the MoST? coatings are characteristic of dense coherent structures, with high adhesion (critical scratch load above 120 N), high load bearing capacity up to 5 GPa., low friction (0.02-0.1), high wear resistance (less than 4xlO"17 m3/N m) even at a relative humidity of 40%, and high temperature resistance up to 350 °C. The successful applications of MoST? coatings in large- scale productions for dry cutting and forming, with increasing lifetime and productivity are presented.

关键词： MoS2/Ti复合涂层干切割低摩擦力磁控管溅射

质粒dNA物理形态和其它因素对获得可育转基因小麦的影响

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实验生物学报 2001年第3期34卷 183-189页

作者：徐子勤 d.becker H.Lrz 西北大学生物技术省重点实验室西安710069 德国汉堡大学普通植物学研究所汉堡d22609

本工作分析了不同形态质粒dNA和未成熟胚高渗透压处理对基因枪小麦转化体系的适用性。高渗处理对瞬间表达和转基因小麦的再生均有明显的促进作用。轰击之前对质粒dNA进行变性处理导致瞬间表达反应大幅度下降,但稳定转化频率(指从100个... 详细信息

本工作分析了不同形态质粒dNA和未成熟胚高渗透压处理对基因枪小麦转化体系的适用性。高渗处理对瞬间表达和转基因小麦的再生均有明显的促进作用。轰击之前对质粒dNA进行变性处理导致瞬间表达反应大幅度下降,但稳定转化频率(指从100个轰击未成熟胚得到的再生可育转基因植株数)与双链dNA相差不大。使用单链质粒dNA、线性双链质粒dNA和环状双链质粒dNA均可以得到转基因小麦植株。迄今已得到26个不同的转基因冬小麦株系和4个不同的转基因春小麦株系。这些转基因小麦大多数已产生种子,几个春小麦株系已获第二代种子。

关键词：转基因小麦质粒dNA 物理形态稳定转化育性

通过卡那霉素选择体系再生可育小麦转基因植株

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自然科学进展（国家重点实验室通讯） 2001年第5期11卷 486-491页

作者：徐子勤 d.becker H.Lrz 西北大学生物技术省重点实验室 Institut fur Allgemeine Botanik Universita HamburgHamburg D-22609Germany

使用卡那霉素作为选择剂建立起一套有效的小麦转化体系,预培养3d的未成熟胚在基因枪轰击前后进行高渗处理,轰击后于26℃、黑暗条件培养2周,然后用150mg/L硫酸卡那霉素选择培养2周,再转至含150mg/L硫酸卡那霉素的再生培养基进行光照培养... 详细信息

使用卡那霉素作为选择剂建立起一套有效的小麦转化体系,预培养3d的未成熟胚在基因枪轰击前后进行高渗处理,轰击后于26℃、黑暗条件培养2周,然后用150mg/L硫酸卡那霉素选择培养2周,再转至含150mg/L硫酸卡那霉素的再生培养基进行光照培养。再生植株在温室中长出2～3片新叶后用2.5％硫酸卡那霉素水溶液喷洒;4周内,非转化体完全白化并枯萎,而转基因植株生长正常,用水稻Actinl启动子控制nptⅡ基因,卡那霉素选择体系的平均转化频率达到2.6％,高渗处理明显促进瞬间表达效果和稳定转化频率。目前已得到23个转基因小麦株系,大多数已经产生种子。Southern分析表明这些转基因株系均整合有不同拷贝的nptⅡ基因。

关键词：卡那霉素选择预培养高渗处理可育转基因小麦基因枪

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Molecular Plant 2012年第4期5卷 951-954页

作者： Giuseppe Ortu Raffaella Balestrini Patricia A. Pereira Jorg d. becker Helge Kuste Paola Bonfante department of Life Science and Systems Biology University of Torino Italy Istituto per la Protezione delle Piante--CNR Torino Italy Instituto Gulbenkian de Ciencia 2780-156 Oeiras Portugal Institut for Pflanzengenetik Leibniz Universitat Hannover Herrenhauser Str. 2 D-30419 Hannover Germany

dear Editor, Phytohormones are essential regulators of plant develop- ment, but their role in the signaling processes between plants and fungi during arbuscular mycorrhizal （AM） establishment is far from being understood （Ludwig-M011er, 2010）. AM coloni- zation leads to extensive effects on host metabolism, as revealed by transcriptome studies of AM plants （Hogekamp et al., 2011）. Some genes have been specified as an AM core set, since they are mycorrhizal-responsive, irrespective of the identity of the plant,

关键词： AM真菌植物激素相关基因早期阶段调节过程生物合成相互作用赤霉素

构词法对无意识加工的影响——中德数字启动效应的比较研究

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心理科学 1993年第1期16卷 8-14页

作者：马红骊方芸秋 G.Luer U.Laβ d.becker R.Heuermann 华东师范大学心理系 Georg August Universitat GottingenGermany

本文采用客观觉察阈值作为启动刺激呈现时间、以数字(阿拉伯数字)和数词(以中、德两种文字写出的数词)为刺激比较研究了在掩蔽刺激条件下中国与德国被试的启动效应.结果表明:1)中国被试的启动效应显著,在德国被试中未发现显著的启动效应... 详细信息

本文采用客观觉察阈值作为启动刺激呈现时间、以数字(阿拉伯数字)和数词(以中、德两种文字写出的数词)为刺激比较研究了在掩蔽刺激条件下中国与德国被试的启动效应.结果表明:1)中国被试的启动效应显著,在德国被试中未发现显著的启动效应;2)除数字—数字刺激组外,中国被试的启动效应显著地大于德国被试;3)中国被试的数词—数字刺激组比其他三个刺激组产生的启动效应大。我们从无意识加工特征、构词法的差异以及并行—连续加工理论的观点对上述结果分别进行了讨论.

关键词：构词法无意识加工启动效应

Structure and Function of TPC1 Vacuole SV Channel Gains Shape

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Molecular Plant 2018年第6期11卷 764-775页

作者： Rainer Hedrich Thomas d. Mueller dirk becker Irene Marten Institute for Molecular Plant Physiology and Biophysics University of Wurzburg Julius-yon-Sachs Platz 2 97082 Wurzburg Germany

Plants and animals in endosomes operate TPCI/SV-type cation channels. All plants harbor at least one TPC1 gene. Although the encoded SV channel was firstly discovered in the plant vacuole membrane two decades ago, its biological function has remained enigmatic. Recently, the structure of a plant TPC1/SV channel protein was determined. Insights into the 3d topology has now guided site-directed mutation ap- proaches, enabling structure-function analyses of TPC1/SV channels to shed new light on earlier findings. Fou2 plants carrying a hyperactive mutant form of TPC1 develop wounding stress phenotypes. Recent studies with fou2 and mutants that lack functional TPC1 have revealed atypical features in local and long-distance stress signaling, providing new access to the previously mysterious biology of this vacuolar cation channel type in planta.

关键词： Ca^2+ sensors TPC1/SV channel vacuole membrane voltage voltage sensor

Perceived Adverse Effects of Separating Government Institutions for disaster Risk Reduction and Climate Change Adaptation Within the Southern African development Community Member States

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International Journal of disaster Risk Science 2021年第1期12卷 1-12页

作者： Livhuwani d.Nemakonde dewald Van Niekerk Per becker Sizwile Khoza African Centre for disaster Studies Unit for Environmental Sciences and ManagementNorth-West UniversityPotchefstroom 2522South Africa Lund University Centre for Risk Assessment and Management(LUCRAM) Lund University22100 LundSweden

Integration of disaster risk reduction(dRR)and climate change adaptation(CCA)is widely recognized as a solution for reducing the risk and impacts of ***,successful integration seems elusive,and the two goals continue to function in isolation and in *** article provides empirical insights into the perceived effects of separating government institutions for dRR and CCA within the Southern African development Community member states.A mixed method research design was applied to the study.A total of 40 respondents from Botswana,Eswatini(until April 2018 Swaziland),Madagascar,Malawi,Namibia,South Africa,Tanzania,Zambia,and Zimbabwe participated in face-to-face interviews or an online *** major effects of separating the organizations for dRR and CCA that impede efforts to reduce disaster risk coherently were identified:duplication of services,polarization of interventions,incoherent policies,competition for the same resources,and territorial *** the continued fragmentation of institutions for dRR and CCA,highlighting these effects is important to emphasize the need for integrated approaches towards the reduction of disaster risk.

关键词： Climate change adaptation disaster risk reduction Southern African development Community Sustainable development