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Two Highly Variable Vpr<sub>84</sub>and Vpr<sub>85</sub>Residues within the HIV-1-Vpr C-Terminal Protein Transduction Domain Control Transductionnal Activity and Define a Clade Specific Polymorphism

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World Journal of AIDS 2014年第2期4卷 148-155页

作者： Jean-Herve Colle Thiery Rose christine rouzioux Alphonse Garcia EA 3620 Universite Paris Descartes AP-HP Laboratoire de Virologie Hopital Necker Paris France Laboratoire E3 Phosphatases Unite Signalisation Moleculaire et Activation Cellulaire Institut Pasteur Paris France Unite d’Immunogenetique Cellulaire Institut Pasteur Paris France Unite de Biologie des Populations Lymphocytaires Institut Pasteur Paris France

The virally encoded HIV-1 viral protein R (VPR) is a multifunctional factor that is required for induced HIV-1 pathogenesis. VPR is also a cell-penetrating protein found in biological fluids from HIV-1 infected individuals. In this regard, we previously published that the C-terminal VPR77-92 sequence from HIV-1 89.6, but not from pNL4.3 strain, is a new pro-apoptotic and protein transduction domain (PTD). Here we report on a sequence analysis of VPR77-92 domain using the Los Alamos HIV-1 sequence database. The analysis showed that the two residues of the domain VPR84 and VPR85 are highly variable and differently biased in HIV-1 clade B and HIV-1 clade C. Furthermore, when Jurkat lymphoblastoid cells or PBMC were incubated with chemically synthesized peptides containing distinct VPR77-92 C-terminal sequences from clades B or C, we found that a clade-dependent polymorphism in VPR84 and VPR85 residues controlled the transducing activity of the C-terminal HIV-1 VPR77-92 domain. Together our data indicate that clade-dependent polymorphism in the VPR84 and VPR85?residues defines the transducing properties mediated by the C-terminal domain of HIV-1 VPR. Identification of this VPR polymorphism suggests new approaches to understand the HIV-1 biology and/or pathogenesis.

关键词： VPR HIV-1 Subtypes Polymorphism Transduction

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Evaluation of HIV Viral Load Activity at the Bacteriology-Virology Laboratory in the University Hospital Center of Brazzaville

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Open Journal of Medical Microbiology 2019年第4期9卷 178-185页

作者： Esther Nina Ontsira Ngoyi Tanguy Mieret Roland Bienvenu Ossibi Ibara Axel Aloumba Géril Obili Sekangue Armel Claude Itoua Irène Laure Opfou Christian Diamant Mossoro-Kpinde Hervé Léon Iloki Bertrand Dupont christine rouzioux Bacteriology-Virology Laboratory of the Teaching Hospital Center (CHU) Brazzaville Congo Health Sciences Faculty of MarienNgouabi University Brazzaville Congo National Laboratory of Clinical Biology and Public Health Ministry of Public Health Central African Republic and University of Bangui Faculty of Health sciences Central African Republic Bangui Central African Republic Infectious Diseases Department of the Teaching Hospital Center (CHU) Brazzaville Congo Gynecology and Obstetrical Department of the Teaching Hospital Center (CHU) Brazzaville Congo Infectious Diseases and Tropical Department Neckerteaching Hospital Center Paris France Virology Laboratory of the Necker Teaching Hospital Center Paris Descartes University Paris France

Introduction: The bacteriology-virology laboratory of the teaching university hospital of Brazzaville, was equipped with a real-time PCR device like Miniopticon (Biorad? , France). The aim of this work was to do an evaluation of the HIV viral load activity, with a view to proposing some recommendations. Material and methods: Retrospective study, January 2013 to March 2015, in patients on first line ARV three-therapy, pre-inclusion therapy checkup in HIV positive patients, but again screening after sexual abuse in women or accident of exposure (AES). A blood sample on EDTA tube was made and RNA extraction with Qiagen kit. Ultrasensitive HIV-RNA quantification was performed using the Generic HIV real-time PCR assay (Biocentric?, Bandol, France). Results: 126 patients were included. The mean age was 37.63 years +/- 10.43 years, sex ratio F/H = 2.3. The HIV viral load was detectable in 94 cases (74.6%). Concerning patients with detectable viral load (copies/ml): 403 to 996 in 35 cases (37.23%), 1411 to 1812 in 41 cases (43.62%) and >1814 in 5 cases (5.32%) (therapeutic failure). Conclusion: This work reports success in the setting up of the molecular biology unit. Procedures that implement information and education actions on the risks associated with AES must be disclosed.

关键词： Viral Load HIV Miniopticon CHU Brazzaville

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