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Endothelin increases the proliferation of rat olfactory mucosa cells

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Neural Regeneration Research 2020年第2期15卷 352-360页

作者： Bertrand Bryche Audrey Saint-Albin Claire le Poupon Schlegel christine Baly Patrice Congar Nicolas Meunier Neurobiologie de l'olfaction Institut National de Recherche Agronomique (INRA)Université Paris-SaclayJouy-en-JosasFrance Université de Versailles Saint-Quentin en Yvelines Département de BiologieVersaillesFrance

The olfactory mucosa holds olfactory sensory neurons directly in contact with an aggressive environment. In order to maintain its integrity, it is one of the few neural zones which are continuously renewed during the whole animal life. Among several factors regulating this renewal, endothelin acts as an anti-apoptotic factor in the rat olfactory epithelium. In the present study, we explored whether endothelin could also act as a proliferative factor. Using primary culture of the olfactory mucosa, we found that an early treatment with endothelin increased its growth. Consistently, a treatment with a mixture of BQ123 and BQ788(endothelin receptor antagonists) decreased the primary culture growth without affecting the cellular death level. We then used combined approaches of calcium imaging, reverse transcriptase-quantitative polymerase chain reaction and protein level measurements to show that endothelin was locally synthetized by the primary culture until it reached confluency. Furthermore, in vivo intranasal instillation of endothelin receptor antagonists led to a decrease of olfactory mucosa cell expressing proliferating cell nuclear antigen(PCNA), a marker of proliferation. Only short-term treatment reduced the PCNA level in the olfactory mucosa cells. When the treatment was prolonged, the PCNA level was not statistically affected but the expression level of endothelin was increased. Overall, our results show that endothelin plays a proliferative role in the olfactory mucosa and that its level is dynamically regulated. This study was approved by the Comité d’éthique en expérimentation animale COMETHEA(COMETHEA C2 EA-45;protocol approval #12-058) on November 28, 2012.

关键词： autocrine factor cell culture cellular dynamics endothelin olfaction olfactory basal cells olfactory epithelium olfactory mucosa primary culture

A self-stabilised walking gait for humanoid robots based on the essential model with internal states

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IET Cyber-Systems and Robotics 2022年第4期4卷 283-297页

作者： Qiuyue Luo christine Chevallereau Yongsheng Ou Jianxin Pang Victor De-león-Gómez Yannick Aoustin Shenzhen Institutes of Advanced Technology Chinese Academy of SciencesShenzhenChina Research Institute of UBTech Robotics ShenzhenChina Laboratoire des Sciences du Numerique de Nantes(LS2N) CNRSCentrale NantesNantes UniversitéNantesFrance FCA at the Univerdidad Autónoma de Coahuila TorreónMéxico

Walking stability is one of the key issues for humanoid robots.A self-stabilised walking gait for a full dynamic model of humanoid robots is *** simplified models,that is,the linear inverted pendulum model and variable-length inverted pendulum model,self-stabilisation of walking gait can be obtained if virtual constraints are properly *** result is extended to the full dynamic model of humanoid robots by using an essential dynamic model,which is developed based on the zero dynamics *** the proposed method,a robust stable walking for a humanoid robot is achieved by adjusting the step timing and landing position of the swing foot automatically,following its intrinsic dynamic *** exempts the robot from the time-consuming high-level control approaches,especially when a full dynamic model is *** different walking patterns/features(i.e.,the swing foot motion,the vertical centre of mass motion,the switching manifold configuration,etc.)affect the stability of the walking gait is *** are conducted on robots Romeo and TALOS to support the results.

关键词： dynamic stability humanoid and bipedal locomotion passive walking

Selection of a cut-off for high-and low-methane producers using a spot-methane breath test:results from a large north American dataset of hydrogen,methane and carbon dioxide measurements in breath

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Gastroenterology Report 2017年第3期5卷 193-199,I0001,I0002页

作者： Klaus Gottlieb Chenxiong le Vince Wacher Joe Sliman christine Cruz Tyler Porter Stephen Carter Synthetic Biologics Inc. RockvilleMarylandUSA Commonwealth Laboratories LLC SalemMassachusettsUSA

Background:levels of breath methane,together with breath hydrogen,are determined by means of repeated collections of both,following ingestion of a carbohydrate substrate,at 15–20 minutes intervals,until 10 samples have been *** frequent sampling is required to capture a rise of hydrogen emissions,which typically occur later in the test:in contrast,methane levels are typically elevated at *** methane emissions represent the principal objective of the test,a spot methane test(i.e.a single-time-point sample taken after an overnight fast without administration of substrate)may be ***:We analysed 10-sample lactulose breath test data from 11674 consecutive unique subjects who submitted samples to Commonwealth Laboratories(Salem,MA,USA)from sites in all of the states of the USA over a one-year *** North American Consensus(NAC)guidelines criteria for breath testing served as a reference ***:The overall prevalence of methane-positive subjects(by NAC criteria)was 20.4%,based on corrected methane results,and 18.9% based on raw *** our USA dataset,the optimal cut-off level to maximize sensitivity and specificity was4ppm CH4,94.5%[confidence interval(CI):93.5–95.4%]and 95.0%(CI:94.6–95.5%),*** use of a correction factor(CF)(5%CO_(2) as numerator)led to reclassifications CH4-high to CH_(4)-low in 0.7% and CH_(4)-low to CH_(4)-high in 2.1%.Conclusions:A cut-off value for methane at baseline of either≥4 ppm,as in our USA dataset,or≥5 ppm,as described in a single institution study,are both highly accurate in identifying subjects at baseline that would be diagnosed as‘methanepositive’in a 10-sample lactulose breath test for small intestinal bacterial overgrowth.

关键词： breath tests spot-methane breath test diagnostic test small intestinal bacterial overgrowth

Mechanism of friction reduction of unsaturated fatty acids as additives in diesel fuels

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Friction 2013年第3期1卷 252-258页

作者： Jean Michel MARTIN christine MATTA Maria-Isabel De Barros BOUCHET Cyrielle FOREST Thierry le MOGNE Thomas DUBOIS Michael MAZARIN Laboratory of Tribology and System Dynamics Ecole Centrale de Lyon69134 EcullyFrance TOTAL CReSBP2269360 Solaize CedexFrance

This paper investigates the effect of unsaturated fatty acid additives on the lubricating properties of a biodiesel,composed of a blend of fatty acid methyl esters(FAMEs)and a conventional diesel *** presence of fatty acids as additives for biodiesel improves their lubricating properties at elevated temperature *** formation of a protective lubricating tribochemical film on the steel contact surfaces has been *** temperature conditions and possibly oxygen are needed to form this film that reduces friction and limits *** analytical tools have revealed the insulating nature of the formed tribofilm and its chemical ***-induced reticulation is thought to play a major role in the growth of this insulating film.

关键词： biodiesel low friction fatty acids tribochemistry surface analysis

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Gastroenterology Report 2018年第3期6卷 242-242,I0003页

Gastroenterol Rep(Oxf).2017 doi:10.1093/gastro/*** authors wish to inform the reader that any references to‘confidential interval’throughout the paper have been corrected to‘confidence interval’.The paper has now ... 详细信息

关键词： methane dioxide corrected

Mineral fibres and environmental monitoring:A comparison of different analytical strategies in New Caledonia

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Geoscience Frontiers 2020年第1期11卷 189-202页

作者： Jasmine Rita Petriglieri christine Laporte-Magoni Peggy Gunkel-Grillon Mario Tribaudino Danilo Bersani Orietta Sala Monika le Mestre Ruggero Vigliaturo Nicola Bursi Gandolfi Emma Salvioli-Mariani Institute of Exact and Applied Sciences Universitéde la Nouvelle CalédonieCampus de NouvilleBP R498851Nouméa CedexNew CaledoniaFrance Department of Chemistry Life Sciences and Environmental SustainabilityUniversity of ParmaParco Area delle Scienze 157/AI-43124 ParmaItaly Department of Mathematical Physical and Computer SciencesUniversity of ParmaParco Area delle Scienze 7/AI-43124 ParmaItaly Arpae Emilia Romagna Sezione Provinciale di Reggio EmiliaCentro Regionale AmiantoVia Amendola 2I-42122Reggio EmiliaItaly Department of Earth and Environmental Science University of Pennsylvania240 S.33rd StreetHayden HallPhiladelphiaPA 19104-6316USA Department of Chemical and Geological Sciences University of Modena and Reggio EmiliaVia Università4I-41125ModenaItaly

Covered by ultrabasic units for more than a third of its surface,the New Caledonia(South West Pacific)is one of the largest world producers of Ni-ore from lateritic *** all outcrops of geological units and open mines contain serpentine and amphibole,also as asbestos *** this geological context,in which weathering processes had a great contribution in the production and dispersion of mineral fibres into the environment,the development of a routinely analytical strategy,able to discriminate an asbestiform fibre from a non-harmful particle,is a pivotal ***,the acquisition of all these parameters is necessary for determining the risk associated to fibres exposition.A multidisciplinary routinely approach,based on the use of complementary simply-to-use but reliable analytical methods is the only possible *** addition,the instrumental apparatus must be easily transportable on the field,directly on the mining *** employment of specialized tools such as Polarized Light Microscopy associated to Dispersion Staining method(PLM/DS)and portable Raman spectroscopy for identification of environmental asbestos,are proved extremely effective in the improvement of the performance and rapidity of data acquisition and *** PLM/DS and handheld Raman devices confirmed to be discriminant in the detection and characterization of asbestos fibres for both serpentine and ***,these techniques proved extremely effective even in the presence of strongly fibrous and altered samples.

关键词： Asbestos Fibrous antigorite In situ monitoring Dispersion staining Portable Raman New Caledonia

Closed-loop superconducting materials discovery

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npj Computational Materials 2023年第1期9卷 471-478页

作者： Elizabeth A.Pogue Alexander New Kyle McElroy Nam Q.le Michael J.Pekala Ian McCue Eddie Gienger Janna Domenico Elizabeth Hedrick Tyrel M.McQueen Brandon Wilfong christine D.Piatko Christopher R.Ratto Andrew lennon christine Chung Timothy Montalbano Gregory Bassen Christopher D.Stiles Research and Exploratory Development Department Johns Hopkins University Applied Physics Laboratory11100 Johns Hopkins RoadLaurel 20723 MDUSA Department of Materials Science and Engineering Northwestern University2220 Campus DriveEvanston 60208 ILUSA Department of Materials Science and Engineering Johns Hopkins University3400 N.Charles StreetBaltimore 21218 MDUSA Department of Chemistry Johns Hopkins University3400 N.Charles StreetBaltimore 21218 MDUSA Institute for Quantum Matter William H.Miller III Department of Physics and AstronomyJohns Hopkins University3400 N.Charles StreetBaltimore 21218 MDUSA

Discovery of novel materials is slow but necessary for societal ***,we demonstrate a closed-loop machine learning(ML)approach to rapidly explore a large materials search space,accelerating the intentional discovery of superconducting *** experimentally validating the results of the ML-generated superconductivity predictions and feeding those data back into the ML model to refine,we demonstrate that success rates for superconductor discovery can be more than *** four closed-loop cycles,we report discovery of a superconductor in the Zr-In-Ni system,re-discovery of five superconductors unknown in the training datasets,and identification of two additional phase diagrams of interest for new superconducting *** work demonstrates the critical role experimental feedback provides in ML-driven discovery,and provides a blueprint for how to accelerate materials progress.

关键词： discovery materials loop

Building a cluster of NLR genes conferring resistance to pests and pathogens: the story of the Vat gene cluster in cucurbits

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Horticulture Research 2021年第1期8卷 899-910页

作者： Veronique Chovelon Rafael Feriche-Linares Guillaume Barreau Joel Chadoeuf Caroline Callot Veronique Gautier Marie-christine le Paslier Aurelie Berad Patricia Faivre-Rampant Jacques Lagnel Nathalie Boissot INRAE GAFL84143 MontfavetFrance INRAE CNRGV31326 Castanet-TolosanFrance INRAE GDEC-Gentyane Plateform63000 Clermont-FerrandFrance Universite Paris-Saclay INRAEEPGV91000 Evry-CourcouronnesFrance

Most molecularly characterized plant resistance genes(R genes)belong to the nucleotide-binding-site-leucine-rich-repeat(NLR)receptor family and are prone to duplication and transposition with high sequence *** this family,the Vat gene in melon is one of the few R genes known for conferring resistance to insect,i.e.,Aphis gossypii,but it has been misassembled and/or mispredicted in the whole genomes of *** examined 14 genomic regions(about 400 kb)derived from long-read assemblies spanning Vat-related genes in Cucumis melo,Cucumis sativus,Citrullus lanatus,Benincasa hispida,Cucurbita argyrosperma,and Momordica *** built the phylogeny of those *** the paleohistory of the Vat gene cluster,we revealed a step by step process beginning from a common ancestry in cucurbits older than 50 *** highlighted Vat exclusively in the Cucumis genera,which diverged about 20 my *** then focused on melon,evaluating a minimum duplication rate of Vat in 80 wild and cultivated melon lines using generalist primers;our results suggested that duplication started before melon *** phylogeny of 44 Vat-CDS obtained from 21 melon lines revealed gain and loss of leucine-rich-repeat domains along ***,we revealed the high putative recognition scale offered in melon based on a combination of SNPs,number of leucine-rich-repeat domains within each homolog and number of homologs within each cluster that might jointly confer resistance to a large pest and pathogen *** on our findings,we propose possible avenues for breeding programs.

关键词： NLR cluster resistance

Mechano-responsive hydrogel for direct stem cell manufacturing to therapy

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Bioactive Materials 2023年第6期24卷 387-400页

作者： Yufeng Shou Ling Liu Qimin Liu Zhicheng le Khang leng lee Hua Li Xianlei Li Dion Zhanyun Koh Yuwen Wang Tong Ming Liu Zheng Yang Chwee Teck Lim christine Cheung Andy Tay Department of Biomedical Engineering National University of Singapore117583Singapore Institute for Health Innovation&Technology National University of Singapore117599Singapore School of Civil Engineering and Architecture Wuhan University of Technology430070WuhanChina NUS Tissue Engineering Program National University of Singapore117510Singapore lee Kong Chian School of Medicine Nanyang Technological University636921Singapore School of Mechanical and Aerospace Engineering Nanyang Technological University639798Singapore Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research(A*STAR)138648Singapore Department of Orthopaedic Surgery Yong Loo Lin School of MedicineNational University of Singapore119288Singapore Mechanobiology Institute National University of Singapore117411Singapore

Bone marrow-derived mesenchymal stem cell(MSC)is one of the most actively studied cell types due to its regenerative potential and immunomodulatory *** cell expansion methods using 2D tissue culture plates and 2.5D microcarriers in bioreactors can generate large cell numbers,but they compromise stem cell potency and lack mechanical preconditioning to prepare MSC for physiological loading expected in *** overcome these challenges,in this work,we describe a 3D dynamic hydrogel using magneto-stimulation for direct MSC manufacturing to *** our technology,we found that dynamic mechanical stimulation(DMS)enhanced matrix-integrinβ1 interactions which induced MSCs spreading and *** addition,DMS could modulate MSC biofunctions including directing MSC differentiation into specific lineages and boosting paracrine activities(e.g.,growth factor secretion)through YAP nuclear localization and FAK-ERK *** our magnetic hydrogel,complex procedures from MSC manufacturing to final clinical use,can be integrated into one single platform,and we believe this‘all-in-one’technology could offer a paradigm shift to existing standards in MSC therapy.

关键词： Mesenchymal stem cell Dynamic mechanical stimulation Magnetic hydrogel Stem cell manufacturing Cell therapy