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De.e.ion or unde.e.pre.sion of the.Y-chromosome.ge.e. CDY2 and HSFY is associate. with maturation arre.t in Ame.ican me. with nonobstructive.azoospe.mia

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Asian Journal of Andrology 2012年第5期14卷 676-682页

作者： Pe.e. J stahl Anna N Mie.nik Christophe. e.Barbie.i Pe.e. N Schle.e. Darius A Paduch De.artme.t of Urology Weill Cornell Medical CollegeNew York Presbyterian HospitaI-CornellNew YorkNY 10065USA

Maturation arre.t （MA） re.e.s to failure.of ge.m ce.l de.e.opme.t le.ding to clinical nonobstructive.azoospe.mia. Although the.azoospe.mic factor （AZF） re.ion of the.human Y chromosome.is cle.rly implicate. in some.case., thus far ve.y little.is known about which individual Y-chromosome.ge.e. are.important for comple.e.male.ge.m ce.l de.e.opme.t. We.sought to ide.tify single.ge.e. on the.Y chromosome.that may be.implicate. in the.pathoge.e.is of nonobstructive.azoospe.mia associate. with MA in the.Ame.ican population. Ge.otype.phe.otype.analysis of 132 me. with Y-chromosome.microde.e.ions was pe.forme.. Prote.n-coding ge.e. associate. with MA we.e.ide.tifie. by visual analysis of a ge.otype.phe.otype.map. Ge.e. associate. with MA we.e.se.e.te. as those.ge.e. within a se.me.t of the.Y chromosome.that, whe. comple.e.y or partially de.e.e., we.e.always associate. with MA and abse.ce.of re.rie.able.te.ticular spe.m. e.pre.sion of e.ch ide.tifie. ge.e.transcript was the. me.sure. with quantitative.RT-PCR in te.ticular tissue.from se.arate.cohorts of patie.ts with idiopathic MA and obstructive.azoospe.mia. Te. candidate.ge.e. for association with MA we.e.ide.tifie. within an 8.4-Mb se.me.t of the.Y chromosome.ove.lapping the.AZFb re.ion. CDY2and HSFYwe.e.the.only ide.tifie. ge.e. for which diffe.e.ce. in e.pre.sion we.e.obse.ve. be.we.n the.MA and obstructive.azoospe.mia cohorts. Me. with obstructive.azoospe.mia had 12-fold highe. re.ative.e.pre.sion of CDY2transcript （1.33__.0.40 vs. 0.11＋_0.04; P=O.O003） and 16-fold highe. e.pre.sion of HSFYtranscript （0.78__.0.32 vs. 0.05_0.02; P=O.O005） compare. to me. with MA. CDY2 and HSFYwe.e.also unde.e.pre.se. in patie.ts with Se.toli ce.l only syndrome. The.e.data indicate.that CDY2and HSFYare.locate. within a se.me.t of the.Y chromosome.that is important for spe.m maturation, and are.unde.e.pre.se. in te.ticular tissue.de.ive. from me. with MA. The.e.obse.vations sugge.t that impairme.ts in CDY2 or HSFYe.pre.sion could be.impli

关键词： CDY1 prote.n CDY2 prote.n ge.e.ics histology HSFY human make.infe.tility nonobstructive.azoospe.mia spe.mato-ge.e.is spe.m maturation

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Nume.ical Visualization of Gre.se.Flow in a Ge.rbox

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Chine.e.Journal of Me.hanical e.gine.ring 2023年第2期36卷 220-233页

作者： Hua Liu Florian Dangl Thomas Lohne. Karste. stahl School of e.gine.ring&De.ign Department of Mechanical EngineeringTechnical University of MunichGear Research Center(FZG)BoltzmannstraBe1585748 Garching near MunichGermany

Lubricating gre.se. are.wide.y use. in *** ge.r drive. and ge.rboxe. with difficult se.ling *** e.ficie.cy and he.t balance.of gre.se.lubricate. ge.rboxe. de.e.d strongly on the.lubrication me.hanisms channe.ing and circulating,for which the.gre.se.flow is *** computational fluid dynamics ope.s up the.possibility to visualize.and unde.stand the.gre.se.flow in ge.rboxe. in more.*** this study,a single.stage.ge.rbox lubricate. with an NLGI 1-2 gre.se.was mode.e. by the.finite.volume.me.hod to nume.ically inve.tigate.the.fluid *** show that the.rotating ge.rs influe.ce.the.gre.se.sump only locally around the.*** a low gre.se.fill volume.the.rotation of the.ge.rs is wide.y se.arate. from the.gre.se.*** a high gre.se.fill volume.a pronounce. ge.rgre.se.inte.action re.ults in a circulating gre.se.flow around the.*** simulate. gre.se.distributions show good accordance.with high-spe.d came.a re.ordings.

关键词： Lubrication Gre.se Transmission Ge.rbox Computational fluid dynamics

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Challe.ge. in de.e.mination of microscopic de.re. of cle.nline.s in ultra-cle.n ge.r ste.ls

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Journal of Iron and Ste.l Re.e.rch Inte.national 2022年第10期29卷 1583-1600页

作者： D.Fuchs T.Tobie.K.stahl Ge.r Re.e.rch Ce.te.(FZG) Technical University of Munich85748 GarchingGermany

Shot-pe.ne.,case.harde.e. ge.rs can fail in the.tooth root due.to crack initiation be.ow the.surface.of the.ste.l ***,the.crack is initiate. at a non-me.allic inclusion in the.ce.te. of a so-calle. *** failure.type.can le.d to a re.uce. e.durance.fatigue.limit of the.*** is for this re.son that,ove. the.last de.ade.much e.fort has be.n inve.te. by ste.l manufacture.s to re.uce.the.non-me.allic inclusion conte.t of ge.r ste.ls so as to mitigate.or e.e. comple.e.y pre.e.t such crack *** ultra-cle.n ge.r ste.ls we.e.achie.e. by various me.sure. in the.ste.l production ***,as a re.ult,the.re.aining non-me.allic inclusions are.inhomoge.e.usly distribute. in the.ste.l volume.in te.ms of both size.and ***,due.to the.inhomoge.e.ty of ultra-cle.n ste.ls,the.que.tion arose.if the.value. de.ive. according to the.standards are.still re.re.e.tative.of ultra-cle.n ste.l *** re.ults show that the.standards can still be.applie.,but more.e.fort must be.*** de.e.mine.the.de.re. of cle.nline.s,six microse.tions are.curre.tly e.aluate. according to ste.l te.t spe.ification(Se.)1571,me.hod *** is shown that an e.amination of 24 microse.tions starting from size.class 0 se.ms be.e.icial to ge. more.re.iable.and comparable.re.ults of the.de.re. of cle.nline.s of the.e.ultra-cle.n ge.r *** addition,it is shown that a high de.re. of cle.nline.s has be.n achie.e. for all ste.l batche. inve.tigate. with the.me.sure. take. in the.ste.l production proce.s.

关键词： De.re. of cle.nline.s Non-me.allic inclusion e.aluation of inclusion Se. 1571 DIN 50602 Ultrasonic imme.sion te.ting Ultra-cle.n ge.r ste.l Ge.r

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De.re.se. plasma urote.sin Ⅱ le.e.s inve.se.y corre.ate.with e.te.t and se.e.ity of coronary arte.y dise.se

Decreased plasma urotensin Ⅱ levels inversely correlate wit...

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第9届中国南方国际心血管病学术会议

作者： Ce.AR V.BORLONGAN christine e.stahl De.artme.t of Ne.rology Medical College of GeorgiaAugustaGA 30912 USA De.artme.t of Inte.nal Me.icine Dwight D.Eisenhower Army Medical CenterFort GordonGA 30909 USA

＜正＞Obje.tive. The.aims of the.pre.e.t study we.e.to de.e.mine.the.plasma UII le.e.s in various type. of coronary he.rt dise.se.and to clarify how the.plasma UII le.e.s corre.ate.with the.clinical pre.e.tation,e.te.t and se.e.ity of coronary arte.y athe.oscle.osis（CAD）.Me.hods One.hundre. and thre. patie.ts unde.going e.e.tive.diagnostic coronary angiography for prove. or clinical suspe.te. coronary he.rt dise.se.we.e.e.rolle. in this *** e.te.t and se.e.ity of coronary arte.y dise.se.we.e.e.aluate. by ve.se. score.and Ge.sini score.*** UII le.e.s we.e.me.sure. by *** The.plasma UII le.e.s in the.patie.ts with mode.t to se.e.e.coronary ste.osis（3.03±0.34 pg/ml,1.83±0.67 pg/ml） we.e.significantly lowe. than that in subje.ts with normal coronary arte.y（4.80±1.11 pg/ml,P＜0.001）.The.plasma UII le.e.s in patie.ts with coronary he.rt dise.se.we.e.also significantly lowe. than that in patie.ts with insignificant coronary ste.osis（P＜0.001）.Compare. to patie.ts with stable.angina pe.toris,plasma UII le.e.s in patie.ts with acute.coronary syndrome.we.e.significantly de.re.se.（1.89±0.51 pg/ml vs 2.42±0.77 pg/ml,P＜0.001）.Plasma UII le.e.s we.e.found to be.ne.ative.y corre.ate. with the.se.e.ity of coronary arte.y ste.osis（r=- 0.488,P＜0.001）,as we.l as the.ve.se. score.r=-0.408, P＜0.05） in the.patie.ts with *** Significant inve.se.corre.ations e.ist be.we.n the.plasma UII le.e.s,and e.te.t and se.e.ity of coronary arte.y *** findings sugge.t that plasma UII contribute. to the.de.e.opme.t and progre.sion of coronary arte.y ste.osis,and may be.a nove. marke. to pre.ict clinical type.,as we.l as e.te.t and se.e.ity of coronary arte.y dise.se.in the.patie.ts.

关键词： Urote.sinⅡ Coronary arte.y athe.oscle.osis Coronary he.rt dise.se

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Quantitative.Variation in Wate.-Use.e.ficie.cy across Wate. Re.ime. and Its Re.ationship with Circadian, Ve.e.ative. Re.roductive. and Le.f Gas-e.change.Traits

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Mole.ular Plant 2012年第3期5卷 653-668页

作者： christine e. e.wards Bre.t e. e.e.s C. Robe.tson McClung Ping Lou Cynthia We.nig De.artme.t of Botany University of Wyoming Laramie WY 82071 USA Pre.e.t addre.s： USACe.e.DC Environmental Laboratory 3909 Halls Ferry Road Vicksburg MS 39180 USA Program in e.ology University of Wyoming Laramie WY 82071 USA De.artme.t of Biological Scie.ce. Dartmouth College Hanover NH 03755 USA

Drought limits light harve.ting, re.ulting in lowe. plant growth and re.roduction. One.trait important for plant drought re.ponse.is wate.-use.e.ficie.cy （WUe.. We.inve.tigate. （1） how the.joint ge.e.ic archite.ture.of WUe. re.roductive.characte.s, and ve.e.ative.traits change. across drought and we.l-wate.e. conditions, （2） whe.he. traits with distinct de.e.opme.tal base. （e.g. le.f gas e.change.ve.sus re.roduction） diffe.e. in the.e.vironme.tal se.sitivity of the.r ge.e.ic archite.ture. and （3） whe.he. quantitative.variation in circadian pe.iod was re.ate. to drought re.ponse.in Brassica rapa. Ove.all, WUe.incre.se. in drought, primarily be.ause.stomatal conductance. and thus wate. loss, de.line. more.than carbon fixation. Ge.otype. with the.highe.t WUe.in drought e.pre.se. the.lowe.t WUe.in we.l-wate.e. conditions, and had the.large.t ve.e.ative.and floral organs in both tre.tme.ts. Thus, large.change. in WUe.e.able. some.ge.otype. to approach ve.e.ative.and re.roductive.trait optima across e.vironme.ts. The.ge.e.ic archite.ture.diffe.e. for gas-e.change.and ve.e.ative.traits across drought and we.l-wate.e. conditions, but not for floral traits. Corre.ations be.we.n circadian and le.f gas-e.change.traits we.e.significant but did not vary across tre.tme.ts, indicating that circadian pe.iod affe.ts physiological function re.ardle.s of wate. availability. The.e.re.ults sugge.t that WUe.is important for drought tole.ance.in Brassica rapa and that artificial se.e.tion for incre.se. WUe.in drought will not re.ult in maladaptive.e.pre.sion of othe. traits that are.corre.ate. with WUe.

关键词： Brassica rapa drought e.ophysiology floral traits G matrix le.f gas-e.change.traits wate.-use.e.ficie.cy ve.e.ative.traits

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Use.of mode.s in large.are. fore.t surve.s： comparing mode.-assiste., mode.-base. and hybrid e.timation

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Fore.t e.osyste.s 2016年第2期3卷 153-163页

作者： Goran stahl Sve.lana Saare.a Se.astian Schne.l Sore. Holm Johanne. Bre.de.bach Se.n P. He.le. Paul L. Patte.son Ste.n Magnusse. e.ik Nae.se. Ronald e. McRobe.ts Timothy G. Gre.oire De.artme.t of Fore.t Re.ource.Manage.e.t Swedish University of Agricultural Sciences UmeA Sweden. Norwe.ian Institute.for Bioe.onomy Re.e.rch As Norway. USDA Fore.t Se.vice Washington D.C. USA. Canadian Fore.t Se.vice Pacific Forestry Centre British Columbia Canada Norwe.ian Unive.sity of Life.Scie.ce. As Norway. School of Fore.try and Fnvirnnme.tal Studie.. Yale.Unive.sitv New Haven CT USA.

This pape. focuse. on the.use.of mode.s for incre.sing the.pre.ision of e.timators in large.are. fore.t surve.s. It is motivate. by the.incre.sing availability of re.ote.y se.se. data, which facilitate. the.de.e.opme.t of mode.s pre.icting the.variable. of inte.e.t in fore.t surve.s. We.pre.e.t, re.ie. and compare.thre. diffe.e.t e.timation frame.orks whe.e.mode.s play a core.role. mode.-assiste., mode.-base., and hybrid e.timation. The.first two are.we.l known, whe.e.s the.third has only re.e.tly be.n introduce. in fore.t surve.s. Hybrid infe.e.ce.mixe. de.ign- base. and mode.-base. infe.e.ce. since.it re.ie. on a probability sample.of auxiliary data and a mode. pre.icting the.targe. variable.from the.auxiliary *** re.ie. studie. on large.are. fore.t surve.s base. on mode.-assiste., mode.- base., and hybrid e.timation, and discuss advantage. and disadvantage. of the.approache.. We.conclude.that no ge.e.al re.omme.dations can be.made.about whe.he. mode.-assiste., mode.-base., or hybrid e.timation should be.pre.e.re.. The.choice.de.e.ds on the.obje.tive.of the.surve. and the.possibilitie. to acquire.appropriate.fie.d and re.ote.y se.se. data. We.also conclude.that mode.ling approache. can only be.succe.sfully applie. for e.timating targe. variable. such as growing stock volume.or biomass, which are.ade.uate.y re.ate. to commonly available.re.ote.y se.se. data, and thus pure.y fie.d base. surve.s re.ain important for se.e.al important fore.t parame.e.s.

关键词： De.ign-base. infe.e.ce Mode.-assiste. e.timation Mode.-base. infe.e.ce Hybrid infe.e.ce Nationalfore.t inve.tory Re.ote.se.sing Sampling

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运用分子遗传学来指导肥厚型心肌病的诊断与治疗

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中华心血管病杂志 2009年第12期37卷 1063-1068页

作者：王立斌 J · G· Se.dman christine e. Se.dman Massachuse.ts 02115 Department of Genetics Harvard Medical School Howard Hughe. Me.ical Institute

肥厚型心肌病(hype.trophy cardiomyopathy,HCM)是常染色体显性遗传疾病,其特征为在无明显诱因的(如高血压或瓣膜疾病)的心肌肥厚~([1]).在过去的50多年中,有关此疾病的文献报道已数以千计.最早对HCM的描述只局限于该病的病理解剖及组... 详细信息

肥厚型心肌病(hype.trophy cardiomyopathy,HCM)是常染色体显性遗传疾病,其特征为在无明显诱因的(如高血压或瓣膜疾病)的心肌肥厚~([1]).在过去的50多年中,有关此疾病的文献报道已数以千计.最早对HCM的描述只局限于该病的病理解剖及组织学特征~([2-3]).随后,因血液动力学改变而导致多种临床表现的机制得到了进一步的阐述,例如左心室流出道梗阻以及无症状患者的猝死。

关键词：分子遗传学肥厚型心肌病诊断治疗

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Se.e.ce.ce.spe.ific Alte.ation of Hydroge. Pe.oxide.Le.e.s in Arabidopsis thaliana and Oilse.d Rape.Spring Varie.y Brassica napus ***

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Journal of Inte.rative.Plant Biology 2012年第8期54卷 540-554页

作者： Ste.an Bie.e. Le.a Rie.te. Mark stahl Jürge. Franzaring Ulrike.Ze.tgraf ZMBP (Ce.te. for Plant Mole.ular Biology) University of TübingenAuf der Morgenstelle 2872076 Tübingen Germany Institute.for Landscape.and Plant e.ology kologiezentrum 2August-von-Hartmann-Str.370599 StuttgartGermany

In orde. to analyze.the.signaling function of hydroge. pe.oxide.（H202） production in se.e.ce.ce.in more.de.ail, we.manipulate. intrace.lular H202 le.e.s in Arabidopsis thaliala （L.） He.nh by using the.hydroge.- pe.oxide.se.sitive.part of the.e.che.ichia coil transcription re.ulator OxyR, which was dire.te. to the.cytoplasm as we.l as into the.pe.oxisome.. H202 le.e.s we.e.lowe.e. and se.e.ce.ce.was de.aye. in both transge.ic line., but OxyR was found to be.more.e.fe.tive.in the.cytoplasm. To transfe. this knowle.ge.to crop plants, we.analyze. oilse.d rape.plants Brassica napus L. cv. Mozart for H20~ and its scave.ging e.zyme. catalase.（CAT） and ascorbate.pe.oxidase.（APX） during le.f and plant de.e.opme.t. H202 le.e.s we.e.found to incre.se.during bolting and flowe.ing time. but no incre.se.could be.obse.ve. in the.ve.y late.stage. of se.e.ce.ce. With incre.sing H202 le.e.s, CAT and APX activitie. de.line., so it is like.y that similar me.hanisms are.use. in oilse.d rape.and Arabidopsis to control H202 le.e.s. Unde. e.e.ate. CO2 conditions, oilse.d rape.se.e.ce.ce.was acce.e.ate. and coincide. with an e.rlie. incre.se.in H202 le.e.s, indicating that H202 may be.one.of the.signals to inducing se.e.ce.ce.in a broade. range.of Brassicace.e.

关键词： Arabidopsis thaliana Brassica napus e. coil Oxy-RD intrace.lular hydroge. pe.oxide se.e.ce.ce.phe.otype catalase ascorbate.e.oxidase e.e.ate. CO2.

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e.rly pre.nancy in the.horse.re.isite.–doe. e.ce.tion prove.the.rule.

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Journal of Animal Scie.ce.and Biote.hnology 2016年第2期7卷 117-124页

作者： christine Aurich Sve. Budik Artificial Inse.ination and e.bryo Transfe. Vetmeduni ViennaVeterinarplatz 11210 ViennaAustria

e.rly e.uine.pre.nancy share. many fe.ture. with that of more.inte.sive.y asse.se. dome.tic animals spe.ie., but the.e.are.also characte.istic diffe.e.ce.. Some.of those.are.poorly unde.stood. De.ce.t of the.e.uine.conce.tus into the.ute.ine.lume. occurs at day 5 to 6 afte. ovulation but is only possible.whe. the.e.bryo se.re.e. prostaglandin e.. Although mainte.ance.of e.uine.pre.nancy probably involve. se.re.ion of a conce.tus de.ive. anti-lute.lytic factor, this age.t has not be.n ide.tifie.. Rapid growth, conce.tus mobility and pre.e.ce.of an ace.lular capsule.at the.time.of mate.nal re.ognition of pre.nancy, i.e. be.we.n days 12 and 14, are.pre.e.uisite. to avoid pre.nancy loss. Proge.te.one.toge.he. with 5α-pre.nane. is se.re.e. by the.corpus lute.m and induce. the.production of e.dome.rial histotroph which is re.ponsible.for conce.tus nutrition until place.tion. A stable.contact be.we.n the.oute. trophoblast laye. of the.allantochorion and the.luminal e.ithe.ium of the.e.dome.rium is not e.tablishe. be.ore.days 40 to 42 of pre.nancy.

关键词： Conce.tus Horse.Mate.nal re.ognition Pre.nancy

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Pre.ision me.icine.in he.ring loss

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Journal of Ge.e.ics and Ge.omics 2018年第2期45卷 99-109页

作者： Jason R.Rudman christine Me. Sara e.Bre.sle. Susan H.Blanton Xue.Zhong Liu De.artme.t of Otoloryngology University of Miami Miller School of Medicine Miami FL 33136 USA Dr. John T. Macdonald Foundation De.artme.t of Human Ce.e.ics University of Miami Miller School of Medicine Miami FL 33136 USA John P. Hussman Institute.for Human Ge.omics University of Miami Miller School of Medicine Miami FL 33136 USA

Pre.ision me.icine.PM） propose. customize. me.ical care.base. on a patie.t＇s unique.ge.ome. biomarke.s, e.vironme.t and be.aviors. He.ring loss（HL） is the.most common se.sorine.ral disorde. worldwide.and is fre.ue.tly cause. by a single.ge.e.ic mutation. With re.e.t advance. in PM tools such as ge.e.ic se.ue.cing and data analysis, the.fie.d of HL is ide.lly positione. to adopt the.strate.ie. of ***, we.re.ie. curre.t and future.applications of PM in HL as the. re.ate.to the.four core.qualitie. of PM（P4）： pre.ictive. pe.sonalize., patie.t-ce.te.e., and participatory. We.the. introduce.a strate.y for e.fe.tive.incorporation of HL PM into the.de.ign of future.re.e.rch studie., e.e.tronic me.ical re.ords,and clinical practice.to improve.diagnostics, prognostics, and, ultimate.y, individualize. patie.t tre.tme.t. Finally, spe.ific anticipate. e.hical and e.onomic conce.ns in this growing e.a of ge.omics-base. HL tre.tme.t are.discusse.. By inte.rating PM principle. into translational HL re.e.rch and clinical practice. he.ring spe.ialists are.unique.y positione. to e.fe.tive.y tre.t the.he.e.oge.e.us cause. and manife.tations of HL on an individualize. basis.

关键词： Pre.ision me.icine.Pe.sonalize. me.icine.He.ring loss Ge.e.ics

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