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Sequencing of 231 forensic genetic markers using the MiSeq FGxTM forensic genomics system-an evaluation of the assay and software

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Forensic Sciences Research 2018年第2期3卷 111-123页

作者： Christian Hussing christina Huber Rajmonda Bytyci Helle S.Mogensen Niels Morling Claus Bφrsting Section of Forensic Genetics Department of Forensic MedicineFaculty of Health and Medical SciencesUniversity of CopenhagenCopenhagenDenmark

The MiSeq FGx^(TM) Forensic Genomics System types 231 genetic markers in one multiplex polymerase chain reaction (PCR) *** markers include core forensic short tandem repeats (STRs) as well as identity,ancestry and phenotype informative short nucleotide polymorphisms (SNPs).In this work,the MiSeq FGx^(TM) Forensic Genomics System was evaluated by analysing reproducibility,sensitivity,mixture identification and forensic phenotyping capabilities of the ***,the genotype calling of the ForenSeq^(TM) Universal Analysis Software was verified by analysing *** files from the MiSeq FGx^(TM) platform using the softwares STRinNGS and ***,the performance of the MiSeq FGx^(TM) Forensic Genomics System was ***,locus and allele drop-outs were relatively frequent at six loci (two STRs and four human identification SNPs) due to low read depth or skewed heterozygote balances,and the stutter ratios were larger than those observed with conventional STR genotyping *** risk of locus and allele drop-outs increased dramatically when the amount of DNA in the first PCR was lower than 250 ***-person 50∶1 mixtures were identified as mixtures,whereas 100∶1 and 1000∶1 mixtures were not.Y-chromosomal short tandem repeats (Y-STRs) alleles were detected in the 100∶1 and 1000∶1 female/male *** ForenSeq^(TM) Universal Analysis Software provided the data analyst with useful alerts that simplified the analysis of the large number of *** of the alerts were due to user-defined,locus-specific *** results shown here indicated that the default settings should be altered for some ***,recommended changes to the assay and software are discussed.

关键词： Forensic science forensic genetics next generation sequencing short tandem repeats single nucleotide polymorphisms ForenSeq^(TM)DNA Signature Prep Kit MiSeq FGx^(TM)Forensic Genomics System

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Specific bone region localization of osteolytic versus osteoblastic lesions in a patient-derived xenograft model of bone metastatic prostate cancer

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Asian journal of Urology 2016年第4期3卷 229-239页

作者： Takeshi Hirata Seung Chol Park Michelle T.Muldong christina N.Wu Tomonori Yamaguchi Amy Strasner Omer Raheem Hiromi Kumon Robert L.Sah Nicholas A.Cacalano Catriona H.M.jamieson Christopher j.Kane Koichi Masuda Anna A.Kulidjian christina A.M.jamieson Department of Urology Okayama University Graduate School of MedicineDentistry and Pharmaceutical SciencesOkayamaJapan Department of Urology Wonkwang University School of Medicine and HospitalIksanSouth Korea Moores Cancer Center University of CaliforniaSan DiegoLa JollaCAUSA Department of Urology University of CaliforniaSan DiegoLa JollaCAUSA Department of Surgery University of CaliforniaSan DiegoLa JollaCAUSA Department of Medicine University of CaliforniaSan DiegoLa JollaCAUSA Department of Orthopaedic Surgery School of MedicineUniversity of CaliforniaSan DiegoLa JollaCAUSA Department of Bioengineering University of CaliforniaSan DiegoLa JollaCAUSA Department of Radiation Oncology University of California at Los AngelesLos AngelesCAUSA

Objective:Bone metastasis occurs in up to 90%of men with advanced prostate cancer and leads to fractures,severe pain and *** metastases induce a spectrum of types of bone lesions which can respond differently to therapy even within individual prostate cancer ***,the special environment of the bone makes the disease more complicated and incurable.A model in which bone lesions are reproducibly induced that mirrors the complexity seen in patients would be invaluable for pre-clinical testing of novel *** microstructural changes in the femurs of mice implanted with PCSD1,a new patient-derived xenograft from a surgical prostate cancer bone metastasis specimen,were ***:Quantitative micro-computed tomography(micro-CT)and histological analyses were performed to evaluate the effects of direct injection of PCSD1 cells or media alone(Control)into the right femurs of Rag2/gc/male ***:Bone lesions formed only in femurs of mice injected with PCSD1 *** volume(BV)was significantly decreased at the proximal and distal ends of the femurs(p<0.01)whereas BV(p<0.05)and bone shaft diameter(p<0.01)were significantly increased along the femur ***:PCSD1 cells reproducibly induced bone loss leading to osteolytic lesions at the ends of the femur,and,in contrast,induced aberrant bone formation leading to osteoblastic lesions along the femur ***,the interaction of PCSD1 cells with different bone region-specific microenvironments specified the type of bone *** approach can be used to determine if different bone regions support more therapy resistant tumor growth,thus,requiring novel treatments.

关键词： Bone metastatic prostate cancer Patient-derived xenograft microenvironment Microstructural CT Osteolytic lesions Osteoblastic lesions

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Interfacial microstructure and mechanical properties of diffusion-bonded joints of titanium TC4 (Ti-6Al-4V) and Kovar (Fe-29Ni-17Co) alloys

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journal of Iron and Steel Research International 2017年第10期24卷 1023-1031页

作者： Ting-feng Song Xiao-song jiang Zhen-yi Shao De-feng Mo De-gui Zhu Min-hao Zhu christina H.Young Zhi-ping Luo School of Materials Science and Engineering Southwest Jiaotong University Department of Material Engineering Chengdu Technological University Key Laboratory of Infrared Imaging Materials and Detectors Shanghai Institute of Technical PhysicsChinese Academy of Sciences Department of Chemistry and Physics Fayetteville State University

Diffusion bonding is a near net shape forming process that can join dissimilar materials through atomic diffusion under a high pressure at a high *** alloy TC4（Ti-6 Al-4 V）and 4 j29 Kovar alloy（Fe-29 Ni-17 Co）were diffusely bonded by a vacuum hot-press sintering process in the temperature range of 700-850°C and bonding time of 120 min,under a pressure of 34.66 *** microstructures and intermetallic compounds of the diffusion-bonded joints were characterized by optical microscopy,scanning electron microscopy,X-ray diffraction（XRD）and energy dispersive spectroscopy（EDS）.The elemental diffusion across the interface was revealed by electron probe *** properties of joints were investigated by micro Vickers hardness and tensile *** of EDS and XRD indicated that（Fe,Co,Ni）-Ti,TiNi,Ti_2Ni,TiNi_2,Fe_2 Ti,Ti_（17） Mn_3 and Al_6 Ti_（19） were formed at the *** the bonding temperature was raised from 700 to 850°C,the voids of interface were reduced and intermetallic layers were *** tensile strength of joints at 53.5 MPa was recorded by the sintering process at 850°C for 120 *** surface of the joint indicated brittle nature,and failure took place through interface of intermetallic *** on the mechanical properties and microstructure of the diffusion-bonded joints,diffusion mechanisms between Ti-6 Al-4 Vtitanium and Fe-29 Ni-17 Co Kovar alloys were analyzed in terms of elemental diffusion,nucleation and growth of grains,plastic deformation and formation of intermetallic compounds near the interface.

关键词： Titanium alloy Kovar alloy Diffusion-bonded joint Microstructure Mechanical property

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Time-dependent density-functional theory molecular-dynamics study on amorphization of Sc-Sb-Te alloy under optical excitation

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npj Computational Materials 2020年第1期6卷 1376-1380页

作者： Xue-Peng Wang Xian-Bin Li Nian-Ke Chen junhyeok Bang Ryky Nelson christina Ertural Richard Dronskowski Hong-Bo Sun Shengbai Zhang State Key Laboratory of Integrated Optoelectronics College of Electronic Science and EngineeringJilin University130012 ChangchunChina Department of Physics Chungbuk National University28644 ChungbukRepublic of Korea Institute of Inorganic Chemistry RWTH Aachen University52056 AachenGermany Hoffmann Institute of Advanced Materials Shenzhen Polytechnic518055 ShenzhenChina State Key Lab of Precision Measurement Technology and Instruments Department of Precision Instrument Tsinghua Univerwsity100084 BeijingChina Department of Physics Applied Physicsand AstronomyRensselaer Polytechnic InstituteTroyNY 12180USA

Recently,all-optical memory and optical-computation properties of phase-change materials are receiving intensive *** writing/erasing information in these devices is usually achieved by laser pulses,the interaction between the laser and the phase-change materials becomes a key issue for such new *** this work,by a time-dependent density-functional theory molecular-dynamics study,the physics underlying the optical excitation induced amorphization of Sc-Sb-Te is revealed,which goes back to superatom-like Sc-centered structural *** motifs are found to be still robust under the excitation.

关键词： optical excitation theory

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LIV-4:A novel model for predicting transplant-free survival in critically ill cirrhotics

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World journal of Hepatology 2020年第6期12卷 298-311页

作者： christina C Lindenmeyer Gianina Flocco Vedha Sanghi Rocio Lopez Ahyoung j Kim Fadi Niyazi Neal A Mehta Aanchal Kapoor William D Carey Eduardo Mireles-Cabodevila Carlos Romero-Marrero Department of Gastroenterology Hepatology and NutritionCleveland ClinicClevelandOH 44195United States Department of Internal Medicine Cleveland ClinicClevelandOH 44195United States Department of Quantitative Health Sciences Cleveland ClinicClevelandOH 44106United States Department of Critical Care Medicine Cleveland ClinicClevelandOH 44195United States

BACKGROUND Critically ill patients with cirrhosis,particularly those with acute decompensation,have higher mortality rates in the intensive care unit(ICU)than patients without chronic liver *** of short-term mortality is important in order to identify patients at highest risk of *** of the currently available prognostic models have been widely accepted for use in cirrhotic patients in the ICU,perhaps due to complexity of calculation,or lack of universal variables readily available for these *** believe a survival model meeting these requirements can be developed,to guide therapeutic decision-making and contribute to cost-effective healthcare resource *** To identify markers that best identify likelihood of survival and to determine the performance of existing survival *** Consecutive cirrhotic patients admitted to a United States quaternary care center ICU between 2008-2014 were included and comprised the training *** data and clinical laboratory test collected on admission to ICU were *** under the curve receiver operator characteristics(AUROC)analysis was performed to assess the value of various scores in predicting inhospital mortality.A new predictive model,the LIV-4 score,was developed using logistic regression analysis and validated in a cohort of patients admitted to the same institution between *** Of 436 patients,119(27.3%)died in the *** multivariate analysis,a combination of the natural logarithm of the bilirubin,prothrombin time,white blood cell count,and mean arterial pressure was found to most accurately predict in-hospital *** from the regression coefficients of the independent variables,a novel model to predict inpatient mortality was developed(the LIV-4 score)and performed with an AUROC of 0.86,compared to the Model for End-Stage Liver Disease,Chronic Liver Failure-Sequential Organ Failure Assessment,and Royal Free Hospital Score,wh

关键词： Risk stratification Resource allocation Intensive care unit Acute-on-chronic liver failure Modeling Mortality

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Ileo-right hemi-colonic cervical pull-up on a non-supercharged ileocolic arterial pedicle: A technical and case report

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World journal of Gastroenterology 2016年第14期22卷 3869-3874页

作者： Andreas RR Weiss christina Hackl Yorick Soeder Hans j Schlitt Marc-H Dahlke Department of Surgery University Medical Center Regensburg

Esophageal reconstruction can be challenging when stomach and colon are not anatomically intact and their use as esophageal substitutes is therefore limited. Innovative individual approaches are then necessary to restore the intestinal passage. We describe a technique in which a short stump of the right hemicolon and 25 cm of ileum on a long, non-supercharged, fully mobilized ileocolic arterial pedicle were used for esophageal reconstruction to the neck. In this case, a 65 year-old male patient had accidentally indigested hydrochloric acid which caused necrosis of his upper digestive tract. An emergency esophagectomy, gastrectomy, duodenectomy, pancreatectomy and splenectomy had been performed in an outside hospital. A cervical esophagostomy and a biliodigestive anastomosis had been created and a jejunal catheter for enteral feeding had been placed. After the patient had recovered, a reconstruction of his food passage via the left and transverse colon failed for technical reasons due to an intraoperative necrotic demarcation of the colon. Our team then faced the situation that only a short stump of the right hemi-colon was left in situ when the patient was referred to our center. After intensified nutritional therapy, we reconstructed this patient's food passage with the right hemicolonapproach described herein. After treatment of a postoperative pneumonia, the patient was discharged from hospital on the 26 th postoperative day in a good clinical condition on an oral-only diet. In conclusion, individual approaches for long-segment reconstruction of the esophagus can be technically feasible in experienced hands. They do not always require arterial supercharging or free intestinal transplantation.

关键词： Esophageal cancer Esophageal trauma Esophageal reconstruction Gastric pull-up Colonic interposition

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Effectiveness of BNT162b2 and CoronaVac vaccines against omicron in children aged 5 to 11 years

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World journal of Pediatrics 2023年第10期19卷 949-960页

作者： Eduardo A.Oliveira Maria christina L.Oliveira Ana Cristina Simões e Silva Enrico A.Colosimo Robert H.Mak Mariana A.Vasconcelos Ludmila R.Silva Daniella B.Martelli Clara C.Pinhati Hercílio Martelli-júnior Department of Pediatrics Health Sciences Postgraduate ProgramSchool of MedicineFederal University of Minas Gerais(UFMG)R.Engenheiro Amaro Lanari 389/501Belo HorizonteMG30130-100Brazil Department of Statistics Federal University of Minas GeraisBelo HorizonteMGBrazil Department of Pediatrics Rady Children’s HospitalUniversity of CaliforniaLa JollaSan DiegoCAUSA Health Science/Postgraduate Program in Nursing.School of Nursing Federal University of Minas Gerais(UFMG)Belo HorizonteMG30130-100Brazil Health Science/Primary Care Postgraduate Program State University of Montes Claros(Unimontes)Montes ClarosMG39401-089Brazil

Background This study aimed to estimate vaccine effectiveness(VE)against omicron variant infection and severe corona virus disease 2019(COVID-19)in children aged 5–11 years hospitalized with acute respiratory *** A test-negative,case–control analysis was conducted from February 2022 to june *** enrolled 6950 eligible children,including 1102 cases and 5848 *** was calculated after immunization with one and two doses of BNT162b2 or *** outcomes were hospitalization with acute respiratory symptoms and detection of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection and severe *** adjusted odds ratio for the association of prior vaccination and outcomes was used to estimate *** For fully vaccinated children,the overall estimated VE against hospitalization with SARS-CoV-2 infection was 42%[95%confidence interval(CI)26 to 54].VE peaked at 29–42 days(67%,95%CI 40%to 82%)and then declined to 19%(95%CI,−20%to 45%)at 57–120 days after the second *** BNT162b2 vaccine had a similar VE against hospitalization with SARS-CoV-2 infection(45%,95%CI,20 to 61)compared to the CoronaVac vaccine(40%,95%CI,17%to 56%).Among cases,56(5%)children died;53(94.6%)were not fully *** cases,the two-dose schedule effectiveness against ICU admission,need for invasive ventilation,severe illness,and death were 10%(95%CI,−54%–45%),22%(95%CI−70%–68%),12%(95%CI,−62%–52%),and 16%(95%CI,−77%–75%),*** For hospitalized children aged 5–11 years during the omicron-predominant period in Brazil,two doses of both vaccines had moderate effectiveness against hospitalization with acute respiratory symptoms and SARS-CoV-2 infection and offered limited protection against endpoints of COVID-19 severity.

关键词： Children COVID-19 Effectiveness Omicron Vaccine

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A vaccination model for COVID-19 in Gauteng,South Africa

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Infectious Disease Modelling 2022年第3期7卷 333-345页

作者： christina j.Edholm Benjamin Levy Lee Spence Folashade B.Agusto Faraimunashe Chirove C.Williams Chukwu David Goldsman Moatlhodi Kgosimore Innocent Maposa K.A.jane White Suzanne Lenhart Department of Mathematics Scripps CollegeClaremontCAUSA Mathematics Department Fitchburg State UniversityFitchburgMAUSA Department of Mathematics University of TennesseeKnoxvilleTNUSA Department of Ecology and Evolutionary Biology University of KansasLawrenceKSUSA Department of Mathematics and Applied Mathematics University of JohannesburgSouth Africa H.Milton Stewart School of Industrial and Systems Engineering Georgia Institute of TechnologyAtlantaGAUSA Biometry and Mathematics Department Botswana University of Agriculture and Natural ResourcesGaboroneBotswana Division of Epidemiology and Biostatistics School of Public HealthFaculty of Health SciencesUniversity of the WitwatersrandJohannesburgSouth Africa Department of Mathematical Sciences University of BathBathUK

The COVID-19 pandemic provides an opportunity to explore the impact of government mandates on movement restrictions and non-pharmaceutical interventions on a novel infection,and we investigate these strategies in early-stage outbreak *** rate of disease spread in South Africa varied over time as individuals changed behavior in response to the ongoing pandemic and to changing government *** a system of ordinary differential equations,we model the outbreak in the province of Gauteng,assuming that several parameters vary over *** data from the time period before vaccination gives the approximate dates of parameter changes,and those dates are linked to government *** parameters are then estimated from available case data and used to assess the impact of each *** forward in time,possible scenarios give projections involving the implementation of two different vaccines at varying *** results quantify the impact of different government policies and demonstrate how vaccinations can alter infection spread.

关键词： COVID-19 Gauteng South Africa ODE epidemiology Model Vaccination Parameter estimation

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Comparison of ultrasound-measured properties of the common carotid artery to tobacco smoke exposure in a cohort of Indonesian patients

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World journal of Emergency Medicine 2017年第3期8卷 177-183页

作者： Allen R.Yu Bima Hasjim Luke E.Yu Christopher Gabriel Alexander Anshus jonathan B.Lee Michael j. Louthan Esther C.Kim Katrina Lee christina Tse Thomas Keown Shadi Lahham Maili Alvarado Steven Bunch Abdulatif Gari j.Christian Fox School of Medicine University of California Department of Emergency Medicine University of California

BACKGROUND: The purpose of this study was to use point-of-care ultrasound(POCUS) to investigate the relationship between tobacco smoke exposure and the characteristics of the common carotid artery(CCA). The effect of both primary and secondary smoking on CCA properties was ***: We performed a prospective cross-sectional study across 20 primary care clinics in Bandung, West java, Indonesia in july 2016. Point of care ultrasound was performed on a convenience sample of Indonesian patients presenting to clinic. The CCA wall stiffness and carotid intima-media thickness(CIMT) were measured during diastole and systole. These measurements were correlated with smoke exposure and cardiovascular ***: We enrolled 663 patients in the study, with 426 patients enrolled in the smoking category and 237 patients enrolled in the second-hand smoke category. There was an overall positive correlation with the measured lifestyle factors and the ultrasound-measured variables in the group of individuals who smoked. For all variables, age seemed to contribute the most out of all of the lifestyle factors for the positive changes in CIMT and CCA wall ***: Our data yielded correlations between CCA properties and cardiovascular risk, as well as between CIMT and arterial stiffness. We were also able to demonstrate an increase in thickness of the CIMT in patients who have been exposed by tobacco through the use of ultrasound. Further large scale studies comparing patients with multiple cardiac risk factors need to be performed to confi rm the utility of ultrasound fi ndings of cardiovascular disease and stroke.

关键词： Carotid artery thickness Point of care ultrasound Indonesia Intimal thickness

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Prosecution records reveal pangolin trading networks in China, 2014–2019

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Zoological Research 2021年第5期42卷 666-670页

作者： Xiang-Qin Huang Chris Newman christina D.Buesching Mei-Ling Shao Yun-Chun Ye Sha Liu David W.Macdonald Zhao-Min Zhou Key Laboratory of Southwest China Wildlife Resources Conservation(Ministry of Education) China West Normal UniversityNanchongSichuan 637002China Wildlife Conservation Research Unit The Recanati-Kaplan CentreDepartment of ZoologyUniversity of OxfordOxford OX135QLUK Cook’s Lake Farming Forestry and Wildlife Inc.(Ecological Consultancy)Queens CountyNova Scotia B0J 2H0Canada Department of Biology Irving K.Barber Faculty of ScienceThe University of British Columbia/OkanaganKelownaBC V1V 1V7Canada Key Laboratory of Environmental Science and Biodiversity Conservation(Sichuan Province) China West Normal UniversityNanchongSichuan 637002China

In a precautionary response to the current coronavirus(COVID-19)pandemic,China’s Ministries permanently banned eating and trading in terrestrial wild(non-livestock)animals on 24 February 2020,and extensively updated the list of Fauna under Special State Protection(LFSSP)in 2020 and 2021,in which pangolins(Manidae spp.)were upgraded to the highest protection *** 509 pangolin prosecution records from China judgements online prior to these changes(01/01/14–31/12/19),we identified that Guangdong,Guangxi and Yunnan Provinces were hotspots for trade in whole pangolins and their scales.

关键词： trading networks mining

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