检索结果-南通市图书馆

Regular nicotine intake increased tooth movement velocity,osteoclastogenesis and orthodontically induced dental root resorptions in a rat model

引用

International Journal of Oral Science 2017年第3期9卷 174-184页

作者： christian kirschneck michael maurer michael wolf claudia reicheneder peter proff department of orthodontics university medical centre of regensburgRegensburgGermany department of oral and maxillofacial surgery university medical centre of regensburgRegensburgGermany department of orthodontics rheinische friedrich wilhelm university of bonnBonnGermany

Orthodontic forces have been reported to significantly increase nicotine-induced periodontal bone loss. At present, however, it is unknown, which further （side） effects can be expected during orthodontic treatment at a nicotine exposure corresponding to that of an average European smoker. 63 male Fischer344 rats were randomized in three consecutive experiments of 21 animals each （A/B/C） to 3 experimental groups （7 rats, 112/3）. （A） cone-beam-computed tomography （CBCT）; （B） histology/serology; （C） reverse- transcription quantitative real-time polymerase chain reaction （RT-qPCR）/cotinine serology--（1） control; （2） orthodontic tooth movement （OTM） of the first and second upper left molar （NiTi closed coil spring, 0.25 N）; （3） OTM with 1.89 mg-kg- 1 per day s.c. of L（- ）-nicotine. After 14 days of OTM, serum cotinine and IL-6 concentration as well as orthodontically induced inflammatory root resorption （OIIRR）, osteoclast activity （histology）, orthodontic tooth movement velocity （CBCT, within 14 and 28 days of OTM） and relative gene expression of known inflammatory and osteoclast markers were quantified in the dental-periodontal tissue （RT-qPCR）. Animals exposed to nicotine showed significantly heightened serum cotinine and IL-6 levels corresponding to those of regular European smokers. Both the extent of root resorption, osteoclast activity, orthodontic tooth movement and gene expression of inflammatory and osteoclast markers were significantly increased compared to controls with and without OTM under the influence of nicotine. We conclude that apart from increased periodontal bone loss, a progression of dental root resorption and accelerated orthodontic tooth movement are to be anticipated during orthodontic therapy, if nicotine consumption is present. Thus patients should be informed about these risks and the necessity of nicotine abstinence during treatment.

关键词： dental research inbred Fischer344 nicotine orthodontics rats root resorption tooth movement

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The role of mechanotransduction versus hypoxia during simulated orthodontic compressive strain——an in vitro study of human periodontal ligament fibroblasts

引用

International Journal of Oral Science 2019年第4期11卷 315-324页

作者： Niklas Ullrich Agnes Schroder Jonathan Jantsch Gerrit Spanier Peter Proff christian kirschneck University Medical Centre of Regensburg Franz-Josef-Strauβ-Allee 11D-93053 RegensburgGermany Department of Orthodontics University Medical Centre of RegensburgFranz-Josef-Strauβ-Allee 11D-93053 RegensburgGermany Department of Medical Microbiology and Hygiene University Medical Centre of RegensburgFranz-Josef-Strauβ-Allee 11D-93053 RegensburgGermany Department of Cranio-Maxillo-Facial Surgery University Medical Centre of RegensburgFranz-Josef-Strauβ-Allee 11D-93053 RegensburgGermany

During orthodontic tooth movement(OTM)mechanical forces trigger pseudo-inflammatory,osteoclastogenic and remodelling processes in the periodontal ligament(PDL)that are mediated by PDL fibroblasts via the expression of various signalling *** far,it is unknown whether these processes are mainly induced by mechanical cellular deformation(mechanotransduction)or by concomitant hypoxic conditions via the compression of periodontal blood *** primary PDL fibroblasts were randomly seeded in conventional six-well cell culture plates with O2-impermeable polystyrene membranes and in special plates with gas-permeable membranes(Lumox?,Sarstedt),enabling the experimental separation of mechanotransducive and hypoxic effects that occur concomitantly during *** simulate physiological orthodontic compressive forces,PDL fibroblasts were stimulated mechanically at 2 g·cm?2 for 48 h after 24 h of *** quantified the cell viability by MTT assay,gene expression by quantitative real-time polymerase chain reaction(RT-qPCR)and protein expression by western blot/enzyme-linked immunosorbent assays(ELISA).In addition,PDL-fibroblast-mediated osteoclastogenesis(TRAP+cells)was measured in a 72-h coculture with RAW264.7 *** expression of HIF-1α,COX-2,PGE2,VEGF,COL1A2,collagen and ALPL,and the RANKL/OPG ratios at the mRNA/protein levels during PDL-fibroblast-mediated osteoclastogenesis were significantly elevated by mechanical loading irrespective of the oxygen supply,whereas hypoxic conditions had no significant additional *** cellular–molecular mediation of OTM by PDL fibroblasts via the expression of various signalling molecules is expected to be predominantly controlled by the application of force(mechanotransduction),whereas hypoxic effects seem to play only a minor *** the context of OTM,the hypoxic marker HIF-1αdoes not appear to be primarily stabilized by a reduced O2 supply but is rather stabilised mechanically.

关键词： RAW264.7 periodontal mec

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

年龄评估在正畸及全科口腔医学中的应用

引用

中国口腔医学继续教育杂志 2019年第5期22卷 275-281页

作者： christian kirschneck Peter Proff 付肖依(译) 聂琼(审) 不详北京大学口腔医院正畸科北京市海淀区100081

在口腔医学中,如何判断年龄及牙齿和骨骼的生长发育阶段非常重要,特别是在评估牙列的发育障碍及进行正畸治疗设计时。此外,口腔医学在法医学领域也很重要,可以根据牙列情况确定年龄。除了人体测量学和形态学方法之外,还有许多生物化学,... 详细信息

在口腔医学中,如何判断年龄及牙齿和骨骼的生长发育阶段非常重要,特别是在评估牙列的发育障碍及进行正畸治疗设计时。此外,口腔医学在法医学领域也很重要,可以根据牙列情况确定年龄。除了人体测量学和形态学方法之外,还有许多生物化学,组织学,放射学和无放射的成像方法可以用来确定一个人或一个病人的实际年龄、牙龄和骨龄。本文旨在综述目前口腔医学中用于年龄评估的可行方法,既可用于法医学领域又可用于治疗诊断目的,并基于目前实验结果批判性评估不同方法的适应证及其价值。

关键词：人体测量学实际年龄牙龄放射影像学骨龄

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：