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A Small Multifunctional Pentatricopeptide Repeat Protein in the Chloroplast of Chlamydomonas reinhardtii

Molecular Plant 2015年第3期8卷 412-426页

作者： Abdullah Jala christian Schwarz christian Schmitz-Linneweber Olivier Vallon Jorg Nickelsen Alexandra-Viola Bohne Molecular Plant Sciences Ludwig-Maximillians-University Grosshaderner Straβe 2-4 82152 Planegg-Martinsried Germany Molecular Genetics Institute of Biology Humboldt University of Berlin 10115 Berlin Germany UMR7141 CNRS/Université Pierre et Marie Curie Institut de Biologie Physico-Chimique 13 Rue Pierre et Marie Curie 75005 Paris France

Organellar biogenesis is mainly regulated by nucleus-encoded factors, which act on various steps of gene expression including RNA editing, processing, splicing, stabilization, and translation initiation. Among these regulatory factors, pentatricopeptide repeat （PPR） proteins form the largest family of RNA binding proteins, with hundreds of members in flowering plants. In striking contrast, the genome of the unicellular green alga Chlamydomonas reinhardtii encodes only 14 such proteins. In this study, we analyzed PPR7, the smallest and most highly expressed PPR protein in C. reinhardtii. Green fluorescent protein-based localization and gel-filtration analysis revealed that PPR7 forms a part of a high-molecular-weight ribonu- cleoprotein complex in the chloroplast stroma. RIP-chip analysis of PPRT-bound RNAs demonstrated that the protein associates with a diverse set of chloroplast transcripts in vivo, i.e. rrnS, psbH, rpoC2, rbcL, atpA, cemA-atpH, tscA, and atpl-psaJ. Furthermore, the investigation of PPR7 RNAi strains revealed that deple- tion of PPR7 results in a light-sensitive phenotype, accompanied by altered levels of its target RNAs that are compatible with the defects in their maturation or stabilization. PPR7 is thus an unusual type of small multi- functional PPR protein, which interacts, probably in conjunction with other RNA binding proteins, with numerous target RNAs to promote a variety of post-transcriptional events.

关键词： pentatricopeptide repeat protein chloroplast gene expression RNA binding protein RNA maturation,RNA stabilization RIP-chip

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Ecology and eScience

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Ecological Processes 2012年第1期1卷 124-128页

作者： christian Mulder National Institute for Public Health and the Environment(RIVM) Antonie van Leeuwenhoeklaan 9Bilthoven3720 BAThe Netherlands

Comprehending the gifts of ecology Stress ecology,climate change,human well-being,and global sustainability are popular items(Naeem et al.2009).Given all the challenges in a developing world where the global populatio... 详细信息

关键词： ecology global

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Translation and Time： A Memento of the Curvature of the Poststructuralist Plane

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Frontiers of Literary Studies in China-Selected Publications from Chinese Universities 2012年第3期6卷 426-468页

作者： christian Uhl Department for South and East Asian Languages and Cultures Ghent University Ghent 9000 Belgium

Ever since Derrida＇s appropriation of Walter Benjamin＇s ＂The Task of the Translator,＂ and the deconstruction of the traditional notion of translation as an unequivocal communication of meaning, ＂translation＂ has become a powerful conceptual means in many fields of the humanities to underscore the agency of the subalterns in the context of colonialism, imperialism, or globalization, and to emphasize historical contingency over against necessity. As a matter of consequence, however, the question of the historically specific condition of the possibility of such ＂translation＂ has been largely neglected. In this essay I will argue, that a profound understanding of the processes of social and cultural transformation, which have been conceptualized in terms of ＂translation,＂ can not be achieved unless global capitalism and its specific temporal dynamics are taken into consideration more seriously. Finally, this approach will also enable us to re-read Benjamin, and to reassess the significance of his ＂Task＂ for a more powerful critique of a social formation, ＂which produces commodities.＂

关键词： translation culture capital time deconstruction karl Marx Moishe Postone Walter Benjamin

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Ray tracing particle image velocimetry-Challenges in the application to a packed bed

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Particuology 2024年第1期84卷 194-208页

作者： Christin Velten Mirko Ebert christian Lessig katharina Zahringer Lehrstuhl für Strömungsmechanik und Strömungstechnik Otto-von-Guericke-Universität Magdeburg39106MagdeburgGermany Institut für Simulation und Graphik Otto-von-Guericke-Universität Magdeburg39106MagdeburgGermany

Ray tracing Particle Image Velocimetry(RT-PIV)is an optical technique for high resolution velocity measurements in challenging optical systems,such as transparent packed beds,that uses ray tracing to correct for distortions introduced by transparent geometries in the light *** ray tracing based correction is a post processing step applied to the raw PIV particle images before classical PIV *** this study,RT-PIV is performed in the top layer of a body centred cubic(bcc)sphere packing with gaseous flow,where optical access is obtained by the use of transparent N-Bk7 glass balls with a diameter of d=40 ***-PIV introduces new experimental and numerical challenges,for example a limited field of view,illumination difficulties,a very large required depth of field and high sensitivity to geometric parameters used in the ray tracing *** challenges and their implications are the main scope and discussed in the present ***,the validation of the ray tracing reconstruction step is presented and examples for the obtained corrected vector fields in a packed bed are *** results show the strength of the method in reconstructing velocity fields behind transparent spheres that would not have been accessible by optical measurement techniques without the ray tracing correction.

关键词： Ray tracing particle image velocimetry(RT-PIV) Transparent spherical particles Packed bed Distortion correction Gas flow

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GLOBAL WEAk SOLUTIONS FOR A NONLINEAR HYPERBOLIC SYSTEM

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Acta Mathematica Scientia 2020年第5期40卷 1185-1194页

作者： Qingyou SUN Yunguang LU christian kLINGENBERG k.k.Chen Institute for Advanced Studies Hangzhou Normal UniversityHangzhou 311121China Department of Mathematics Wuerzburg UniversityWuerzburg 97070Germany

In this paper, we study the global existence of weak solutions for the Cauchy problem of the nonlinear hyperbolic system of three equations(1.1) with bounded initial data(1.2). When we fix the third variable s, the system about the variables ρ and u is the classical isentropic gas dynamics in Eulerian coordinates with the pressure function P(ρ, s) = ese-1/ρ,which, in general, does not form a bounded invariant region. We introduce a variant of the viscosity argument, and construct the approximate solutions of(1.1) and(1.2) by adding the artificial viscosity to the Riemann invariants system(2.1). When the amplitude of the first two Riemann invariants(w1(x, 0), w2(x, 0)) of system(1.1) is small,(w1(x, 0), w2(x, 0)) are nondecreasing and the third Riemann invariant s(x, 0) is of the bounded total variation, we obtained the necessary estimates and the pointwise convergence of the viscosity solutions by the compensated compactness theory. This is an extension of the results in [1].

关键词： global weak solutions viscosity method compensa ted compac tn ess

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3D-Printed PLA Filaments Reinforced with Nanofibrillated Cellulose

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Journal of Renewable Materials 2020年第7期8卷 759-772页

作者： Matea Perić Robert Putz christian Paulik Wood k Plus-Competence Center for Wood Composites&Wood Chemistry Kompetenzzentrum Holz GmbHLinzA-4040Austria Institute for Chemical Technology of Organic Materials Johannes Kepler University LinzLinz4040Austria

In the current study poly(lactic acid)PLA composites with a 3 wt%and 5 wt%of nanofibrillated cellulose(NFC)were produced by 3D-printing *** enzymatic pretreatment coupled with mechanical fibrillation in a twin screw extruder was used to produce high consistency *** electron microscopy(SEM)equipped with Fibermetric software,FASEP fiber length distribution analysis,Furrier transform infrared spectroscopy(FT-IR),thermogravimetric analysis(TGA),tensile tests,impact tests and differential scanning calorimetry were used to characterize NFC and PLA/NFC *** results of the fiber length and width measurements together with the results of the SEM analysis showed that enzymatic hydrolysis coupled with a twin screw extrusion could effectively reduce the diameter and length of cellulose *** produced NFC consisted of microand nanosized fibers entangled in a characteristic *** on the FT-IR analysis,no new bonds were formed during the enzymatic hydrolysis or fibrillation *** TGA analysis confirmed that produced NFC can be used in hightemperature extrusion processing without NFC *** the PLA/NFC composites preparation the NFC agglomerates were formed,which negatively influenced PLA/NFC composites impact *** slightly improved tensile strength and elastic modulus were reported for all composites when compared to the neat *** elongation at break was not affected by the NFC *** significant differences in thermal stability were detectable among composites nor in comparation with the neat ***,the crystallinity degree of the composite containing 5 wt%NFC was increased in respect to the neat PLA.

关键词： Nanofibrillated cellulose enzymatic hydrolysis twin screw extruder poly(lactic acid) 3D-printing

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Efficient determination of stability lobe diagrams by in-process varying of spindle speed and cutting depth

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Advances in Manufacturing 2018年第3期6卷 272-279页

作者： christian Brecher Prateek Chavan Alexander Epple Laboratory for Machine Tools and Production Engineering(WZL) RWTH Aachen University Campus-Boulevard 3052074 Aachen Germany

The experimental determination of stability lobediagrams （SLDs） in milling can be realized by eithercontinuously varying the spindle speed or by varying thedepth of cut. In this paper, a method for combining boththese methods along with an online chatter detectionalgorithm is proposed for efficient determination of *** accomplish this, communication between the machinecontrol and chatter detection algorithm is established, andthe machine axes are controlled to change the spindle speedor depth of cut. The efficiency of the proposed method isanalyzed in this paper.

关键词： Chatter detection Poincaré Cutting depthvariation Spindle speed

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3D tissue engineering,an emerging technique for pharmaceutical research

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Acta Pharmaceutica Sinica B 2018年第5期8卷 756-766页

作者： Gregory Jensen christian Morrill Yu Huang Department of Biological Engineering Utah State University

Tissue engineering and the tissue engineering model have shown promise in improving methods of drug delivery, drug action, and drug discovery in pharmaceutical research for the attenuation of the central nervous system inflammatory response. Such inflammation contributes to the lack of regenerative ability of neural cells, as well as the temporary and permanent loss of function associated with neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and traumatic brain injury. This review is focused specifically on the recent advances in the tissue engineering model made by altering scaffold biophysical and biochemical properties for use in the treatment of neurodegenerative diseases. A portion of this article will also be spent on the review of recent progress made in extracellular matrix decellularization as a new and innovative scaffold for disease treatment.

关键词： Tissue engineering Decellularization Neurodegenerative disease Drug screening Neuroinflammation 3D cell culture

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A review of synthetic methods for the production of upconverting lanthanide nanoparticles

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Journal of Innovative Optical Health Sciences 2014年第2期7卷 24-34页

作者： christian F.Gainer Marek Romanowski Department of Biomedical Engineering Uniuersity of Arizona Tucson AZ

Upconverting lanthanide nanoparticles overcome many of the problems ssociated with moretraditionally used luminescent contrast agents,such as photobleaching,autofluorescence,cyto-toxicity and *** this reason,they are an atractive choice for biomedical imagingapplications,particularly for imaging in living *** last decade has seen numerousimprovements to these nanocrystals,but a comprehensive guide to the synthesis of upconvertinglanthanide nanoparticles has not yet been *** vary from paper to paper and fromgroup to group,and results vary bet ween research groups for each *** this reason,development of these nanoparticles remains a signicant endeavor for any research groupinterested in joining the *** this review,we look at the varying synthetic methods employedover the last decade and detail methodology for a select few that have been favored in the field.

关键词： Rare earth nanocrystals upconversion imaging luminescence

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Regulation of hepatic blood flow:The hepatic arterial buffer response revisited

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World Journal of Gastroenterology 2010年第48期16卷 6046-6057页

作者： christian Eipel kerstin Abshagen Brigitte Vollmar Institute for Experimental Surgery Medical FacultyUniversity of Rostock

The interest in the liver dates back to ancient times when it was considered to be the seat of life processes. The liver is indeed essential to life,not only due to its complex functions in biosynthesis,metabolism and clearance,but also its dramatic role as the blood volume reservoir. Among parenchymal organs,blood flow to the liver is unique due to the dual supply from the portal vein and the hepatic artery. knowledge of the mutual communication of both the hepatic artery and the portal vein is essential to understand hepatic physiology and pathophysiology. To distinguish the individual importance of each of these inflows in normal and abnormal states is still a challenging task and the subject of on-going research. A central mechanism that controls and allows constancy of hepatic blood flow is the hepatic arterial buffer response. The current paper reviews the relevance of this intimate hepatic blood flow regulatory system in health and disease. We exclusively focus on the endogenous interrelationship between the hepatic arterial and portal venous inflow circuits in liver resection and transplantation,as well as inflammatory and chronic liver diseases. We do not consider the hepatic microvascular anatomy,as this has been the subject of another recent review.

关键词： Hepatic blood flow Hepatic arterial buffer response Liver

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