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*** binding properties of the C-terminal segment of retinol dehydrogenase 8

Annals of Eye Science 2018年第1期 428-428页

作者： AndréHädicke Ana I.A.Coutinho François Otis Mustapha Lhor Line Cantin Manuel Prieto Normand Voyer christian salesse PROTEO and CUO-Recherche Centre de recherche du CHU de Québec-UniversitéLavalHôpital St-SacrementQuébecCanada Centro Química-Física Molecular Instituto Superior TécnicoUniversidade de LisboaLisboaPortugal PROTEO and Dépt.Chimie FacultéSciences et génieUniversitéLavalQuébecCanada

Background:Retinol dehydrogenase 8(RDH8)is a 312-amino acid(aa)protein involved in the visual *** to the outer segment disk membranes of photoreceptors,it reduces all-trans-retinal to all-trans-retinol1 as one of the rate-limiting steps of the visual ***8 is a member of the short-chain dehydrogenase/reductase *** C-terminal segment allows its membrane-anchoring through the postulated presence of an amphipathicα-helix and of 1 to 3 acyl groups at positions 299,302 and *** secondary structure and membrane binding characteristics of RDH8 and its C-terminal segment have not yet been ***:To evaluate the membrane binding of RDH8,the full-length protein(aa 1-312),a truncated form(aa 1-296),its C-terminal segment(aa 281-312 and 297-312)as well as different additional variants of this segment were *** truncated protein binds membranes less efficiently than the full-length ***,the C-terminal segment of RDH8 is essential for the binding and has thus been further *** intrinsic fluorescence of tryptophan residues at positions 289 and 310 of the wild-type C-terminal segment of RDH8 and the mutants W289F,W310F and W310R have thus been used to determine their extent of binding to lipid vesicles and to monitor their local *** lipid vesicles composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine(POPC)or a mixture of POPC and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine(POPS)were used to mimic the phospholipid content of the outer segment disk membranes of ***:An increase in fluorescence intensity and in fluorescence lifetime is observed upon increasing the concentration of lipid *** data allowed calculating values of partition coefficient of the C-terminal segment of RDH8 varying between Kp=1.1 E6 to 1.7 *** is noteworthy that the observation of a more intense shift to lower wavelengths upon membrane binding of the mutant W310R and W310F indicates a deeper inco

关键词： Peptide-membrane binding electrostatic interactions hydrophobic interactions fluorescence spectroscopy

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*** database of clinical data and biological material for research on uveal melanoma

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Annals of Eye Science 2018年第1期 453-453页

作者： Solange Landreville Frédéric Mouriaux Patrick J.Rochette christian salesse Mohib W.Morcos Claudine Bellerive Sylvain Guérin Centre de recherche du CHU de Québec-UniversitéLaval Hôpital du Saint-SacrementQuébecQCCanada Département d’ophtalmologie et d’ORL-CCF Facultéde médecineUniversitéLavalQuébecQCCanada Centre de recherche en organogénèse expérimentale de l’UniversitéLaval/LOEX QuébecQCCanada Département d'ophtalmologie Facultéde médecineUniversitéde Rennes 1RennesFrance Service d’ophtalmologie CHU de RennesRennesFrance

Background:Sharing biological material and clinical data from patients with uveal ***:Uveal melanoma is the most common intraocular malignancy in the adult *** uveal melanoma is primarily a sporadic cancer and familial cases are rare,it is difficult to prevent or detect *** effective treatment of ocular tumors,more than 50%of patients develop incurable liver metastases mainly in the 5-10 years following the detection of the primary *** cancer is relatively rare and the obtained biopsies are very *** 20 samples are taken each year in *** provincial infrastructure is made of biological material from donors with uveal melanoma and a large clinical *** tumor biopsies are used for culturing cell lines and the creation of a DNA/RNA library used for genomic and genetic ***:This infrastructure plays an important role in the achievement of various research programs for a better understanding of genetic and environmental factors involved in the development of melanoma and the spread of *** allows collaboration with other researchers at a provincial,national and international level in order to make progress in basic and clinical research on uveal ***:The biological material and clinical data of this infrastructure are available upon request to VHRN members whose research project was approved by the ethics committee of the institution.

关键词： Uveal melanoma tumor biopsies blood clinical data

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