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Transcriptome Analysis of High-Temperature Stress in Developing Barley Caryopses： Early Stress Responses and Effects on Storage Compound Biosynthesis

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Molecular Plant 2011年第1期4卷 97-115页

作者： Elke Mangelsen Joachim Kilian Klaus Harter christer jansson Dierk Wanke Eva Sundberg Department of Plant Biology and Forest Genetics The Swedish University of Agricultural Sciences (SLU) PO Box 7080 SE-750 07 Uppsala Sweden Center of Plant Molecular Biology (ZMBP) University of Tuebingen Auf der Morgenstelle 1 D-72076 Tvbingen Germany Lawrence Berkeley National Laboratory Earth Sciences Division 1 Cyclotron Rd Berkeley CA 94720 USA

High-temperature stress, like any abiotic stress, impairs the physiology and development of plants, including the stages of seed setting and ripening. We used the Affymetrix 22K Barley1 GeneChip microarray to investigate the response of developing barley （Hordeum vulgare） seeds, termed caryopses, after 0.5, 3, and 6 h of heat stress exposure; 958 induced and 1122 repressed genes exhibited spatial and temporal expression patterns that provide a detailed insight into the caryopses＇ early heat stress responses. Down-regulation of genes related to storage compound biosynthesis and cell growth provides evidence for a rapid impairment of the caryopsis＇ development. Increased levels of sugars and amino acids were indicative for both production of compatible solutes and feedback-induced accumulation of substrates for storage compound biosynthesis. Metadata analysis identified embryo and endosperm as primary locations of heat stress responses, indicating a strong impact of short-term heat stress on central developmental functions of the caryopsis. A comparison with heat stress responses in Arabidopsis shoots and drought stress responses in barley caryopses identified both conserved and presumably heat- and caryopsis-specific stress-responsive genes. Summarized, our data provide an important basis for further investigation of gene functions in order to aid an improved heat tolerance and reduced losses of yield in barley as a model for cereal crops.

关键词： Abiotic/environmental stress gene expression transcriptome analysis seed biology barley caryopsis crops heat shock.

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A Dual-Promoter Gene Orchestrates the Sucrose-Coordinated Synthesis of Starch and Fructan in Barley

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Molecular Plant 2017年第12期10卷 1556-1570页

作者： Yunkai Jin Mingliang Fei Sara Rosenquist Lu Jin Suresh Gohil Corine Sandstrom Helena Olsson Cecilia Persson Anna-Stina Hoglund Gunnel Fransson Ying Ruan Per Aman christer jansson Chunlin Liu Roger Andersson Chuanxin Sun Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization Hunan Agricultural University Changsha 410128 China Department of Plant Biology Uppsala BioCenter Linnean Centre for Plant Biology Swedish University of Agricultural Sciences P.O. Box 7080 75007 UppsalaSweden Key Laboratory of Education Department of Hunan Province on Plant Genetics and Molecular Biology College of Bioscience and BiotechnologyHunan Agricultural University Changsha 410128 China Department of Chemistry and Biotechnology Uppsala BioCenter Swedish University of Agrioultural Scienoes P.O. Box 7015 75007 Uppsala Sweden The Swedish NMR Centre at University of Gothenburg Box 465 405 30 Gothenburg Sweden Department of Molecular Sciences Uppsala BioCenter Swedish University of Agricultural Sciences P.O. Box 7051 750 07 Uppsala Sweden The Environmental Molecular Sciences Laboratory (EMSL) Pacific Northwest National Laboratory P.O. Box 999 K8-93 Richland WA 99352 USA These authors contributed equally to this article.

Sequential carbohydrate synthesis is important for plant survival because it guarantees energy supplies for growth and development during plant ontogeny and reproduction. Starch and fructan are two important carbohydrates in many flowering plants and in human diets. Understanding this coordinated starch and fructan synthesis and unraveling how plants allocate photosynthates and prioritize different carbohydrate synthesis for survival could lead to improvements to cereals in agriculture for the purposes of greater food security and production quality. Here, we report a system from a single gene in barley employing two alternative promoters, one intronic/exonic, to generate two sequence-overlapping but functionally opposing transcription factors, in sensing sucrose, potentially via sucrose/gluco lose 6-phosphate signaling. The system employs an autoregulatory mechanism in perceiving a sucrose- controlled trans activity on one promoter and orchestrating the coordinated starch and fructan synthesis by competitive transcription factor binding on the other promoter. As a case in point for the physiological roles of the system, we have demonstrated that this multitasking system can be exploited in breeding barley with tailored amounts of fructan to produce healthy food ingredients. The identification of an intron/exon-spanning promoter in a hosting gene, resulting in proteins with distinct functions, adds to the complexity of plant genomes.

关键词： ANTISENSE OLIGODEOXYNUCLEOTIDE INHIBITION VACUOLAR INVERTASE GENE LOLIUM-PERENNE L ARABIDOPSIS-THALIANA TRANSCRIPTION FACTOR TREHALOSE 6-PHOSPHATE NICOTIANA-BENTHAMIANA PLANT BIOLOGY LEAF SHEATHS METABOLISM

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sbeIIb基因hpRNA结构植物转化载体的构建

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中国计量学院学报 2006年第4期17卷 337-340页

作者：张明洲方结红陈宗伦刘军胡华军孙传兴 christer jansson 中国计量学院生命科学学院浙江杭州310018 瑞典农业大学植物学系瑞典乌普萨拉SE-75007

hpRNA介导基因沉默技术是一种下调某个内源目的基因表达的有效技术.本研究将淀粉分支酶基因sbeIIb启动子控制下的sbeIIb基因的hpRNA结构构建于植物双元表达载体pCAMBIA1305.1中,并将该重组载体导入农杆菌超毒力菌株AGL1中,构建了一种适... 详细信息

hpRNA介导基因沉默技术是一种下调某个内源目的基因表达的有效技术.本研究将淀粉分支酶基因sbeIIb启动子控制下的sbeIIb基因的hpRNA结构构建于植物双元表达载体pCAMBIA1305.1中,并将该重组载体导入农杆菌超毒力菌株AGL1中,构建了一种适于禾本科的植物转化载体.

关键词： sbeIIb基因载体 hpRNA

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农杆菌介导水稻sbeIIb基因转化体系的建立

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安徽农学通报 2006年第13期12卷 77-78,97页

作者：陈宗伦张明洲方结红刘军俞晓平孙传兴 christer jansson 中国计量学院生命科学学院浙江杭州310018 瑞典农业大学植物学系瑞典乌普萨拉se75007

本文对水稻愈伤组织再生体系及其转化影响因子进行了研究。以水稻品种秀水11的幼胚和成熟胚为外植体,以M S+肌醇0.1g/L+2,4-D 2mg/L+6-BA 0.2mg/L+脯胺酸2.8g/L+水解酪蛋白0.3g/L作诱导培养基,愈伤组织诱导率最高为73.45%,愈伤组织在M S... 详细信息

本文对水稻愈伤组织再生体系及其转化影响因子进行了研究。以水稻品种秀水11的幼胚和成熟胚为外植体,以M S+肌醇0.1g/L+2,4-D 2mg/L+6-BA 0.2mg/L+脯胺酸2.8g/L+水解酪蛋白0.3g/L作诱导培养基,愈伤组织诱导率最高为73.45%,愈伤组织在M S+6-BA2 mg/L+NAA 0.5mg/L的分化培养基上,分化频率达53.33%;在此基础上对农杆菌介导水稻愈伤组织转化的因子进行了优化,获得较高转化频率的条件为:预培养时间3d,在OD600值为0.6农杆菌菌液中感染10m in,共培养3d。

关键词： sbeⅡb基因转化体系农杆菌水稻

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